calicheamicin-t and Neoplasms

calicheamicin-t has been researched along with Neoplasms* in 1 studies

Other Studies

1 other study(ies) available for calicheamicin-t and Neoplasms

Article	Year
In-solution staining and arraying method for the immunofluorescence detection of γH2AX foci optimized for clinical applications. BioTechniques, 2011, Volume: 51, Issue:3 Immunofluorescence quantification of γH2AX foci is a powerful approach to quantify DNA double-strand breaks induced by cancer therapy or accidental exposure to ionizing radiation. Here we report a modification to the γH2AX immunofluorescence labeling method, whereby cells are stained in-solution before being spotted and fixed onto microscope slides. Our modified method allows arraying of 16 patient samples/slide ready for foci counting in 2 h and demonstrated reliably detection of γH2AX foci in mononuclear cells prepared from patients who had undergone radiation therapy. Topics: Aminoglycosides; DNA Breaks, Double-Stranded; Enediynes; Fibroblasts; Fluorescent Antibody Technique; Histones; Humans; Leukocytes, Mononuclear; Neoplasms; Staining and Labeling; Tissue Array Analysis	2011

Article

Year

In-solution staining and arraying method for the immunofluorescence detection of γH2AX foci optimized for clinical applications.

BioTechniques, 2011, Volume: 51, Issue:3

Immunofluorescence quantification of γH2AX foci is a powerful approach to quantify DNA double-strand breaks induced by cancer therapy or accidental exposure to ionizing radiation. Here we report a modification to the γH2AX immunofluorescence labeling method, whereby cells are stained in-solution before being spotted and fixed onto microscope slides. Our modified method allows arraying of 16 patient samples/slide ready for foci counting in 2 h and demonstrated reliably detection of γH2AX foci in mononuclear cells prepared from patients who had undergone radiation therapy.

Topics: Aminoglycosides; DNA Breaks, Double-Stranded; Enediynes; Fibroblasts; Fluorescent Antibody Technique; Histones; Humans; Leukocytes, Mononuclear; Neoplasms; Staining and Labeling; Tissue Array Analysis

2011