carbobenzoxy-leucyl-leucyl-norvalinal and Lymphoma--T-Cell

carbobenzoxy-leucyl-leucyl-norvalinal has been researched along with Lymphoma--T-Cell* in 1 studies

Other Studies

1 other study(ies) available for carbobenzoxy-leucyl-leucyl-norvalinal and Lymphoma--T-Cell

ArticleYear
Peptidyl aldehyde inhibitors of proteasome induce apoptosis rapidly in mouse lymphoma RVC cells.
    Journal of biochemistry, 1997, Volume: 121, Issue:3

    Proteases play an important role in regulation of apoptosis. To elucidate the role of proteasome in apoptosis, we examined the effects of the proteasome inhibitors, carbobenzoxy-L-isoleucyl-gamma-t-butyl-L-glutamyl-L-alanyl-L-leucinal and carbobenzoxy-L-leucyl-L-leucyl-L-norvalinal on RVC lymphoma cells. Cells exposed to the proteasome inhibitors arrested at G2/M phase followed by internucleosomal DNA cleavage, chromatin condensation, and formation of apoptotic bodies dose- and time-dependently. Ubiquitinated histone H2A levels decreased in the exposed cells, suggesting a relationship between deubiquitination of histone H2A and the chromatin disarray seen in apoptosis. Northern blots showed an increase in expression of polyubiquitin genes early in the incubation. These findings suggest that the ubiquitin-mediated proteasome-proteolytic system is involved in regulating the cell cycle and apoptosis in RVC cells.

    Topics: Animals; Apoptosis; Cysteine Endopeptidases; Cysteine Proteinase Inhibitors; G2 Phase; Histones; Leupeptins; Lymphoma, T-Cell; Mice; Multienzyme Complexes; Oligopeptides; Proteasome Endopeptidase Complex; RNA, Messenger; Tumor Cells, Cultured; Ubiquitins

1997