c-peptide and Pulmonary-Fibrosis

c-peptide has been researched along with Pulmonary-Fibrosis* in 1 studies

Other Studies

1 other study(ies) available for c-peptide and Pulmonary-Fibrosis

ArticleYear
C-peptide attenuates hyperglycemia-induced pulmonary fibrosis by inhibiting transglutaminase 2.
    Journal of molecular endocrinology, 2022, 04-29, Volume: 68, Issue:4

    Proinsulin C-peptide has a protective effect against diabetic complications; however, its role in hyperglycemia-induced pulmonary fibrosis is unknown. In this study, we investigated the inhibitory effect of C-peptide on hyperglycemia-induced pulmonary fibrosis and the molecular mechanism of C-peptide action in the lungs of diabetic mice and in human pulmonary microvascular endothelial cells (HPMVECs). We found that, in the lungs of diabetic mice, C-peptide supplementation using osmotic pumps attenuated hyperglycemia-induced pulmonary fibrosis and expression of fibrosis-related proteins. In HPMVECs, C-peptide inhibited vascular endothelial growth factor-induced adherens junction disruption and endothelial cell permeability by inhibiting reactive oxygen species generation and transglutaminase (TGase) activation. In the lungs, C-peptide supplementation suppressed hyperglycemia-induced reactive oxygen species generation, TGase activation, and microvascular leakage. C-peptide inhibited hyperglycemia-induced inflammation and apoptosis, which are involved in the pathological process of pulmonary fibrosis. We also demonstrated the role of TGase2 in hyperglycemia-induced vascular leakage, inflammation, apoptosis, and pulmonary fibrosis in the lungs of diabetic TGase2-null (Tgm2-/-) mice. Furthermore, we demonstrated a long-term inhibitory effect of systemic delivery of C-peptide using K9-C-peptide hydrogels on hyperglycemia-induced fibrosis in diabetic lungs. Overall, our findings suggest that C-peptide alleviates hyperglycemia-induced pulmonary fibrosis by inhibiting TGase2-mediated microvascular leakage, inflammation, and apoptosis in diabetes.

    Topics: Animals; C-Peptide; Diabetes Mellitus, Experimental; Endothelial Cells; Hyperglycemia; Inflammation; Mice; Mice, Inbred C57BL; Protein Glutamine gamma Glutamyltransferase 2; Pulmonary Fibrosis; Reactive Oxygen Species; Transglutaminases; Vascular Endothelial Growth Factor A

2022