c.i.-fluorescent-brightening-agent-28 and Candidiasis--Oral

Denture-related stomatitis (DRS) is still a dilemma in removable prosthodontics. The aim of this study was to investigate the relationship of DRS with the presence of Candida albicans hyphae and Lactobacillus. A total of 91 patients wearing maxillary and mandibular complete dentures were included in the present study and tested mycologically as well as bacteriologically. A statistically significant association of DRS was found with denture age (p = 0.003) and continuous denture wearing (p = 0.015). Presence of C. albicans hyphae was shown to be significantly higher in DRS cases (p < 0.01), and there was a statistically significant positive correlation between presence of hyphae and C. albicans (p < 0.01). Another interesting finding was that DRS patients showed higher Lactobacillus counts in their saliva (p = 0.04), as well as in the palate (p = 0.028). C. albicans is an important factor in the development of DRS. Hyphae seem to facilitate the rise of C. albicans counts and be related to the inflammatory response of the tissues. Lactobacillus seems to play an important role in the presence of DRS, as well. In agreement with many other studies, the results of this study confirm the importance of denture age and continuous denture wearing in the development of DRS.

Topics: Aged; Benzenesulfonates; Candida albicans; Candidiasis, Oral; Colony Count, Microbial; Dental Plaque Index; Denture, Complete; Female; Fluorescent Dyes; Humans; Hyphae; Lactobacillus; Male; Middle Aged; Oral Hygiene; Palate; Saliva; Stomatitis, Denture; Time Factors

Calcofluor white (CW) is a water-soluble, colorless dye that selectively binds to beta 1-4 polysaccharides (cellulose) in fungal cell walls. The planar, trans-form of the molecule fluoresces light blue when exposed to UV light (345 to 365 nm). Recuts of all biopsy specimens, accessioned over a 6-month period, that had been stained with Grocott's methenamine silver (GMS) were stained with CW and examined. Of the 34 cases from which specimens were reviewed, 17 had specimens that were positive for the presence of fungal organisms as indicated by both GMS and CW staining. Specimens from all of the 17 remaining cases were negative for the presence of fungal organisms, as staining with both GMS and CW indicated. Similar results were obtained with direct fungal smears. The CW technique has significant advantages over traditional methods, including rapidity, cost, sensitivity, simplicity, and absence of interference with permanent fungal stains.

Topics: Benzenesulfonates; Candidiasis, Oral; Diagnosis, Differential; Fluorescent Dyes; Histological Techniques; Humans; Staining and Labeling