caryophyllene-oxide and Disease-Models--Animal

An infusion obtained from the leaves of "chal-chal" (Allophylus edulis Radlk.) is used for popular treatment of intestinal disorders and as an anti-inflammatory throat treatment. Because of the anti-inflammatory medicinal folk use, a previous work reported scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015.. The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil. Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.. Leaves were collected from plants at both sites in July 2018. The composition of the essential oil (EOAE-D and EOAE-B) was determined by GC/MS, and major compounds (caryophyllene oxide and α-zingiberene) were isolated and identified by chromatographic methods and NMR spectroscopy. Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).. Both EOAE-D and EOAE-B showed sesquiterpenes as a major constituent, namely, caryophyllene oxide (29.5%) and α-zingiberene (45.0%), respectively. In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.. The results indicate that samples from the two cities differed in chemical composition but not in their anti-inflammatory and antihyperalgesic effects. This finding corroborates the use of A. edulis as a medicinal plant and indicates its potential in the therapy of inflammatory conditions.

Topics: Animals; Anti-Inflammatory Agents; Brazil; Carrageenan; Disease Models, Animal; Hyperalgesia; Inflammation; Male; Mice, Inbred C57BL; Mycobacterium tuberculosis; Oils, Volatile; Pain Threshold; Plant Leaves; Plant Oils; Polycyclic Sesquiterpenes; Polyphenols; Sapindaceae

To observe the analgesic effect and analysis the chemical components of essential oil (EOC) from fresh rhizome of Cyperus rotundus.. Hydrodistillation method was used to extract EOC; Acetic acid induced mice stretching model was applied to evaluate the analgesic effect of EOC. Gas chromatography coupled with mass spectrometry (GC-MS) were applied for the analysis of EOC.. The yield of EOC was 0.4%. The stretching times of mice induced by acetic acid were significantly reduced by EOC at dosage of 0.1 g/kg. 54 ingredients, 85.1% of which were EOC were identified by GC-MS analysis; The major compounds were cyperene (41.03%), beta-caryophyllene oxide (5.32%), alpha-selinene (4.37%), alpha-copaene (4.36%), naphthalene, 6-isoproenyl-4,8a-dimethyl-1, 2, 3, 5, 6, 7, 8, 8a-octahydro-(3.80%) and alpha-cyperone (3.11%).. The essential oil from fresh rhizome of Cyperus rotundus shows structure diversity and good analgesic effect.

Topics: Acetic Acid; Analgesics; Animals; Cyperus; Disease Models, Animal; Drugs, Chinese Herbal; Gas Chromatography-Mass Spectrometry; Mice; Oils, Volatile; Pain; Pain Threshold; Plants, Medicinal; Polycyclic Sesquiterpenes; Rhizome; Sesquiterpenes; Solvents