cadinol and Neoplasms

This study aimed to evaluate the variations of the chemical composition and bioactivity of essential oils of Liquidambar styraciflua L. (Altingiaceae) collected in different seasons.. The oils were analysed by GLC/FID and GLC/MS. The antioxidant activity was investigated by diphenylpicrylhydrazyl (DPPH) and superoxide anion radical scavenging assays and the deoxyribose degradation assay. Inhibition of both 5-lipoxygenase (5-LOX) and prostaglandin E2 (PGE2) production in hepatic cancer (HepG-2) cells were used to assess the anti-inflammatory activity. The cytotoxic activity was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.. Altogether, 64 volatile secondary metabolites were identified. The major components of the leaf oil were d-limonene, α-pinene and β-pinene, and of the stem oil were germacrine D, α-cadinol, d-limonene, α-pinene, and β-pinene. Leaf and stem oils collected in spring could reduce DPPH● (IC50 = 3.17 and 2.19 mg/ml) and prevent the degradation of the deoxyribose sugar (IC50 = 17.55 and 14.29 μg/ml). The stem oil exhibited a higher inhibition of both 5-LOX and PGE2 than the leaf oil. The cytotoxic activity of leaf and stem oils was low in cancer cell lines (IC50 = 136.27 and 119.78 μg/ml in cervical cancer (HeLa) cells).. Essential oils of L. styraciflua exhibited an interesting anti-inflammatory activity with low cytotoxicity, supporting its traditional use to treat inflammation.

Topics: Anti-Inflammatory Agents; Antioxidants; Arachidonate 5-Lipoxygenase; Bicyclic Monoterpenes; Biphenyl Compounds; Bridged Bicyclo Compounds; Cyclohexenes; Deoxyribose; Dinoprostone; HeLa Cells; Hep G2 Cells; Humans; Limonene; Liquidambar; Monoterpenes; Neoplasms; Oils, Volatile; Picrates; Plant Extracts; Plant Leaves; Plant Stems; Sesquiterpenes, Germacrane; Superoxides; Terpenes

Three monoterpenoids and two triterpenoids were isolated from Abies nephrolepis together with 53 known terpenoids. The structures of the compounds were established by 1D and 2D NMR spectroscopy. The absolute configuration of 3-hydroxycamphane-2-carboxylic acid was established as (1S,2R,3S,4R) by Cu-Kα X-ray crystallography. All 58 isolates were tested for cytotoxic activity against four tumor cells viz. A549 (human lung adenocarcinoma), Colo205 (colon adenocarcinoma), QGY-7703 (human hepatoma) and THP-1 (human monocytic leukemia). α-Cadinol exhibited the best effects on A549, Colo205 and QGY-7703 with IC(50) values of 8.6, 8.1 and 4.6 μg/mL, respectively.

Topics: Abies; Antineoplastic Agents, Phytogenic; Camphanes; Cell Line, Tumor; Humans; Inhibitory Concentration 50; Molecular Structure; Neoplasms; Phytotherapy; Plant Extracts; Terpenes