calca-protein--human and Periodontal-Pocket

There is an ongoing controversy on the benefits of treatment protocols, including dental lasers and photodynamic therapy (PDT). The purpose of this study is to compare the local biologic effects of PDT, diode soft laser (DSL) therapy, and conventional deep scaling and root planing (SRP) in residual pockets.. Thirty-two individuals were included based on a history of previous treatment for periodontitis and the persistence of sites with probing depths >4 mm and bleeding on probing. Residual pockets were debrided with an ultrasonic device and then randomly assigned either to PDT, DSL, or SRP. Gingival crevicular fluid was collected before treatment, after 14 days, and at 2 and 6 months. Levels of 13 cytokines and nine acute-phase proteins were measured using a bead-based multiplexing analysis system.. Treatment with PDT, DSL, or SRP led to significant changes in several cytokines and acute-phase proteins: Compared with baseline, levels of interleukin-17, basic fibroblast growth factor, granulocyte colony-stimulating factor, granulocyte macrophage colony-stimulating factor, and macrophage inflammatory protein 1-α were lower 14 days and 2 months after treatment. Except for granulocyte colony-stimulating factor, these differences remained significant throughout the observation period. The levels of five acute-phase proteins (α-2 macroglobulin, haptoglobin, serum amyloid P, procalcitonin, and tissue plasminogen activator) were significantly higher at 6 months than at baseline. No significant differences were observed among the three treatment modalities at any time point for any biochemical parameter.. Levels of several cytokines and acute-phase proteins significantly changed after treatment regardless of treatment modality. There was no evidence for a specific DSL- or PDT-enhanced expression of inflammatory mediators.

Topics: Acute-Phase Proteins; Adult; Aged; alpha-Macroglobulins; Calcitonin; Calcitonin Gene-Related Peptide; Chemokine CCL3; Cytokines; Dental Scaling; Female; Fibroblast Growth Factor 2; Follow-Up Studies; Gingival Crevicular Fluid; Glycoproteins; Granulocyte Colony-Stimulating Factor; Haptoglobins; Humans; Interleukin-17; Lasers, Semiconductor; Male; Middle Aged; Periodontal Pocket; Periodontitis; Photochemotherapy; Protein Precursors; Serum Amyloid P-Component; Tissue Plasminogen Activator; Treatment Outcome; Ultrasonic Therapy

Vitamin D-binding protein (DBP) is a multifunctional and highly expressed plasma protein. Among its diverse roles, including those in the immune and inflammatory responses, it is the primary carrier of vitamin D, which has been implicated in periodontitis. We hypothesized that there is a correlation between systemic DBP levels and generalized aggressive periodontitis (GAgP).. Forty-four patients with GAgP and 32 healthy controls were recruited. Clinical parameters were examined, including the mean bleeding index, probing depth, attachment loss and percentage of severely affected sites. Blood chemistry analyses were performed for each subject. Plasma levels of DBP, interleukin-6 (IL-6) and procalcitonin (PCT) were measured using ELISAs, and plasma levels of 25-hydroxy-vitamin D(3) (25(OH)D(3)) were detected using a radioimmunoassay.. Significantly higher levels of plasma DBP, IL-6, PCT and 25(OH)D(3), as well as leukocyte counts, neutrophil counts and neutrophil percentages were found in patients with GAgP compared with healthy controls (p < 0.05 for all). Multiple linear regression analysis showed that the plasma DBP levels were significantly correlated with GAgP, plasma PCT levels and smoking status (p < 0.05 for all). In the GAgP group, the plasma DBP levels in smokers were significantly higher than those in nonsmokers (p < 0.001).. Elevated plasma vitamin DBP levels are associated with GAgP.

Topics: Adult; Aggressive Periodontitis; Calcifediol; Calcitonin; Calcitonin Gene-Related Peptide; Female; Gingival Hemorrhage; Humans; Inflammation Mediators; Interleukin-6; Leukocyte Count; Male; Neutrophils; Periodontal Attachment Loss; Periodontal Index; Periodontal Pocket; Protein Precursors; Smoking; Vitamin D-Binding Protein; Young Adult