calpain has been researched along with Retroperitoneal-Neoplasms* in 1 studies
1 other study(ies) available for calpain and Retroperitoneal-Neoplasms
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E-Cadherin fragments as potential mediators for peritoneal metastasis in advanced epithelial ovarian cancer.
Peritoneal dissemination and retroperitoneal lymph node involvement are main routes for tumour spread of epithelial ovarian cancer (EOC), possibly determined by the intercellular connecting protein E-Cadherin (E-Cad) and its fragments.. Tumour tissue of 105 advanced EOC patients was evaluated for protein expression of E-Cad, β-Catenin and Calpain by western blotting and immunohistochemistry. Expression patterns were compared between tumours with solely intraperitoneal (pT3c, pN0; n=41) and tumours with retroperitoneal metastases (pT1a-3c, pN1; n=64). Lysates of the EOC cell line SKOV3 and tumour tissue from the intraperitoneal group were tested for E-Cad expression following Calpain treatment.. E-Cad full-length (E-Cad-FL, 120 kDa) and two major fragments at 85 kDa (E-Cad-85) and 23 kDa (E-Cad-23) were detected by western blotting. E-Cad-85 expression was significantly higher in tumours with solely intraperitoneal metastases and correlated strongly with E-Cad-23 and the protease Calpain. Calpain-mediated cleavage was identified as a potential mechanism to generate E-Cad-85 from E-Cad-FL by treating lysates from SKOV3 cells and tumour tissue with this enzyme. Increased cytoplasmic localisation of β-Catenin in tumours with high E-Cad-85 expression corroborates that E-Cad-85 loses the binding site for β-Catenin after fragmentation, enabling tumour cluster formation and peritoneal dissemination.. Calpain-mediated E-Cad fragmentation appears to promote intraperitoneal EOC progression. Understanding these mechanisms might eventually lead to new tailored subtype-specific diagnostic and therapeutic interventions. Topics: Adult; Aged; Aged, 80 and over; beta Catenin; Blotting, Western; Cadherins; Calpain; Carcinoma, Ovarian Epithelial; Cell Line, Tumor; Female; Humans; Immunohistochemistry; Lymph Nodes; Middle Aged; Neoplasms, Glandular and Epithelial; Ovarian Neoplasms; Peptide Fragments; Peritoneal Neoplasms; Retroperitoneal Neoplasms; Retroperitoneal Space; Retrospective Studies | 2016 |