candicidin and Carcinoma--Ehrlich-Tumor

The fractional composition of chromatin proteins and an electron-microscopic pattern of cell ultrastructure of the Ehrlich ascite tumours and ascite lymphoma NK/Ly were studied when suppressing their growth by a combined action of cyclophosphane and polyene antibiotic. Changes in the fractional composition of chromatin proteins were found under the effect of levorin. A sharp increase in this effect and summation of heterogeneous morphological changes provoked by each of the preparation occur under a combined application of the preparations.

Topics: Animals; Antifungal Agents; Candicidin; Carcinoma, Ehrlich Tumor; Chromatin; Cyclophosphamide; Drug Therapy, Combination; Female; Histones; Lymphoma; Male; Mice; Neoplasm Proteins

The polyene antibiotic levorin had no effect on the incorporation of 14C-uracil into the pool of intracellular free nucleotides in Ehrlich ascites tumour cells. RNA synthesis was inhibited by the antibiotic only at the late stages of incubation, evidently, due to earlier disturbances of the protein synthesis and in cell energetics.

Topics: Animals; Antifungal Agents; Candicidin; Carcinoma, Ehrlich Tumor; Cells, Cultured; Mice; RNA, Neoplasm; Uracil

Levorene, a polyenic antibiotic, lowered the concentration of amino acids in the cells of Ehrlich carcinoma. The decrease in the intracellular level of the amino acids was due not only to inhibition of their entrance to the cells but also to their increased leaching from the cells. The effect of levorene on the intracellular level of the neutral amino acids was higher than that on the main amino acids which was associated with different sensitivity of the amino acid transport systems to the antibiotic.

Topics: Amino Acids; Animals; Antifungal Agents; Biological Transport; Candicidin; Carcinoma, Ehrlich Tumor; Cells, Cultured; Time Factors

A polyenic antibiotic levorin had a significant damaging effect on the amino acid transport in the cells of Ehrlich's ascites carcinoma by inhibiting the incorporation and increasing the release of 14C-glycine and 14C-arginine. Inhibiton of incorporation of the radioactive amino acids into protein was observed on both simultaneous addition of the antibiotic and the label into the medium and addition of previously labeled cells into radioinactive medium with the antibiotic. Since in the latter case levorin had probably no effect on the specific radioactivity of the precursor, it was suggested that the decrease in the label incorporation into protein was an evidence of the real antibiotic inhibition of the rate of the synthesis of the polypeptide chain.

Topics: Amino Acids; Animals; Antifungal Agents; Biological Transport; Candicidin; Carbon Radioisotopes; Carcinoma, Ehrlich Tumor; Free Radicals; In Vitro Techniques; Neoplasm Proteins; Peptides; Time Factors

Five-minute incubation of the cells of Ehrlich's mouse carcinoma with levorin in a concentration of 0.25 microgram/ml resulted in a release of 30% of K+ and 40-50% suppression of active transport of neutral amino acids. No changes in incorporation of nitrous compounds, nucleosides and cyclophosphamide, an antitumor drug transported by the systems of facilitated diffusion were observed under the same conditions.

Topics: Amino Acids; Animals; Antifungal Agents; Biological Transport; Candicidin; Carcinoma, Ehrlich Tumor; Cell Membrane Permeability; Cyclophosphamide; Diffusion; In Vitro Techniques; Mice; Nucleosides

Topics: Animals; Antifungal Agents; Biological Transport; Candicidin; Carcinoma, Ehrlich Tumor; Cell Membrane Permeability; Cells, Cultured; Culture Media; Depression, Chemical; Glycine; Leucine; Mice

The fractional composition of the proteins of the plasmatic membranes of the Ehrlich's tumor cells treated with various drugs was studied with the use of electrophoresis in polyacrylamide gel. It was shown that the character of the changes in the fractional composition of the plasmatic membrane proteins under the effect of levorin and cyclophosphamide was different. When the drugs were used in combination, summation of the changes induced by every drug alone was observed. Possible causes of the changes in the fractional composition of the membrane proteins are discussed.

Topics: Animals; Antifungal Agents; Candicidin; Carcinoma, Ehrlich Tumor; Cyclophosphamide; Drug Evaluation, Preclinical; Drug Synergism; Drug Therapy, Combination; Membrane Proteins; Mice; Neoplasm Proteins