capsazepine and Encephalitis

Activated microglial cells generate reactive oxygen species (ROS), which have detrimental effects in neuroinflammatory and neurodegenerative diseases. In the present study, we have identified a novel mechanism involved in microglial NADPH oxidase-mediated ROS production. In PMA-stimulated microglia, ROS production was substantially reduced upon inhibition of the non-selective cation channel TRPV1 with La(3+), ruthenium red, capsazepine and 5-iodo-resinferatoxin. Furthermore, sustained membrane depolarization, a hallmark of NADPH oxidase activity in phagocytes, was found to induce non-selective cation/TRPV1 channel activity in microglia. Together, our data suggest that TRPV1 channels are involved in regulating NADPH oxidase-mediated ROS generation in microglia.

Topics: Animals; Capsaicin; Cell Line; Cell Respiration; Diterpenes; Encephalitis; Gliosis; Ion Channel Gating; Lanthanum; Membrane Potentials; Mice; Microglia; NADPH Oxidases; Organ Culture Techniques; Oxidative Stress; Reactive Oxygen Species; Respiratory Burst; Ruthenium Red; TRPV Cation Channels