bromochloroacetic-acid and Palatal-Neoplasms

Topics: Adult; Diagnosis, Differential; Female; Follow-Up Studies; Glial Fibrillary Acidic Protein; Humans; Keratins; Myoepithelioma; Palatal Neoplasms; Plasma Cells; S100 Proteins; Vimentin

Assessing epithelial dysplasia to predict malignant transformation remains problematic in many tissues because grading systems are poorly structured and individual features poorly defined. Dysplasia grading is criticised for lack of reproducibility and poor predictive value. Grading systems for upper aerodigestive tract dysplasia have evolved over several decades and are not supported by good outcome experimental data..  This study analysed the individual features of dysplasia in 86 oral dysplastic lesions and determined the reproducibility of scoring for each, and correlated them with other features and clinical factors using complex clustering analyses.. A uniform pattern of dysplasia was found in 37 lesions, focal dysplasia in 36 and in 13 lesions dysplasia formed complex discontinuous patterns. There was wide variation in reproducibility of scoring of individual features and many, including thickness, some types of rete morphology, basaloid cell anisonucleosis, basal dyscohesion, and dyskeratosis as deep single cells correlated with sub-sites. Rete morphology, type of keratinisation, hyperchromatism of the basaloid compartment, prickle cell anisonucleosis and extension down salivary ducts correlated with smoking. Conventional grading and oral intraepithelial neoplasia (OIN) grading by 'thirds affected' showed strong correlation overall but scores obtained with the OIN system tended to a higher grade at all sites except soft palate/fauces. There was poor correlation between the systems for moderate dysplasia and also severe dysplasia at some sites. Individual features could not be shown to cluster to form distinct patterns of dysplasia.. These variations may account in part for the lack of reproducibility and poor predictive value of the grading systems in current use and could inform the design of future grading systems.

Topics: Carcinoma in Situ; Cell Adhesion; Cell Nucleus; Cell Transformation, Neoplastic; Chromatin; Epithelial Cells; Epithelium; Female; Humans; Keratins; Leukoplakia, Oral; Lip Neoplasms; Male; Mitosis; Mouth Floor; Mouth Mucosa; Mouth Neoplasms; Neoplasm Grading; Palatal Neoplasms; Palate, Soft; Precancerous Conditions; Reproducibility of Results; Salivary Ducts; Tongue Neoplasms

The aim of this study was to present a rare neoplasm, Primary myoepithelial carcinoma arising from the palate, and to review its diagnostic criteria, pathologic and clinical characteristics, treatment options and prognosis.. Myoepitheliomas are tumors arising from myoepithelial cells mainly or exclusively. Myoepitheliomas mostly occur in salivary glands, as well as in breast, skin, and lung. Case of myoepitheliomas in palate has rarely been reported. Myoepithelial carcinoma is malignant counterpart of myoepitheliomas. Adenomyoepithelioma is also a different disease from myoepitheliaomas. Immunohistochemically, tumor cells of myoepithelial carcinoma express not only epithelial markers such as cytokeratin, epithelial membrane antigen (EMA), but also markers of smooth muscle origin such as calponin. The immunohistochemical criteria of myoepithelial differentiation are double positive for both cytokeratins and one or more myoepithelial immunomarkers (i.e., S-100 protein, calponin, p63, GFAP, maspin, and actins). Myoepithelial carcinomas of salivary and breast demonstrate copy number gains and gene deletion. The overall prognosis of myoepithelial carcinoma is poor. There is rarely recurrence or metastasis in benign myoepithelial tumors. Complete excision with tumor-free margin is always the preferred treatment, while local radiation therapy and chemotherapy are suggestive treatment options. Here, a rare case of myoepithelial carcinoma arising from the palate has been described and discussed for the treatment and outcome. Pathological and clinical characters of myoepitheliomas are also compared and discussed.. The case report serves to increase awareness and improve the index of diagnosis and treatment of myoepitheliomas.

Topics: Aged; Biomarkers, Tumor; Biopsy; Calcium-Binding Proteins; Calponins; Combined Modality Therapy; Diagnosis, Differential; Follow-Up Studies; Humans; Immunohistochemistry; Keratins; Male; Microfilament Proteins; Muscle Proteins; Myoepithelioma; Palatal Neoplasms; S100 Proteins

Squamous cell carcinoma ex-pleomorphic adenoma (CXAP) is a malignant and rare mixed tumor. We report a new case.. A seventy-year-old woman consulted for a mass in the left hemi-face having evolved over the last 20years. The physical examination revealed a hard and large tumor invading all the palate. Computed tomography revealed a heterogeneous 8.5cm long maxillary mass. The diagnosis of CXAP was made on a biopsy. A histological study confirmed the diagnosis after surgical resection of the tumor, specifying its noninvasive character.. CXAP is generally located in the parotid gland; it is very rarely located in the palate. The degenerated epithelial component generally corresponds to an adenocarcinoma or an undifferentiated carcinoma; squamous-cell carcinoma is more rarely reported. The prognosis is excellent for the micro and noninvasive types. Surgery remains the treatment of choice.

Topics: Adenoma, Pleomorphic; Aged; Amyloid; Biopsy; Carcinoma, Squamous Cell; Cell Nucleus; Epithelial Cells; Female; Follow-Up Studies; Humans; Keratins; Neoplasms, Multiple Primary; Neprilysin; Palatal Neoplasms; Tomography, X-Ray Computed

This study compares synchronous oral squamous cell carcinomas (OSCCs) with single primary OSCCs to assess the histopathologic parameters with a known prognostic significance.. Twenty-eight cases of synchronous OSCCs and a control group of single primary OSCCs were compared for 15 histologic prognostic variables.. Results showed significantly less amount of abnormal mitoses (synchronous-1: P = .002; synchronous-2: P = .006) and tumor-induced stroma (synchronous-1: P = .011; synchronous-2: P = .001) in synchronous OSCCs than in single primary OSCCs. Depth of invasion was considerably lower in synchronous OSCCs than in single primary OSCCs (synchronous-1: P = .007; synchronous-2: P = .002). Lymph node metastasis (synchronous-1: P = .051; synchronous-2: P = .051) was found to be rare in synchronous OSCCs compared with single primary OSCCs.. Synchronous OSCCs show less aggressive histopathologic features than single primary OSCCs.

Topics: Aged; Carcinoma, Squamous Cell; Case-Control Studies; Cell Differentiation; Female; Gingival Neoplasms; Humans; Keratins; Lip Neoplasms; Lymphatic Metastasis; Male; Middle Aged; Mitosis; Mouth Mucosa; Mouth Neoplasms; Neoplasm Invasiveness; Neoplasms, Multiple Primary; Palatal Neoplasms; Tongue Neoplasms

Myoepithelioma is a rare benign salivary gland tumour, localized most frequently in the parotid and in minor intraoral salivary glands. There have been only four cases of myoepithelioma in children and adolescents reported in the English-language literature, all of them involving the plasmacytoid variant.. A 13-year-old boy, complained of a painless nodule of the palate. Incisional biopsy was performed and revealed large plasmacytoid cells with a round and eccentric nuclei. The diagnosis was consistent with myoepithelioma, plasmocytoid variant. Tumour cells were positive for cytokeratins, vimentin and S-100 protein. Surgical resection was performed and no evidences of tumour recurrence were observed after 6 years of the treatment.. Myoepithelioma is a very rare tumour in children and apparently presents a good prognosis, similar to occur in adult patients.

Topics: Adolescent; Biomarkers, Tumor; Cell Nucleus; Follow-Up Studies; Humans; Keratins; Male; Myoepithelioma; Palatal Neoplasms; S100 Proteins; Vimentin

Topics: Adenocarcinoma, Clear Cell; Aged; Diagnosis, Differential; Female; Humans; Keratins; Male; Mucin-1; Palatal Neoplasms; Palate, Hard; Tongue Neoplasms

Sialadenoma papilliferum (SP) is a rare benign tumour of salivary gland origin, which has been included among the ductal papillomas in the latest classification of tumours by the World Health Organisation. Two SP from the minor salivary gland of the palate of middle age patients were presented and studied by immunohistochemical. Our results showed presence of cytokeratins (CKs) 13, 14, 7, 8, 19 and absence of vimentin and smooth muscle actin. This immunoprofile is similar to the excretory duct of salivary gland.

Topics: Adenoma; Female; Humans; Immunohistochemistry; Keratins; Male; Middle Aged; Palatal Neoplasms; Palate, Hard; Palate, Soft; Salivary Gland Neoplasms; Salivary Glands, Minor

Topics: Aged; Aneuploidy; Biopsy; Carcinoma; Diagnosis, Differential; Diploidy; Female; Flow Cytometry; Humans; Keratins; Male; Middle Aged; Palatal Neoplasms; S100 Proteins; Salivary Ducts; Salivary Gland Neoplasms; Salivary Glands, Minor

Salivary duct carcinoma is an uncommon malignant salivary gland tumor that occurs predominantly in the parotid gland. Oral involvement is extremely rare, with few cases having been reported in the literature. The tumor is characterized by an aggressive behavior and has a poor prognosis. We describe a case of salivary duct carcinoma arising in the hard palate of a 63-year-old man. Immunohistochemical analysis revealed that tumor cells tested positive for cytokeratin, epithelial membrane antigen, proliferating cell nuclear antigen, Ki67, p53, laminin, and collagen IV. Despite radical surgical resection, bilateral neck dissection, and postoperative radiotherapy, liver metastases developed, and the patient subsequently died of his disease.

Topics: Carcinoma; Collagen; Fatal Outcome; Humans; Immunohistochemistry; Keratins; Ki-67 Antigen; Laminin; Liver Neoplasms; Male; Middle Aged; Mucin-1; Palatal Neoplasms; Prognosis; Proliferating Cell Nuclear Antigen; Radiotherapy, Adjuvant; Salivary Ducts; Salivary Gland Neoplasms; Salivary Glands, Minor; Tumor Suppressor Protein p53

A 53-year-old Japanese woman presented with a mass of the hard palate that was histologically diagnosed as myoepithelioma. By CT, the well-demarcated ovoid isodense mass showed faint contrast enhancement. By MR imaging, the mass was homogeneous and isointense on T1-weighted images with homogeneous enhancement after administration of contrast material and markedly hyperintense on T2-weighted images. Myoepithelioma should be kept in mind as differential diagnosis of hard palatine tumors.

Topics: Actins; Contrast Media; Diagnosis, Differential; Diagnostic Imaging; Female; Gadolinium DTPA; Humans; Image Enhancement; Keratins; Magnetic Resonance Imaging; Middle Aged; Myoepithelioma; Palatal Neoplasms; Radiographic Image Enhancement; S100 Proteins; Tomography, X-Ray Computed; Vimentin

This case report describes a unique palatal tumor with features of a dermal neoplasm. Microscopically, the lesion appeared similar to a trichoepithelioma and trichoadenoma.. Light microscopic and immunohistochemical studies were performed to arrive at the final diagnosis.. The lesion arose from the surface epithelium and had features of a dermal tumor.. The case report describes a unique benign palatal neoplasm.

Topics: Adenoma, Pleomorphic; Adult; Diagnosis, Differential; Epithelial Cells; Epithelium; Female; Humans; Hyalin; Immunohistochemistry; Keratins; Keratosis; Mouth Mucosa; Neoplasms, Basal Cell; Palatal Neoplasms; Palate, Soft; Skin

The first case report of a merkel cell carcinoma arising from the palatal mucosa in a young adult is presented. The histopathological similarities of this tumour in skin and oral mucosa are also discussed. The patient was a 14-year-old female with a non-symptomatic painful swelling in the left molar region of the maxilla. Under the diagnosis of a malignant tumour, a partial maxillary resection was performed, but there was a recurrence, and finally the patient died of cerebral metastasis. The tumor was composed mainly of uniform small cells. Immunohistologically, a large number of the cells were reactive to neuron specific enolase (NSE) and cytokeratin CK19, and some of the cells were positive to CK8, CK13, CK20, PGP9.5 and CEA focally and slightly. Pseudo-rosette formation and squamous differentiation were frequently detected. The ultrastructure of the tumour cells showed abundant Golgi bodies associated with neurosecretory granules. We conclude that it is the first case of a Merkel cell tumour arising from palatal mucosa and invading underlying bone with reactive hyperplasia. These findings closely resemble those of the same tumour occurring in the skin

Topics: Adolescent; Carcinoma, Merkel Cell; Female; Humans; Immunohistochemistry; Keratins; Microscopy, Electron; Palatal Neoplasms; Phosphopyruvate Hydratase

Topics: Adenoma; Adult; Cell Nucleus; Epithelial Cells; Female; Follow-Up Studies; Humans; Keratins; Oral Ulcer; Palatal Neoplasms

The definition of plasmacytoid myoepithelioma, a neoplasm exhibiting myoepithelial differentiation, has been recently questioned. To better understand the histogenesis of this neoplasm, we searched for myoepithelial markers in histologic sections of plasmacytoid myoepithelioma and in a cell line (M1) derived from this same neoplasm.. Expression of vimentin, pan-keratin (AE-3) and smooth-muscle actin was studied by immunohistochemistry in paraffin-embedded tissue and by immunofluorescence in M1 cells.. Plasmacytoid myoepithelioma tumor sections showed vimentin and AE-3 reactivity, but evidence of smooth-muscle actin was not seen. The cell line derived from this tumor also produced vimentin and cytokeratin. In addition, all cultured cells expressed smooth-muscle actin.. We demonstrated that cells derived from a case of plasmacytoid myoepithelioma appear to show full myoepithelial differentiation in vitro. Thus, they are myoepithelial-like cells in nature. The lack of myogenous differentiation in vivo could be due to an inhibitory process mediated by the extracellular matrix.

Topics: 3,3'-Diaminobenzidine; Actins; Antibodies, Monoclonal; Biomarkers, Tumor; Cell Differentiation; Chromogenic Compounds; Coloring Agents; Extracellular Matrix; Female; Fluorescein; Fluorescent Antibody Technique, Direct; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Keratins; Microscopy, Fluorescence; Middle Aged; Myoepithelioma; Palatal Neoplasms; Paraffin Embedding; Plasma Cells; Tumor Cells, Cultured; Vimentin

Two cases of either peripheral odontogenic fibroma (POF) (WHO type) or peripheral ameloblastoma are reported. Their immunohistochemical characteristics were investigated in an attempt to clarify their histogenesis. The results showed that the epithelial component of this neoplasm tended to retain its distinct odontogenic character and expressed a keratin profile different from that of the overlying oral epithelium from which both cases most probably originated. The connective tissue element of these tumors was vimentin-positive and S-100 protein negative, confirming their mesodermal nature but precluding the possibility of ectomesenchymal derivation. No reactivity for desmin was noted.

Topics: Adult; Ameloblastoma; Desmin; Diagnosis, Differential; Female; Gingival Neoplasms; Humans; Immunohistochemistry; Keratins; Male; Maxilla; Odontogenic Tumors; Palatal Neoplasms; S100 Proteins; Vimentin

A case of epithelial-myoepithelial carcinoma (EMC) of the palate in a 72-year-old Japanese man is described. The patient had noticed swelling of the palate commencing about 20 years previously. Histologically, the tumor consisted of a proliferation of double-layered duct-like structures with two distinctive cell types. The inner layer was composed of eosinophilic epithelial cells, while the outer layer was composed of clear cells. Immunohistochemical analysis revealed that reaction products for total keratin were predominantly found in the cytoplasm of the inner epithelial cells, while those for S-100 protein and smooth muscle actin were observed only in the outer cells. Immunoreactive products for secretory component and lysozyme were found in some of the luminal contents and the inner cells of the tumor nests. These findings indicated this tumor to be an EMC of the palate, which had shown no aggressiveness over a twenty-year period prior to surgical excision.

Topics: Actins; Aged; Carcinoma; Cell Division; Cytoplasm; Epithelium; Humans; Immunohistochemistry; Keratins; Male; Muramidase; Palatal Neoplasms; S100 Proteins; Secretory Component

Topics: Adenocarcinoma; Adult; Cell Nucleus; Diagnosis, Differential; Humans; Hyalin; Keratins; Male; Middle Aged; Neoplasm Invasiveness; Palatal Neoplasms; S100 Proteins

Salivary duct carcinoma (SDC) is a distinctive salivary gland neoplasm morphologically characterized by intraductal and infiltrating components. Most tumors occur in the major salivary glands and demonstrate a propensity for invasive growth with early regional and distant metastases. Therefore, SDC is regarded as a high-grade malignancy in the current classification of salivary gland neoplasms.. In an effort to identify clinically relevant prognostic features, we studied the clinicopathologic and immunohistochemical findings in 15 SDC, with ultrastructural evaluation of three tumors.. Thirteen SDC occurred in the parotid gland, one in the Stensens duct, and one in the palate. Twelve patients were men (ratio of men to women = 4:1). Patients ranged in age from 39 to 81 years (mean = 59 years). Tumor size varied from 1.2 to 6.5 cm (mean = 3.1 cm). An intraductal component was identified in 10 of 14 primary SDC that made up 10% to 95% of the tumor. In three SDC a preexisting pleomorphic adenoma was identified. Immunohistochemical and electron microscopic evaluation showed the SDC to be composed entirely of ductal cells, and one tumor exhibited features of striated duct differentiation.. SDC show a broader clinicopathologic spectrum than previously described. The tumor may arise in a pleomorphic adenoma. The proportion of intraductal and extraductal growth is variable and of prognostic significance. Although the majority of SDC behave in a high-grade fashion, those with a predominant intraductal component of greater than 90% (PID-SDC) and minimally invasive (< 8 mm) SDC in pleomorphic adenoma appear to have a more favorable prognosis.

Topics: Adenoma, Pleomorphic; Adult; Aged; Aged, 80 and over; Carcinoma, Intraductal, Noninfiltrating; Cell Membrane; Cell Nucleus; Cytoplasm; Epithelium; Female; Humans; Hyalin; Hyperplasia; Keratins; Male; Middle Aged; Mitochondria; Neoplasm Invasiveness; Neoplasms, Multiple Primary; Palatal Neoplasms; Parotid Neoplasms

A survey of 4342 oral pathology reports accumulated over a five-year period was performed. Diagnoses were 1090 irritation fibromas and 116 giant cell fibromas. A statistical comparison was then made between the giant cell fibromas and the irritation fibromas to determine if there were any differences between these two lesions with respect to sex or race predilection, age distribution, or location in the oral cavity. Finally, various staining techniques were performed on the giant cell fibromas in an attempt to ascertain the origin of the giant cells present in these lesions. The results will be discussed in this paper.

Topics: Adolescent; Adult; Aged; Child; Child, Preschool; Collagen; Epithelium; Female; Fibroma; Giant Cells; Gingival Neoplasms; Humans; Hyperplasia; Immunoenzyme Techniques; Keratins; Male; Middle Aged; Mouth Neoplasms; Palatal Neoplasms; Tongue Neoplasms

Two cases of squamous odontogenic tumors are described in terms of histopathology and keratin immunohistochemistry. Histopathologically, the lesions were composed of squamous epithelial islands without peripheral columnar cells and well-differentiated stromal tissue. Immunohistochemical detection of keratin proteins was done with the use of polyclonal antikeratin antiserum (TK, detecting 41 to 65 kDa keratins) and monoclonal antibodies (KL1, 55 to 57 kDa; PKK1, 40, 45, and 52.5 kDa). Staining for PKK1-detectable keratin was absent in tumor epithelial cells and that with KL1 and TK immunoreagents was confined to squamous cells, being strong in the keratinized cells. In view of the results, the squamous odontogenic tumor appears to arise from the rests of Malassez in periodontal tissue rather than from oral squamous epithelium.

Topics: Adult; Female; Humans; Immunohistochemistry; Jaw Neoplasms; Keratins; Male; Middle Aged; Odontogenic Tumors; Palatal Neoplasms

A 24 year-old male with painful swelling over the right side of his palate for about two weeks was presented. An incisional biopsy was performed. In a routine hematoxylin and eosin examination by light microscopy, spindle and epithelioid cells with a bizarre appearance were discernible in the submucosal area. A pagetoid pattern was found in areas of the epithelium. Since this is not a remarkable finding, further examinations, such as the Trichrome-Masson and silver stain, immunohistochemistry using cytokeratin, vimentin, S-100, leukocyte common antigen, factor VIII, and alpha-1-antichymotrypsin detection kits, and electron microscopy were all carried out. According to the histological pattern of cells and the positive findings from the special stains, immunohistochemistry, and electron microscopy, a diagnosis of desmoplastic amelanotic melanoma was made. This variant of melanoma is a rare disorder with unremarkable, non-specific clinical manifestations in the oral cavity, which makes the diagnosis of this disease more difficult. We, therefore, report one case of this disease. Owing to the fact that diagnosis of this variant was mainly based on the positive findings of vimentin and S-100 in the immunohistochemistry examination and intracellular premelanosome detected by electron microscopy, immunodiagnosis and electron microscopy seem to be essential for differential diagnosis.

Topics: Adult; Diagnosis, Differential; Humans; Immunoenzyme Techniques; Keratins; Male; Melanoma; Microscopy, Electron; Palatal Neoplasms; S100 Proteins; Vimentin

This study examines the expression of anchorage independence and tumorigenicity in early cultures of oral rat keratinocytes. The epithelial cell lines originated from the palatal and the lingual mucosa of rats that had been painted with the carcinogen 4-nitroquinoline N-oxide. The colony forming efficiency (CFE) in gel culture of the cell lines derived from five squamous cell carcinomas of the tongue and palate predominantly increased with passage in culture. Carcinoma-derived cell lines that had a relatively high CFE (greater than 2.5%) formed tumours when transplanted to athymic mice, but cells in which the CFE was less than 2.5% were non-tumorigenic. Keratinocytes from a dysplastic palatal lesion were immortal, anchorage dependent and non-tumorigenic. A lingual papilloma cell line consistently expressed a very low CFE but was tumorigenic at the higher culture passages. The results show that the routine passage of cells in culture leads to the emergence of the anchorage independent and tumorigenic phenotypes in keratinocytes of malignant origin and, further, suggest that anchorage independence and tumorigenicity may exist as distinct phenotypes, with anchorage independence preceding tumorigenicity.

Topics: Animals; Carcinoma, Squamous Cell; Cell Adhesion; Epidermal Cells; Keratins; Mice; Mice, Nude; Neoplasms, Experimental; Palatal Neoplasms; Papilloma; Phenotype; Rats; Rats, Inbred Strains; Tongue Neoplasms; Tumor Cells, Cultured

Major histocompatibility complex (MHC) antigen expression has been postulated to have an important role in host defences against tumour development. In this study the expression of MHC class I and class II antigens in vitro, both constitutive and in response to interferon gamma (IFN gamma), was examined in a series of cell lines established from a rat model of oral carcinogenesis. Constitutive expression of MHC class I and class II antigens was not related to the degree of malignancy of the cell lines, as reflected by their anchorage independence in agarose gels and their capacity to form tumours in athymic mice. MHC class I response to IFN gamma stimulation did not correlate with tumorigenicity, but the MHC class II response was significantly decreased in one of the four tumorigenic cell lines. The results show that the expression of MHC antigens in response to IFN gamma varied between different keratinocyte cell lines but did not consistently reflect the tumorigenic phenotype.

Topics: Animals; Antigens, Surface; Cell Line; Epidermis; Histocompatibility Antigens Class I; Histocompatibility Antigens Class II; Interferon-gamma; Keratins; Palatal Neoplasms; Rats; Rats, Inbred Strains; Tongue Neoplasms; Tumor Stem Cell Assay

Two malignant mixed tumours, in which both carcinomatous and sarcomatous features were present, are described. They arose in the palate in patients who had undergone surgery and irradiation for a pleomorphic adenoma at the same site 30 and 36 years previously. The histological differential diagnoses of recurrent benign pleomorphic adenoma, pleomorphic adenoma resembling mesenchymal tumour, and carcinoma in (ex) pleomorphic adenoma are discussed. On the basis of their positive reaction for keratin with specific monoclonal antibodies it is suggested that the myoepithelial cells are of epithelial origin. Immunohistochemical studies together with the histological appearance of the neoplasms indicate that the carcinomatous as well as the sarcomatous elements were derived from modified myoepithelial tumour cells. Irradiation may have been responsible for inducing a true malignant mixed tumour as distinct from the more common malignancy which may arise in pleomorphic adenoma, this being a simple carcinoma.

Topics: Adenoma, Pleomorphic; Adult; Aged; Antigens, Neoplasm; Carcinoma, Intraductal, Noninfiltrating; Chondrosarcoma; Female; Humans; Keratins; Middle Aged; Neoplasms, Radiation-Induced; Palatal Neoplasms; Salivary Gland Neoplasms

Salivary gland myoepitheliomas are rare tumors accounting for less than 1% of neoplasms of the salivary glands. We report a myoepithelioma arising in the palate of an 8-year-old female, the youngest case in the literature. Electron microscopic and immunohistochemical studies support the myoepithelial origin of this tumor.

Topics: Carcinoembryonic Antigen; Child; Female; Fluorescent Antibody Technique; Humans; Keratins; Myoepithelioma; Palatal Neoplasms; S100 Proteins; Vimentin

Comparison was made of the electrophoretic keratin polypeptide patterns of normal hard palate epithelia from three hamsters and of eight palatal squamous cell carcinomas induced by 7,12-dimethylbenz(a)anthracene (DMBA) treatment. Keratin polypeptides from normal epithelia had a molecular weight range of about 48,000 to 70,000. In the tumour extracts, the large polypeptides (above 61,000) found in the normal epithelia were absent, but the majority of other small polypeptides below 61,000 were expressed. Three as yet undefined polypeptides, in the range of 40,000 to 70,000, were detected in tumour extracts, but not in extracts of normal palatal mucosa. The keratin polypeptide electrophoretic alterations in carcinomas of hamster palatal mucosa are similar to those reported for extra-oral carcinomas in other animal species.

Topics: 9,10-Dimethyl-1,2-benzanthracene; Animals; Carcinoma, Squamous Cell; Cricetinae; Electrophoresis, Polyacrylamide Gel; Keratins; Male; Mesocricetus; Neoplasm Proteins; Palatal Neoplasms; Palate; Peptides

Topics: Aspirin; Candidiasis; Cheilitis; Denture, Complete; Diagnosis, Oral; Foreign Bodies; Hyperplasia; Keratins; Leukoplakia; Lichen Planus; Palatal Neoplasms; Phenols; Salivary Duct Calculi; Stomatitis; Stomatitis, Aphthous; Stomatitis, Denture

Oral carcinoma in situ (CIS) as a histopathologic entity was studied in seventy-seven patients to determine the clinical and histologic parameters of the disease. There were forty-nine male and twenty-eight female patients, with 45.1 per cent of the lesions being described clinically as white, 15.9 per cent as red, and 8.5 per cent as a combination of the two. The high-risk sites for CIS were floor of the mouth (23.2 per cent of all lesions), tongue (22.0 per cent), and lips (in males only, 19.5 per cent). Histologically, there was a considerable range of variation in surface keratinization, thickness of epithelium, and certain cytologic alterations. The most consistent of all cytologic changes was loss of orientation of cells. There is no information available concerning possible regression of oral CIS, as is known for CIS of uterine cervix. Furthermore, there is no information concerning the frequency of or the period of transition from oral CIS to invasive carcinoma or whether all oral carcinoma is preceded by CIS. Further studies on this disease are essential.

Topics: Adult; Aged; Carcinoma in Situ; Cell Nucleus; Color; Cytoplasm; Epithelial Cells; Female; Humans; Keratins; Lip Neoplasms; Male; Middle Aged; Mouth Floor; Mouth Mucosa; Mouth Neoplasms; Palatal Neoplasms; Precancerous Conditions; Sex Factors; Tongue Neoplasms

Topics: Animals; Antibiotics, Antineoplastic; Biopsy; Bleomycin; Carcinoma, Squamous Cell; Culture Techniques; Desmosomes; Ear Neoplasms; Epiglottis; Glycogen; Humans; Keratins; Laryngeal Neoplasms; Maxillary Neoplasms; Mice; Microscopy, Electron; Mouth Neoplasms; Neoplasms, Experimental; Palatal Neoplasms; Tongue Neoplasms; Tonsillar Neoplasms

A correlative histocytological study was made of 6 patients with palatal carcinomata and 342 patients with palatal lesions (primarily leukoplakias) associated with reverse smoking from the Srikakulam district of Andhra Pradesh. Among 6 histologically diagnosed carcinomata only 2 showed cytological findings typical of carcinoma. Of the 46 atypias diagnosed histologically among the other palatal lesions, only 6 (13%) were diagnosed cytologically. Our findings show that cytological examination of precancerous and cancerous lesions located on the hard palate, which is a highly keratinized area of the oral cavity, may not be reliable enough for revealing premalignant or malignant changes.

Topics: Cytodiagnosis; Epithelium; Humans; India; Keratins; Leukoplakia, Oral; Palatal Neoplasms; Palate; Precancerous Conditions; Smoking