bromochloroacetic-acid and Uterine-Diseases

Abnormal endometrial repair after injury results in the formation of intrauterine adhesions (IUA) and a thin endometrium, which are key causes for implantation failure and infertility. Stem cell transplantation offers a potential alternative for some cases of severe Asherman's syndrome that cannot be treated with surgery or hormonal therapy. Umbilical cord-derived mesenchymal stem cells (UCMSCs) have been reported to repair the damaged endometrium. However, there is no report on the effects of UCMSCs previously seeded on human acellular amniotic matrix (AAM) on endometrial injury.. Absolute ethanol was injected into rat uteri to damage the endometrium. UCMSCs previously seeded on AAM were surgically transplanted. Using a variety of methods, the treatment response was assessed by endometrial thickness, endometrial biomarker expression, endometrial receptivity, cell proliferation, and inflammatory factors.. Endometrial thickness was markedly improved after UCMSC-AAM transplantation. The expression of endometrial biomarkers, namely, vimentin, cytokeratin, and integrin. UCMSC transplantation using AAM as the carrier can be applied to treat endometrial injury in rats. The successful preparation of lyophilized AAM provides the possibility of secondary infectious disease screening and amniotic matrix quality detection, followed by retrospective analysis. The UCMSC-AAM complex may promote the better application of UCMSCs on the treatment of injured endometrium.

Topics: Amnion; Animals; Biomarkers; Cell Transplantation; Disease Models, Animal; Endometrium; Female; Humans; Inflammation; Integrins; Keratins; Mesenchymal Stem Cells; Placenta; Pregnancy; Rats; Rats, Sprague-Dawley; Regeneration; Retrospective Studies; Stem Cell Transplantation; Tissue Adhesions; Umbilical Cord; Uterine Diseases; Uterus; Vimentin

The present study aimed to investigate the effects of menstrual blood‑derived stem cells (MenSCs) on endometrial injury repair. MenSCs were isolated from human menstrual blood and were cultured in vitro. Flow cytometric analysis of cells in the third generation demonstrated that MenSCs exhibited higher expression levels of cluster of differentiation (CD)90 and lower expression levels of CD146, which suggested that the MenSCs were cultured successfully. A mechanical damage model of unilateral (right) endometrium was established in BALB/c nude mice, which were divided into four groups, Normal, negative control (NC), Model and MenSC. MenSCs transfected with adenovirus‑enhanced green fluorescent protein were transplanted into the right uterine cavity of mice in the MenSC and NC groups. The protein expression levels of keratin, vimentin, and vascular endothelial growth factor (VEGF) and the average endometrial thickness were measured by immunohistochemistry; the average optical density of vimentin, VEGF and keratin in the MenSC‑treated group was significantly higher compared with the untreated Model group. Fertility tests were performed to determine the pregnancy rate of each group; following endometrial damage in BALB/c nude mice, endometrial thickness was decreased in the Model group, whereas model mice treated with MenSC exhibited increased endometrial thickness and increased the pregnancy rates. Therefore, MenSCs may promote the repair of endometrial lesions in mice by promoting the expression of vimentin, VEGF and keratin.

Topics: Adult; Animals; CD146 Antigen; Cell Differentiation; Cell Proliferation; Cells, Cultured; Endometrium; Female; Humans; Keratins; Mesenchymal Stem Cells; Mice, Inbred BALB C; Mice, Nude; Pregnancy; Pregnancy Rate; Uterine Diseases; Vascular Endothelial Growth Factor A; Vimentin; Young Adult

Topics: Adult; Biomarkers; Endometrium; Female; Humans; Keratins; Metaplasia; Uterine Diseases

We measured serum cytokeratin fragment 21-1 (CYFRA 21-1) levels by a solid-phase immunoradiometric assay in 102 healthy Japanese women, and set the reference value at 1.9 ng/ml (mean +2 SD of the serum levels based on a linear distribution). Pretreatment serum CYFRA 21-1 levels were also analyzed in 235 women with benign (n = 94) or malignant (n = 141) gynecologic disease, and were compared with the serum levels of CA 125 and SCC. The respective positivity rates for CYFRA 21-1 and CA 125 were 64.0 and 77.2% in ovarian malignancy, while they were 4.2 and 30.8% in benign ovarian masses. CYFRA 21-1 had an accuracy of 61.3% in diagnosing ovarian malignancy, which was higher than that of CA 125 (53.4%). The positive predictive value of CYFRA 21-1 for ovarian malignancy reached 94.1%, which was significantly (p < 0.005) higher than that of CA 125 (68.8%). These findings indicate the potential usefulness of CYFRA 21-1 as a tumor marker for ovarian malignancy. In addition, the positivity rates fo CYFRA 21-1 in cervical cancer (51.2%) and endometrial cancer (52.2%) were also similar to the respective rates for SCC and CA 125, which suggests that CYFRA 21-1 seems to be a general tumor marker for gynecologic malignancy.

Topics: Adolescent; Adult; Biomarkers, Tumor; CA-125 Antigen; Cysts; Endometrial Neoplasms; Female; Genital Neoplasms, Female; Humans; Immunoradiometric Assay; Keratins; Middle Aged; Ovarian Diseases; Ovarian Neoplasms; Reference Values; Uterine Cervical Neoplasms; Uterine Diseases; Uterine Neoplasms

Thirty endometrial biopsies were cultured in order to separate stromal and epithelial cells. Using epidermal growth factor (EGF), cortisol, cholera toxin, insulin with 5% horse serum for epithelial cells or a medium with 20% fetal calf serum for stromal cells, we could specifically enrich endometrial culture in epithelial or stromal cells and culture them for 1 or 2 months. These cultures retained the phenotypic characteristics of epithelial (cytokeratins, mucin HMFG 1) and stromal (vimentin, smooth muscle actin, desmin) lineage by immunostaining analysis. Epithelial and stromal cultures from one individual were respectively immortalized by the SV 40 large T antigen. The immortalized cell lines kept the phenotype of the normal cells from which they derived.

Topics: Actins; Adolescent; Adult; Antigens, Polyomavirus Transforming; Biomarkers; Cell Division; Cell Line, Transformed; Cell Separation; Cell Transformation, Viral; Cells, Cultured; Desmin; Endometrium; Epithelial Cells; Epithelium; Female; Growth Substances; Humans; Immunohistochemistry; Keratins; Menstrual Cycle; Middle Aged; Mucins; Phenotype; Recombinant Proteins; Simian virus 40; Transfection; Uterine Diseases; Vimentin

The purpose of this study was to determine the nature of the argyrophilic cells in the ectocervix and to analyze the different morphologic varieties of argyrophil cell-containing ectocervical epithelia, notably to reappraise the degree of analogy with transitional epithelium. A systematic study of 39 ectocervices was carried out using histochemical and immunohistochemical techniques. Immunodetection of cytokeratins was used to specify the differentiation of the ectocervical linings. Argyrophilic cells were detected in 43% of our specimens. They have been found in several varieties of ectocervical lining: normal-appearing or hyperkeratotic squamous epithelium, "transitional-like" epithelium extending onto the portio, and immature squamous metaplasia from the transformation zone. The term "transitional-like" refers to a stratified nonsquamous epithelium that differs from true urothelium by the absence of superficial differentiation leading to the layer of "umbrella" cells. Two main types of argyrophilic cells have been delineated: serotonin cells and Merkel-type cells. The nature of the argyrophilic cells was dependent on the type of epithelium: Serotonin cells were essentially associated with "transitional-like" epithelium and Merkel-type cells with squamous epithelium. The latter cells were particularly frequent in hyperkeratotic squamous epithelium from uterine prolapse, reinforcing the homology with epidermis.

Topics: Adult; Aged; Cervix Uteri; Chromogranin A; Chromogranins; Epithelium; Female; Histocytochemistry; Humans; Immunoenzyme Techniques; Keratins; Middle Aged; Serotonin; Uterine Diseases; Uterine Neoplasms

Topics: Animals; Cell Transformation, Neoplastic; Endometrium; Epithelium; Female; Hyperplasia; Hypertrophy; Intrauterine Devices; Keratins; Metaplasia; Ovarian Diseases; Ovary; Polyps; Rats; Time Factors; Uterine Diseases; Uterus