bromochloroacetic-acid and Mixed-Tumor--Malignant

Renal mixed tumor characterized by the absence of nephrogenic blastema and the presence of predominant osteoid-producing osteoblast-like cells occurred in the kidney of a 6-month-old, hybrid, female pig. At the post-mortem examination, the tumor was found as a calcified grayish-white mass at the cranial end of the left kidney. Histologically the tumor consisted of 3 growth areas of poorly differentiated spindle cells, osteoid-producing osteoblast-like cells, and luminal epithelial cells. Transition from the spindle cells to the osteoblast-like cells or the luminal epithelial cells was observed. Immunohistochemically, the spindle cells and the osteoblast-like cells were consistently positive for β-catenin. Although the luminal epithelial cells and adjacent spindle cells were positive for cytokeratin, these 3 types of tumor cells were consistently negative for WT1. The tumor was diagnosed as primary renal mixed tumor characterized by marked proliferation of osteoblast-like cells with osteoid formation.

Topics: Animals; beta Catenin; Cell Proliferation; Epithelial Cells; Female; Immunohistochemistry; Keratins; Kidney Neoplasms; Mixed Tumor, Malignant; Osteoblasts; Swine; Swine Diseases

Topics: Biomarkers, Tumor; Bone Neoplasms; Calcium-Binding Proteins; Calponins; Chondroblastoma; Diagnosis, Differential; Humans; Immunohistochemistry; Keratins; Magnetic Resonance Imaging; Male; Membrane Proteins; Microfilament Proteins; Middle Aged; Mixed Tumor, Malignant; S100 Proteins; Soft Tissue Neoplasms; Vascular Neoplasms

Metaplastic breast carcinoma (MBC) is a rare, heterogeneous breast cancer characterized by admixture of adenocarcinoma with metaplastic elements, low hormone receptor expression, and poor outcomes. The authors retrospectively reviewed the medical records of 47 MBC patients and 1,346 invasive ductal carcinoma (IDC) patients. Two hundred eighteen of the IDC patients were triple-negative (TN-IDC) for estrogen receptor, progesterone receptor, and human epidermal growth factor receptor-2 (ER-/PR-/HER2-). Patients were surgically treated at the Samsung Medical Center between 2005 and 2009. The MBC patients presented with a larger tumor size, lower lymph node involvement, higher histological and nuclear grades, higher triple negativity (ER-/PR-/HER2-) and higher p53, CK5/6, and EGFR expressions compared with those of the IDC group. However, there were no significant differences in clinicopathological characteristics between MBC and TN-IDC. During the follow-up period (median duration of 30.3 months, range 2.6-56.3 months), seven (14.9%) MBC patients, and 98 (7.1%) IDC patients had disease recurrence. The three-year disease-free survival (DFS) rate was 78.1% in the MBC group and 91.1% in IDC group (P < 0.001). The three-year DFS rate was not significantly different between the MBC and TN-IDC groups (78.1 vs. 84.9%, P = 0.114). However, in patients with lymph node metastasis who underwent adjuvant chemotherapy, the three-year DFS rate was 44.4% in the MBC group and 72.5% in the TN-IDC group (P = 0.025). The authors found that MBC had a poorer clinical outcome than did IDC. In breast cancer patients with nodal metastasis, MBC had a poorer prognosis than did TN-IDC, despite adjuvant chemotherapy.

Topics: Adult; Breast Neoplasms; Carcinoma, Ductal, Breast; Disease-Free Survival; ErbB Receptors; Female; Humans; Keratins; Ki-67 Antigen; Lymphatic Metastasis; Middle Aged; Mixed Tumor, Malignant; Multivariate Analysis; Neoplasm Staging; Prognosis; Receptor, ErbB-2; Receptors, Estrogen; Receptors, Progesterone; Retrospective Studies; Tumor Suppressor Protein p53

Topics: Aged, 80 and over; Biomarkers, Tumor; Carcinoma, Renal Cell; Female; Humans; Keratins; Kidney; Kidney Neoplasms; Mixed Tumor, Malignant; Tomography, X-Ray Computed; Treatment Outcome

Topics: Antibodies, Monoclonal; Humans; Immunohistochemistry; Keratin-15; Keratins; Mixed Tumor, Malignant; Peptides; Skin Neoplasms

We report a case of mixed tumor arising in the lower vaginal wall. The patient was a 20-yr-old nullipararous woman. The tumor was relatively well-defined with expansile margin, and showed solid sheets or fascicles of stromal-type spindle cells and ovoid epithelial cells with sparsely scattered nests of mature squamous epithelium and glands lined by mucinous epithelium. Cellular atypia was not conspicuous, however, mitosis was counted upto 6 per 10 high power fields. We examined this tumor immunohistochemically and ultrastructurally and reviewed the articles to identify the histogenesis. Positive reaction for vimenin and cytokeratin of stromaltype spindle cells and presence of desmosome-like structures and tonofilaments on electron microscopic examination suggested the epithelial origin of the stromaltype spindle cells.

Topics: Adult; Female; Humans; Immunohistochemistry; Keratins; Microscopy, Electron; Mitosis; Mixed Tumor, Malignant; Vagina; Vaginal Neoplasms; Vimentin

Malignant mixed tumors in the vagina are extremely rare. We experienced a case of a malignant mixed tumor (synovioid variant). Surgical treatment was performed, followed by 3 courses of chemotherapy. Up to the present time, 4 years after the first treatment, no signs of recurrence have been observed.

Topics: Biopsy; Carcinoembryonic Antigen; Chemotherapy, Adjuvant; Female; Follow-Up Studies; Humans; Hysterectomy; Immunohistochemistry; Keratins; Middle Aged; Mixed Tumor, Malignant; Periodic Acid-Schiff Reaction; Vagina; Vaginal Neoplasms

Topics: Diagnosis, Differential; Humans; Keratins; Mixed Tumor, Malignant; Myoepithelioma; S100 Proteins; Soft Tissue Neoplasms

Tumor cells from a malignant mixed tumor of the lacrimal gland were maintained in tissue culture for more than 55 generations. Comparative immunohistochemical analysis was performed on whole tumor sections and on the tumor cell culture to define the origin of the cells in culture. The cultured cells expressed cytokeratin, smooth-muscle actin, S-100 protein, and vimentin and were negative for glial fibrillary acidic protein. Tumor sections expressed cytokeratin but were negative for muscle-specific actin, vimentin, and glial fibrillary acidic protein. Through tissue culture studies of salivary gland epithelial neoplasias, which are very similar to lacrimal gland epithelial neoplasias, pluripotential stem cells have been identified. Similar tissue culture analysis of lacrimal gland epithelial neoplasms can be a valuable tool for studying the origin of these uncommon tumors.

Topics: Antigens, Neoplasm; Biomarkers, Tumor; Chromosome Aberrations; Chromosome Disorders; Eye Neoplasms; Humans; Immunohistochemistry; Karyotyping; Keratins; Lacrimal Apparatus; Lacrimal Apparatus Diseases; Male; Mixed Tumor, Malignant; Tumor Cells, Cultured

Primary lung tumors showing features of salivary gland-type neoplasms are extremely rare.. Eight patients with primary lung neoplasms showing light microscopic and immunohistochemical features of salivary gland-type mixed tumors were studied.. The patients were six women and two men, ages 35-69 years (mean, 52.5 years). The tumors ranged from 2 to 16 cm in greatest diameter. In two patients the lesions presented as polypoid endobronchial lesions obstructing the lumen; in another two patients the lesions were found in close proximity or in continuity with a bronchus; in three patients, the lesions presented as peripheral parenchymatous nodules unrelated to a bronchus; and in one patient, the relationship to the bronchus could not be determined. Histologically, the lesions were biphasic, showing admixtures in varying proportions of epithelial elements containing a predominant myoepithelial cell population with a stromal component containing an abundant myxoid or focally chondroid matrix. Immunohistochemical studies showed strong positivity of the cells in the epithelial component with low molecular weight keratins (CAM 5.2), and to a lesser extent with broad spectrum keratin, actin, and vimentin antibodies. The cells also showed variable reactivity in the epithelial and nonepithelial elements with S-100 protein and glial fibrillary acidic protein. Six tumors were grossly and histologically benign; in two patients, the tumors were larger, locally invasive, and showed more atypical histologic features. All patients were treated with surgical excision. On follow-up, of the six patients with histologically benign-appearing tumors, one was alive and well 6 years after surgery; another died 4 years after surgery of a second unrelated malignancy; one died during the immediate postoperative period of myocardial infarction; and three have been lost to follow-up. In the two patients with histologically atypical lesions, the tumors recurred and metastasized after 2 and 3 years, respectively, with one of them leading to death caused by widespread metastases and superior vena cava syndrome.. Review of the literature and the findings in the current series indicate that salivary gland-type mixed tumors of the lung may present with a spectrum of histologic features and clinical behavior, ranging from benign to frankly malignant, similar to that observed for their salivary gland counterparts. Size of the lesion at the time of presentation, extent of local infiltration, and degree of mitotic activity appear to be the most reliable prognostic features of these tumors.

Topics: Actins; Adenoma, Pleomorphic; Adult; Aged; Female; Glial Fibrillary Acidic Protein; Humans; Immunohistochemistry; Keratins; Lung Neoplasms; Male; Middle Aged; Mixed Tumor, Malignant; S100 Proteins; Vimentin