calcimycin and Carcinoma--Embryonal

In a previous report, we reported that the induction of GM-CSF gene in differentiated P19 cells results from the maturation of the transcriptional machinery. Here, we identified a cis-DNA element which confers the activation of GM-CSF gene in response to PMA/A23187 stimulation in differentiated state. Analysis of the 5'-flanking region between -113 and -60 revealed two elements responsible for promotion and one for inhibition, and the overall effects led to the activation of GM-CSF gene mainly through the sequence between -95 and -73. Using the oligonucleotide between -94 and -73 as a probe in gel retardation assays, we identified a DNA-binding protein, NF-GM-P19, the binding activity of which was induced after differentiation in response to PMA/A23187 stimulation. These results indicate that the induction of GM-CSF gene after differentiation results from the maturation of the transcriptional machinery which recognizes the sequence between -95 and -73.

Topics: Animals; Base Sequence; Calcimycin; Carcinoma, Embryonal; Cell Differentiation; DNA Mutational Analysis; DNA-Binding Proteins; DNA, Neoplasm; Gene Expression Regulation, Neoplastic; Granulocyte-Macrophage Colony-Stimulating Factor; Mice; Molecular Sequence Data; Protein Binding; Regulatory Sequences, Nucleic Acid; Signal Transduction; Tetradecanoylphorbol Acetate; Transfection; Tumor Cells, Cultured