calcein-am and Glioma

calcein-am has been researched along with Glioma* in 3 studies

Other Studies

3 other study(ies) available for calcein-am and Glioma

ArticleYear
P-glycoprotein inhibition of drug resistant cell lines by nanoparticles.
    Drug development and industrial pharmacy, 2016, Volume: 42, Issue:2

    Several pharmaceutical excipients are known for their ability to interact with cell membrane lipids and reverse the phenomenon of multidrug resistance (MDR) in cancer. Interestingly, many excipients act as stabilizers and are key ingredients in a variety of nano-formulations. In this study, representatives of ionic and non-ionic excipients were used as surface active agents in nanoparticle (NP) formulations to utilize their MDR reversing potential. In-vitro assays were performed to elucidate particle-cell interaction and accumulation of P-glycoprotein (P-gp) substrates-rhodamine-123 and calcein AM, in highly drug resistant glioma cell lines. Chemosensitization achieved using NPs and their equivalent dose of free excipients was assessed with the co-administered anti-cancer drug doxorubicin. Among the excipients used, non-ionic surfactant, Cremophor® EL, and cationic surfactant, cetyltrimethylammonuium bromide (CTAB), demonstrated highest P-gp modulatory activity in both free solution form (up to 7-fold lower IC50) and as a formulation (up to 4.7-fold lower IC50) as compared to doxorubicin treatment alone. Solutol® HS15 and Tween® 80 exhibited considerable chemosensitization as free solution but not when incorporated into a formulation. Sodium dodecyl sulphate (SDS)-based nanocarriers resulted in slightly improved cytotoxicity. Overall, the results highlight and envisage the usage of excipient in nano-formulations in a bid to improve chemosensitization of drug resistant cancer cells towards anti-cancer drugs.

    Topics: Animals; Antibiotics, Antineoplastic; ATP Binding Cassette Transporter, Subfamily B, Member 1; Cell Line, Tumor; Chemistry, Pharmaceutical; Doxorubicin; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Excipients; Fluoresceins; Glioma; Inhibitory Concentration 50; Nanoparticles; Rats; Rhodamine 123; Surface-Active Agents

2016
Observations on the viability of C6-glioma cells after sonoporation with low-intensity ultrasound and microbubbles.
    IEEE transactions on ultrasonics, ferroelectrics, and frequency control, 2013, Volume: 60, Issue:1

    Ultrasound (US) and microbubbles can be used to facilitate cellular uptake of drugs through a cavitationinduced enhancement of cell membrane permeability. The mechanism is, however, still incompletely understood. A direct contact between microbubbles and cell membrane is thought to be essential to create membrane perturbations lasting from seconds to minutes after US exposure of the cells. A recent study showed that the effect may even last up to 8 h after cavitation (with residual permeability up to 24 h after cavitation). In view of possible membrane damage, the purpose of this study was to further investigate the evolution of cell viability in the range of the 24-h temporal window. Furthermore, a description of the functional changes in tumor cells after US exposure was initiated to obtain a better understanding of the mechanism of membrane perturbation after sonication with microbubbles. Our results suggest that US does not reduce cell viability up to 24 h post-exposure. However, a perturbation of the entire cell population exposed to US was observed in terms of enzymatic activity and characteristics of the mitochondrial membrane. Furthermore, we demonstrated that US cavitation induces a transient loss of cell membrane asymmetry, resulting in phosphatidylserine exposure in the outer leaflet of the cell membrane.

    Topics: Acridine Orange; Animals; Annexin A5; Carbocyanines; Cell Line, Tumor; Cell Membrane; Cell Survival; Fluoresceins; Fluorescent Dyes; Glioma; Microbubbles; Microscopy, Fluorescence; Rats; Sonication; Tetrazolium Salts; Thiazoles

2013
Cytotoxic actions of the heavy metal chelator TPEN on NG108-15 neuroblastoma-glioma cells.
    Neurotoxicology, 1999, Volume: 20, Issue:4

    Effects of the heavy metal chelator N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) were investigated on cytotoxicity in clonal NG108-15 neuroblastoma-glioma hybrid cells. Three min after addition of 100 microM TPEN, cells began to retract their neurites and lose their characteristic multipolar shape; by 3-4 hr of exposure, most cells detached from the substrate, either singly or as variable-sized aggregates. Viability was assessed by monitoring uptake of calcein AM and propidium iodide, fluorescent dyes that served as markers for live and dead cells, respectively. Incubation of cultures in 100 microM TPEN led to a gradual decrease in the population exhibiting calcein fluorescence (viable cells) and a corresponding increase in the population displaying propidium iodide fluorescence (nonviable cells). Loss of cell viability reached 12% at 8 hr, 61% at 24 hr and 83% by 48 hr. Ultrastructural examination of TPEN-treated cells revealed condensed chromatin and fragmented nuclei, characteristic of apoptosis, as well as plasma membrane defects and organelle swelling, generally associated with necrosis. Addition of an equimolar concentration of Zn2+ or Cu2+ but not Fe2+ or Mn2+ prevented morphological abnormalities and cell death.

    Topics: Animals; Apoptosis; Biomarkers; Cell Aggregation; Cell Nucleus; Cell Survival; Chelating Agents; Chromatin; Copper; Ethylenediamines; Fluoresceins; Fluorescent Dyes; Glioma; Iron; Manganese; Metals, Heavy; Mice; Microscopy, Electron, Scanning; Necrosis; Neuroblastoma; Propidium; Rats; Time Factors; Tumor Cells, Cultured; Zinc

1999