bromochloroacetic-acid and Nonodontogenic-Cysts

Topics: Autoradiography; Carcinoma, Transitional Cell; Cytodiagnosis; Dentigerous Cyst; Electrophoresis, Polyacrylamide Gel; Humans; Keratins; Nonodontogenic Cysts; Odontogenic Cysts; Recurrence

The aim of this study is to characterize immunohistochemical profiles of lining epithelia of nasopalatine duct cyst (NPC) as well as to correlate those findings with their clinicopathological features to understand the histopathogenesis of NPC.. Forty-one surgical specimens from NPC were examined for clinical profiles and expression of keratin-7, 13, MUC-1, and P63 by immunohistochemistry, compared to radicular cyst (RC) and maxillary sinusitis.. Nasopalatine duct cyst was clinically characterized by male predominant occurrence: 44% of the cases involved tooth roots, and 70% with inflammatory backgrounds. Lining epithelia of NPCs without daughter cysts were immunohistochemically distinguished into three layers: a keratin 7-positive (+) ciliated cell layer in the surface, a keratin-13+ middle layer, and a MUC-1+/P63+ lower half, indicating that they were not respiratory epithelia, and the same layering pattern was observed in RC. However, those immunolocalization patterns of the main cyst lining with daughter cyst were exactly the same as those of daughter cyst linings as well as duct epithelia of mucous glands.. Two possible histopathogenesis of NPC were clarified: one was inflammatory cyst like RC and the other was salivary duct cyst-like mucocele.

Topics: Adult; Aged; Epithelial Cells; Female; Humans; Inflammation; Keratins; Male; Maxillary Diseases; Maxillary Sinusitis; Membrane Proteins; Middle Aged; Mucins; Mucocele; Nasal Cavity; Nonodontogenic Cysts; Palate, Hard; Radicular Cyst; Sex Ratio; Terminology as Topic; Tooth Root; Young Adult

Topics: Diagnosis, Differential; Humans; Keratins; Male; Maxillary Diseases; Middle Aged; Nonodontogenic Cysts; Palate; Tomography, X-Ray Computed

Cytokeratin (CK) expression patterns have been studied in numerous intact and diseased oral tissues. However, CK expression in metaplastic squamous cells has not been explored in depth and the origin of metaplastic epithelial linings of the jaw cysts has not been sufficiently investigated.. We examined CK expression in 46 postoperative maxillary cysts (POMCs) which were lined with pseudostratified columnar cells only, columnar and squamous cells, and squamous cells only, in 13, 30 and 3 cases, respectively.. The expression of CK8, CK13 and CK18 were observed in 39, 9 and all 43 of the columnar epithelial linings, respectively. Metaplastic squamous epithelia expressed more CK13, and less CK18 and CK8. Of the 33 metaplastic linings, 24 expressed CK8, 23 CK13 and 26 linings expressed CK18. The patterns of expression of CK13 and CK18 observed were CK18(+)-CK13(-) in 10 metaplastic linings, CK18(+)-CK13(+) in 16, and CK18(-)-CK13(+) in 7. The expression of CK13- and CK18-mRNA was generally correlated with level of protein expressed. CK18-mRNA expression was observed by in situ hybridization, not only in the 26 metaplastic linings which were positive for CK18 protein, but also in five of the seven metaplastic linings which did not express CK18 protein. In addition, RT-PCR revealed an expression of CK18-mRNA in all metaplastic squamous linings, although the expression level was weaker than that in the columnar epithelial linings. The CK13-mRNA was expressed inversely to the CK18-mRNA.. These results indicate that CK18-mRNA is preserved through metaplasia, although the protein expression decreased. Metaplastic squamous cells differentiate with a decrease of CK18 and an increase of CK13 expression.

Topics: Cell Differentiation; Epithelial Cells; Gene Expression; Humans; Immunohistochemistry; In Situ Hybridization; Isoelectric Focusing; Keratins; Maxillary Diseases; Metaplasia; Nonodontogenic Cysts; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger

The botryoid odontogenic cyst (BOC) is considered a rare multilocular variant of the lateral periodontal cyst. The origin of the BOC can be seen in aberrant odontogenic tissue. The BOC is found especially in the premolar region of the mandible, as well as in the frontal region of the maxilla of patients aged between 60 and 70 years. Most of the 11 published articles of BOC have shown high rates of recurrence. Histopathologically the BOC is marked by multilocular cysts lined by a thin, nonkeratinized epithelium. Clusters of glycogen-rich epithelial cells may be noted in nodular thickenings of the cyst lining. For the clinician, the differentiation of the BOC from the keratocyst and ameloblastoma is relevant. One case of a large BOC (65-year-old male, BOC regio 33-45, diameter 5 cm, radiographically and histologically multilocular) is presented with a review of the literature, including the therapeutic management, and the possible diagnostic criteria are discussed. The immunohistochemically determined expression of cytokeratin (CK) 13 implicates the histogenetic origin of the BOC from the squamous epithelium of the oral cavity and excludes the origin from the small salivary glands. The expression of CK 19 and the lack of expression of p53, as well as the higher proliferation rate of the basal epithelial cell layer by the BOC, may be useful for distinction between the keratocyst.

Topics: Ameloblastoma; Biomarkers, Tumor; Diagnosis, Differential; Humans; Keratins; Male; Mandibular Diseases; Mandibular Neoplasms; Middle Aged; Nonodontogenic Cysts; Odontogenic Cysts

We have identified strong expression of a 38-kD cell surface glycoprotein (gp38), a marker of basal cell carcinomas (BCCs), in basal and suprabasal epithelial cell membranes of parakeratinised odontogenic keratocysts. In contrast, orthokeratinised cysts and most other odontogenic cyst types, ameloblastomas, normal stratified oral epithelium, cell rests of Malassez and glands of Serres, all proved negative. To our knowledge this is the first histochemical marker to distinguish between these major cyst types. It has obvious uses in the diagnosis of inflamed keratocysts and the separation of ameloblastomas from BCCs and may find a role in studies of the developmental biology of other odontogenic structures.

Topics: Antibodies, Monoclonal; Biomarkers; Biomarkers, Tumor; Carcinoma, Basal Cell; Dental Enamel; Epithelium; Gene Expression Regulation; Glycoproteins; Humans; Keratins; Membrane Glycoproteins; Mouth Mucosa; Nonodontogenic Cysts; Odontogenic Cysts; Periapical Granuloma; Periodontitis

Keratins and secretory component (SC) were immunohistochemically examined in fresh tissue samples from 45 odontogenic and 35 non-odontogenic cysts. Lining epithelia of almost all cases contained keratins which reacted with polyclonal antibodies (Dako, Bio-Science), and no difference could be found between the two groups of lesions. By staining with two monoclonal antibodies against keratins, i.e., RGE53 (Bio-Science) and RKSE60 (Bio-Science), it was revealed that the epithelia of non-odontogenic cysts, which were columnar epithelium in most cases, had fully and positively reacted with RGE53, while none of the cases was positive for RKSE60. In contrast, the squamous linings of odontogenic cysts except for two cases did not react with RGE53, and few cases possessed RKSE60-reactive keratin. SC was also contradictory. All non-odontogenic cysts exhibited SC. Regarding each pair of non-odontogenic and odontogenic cysts covered with RGE53 and SC-positive, and RKSE60-negative squamous epithelium, it seemed reasonable from the staining results to conclude that the squamous linings were metaplastic from the columnar epithelium. Based on the results, concomitant examinations of SC with keratins will be helpful in deciding the epithelial derivation of jaw cysts.

Topics: Dentigerous Cyst; Epithelium; Humans; Immunohistochemistry; Jaw Cysts; Keratins; Metaplasia; Molecular Weight; Nonodontogenic Cysts; Odontogenic Cysts; Radicular Cyst; Secretory Component

The patterns of cytokeratin expression in the epithelium of 5 dental follicles, 7 dentigerous cysts, 5 odontogenic keratocysts, 3 nasopalatine cysts and an epidermoid cyst have been studies using a panel of monoclonal antibodies. The epithelium of dental follicles and of developmental odontogenic cysts strongly expressed keratins 5 and 19 and showed weaker expression of keratins typical of stratified non-cornified and of simple epithelia. Staining with mAbs against the latter keratins varied with the degree of epithelial differentiation. Nasopalatine cysts strongly expressed simple epithelial keratins and the epidermoid cyst strongly expressed a marker of cornification. Odontogenic cysts thus appear to differ in their pattern of keratin expression from other oral developmental cysts and all derivatives of odontogenic epithelia appear to share similar basic patterns of cytokeratin expression.

Topics: Antibodies, Monoclonal; Dental Sac; Dentigerous Cyst; Epidermal Cyst; Epithelium; Fluorescent Antibody Technique; Humans; Immunoenzyme Techniques; Keratins; Nonodontogenic Cysts; Odontogenic Cysts; Tooth Germ

Topics: Bone Cysts; Cysts; Dentigerous Cyst; Humans; Keratins; Mouth Diseases; Nonodontogenic Cysts; Odontogenic Cysts; Radicular Cyst; Radiography

Topics: Jaw Neoplasms; Keratins; Nonodontogenic Cysts; Odontogenic Cysts

Topics: Adult; Aged; Animals; Cysts; Dentigerous Cyst; Female; Fetus; Haplorhini; Humans; Jaw Diseases; Jaw Neoplasms; Keratins; Macaca; Male; Mandible; Middle Aged; Molar; Mouth Mucosa; Nonodontogenic Cysts; Pregnancy

Topics: Acetates; Adult; Animals; Biopsy, Needle; Blood Donors; Blood Proteins; Cattle; Cellulose; Child; Dentigerous Cyst; Electrophoresis; Humans; Hyaluronoglucosaminidase; Immunoglobulins; Jaw Neoplasms; Keratins; Nonodontogenic Cysts; Periodontal Cyst; Proteins; Viscosity

Topics: Keratins; Nonodontogenic Cysts

Topics: Adolescent; Adult; Child; Dentigerous Cyst; Humans; Jaw Diseases; Jaw Neoplasms; Keratins; Middle Aged; Nonodontogenic Cysts; Odontogenic Cysts; Radicular Cyst; Radiography; Recurrence

Topics: Bone Cysts; Dentigerous Cyst; Humans; Jaw Diseases; Jaw Neoplasms; Keratins; Lymphatic System; Methods; Nonodontogenic Cysts; Odontogenic Cysts; Osmosis; Recurrence