caesium-137 and Leukemia--Lymphocytic--Chronic--B-Cell

to establish the level of chromosomal instability in peripheral blood lymphocytes (PBL) from healthyindividuals and cells from patients with B-cell chronic lymphocytic leukemia (CLL) under co-cultivation conditions and to determine the possibility of inducing manifestations of the universal phenomenon of bystander response in them.. Cytogenetic analysis of uniformly stained chromosomes from human PBL, which differed by cytogenetic markers of sex; the presence of oncological transformation and in vitro irradiation of 137Cs in a dose of 0.50 Gy under the conditions of their joint cultivation was performed.. The frequency of chromosome aberrations in PBL from healthy individuals when co-cultured with the blood from CLL patients was 3.35 per 100 cells, exceeded the control (1.48 per 100 cells, р < 0.01), did not significantly differ from the rate in non-irradiated blood T-lymphocytes from patients with B-cell CLL (3.18 per 100 cells, р > 0.05) and was lower than when co-cultivated with irradiated blood from CLL patients (5.00 per 100 cells, р < 0.01). In irradiated in vitro blood lymphocytes from CLL patients, the mean group level of chromosome aberrations under separate cultivation was 12.36 per 100 cells and exceeded the indicator during their co-cultivation with lymphocytes from healthy individuals (8.35 per 100 cells, р < 0.05).. A tumor-induced bystander effect (TIBE) develops in PBL from healthy individuals when co-cultured with the blood from CLL patients and in T lymphocytes of B-cell CLL patients, the cytogenetic manifestation of which is an increase in the frequency of single chromatid fragments. The interaction of irradiated blood cells from CLL patients with lymphocytes from healthy individuals causes an increase in chromosomal instability in the latter due to the development of a radiation-induced bystander effect (RIBE) and leads to a decrease in the level of chromosomal instability in irradiated lymphocytes from CLL patients (rescue effect). An increase in the level of chromatid-type aberrations in T-lymphocytes of CLL patients during in vitro irradiation is a consequence of the development of RIBE against the background of TIBE.. Meta: vstanovyty riven' khromosomnoï nestabil'nosti v limfotsytakh peryferychnoï krovi (LPK) zdorovykh osib ta klitynakh khvorykh na V-klitynnu khronichnu limfotsytarnu leĭkemiiu (KhLL) za umov ïkh sumisnogo kul'tyvuvannia ta vyznachyty mozhlyvist' induktsiï v nykh proiaviv universal'nogo iavyshcha efektu svidka.Materialy i metody. Doslidzhennia vykonane z zastosuvanniam tsytogenetychnogo analizu rivnomirno zabarvlenykh khromosom LPK liudyny, shcho rozriznialys' za tsytogenetychnymy markeramy stati; naiavnistiu onkologichnoï transformatsiï ta oprominennia in vitro 137Cs v dozi 0,50 Gr za ïkh sumisnogo kul'tyvuvannia.Rezul'taty. Chastota aberatsiĭ khromosom u LPK zdorovykh osib za kul'tyvuvannia z krov’iu khvorykh na KhLL skladala 3,35 na 100 metafaz, perevyshchuvala kontrol'nu (1,48 na 100 klityn, r < 0,01), znachushche ne vidriznialas' vid pokaznyka u neoprominenykh T-limfotsytakh krovi khvorykh na V-klitynnu KhLL (3,18 na 100 klityn, r > 0,05) i bula nyzhchoiu, nizh pry ko-kul'tyvuvanni z oprominenoiu krov’iu khvorykh na KhLL (5,00 na 100 klityn, r < 0,01). V oprominenykh in vitro limfotsytakh krovi khvorykh na KhLL seredn'ogrupovyĭ riven' aberatsiĭ khromosom pry okremomu kul'tyvuvanni stanovyv 12,36 na 100 metafaz i perevyshchuvav pokaznyk za ko-kul'tyvuvannia z limfotsytamy zdorovykh osib (8,35 na 100 klityn, r < 0,05). Vysnovky. U LPK zdorovykh osib za kul'tyvuvannia z krov’iu khvorykh na KhLL ta u T-limfotsytakh khvorykh na V-klitynnu KhLL rozvyvaiet'sia pukhlynno-indukovanyĭ efekt svidka, tsytogenetychnym proiavom iakogo ie zrostannia chastoty odynochnykh khromatydnykh fragmentiv. Vzaiemodiia oprominenykh klityn krovi khvorykh na V-klitynnu KhLL z intaktnymy limfotsytamy zdorovykh osib sprychyniaie v ostannikh zbil'shennia khromosomnoï nestabil'nosti vnaslidok rozvytku radiatsiĭno-indukovanogo efektu svidka ta pryzvodyt' do znyzhennia rivnia khromosomnoï nestabil'nosti v oprominenykh limfotsytakh khvorykh na KhLL (efektu poriatunku). Pidvyshchenyĭ riven' aberatsiĭ khromatydnogo typu v T-limfotsytakh khvorykh na KhLL pry oprominenni in vitro ie rezul'tatom rozvytku v nykh radiatsiĭno-indukovanogo efektu svidka na tli onkologichno-indukovanogo efektu svidka.

Topics: Cesium Radioisotopes; Chromatids; Chromosomal Instability; Chromosome Aberrations; Cytogenetic Analysis; Dose-Response Relationship, Radiation; Humans; Leukemia, Lymphocytic, Chronic, B-Cell; Lymphocytes

to determine the impact of the irradiated in vitro blood cells from patients with B-cell chronic lymphocytic leukemia (CLL) on the level of chromosomal instability in peripheral blood lymphocytes (PBL) from healthy persons during the development of tumor-induced bystander effect.. Separate and joint cultivation of PBL from healthy persons (cells-bystanders) together withblood cells from CLL patients irradiated in vitro at the G0 stage of the mitotic cycle by γ-quanta 137Cs in a dose of0.5 Gy 137Cs (cells-inductors) was used. For joint cultivation our own model system for co-cultivation of PBL fromindividuals of different sex, designed by us to investigate the bystander effects at the cytogenetic level was used.Traditional cytogenetic analysis of uniformly painted chromosomes with group karyotyping was performed. The frequency of chromosome aberrations in cells-inductors and cells-bystanders as the markers of chromosome instability were determined.. Found that at co-cultivation of PBL from healthy individuals with irradiated blood cells from CLL patientsthe middle group frequency of chromosome aberrations in the bystander cells (5.18 ± 0.51 per 100 metaphases,p < 0.001) was statistically significant higher than its background level determined at a separate cultivaton (1.52± 0.30 per 100 metaphases), and at co-cultivation with non-irradiated blood cells from CLL patients (3.31 ± 0.50 per100 metaphases, p < 0.01).. Co-cultivation of in vitro irradiated blood cells from CLL patients with PBL from healthy persons leadsto an increase in the level of chromosome instability in the bystander cells due to synergism between tumor-inducedand radiation-induced bystander effects.. Meta: vyznachyty vplyv oprominenykh in vitro klityn krovi patsiientiv z V-klitynnoiu khronichnoiu limfotsytarnoiu leĭkemiieiu (KhLL) na riven' khromosomnoï nestabil'nosti v limfotsytakh peryferychnoï krovi (LPK) zdorovykh osib pryrozvytku pukhlyno-indukovanogo efektu svidka.Materialy i metody. Zastosovuvaly okreme ta spil'ne kul'tyvuvannia LPK zdorovykh osib (klityny-svidky) z klitynamy krovi patsiientiv z KhLL, oprominenymy in vitro na G0 stadiï klitynnogo tsyklu γ-kvantamy 137Cs v dozi 0,5 Gr(klityny-induktory). Dlia sumisnogo kul'tyvuvannia klityn vykorystovuvaly vlasnu model'nu systemu spil'nogokul'tyvuvannia LPK riznostatevykh osib, rozroblenu dlia doslidzhennia efektu svidka na tsytogenetychnomu rivni.Provodyly analiz rivnomirno zabarvlenykh khromosom z grupovym kariotypuvanniam. Vyznachaly chastotu khromosomnykh aberatsiĭ v klitynakh-induktorakh ta klitynakh-svidkakh.Rezul'taty. Vstanovyly, shcho pry sumisnomu kul'tyvuvanni LPK zdorovykh osib z oprominenymy klitynamy krovipatsiientiv z KhLL seredn'ogrupova chastota aberatsiĭ khromosom v klitynakh-svidkakh (5,18 ± 0,51 na 100 metafaz)statystychno znachushche perevyshchyla iak ïkh fonovyĭ riven', vyznachenyĭ pry okremomu kul'tyvuvanni (1,52 ± 0,30 na100 metafaz, p < 0,001)), tak i tseĭ pokaznyk pry ko-kul'tyvuvanni klityn-svidkiv z neoprominenymy klitynamykrovi patsiientiv z KhLL (3,31 ± 0,50 na 100 metafaz, p < 0,01).Vysnovky. Sumisne kul'tyvuvannia oprominenykh in vitro klityn krovi patsiientiv z V-klitynnoiu KhLL z limfotsytamyperyferychnoï krovi umovno zdorovykh osib pryzvodyt' do pidvyshchennia rivnia khromosomnoï nestabil'nosti vklitynakh-svidkakh vnaslidok synergizmu mizh pukhlyno-indukovanym ta radiatsiĭno- indukovanym efektamy svidka.

Topics: Adult; Aged; Bystander Effect; Cell Communication; Cesium Radioisotopes; Chromosomal Instability; Chromosome Aberrations; Coculture Techniques; Female; Gamma Rays; Healthy Volunteers; Humans; Leukemia, Lymphocytic, Chronic, B-Cell; Leukocytes, Mononuclear; Lymphocytes; Male; Metaphase; Middle Aged; Primary Cell Culture