bromochloroacetic-acid and Stomach-Ulcer

Esophagogastric ulcer is an independent disease in swine that is characterized by ulcerous autodigestion of the cutaneous mucosa, which does not exhibit a tendency to recover, but, on the contrary, a tendency toward severe hemorrhaging, with a predominantly lethal outcome. Since it develops in the part of the stomach that is morphologically and functionally different from other glandular mucosa, it was questioned earlier whether it could be a peptic ulcer based on its nature. Spontaneous ulcers, usually of the stomach, commonly occur in many domestic animals. Some of these lesions are chronic and they may occur in either the glandular or squamous-lined regions of the stomach. As with the human disease, the pathogenesis in domestic animals is multifactorial, poorly understood, and variable between and within species. Environmental stress and dietary factors are very important in the ulcer disease in swine. It has been shown that the Helicobacter spp. is strongly associated with naturally occurring ulcer and preulcer lesions of the pars esophagea in swine, which raises the possibility that Helicobacter spp. is an important factor in the pathogenesis of these lesions. The dynamics of the development of esophagogastric ulcers imply hyperplastic lesions (parakeratosis and hyperkeratosis), keratolysis, erosions, peptic necrosis, and the development of ulcers with all the characteristics of peptic ulcerations in other localities. In addition, K6 is expressed in association with the mucosal changes. The pattern of the intermediate filaments of keratin suggests that epithelial proliferation, which leads to visible hyperkeratosis, constitutes the essence of gastric ulcers in swine.

Topics: Animals; Cell Proliferation; Chronic Disease; Diagnosis, Differential; Diet; Esophageal Diseases; Esophagogastric Junction; Helicobacter Infections; Humans; Intermediate Filaments; Keratins; Stomach; Stomach Ulcer; Swine

Topics: Aminocaproic Acid; Animals; Cytoskeleton; Endoscopy; Female; Fluorescent Dyes; Gastric Juice; Hair; Humans; Keratins; Mice; Mice, Inbred BALB C; Regeneration; Spectroscopy, Fourier Transform Infrared; Stomach Ulcer; Ulcer; Viscosity; Wound Healing

An 81-year-old man presented with epigastric pain and weight loss for one month. He had a past history of pulmonary tuberculosis, 10 years ago. We performed a gastroscopy, which showed a linear depressed whitish gastric ulcer scar (0.8 cm in length) in the posterior wall of the prepyloric antrum. The result of biopsy was reported as squamous epithelium. Immunohistochemical staining using an antibody to high molecular weight cytokeratin (HMC) revealed positive staining in the squamous epithelium. Two years later, the lesion was followed up. The lesion remained at same site endoscopically, but no squamous epithelium could be seen microscopically.

Topics: Abdominal Pain; Aged, 80 and over; Biopsy; Epithelium; Humans; Immunohistochemistry; Keratins; Male; Metaplasia; Molecular Weight; Stomach Ulcer; Treatment Outcome; Ulcer

Bone marrow contains hematopoietic stem cells, nonhematopoietic mesenchymal stem cells, and several precursor cells for osteoblasts, chondrocytes, adipocytes, myocytes, hepatocytes, and even neural cells. Research findings indicate that multipotent stem cells in the adult body may be used to recover the lost functions of damaged tissues. This study examined the involvement of bone marrow-derived cells in the regeneration of the stomach after experimental gastric ulcers were produced in rats.. We transplanted the bone marrow of transgenic rats that expressed green fluorescence protein (GFP) throughout the body. Twenty-one days after the bone marrow transplantation (BMT), gastric ulceration was induced, using absolute ethanol. Control animals received saline. After various observation periods, rats harboring GFP-positive bone marrow-derived cells were killed, and the tissues were removed and processed to prepare paraffin-embedded sections. Cells expressing GFP were identified by conventional immunohistochemistry, using anti-GFP antibody. To identify whether cells expressing GFP were epithelial cells or interstitial cells such as fibroblasts, serial sections were examined with anti-cytokeratin antibody or anti-vimentin antibody, respectively. Furthermore, to confirm that cells expressing GFP were epithelial cells or interstitial cells, we used double-staining analysis with anti-GFP antibody or anti-cytokeratin antibody, respectively.. GFP-positive, bone marrow-derived cells were found in the cytokeratin-positive gastrointestinal epithelium, as well as among vimentin-positive interstitial cells. Interestingly, the proportions of GFP-positive, cytokeratin-positive epithelial cells and vimentin-positive interstitial cells were significantly greater in the ethanol-treated damaged stomachs than in the saline-treated controls.. The present study clearly demonstrates that bone marrow-derived cells are involved in the regeneration of the stomach after ethanol-induced ulcers in rats.

Topics: Animals; Bone Marrow Cells; Bone Marrow Transplantation; Cell Count; Central Nervous System Depressants; Disease Models, Animal; Ethanol; Green Fluorescent Proteins; Immunohistochemistry; Intestinal Mucosa; Keratins; Rats; Rats, Sprague-Dawley; Regeneration; Severity of Illness Index; Stomach; Stomach Ulcer; Treatment Outcome

Topics: Biomarkers, Tumor; Biopsy; Cell Transformation, Neoplastic; Gastric Mucosa; Gastritis; Gastroscopy; Humans; Keratins; Metaplasia; Polyps; Precancerous Conditions; Stomach Neoplasms; Stomach Ulcer

The finding of cytokeratin positivity in non-epithelial cells in 9/15 cases of performing or penetrating gastric ulcer is reported. Two different monoclonal anti-cytokeratin antibodies were used and both produced a strong positivity in spindle and polyglonal cells in the gastric wall. These cells were often distributed near the peritoneal surface, but they were also found in central parts of the gastric wall. The cytokeratin-positive cells had no connection with the gastric mucosa, lacked epithelial features in routinely stained sections and were not positively stained by antibodies to other epithelial markers (EMA and Ber-Ep 4). Many of the cytokeratin-positive cells were also positive for vimentin. There was no evidence of malignancy in any of the cases, but cytokeratin-positive cells like those in the present study may be erroneously interpreted as infiltrating carcinoma. The true nature of the cytokeratin-positive cells was not revealed in the present study. It is concluded that cytokeratin positivity must be evaluated with care and that it is valuable to add antibodies other than anti-cytokeratins for the recognition of epithelial cell differentiation.

Topics: Antibodies, Monoclonal; Cell Transformation, Neoplastic; Gastric Mucosa; Humans; Immunohistochemistry; Keratins; Stomach; Stomach Neoplasms; Stomach Ulcer; Vimentin

Topics: Animals; Brazil; Female; Gastric Mucosa; Keratins; Male; Stomach Ulcer; Swine; Swine Diseases