cannabidiol and Precursor-Cell-Lymphoblastic-Leukemia-Lymphoma

Cytotoxic effects of cannabidiol (CBD) and tamoxifen (TAM) have been observed in several cancer types. We have recently shown that CBD primarily targets mitochondria, inducing a stable mitochondrial permeability transition pore (mPTP) and, consequently, the death of acute lymphoblastic leukemia (T-ALL) cells. Mitochondria have also been documented among cellular targets for the TAM action. In the present study we have demonstrated a synergistic cytotoxic effect of TAM and CBD against T-ALL cells. By measuring the mitochondrial membrane potential (ΔΨm), mitochondrial calcium ([Ca

Topics: Calcium; Cannabidiol; Cell Line, Tumor; Drug Synergism; Homeostasis; Humans; Jurkat Cells; Membrane Potential, Mitochondrial; Mitochondrial Permeability Transition Pore; Models, Molecular; Molecular Docking Simulation; Peptidyl-Prolyl Isomerase F; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Protein Conformation; Tamoxifen

Anticancer activity of different phenols is documented, but underlying mechanisms remain elusive. Recently, we have shown that cannabidiol kills the cells of acute lymphoblastic leukemia (ALL) by a direct interaction with mitochondria, with their consequent dysfunction. In the present study, cytotoxic effects of several phenolic compounds against human the T-ALL cell line Jurkat were tested by means of resazurin-based metabolic assay. To unravel underlying mechanisms, mitochondrial membrane potential (∆Ψ

Topics: Antineoplastic Agents, Phytogenic; Cannabidiol; Curcumin; Drug Screening Assays, Antitumor; Humans; Jurkat Cells; Membrane Potential, Mitochondrial; Mitochondria; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Quercetin; Reactive Oxygen Species

Anticancer properties of non-psychoactive cannabinoid cannabidiol (CBD) have been demonstrated on tumors of different histogenesis. Different molecular targets for CBD were proposed, including cannabinoid receptors and some plasma membrane ion channels. Here we have shown that cell lines derived from acute lymphoblastic leukemia of T lineage (T-ALL), but not resting healthy T cells, are highly sensitive to CBD treatment. CBD effect does not depend on cannabinoid receptors or plasma membrane Ca

Topics: Calcium; Calcium Signaling; Cannabidiol; Cannabinoids; Cell Death; Cell Line, Tumor; Homeostasis; Humans; Mitochondria; Precursor Cell Lymphoblastic Leukemia-Lymphoma; T-Lymphocytes