bromochloroacetic-acid and Joint-Diseases

Assessment of antikeratin antibodies (AKAs), using an indirect immunofluorescence technique with rat esophageal keratin as antigen, was performed in 14 patients with arthropathy and palmoplantar pustular eruptions, six of whom also had psoriasis vulgaris. Twelve patients had seronegative spondyloarthropathy. They were all AKA negative. Two patients had classical seropositive-erosive rheumatoid arthritis (RA), and were both AKA positive. This suggests that AKA is not related to the arthropathy associated with pustulosis palmoplantaris or psoriasis, or to the presence of pustular eruptions on the palms and soles. The finding of AKA in RA is in keeping with previous findings.

Topics: Aged; Autoantibodies; Dermatitis; Female; Humans; Immunoglobulin G; Joint Diseases; Keratins; Male; Middle Aged; Psoriasis

Seven cases of synovial sarcoma (SS), two with biphasic and five with monophasic histology, were studied immunohistochemically using monoclonal antibodies to intermediate filaments, keratin, and vimentin. Slender spindle cells and plump cells were constant components of all the tumors. Epithelioid cells were present only in biphasic SS. Epithelioid cells and plump cells were positively stained by both keratin and vimentin. Slender spindle cells were stained positive for vimentin and negative for keratin. Staining manner of each cell type was similar irrespective of monophasic or biphasic pattern. Present immunohistochemical studies suggested that monophasic or biphasic patterns in SS should be regarded as a different expression of the same disease. In addition, immunohistochemistry proved to be a useful tool to detect plump cells which were difficult to find on routine staining.

Topics: Adolescent; Adult; Female; Foot; Hand; Humans; Immunoenzyme Techniques; Joint Diseases; Keratins; Knee Joint; Male; Sarcoma, Synovial; Soft Tissue Neoplasms; Vimentin

Nineteen synovial sarcomas, six biphasic and 13 monophasic tumors, were examined by light and electron microscopy and immunohistochemically for the presence of the epithelial markers keratin and epithelial membrane antigen (EMA). Ultrastructurally, intercellular spaces with processes are present to varying degrees in the spindle cell component of all synovial sarcomas, and junctional specializations occur in most cases. Tumors of the two types differ in their content of external (basal) lamina, which encloses the epithelial component of all biphasic tumors and is detectable in the spindle cell component of two thirds of them, but is absent from the majority of monophasic tumors. Keratin and EMA were demonstrated in both components of all six biphasic tumors. Of the 13 monophasic tumors, keratin was present in nine, EMA in eight, and at least one epithelial marker in ten. Synovial sarcoma is regarded as a distinctive soft tissue tumor with variable epithelial-like differentiation. The use of electron microscopy can increase the specificity of immunohistochemical studies of soft tissue sarcomas and allow more accurate differentiation of monophasic synovial sarcoma from other spindle cell tumors, particularly those that do not express markers.

Topics: Epithelium; Histocytochemistry; Humans; Immunochemistry; Joint Diseases; Keratins; Membrane Proteins; Microscopy, Electron; Mucin-1; Sarcoma; Soft Tissue Neoplasms; Synovial Membrane

Topics: Female; Humans; Joint Diseases; Keratins; Middle Aged; Neoplasms; Sarcoma; Synovial Membrane

Monophasic synovial sarcomas of spindle-cell type and fibrosarcomas were studied by electron and immunofluorescence microscopy for their intermediate filament expression and the binding of peanut agglutinin (PNA). In monophasic synovial sarcomas of spindle-cell type (two cases), frequent cell-to-cell junctions, irregular cytoplasmic processes, and occasional cytoplasmic, tonofilament-like bundles of intermediate filaments were seen by electron microscopy. These features were absent from fibrosarcomas. Immunohistologically, the monophasic synovial sarcomas showed arrays of prekeratin-positive cells in the midst of the vimentin-positive spindle cells. By double fluorescence microscopy, the prekeratin-positive cells also bound PNA, like the epithelial-like cells of the classical biphasic synovial sarcoma. In contrast to monophasic synovial sarcomas, prekeratin-positive cells and arrays of PNA-binding cells, were not seen by immunofluorescence microscopy in fibrosarcomas (seven cases). Thus the prekeratin-content, the binding of PNA lectin, and certain ultrastructural features suggesting early epithelial differentiation, help to distinguish monophasic synovial sarcomas of spindle-cell type from other spindle cell sarcomas.

Topics: Adult; Binding Sites; Cytoskeleton; Elbow Joint; Female; Fibrosarcoma; Finger Joint; Histocytochemistry; Humans; Intermediate Filament Proteins; Joint Diseases; Keratins; Lectins; Male; Microscopy, Electron; Protein Precursors; Sarcoma, Synovial; Vimentin

Topics: Acrylamides; Animals; Aorta; Artiodactyla; Cartilage, Articular; Centrifugation, Density Gradient; Chondroitin; Densitometry; Drug Stability; Electrophoresis; Female; Gels; Glycoproteins; Glycosaminoglycans; Humans; Hyaluronic Acid; Joint Diseases; Keratins; Molecular Weight; Polysaccharides; Pregnancy; Solubility; Sulfuric Acids; Swine; Umbilical Cord

Topics: Animals; Cartilage, Articular; Chondroitin; Guinea Pigs; Joint Diseases; Keratins

Topics: Biomechanical Phenomena; Cartilage Diseases; Cartilage, Articular; Chondroitin; Glycosaminoglycans; Humans; Hydroxyproline; Joint Diseases; Keratins