bromochloroacetic-acid and Plasmacytoma

Extramedullary plasmacytoma of the pancreas is a rare entity. Although this condition is uncommon, it should be considered in the differential diagnosis of solid mass in the pancreas, especially in patients with underlying multiple myeloma. We report a case of pancreatic plasmacytoma in a 56-year-old woman with newly diagnosed multiple myeloma. We highlight this rare manifestation of multiple myeloma among other better recognised presentations.

Topics: Diagnosis, Differential; Female; Humans; Immunohistochemistry; Keratins; Middle Aged; Multiple Myeloma; Pancreas; Pancreatic Neoplasms; Plasmacytoma; Syndecan-1

Topics: Breast Neoplasms; Cadherins; Carcinoma, Lobular; Catenins; Delta Catenin; Diagnosis, Differential; Female; Humans; Keratins; Lymphoma; Mastitis; Plasmacytoma

Plasmacytomas are localized neoplastic proliferations of monoclonal plasma cells. When multifocal, the process is referred to as multiple myeloma. These lesions exhibit a pattern of antigen expression and cytomorphology that usually leads to a ready diagnosis. However, potentially troublesome variations in immunophenotype occur. We describe a case of a plasmacytoma from a patient who presented with sudden onset of pain and a lytic lesion of the left proximal humerus. Hematoxylin and eosin-stained sections showed a lymphoproliferative lesion composed of large lymphoid cells, some with plasmacytoid and immunoblastic features. The lesion also showed significant mitotic activity. Immunohistochemical staining was positive for CD45 (LCA), CD56 (N-CAM), CD43 (MT1), and cytokeratin CAM5.2. There was also clonal staining for lambda light chains. In addition, flow cytometric analysis showed positivity for myeloid markers such as CD13, CD33, CD38, and CD138. Significant negative markers include CD20 (L26), CD45RO (UCHL-1), and CD79alpha. The unusual phenotypic features of this plasmacytoma illustrate potential diagnostic pitfalls. It is important to fully study such lesions to correctly classify them, because this has significant impact on prognosis and management.

Topics: Antigens, Differentiation, T-Lymphocyte; Antigens, Neoplasm; Bone Neoplasms; Humans; Humerus; Immunophenotyping; Keratins; Plasmacytoma

In this study the recently developed keratin 19 antibody RCK108 is biochemically and immunohistochemically characterized. Its applicability as a keratin marker in routinely processed histological tissue specimens is assessed.. The keratin 19 antibody RCK108 antibody was tested on normal and malignant routinely formalin-fixed, paraffin-embedded tissue specimens. It stains most, although not all, glandular epithelia and showed (focal) reactivity in the basal cell compartment of stratified epithelia. It was found to react with most epithelial tumours, including adenocarcinomas, squamous cell carcinomas and endocrine tumours of various origins.. Its reproducible and highly sensitive staining characteristics make RCK108 a useful antibody to be applied as a broad epithelial marker for carcinoma detection in routinely processed paraffin sections. As such, RCK108 is a specific reagent for practically all epithelial tumours. A few types of epithelial malignancies, known not to contain keratin 19, were negative for RCK108. Therefore the antibody is also useful in some narrow differential diagnostic considerations such as cholangiocellular carcinoma (RCK108 positive) vs. hepatocellular carcinoma (RCK108 negative). Another important feature of this antibody is that it shows very little reactivity in mesenchymal tissues, or mesenchymally derived tumours, as is frequently described for other keratin antibodies. A few leiomyosarcomas showed sporadic reactivity.

Topics: Animals; Antibodies, Monoclonal; Antigen-Antibody Reactions; Biomarkers, Tumor; Cell Line; Epithelial Cells; Humans; Immunohistochemistry; Keratins; Mice; Mice, Inbred BALB C; Neoplasms; Plasmacytoma; Reproducibility of Results; Sensitivity and Specificity

A case of primary gastric plasmacytoma expressing various surface and cytoplasmic antigens is reported. With the use of formalin-fixed and deparaffinized sections, 13 different antibodies were applied. Neoplastic plasma cells revealed monoclonal IgG and kappa light chain in the cytoplasm, and expressed epithelial membrane antigen, Ki 67 antigen, cytokeratin, CD 22 antigen, interleukin-2 receptor antigen, leukocyte common antigen and Ki-1 (CD 30) antigen. However, tumor cells were devoid of HLA-DR antigen. These data suggest that the neoplastic plasma cells are at the plasmoblastic stage of maturation and express various surface and cytoplasmic phenotypes.

Topics: Aged; Aged, 80 and over; Antigens, CD; Antigens, Differentiation; Antigens, Differentiation, B-Lymphocyte; Antigens, Neoplasm; Antigens, Surface; Cell Adhesion Molecules; Female; Humans; Immunohistochemistry; Keratins; Ki-1 Antigen; Ki-67 Antigen; Lectins; Leukocyte Common Antigens; Membrane Glycoproteins; Mucin-1; Plasmacytoma; Receptors, Interleukin-2; Sialic Acid Binding Ig-like Lectin 2; Stomach Neoplasms

Topics: Adenocarcinoma; Colonic Neoplasms; Humans; Immunohistochemistry; Keratins; Lymph Nodes; Plasma Cells; Plasmacytoma

Fourteen plasma cell tumours, including examples of solitary plasmacytoma and multiple myeloma, were studied with a panel of antibodies reactive in formalin-fixed, paraffin wax-embedded tissue. Each case showed immunoglobulin light chain restriction. Five tumours were reactive with antibodies to cytokeratin. Of these five cases, four were negative with antibodies to leucocyte common antigen and only one was weakly positive. Anti-cytokeratin reactivity by plasma cell tumours is more common than was originally anticipated and represents an important diagnostic pitfall.

Topics: Humans; Immunohistochemistry; Keratins; Lymphoma; Multiple Myeloma; Plasmacytoma

Topics: Humans; Immunohistochemistry; Keratins; Plasmacytoma

Hybridomas producing mouse monoclonal antibodies to antigens of the human mammary carcinoma cell line, MCF-7, have been generated by electric field-mediated fusion at a frequency ten times higher than by polyethylene glycol. One of the monoclonal antibodies obtained recognizes a cytoskeletal structure restricted to epithelial cells and carcinomas with a distribution pattern resembling cytokeratin 19.

Topics: Animals; Antibodies, Monoclonal; Antigens, Neoplasm; Cell Fusion; Cell Line; Electricity; Female; Humans; Hybridomas; Keratins; Mice; Mice, Inbred BALB C; Plasmacytoma; Polyethylene Glycols; Spleen

A monoclonal antibody derived from a mouse immunized with bovine epidermal prekeratin has been characterized by its binding to cytoskeletal polypeptides separated by one- or two-dimensional gel electrophoresis and by immunofluorescence microscopy. This antibody (KG 8.13) binds to a determinant present in a large number of human cytokeratin polypeptides, notably some polypeptides (Nos. 1, 5, 6, 7, and 8) of the 'basic cytokeratin subfamily' defined by peptide mapping, as well as a few acidic cytokeratins such as the epidermis-specific cytokeratins Nos. 10 and 11 and the more widespread cytokeratin No. 18. This antibody reacts specifically with a wide variety of epithelial tissues and cultured epithelial cells, in agreement with previous findings that at least one polypeptide of the basic cytokeratin subfamily is present in all normal and neoplastic epithelial cells so far examined. The antibody also reacts with corresponding cytokeratin polypeptides in a broad range of species including man, cow, chick, and amphibia but shows only limited reactivity with only a few rodent cytokeratins. The value of this broad-range monoclonal antibody, which apparently recognizes a stable cytokeratin determinant ubiquitous in human epithelia, for the immunohistochemical identification of epithelia and carcinomas is discussed.

Topics: Animals; Antibodies, Monoclonal; Cattle; Cross Reactions; Epithelial Cells; Epitopes; Female; Fluorescent Antibody Technique; Humans; Hybridomas; Keratins; Mice; Mice, Inbred Strains; Plasmacytoma; Radioimmunoassay; Skin; Species Specificity; Tissue Distribution

Topics: Alpha-Globulins; Arthritis, Rheumatoid; Beta-Globulins; Blood Protein Electrophoresis; Brachial Plexus Neuritis; Cerebrovascular Disorders; Electrophoresis, Disc; gamma-Globulins; Gold; Gout; Humans; Injections, Intramuscular; Injections, Intravenous; Keratins; Plasmacytoma; Protein Binding; Serum Albumin; Thiosulfates