calcimycin and Mouth-Neoplasms

A new cell line (CoMS) was established from a 3-year-old male mongrel dog with mast cell tumor of the oral mucosa. CoMS cells grow in suspension with a doubling time of 27.0 +/- 0.7 hr. The cytoplasmic granules were formalin-sensitive, showed diverse appearances in their ultrastructural findings and contained heparin proteoglycan and neutral protease chymase. Calcium ionophore A23187, substance P and concanavalin A caused significant histamine release from CoMS cells, while compound 48/80 failed to release histamine. This cell line will make an available source for studies on canine mast cell tumors.

Topics: Animals; Antibodies, Anti-Idiotypic; Calcimycin; Cell Division; Concanavalin A; Dog Diseases; Dogs; Histamine; Histocytochemistry; Ionophores; Male; Mastocytosis; Microscopy, Electron; Mouth Neoplasms; p-Methoxy-N-methylphenethylamine; Substance P; Tumor Cells, Cultured