calpain and Urinary-Incontinence--Stress

The aim of the present study was to investigate the expression and mechanism of microRNA (miR)‑93 in collagen expression in stress urinary incontinence (SUI). Vaginal tissue, primary fibroblasts and SUI primary fibroblasts were obtained to detect the expression of miR‑93, interstitial collagenase (MMP1), collagen I and calpain‑2. Reverse transcription‑quantitative polymerase chain reaction analysis was performed to detect the levels of miR‑93 and MMP1. Western blotting was used to evaluate the protein levels of calpain‑2, MMP1 and collagen I. MMP1 and hydroxyproline levels in the supernatant were measured by ELISA. The association between miR‑93 and calpain‑2 was investigated by luciferase reporter assays. The expression of miR‑93 and collagen I was significantly downregulated in the SUI group, while the expression of calpain‑2 and MMP1 was significantly upregulated. ELISA analysis demonstrated that the MMP1 level increased and the hydroxyproline level decreased in the SUI group. Additionally, calpain‑2 was identified to be a target of miR‑93, and miR‑93 was able to negatively regulate the expression of calpain‑2. Restoration of calpain‑2 in miR‑93‑overexpresseing SUI primary fibroblasts reversed the alteration in hydroxyproline expression, indicating that calpain‑2 was negatively associated with collagen expression. The results of the present study suggested that miR‑93 regulated MMP1 and collagen I expression in fibroblasts via calpain‑2. miR‑93 mediated collagen expression in stress urinary incontinence via calpain‑2.

Topics: Calpain; Collagen Type I; Female; Fibroblasts; Gene Expression Regulation; Humans; Matrix Metalloproteinase 1; MicroRNAs; Middle Aged; Urinary Incontinence, Stress; Vagina

The objective of this study is to investigate the expression of calpain-1, calpain-2, and calpastatin in the human periurethral vaginal tissues and to show the potential link between calpain system and stress urinary incontinence (SUI).. The periurethral vaginal tissues of 39 women with SUI and 31 women without SUI were collected to detect the expressions of calpains and calpastatin by using semi-quantitative competitive reverse transcription-polymerase chain reaction and Western blotting.. There were no significant differences on the expressions of calpain-1 at the levels of messenger RNA (mRNA) and protein in both groups (P > 0.05), but the patients with SUI had significantly higher levels of calpain-2 mRNA and protein than the control (P < 0.05); and the mRNA expressions of calpastatin in women with SUI were significantly higher than the control (P < 0.05), while the protein expressions were significantly lower when compared to the control (P < 0.01).. Overexpression of calpain-2 and low expression of calpastatin may involve in the pathological development of SUI.

Topics: Calcium-Binding Proteins; Calpain; Case-Control Studies; Female; Humans; Middle Aged; RNA, Messenger; Urinary Incontinence, Stress; Vagina