capsazepine and Weight-Loss

Published research suggests that activation of transient receptor potential vanilloid subfamily 1 (TRPV1) enhances the expression and deacetylation of peroxisome proliferator-activated receptor gamma (PPARγ) to cause browning of white adipose tissue. Here, we show that TRPV1 activation by capsaicin significantly prevents high fat diet-induced obesity in mice. This is associated with an increase in the expression and deacetylation of PPARγ in the epididymal fat of these mice. Consistent with the TRPV1 activation in vivo, overexpression of TRPV1 enhanced the PPARγ and other thermogenic genes in cultured 3T3-L1 preadipocytes. To determine the interaction between TRPV1 and PPARγ signaling, we analyzed the effect of Troglitazone (Trog; a thiazolidinedione derivative and an agonist of PAARγ) treatment on cultured 3T3-L1 cells. Trog enhanced the expression of TRPV1, PPARγ and thermogenic proteins in undifferentiated 3T3-L1 cells but not in differentiated cells. Acute application of Trog stimulated a robust Ca

Topics: 3T3-L1 Cells; Acetylation; Adipogenesis; Adipose Tissue; Animals; Bone Morphogenetic Proteins; Capsaicin; CCAAT-Enhancer-Binding Proteins; Diet, High-Fat; Epididymis; Feeding Behavior; Ion Channel Gating; Lipid Metabolism; Male; Mice; PPAR alpha; PPAR gamma; RNA, Messenger; Sirtuin 1; Thermogenesis; Troglitazone; TRPV Cation Channels; Uncoupling Protein 1; Weight Loss