bromochloroacetic-acid and Infant--Newborn--Diseases

Skin biopsy samples and amniotic fluid cells obtained in utero from two fetuses at risk for epidermolytic hyperkeratosis were examined by light and electron microscopy. Both fetuses were affected; the second was carried to term. Epidermal extracts were prepared from blisters of the newborn for analysis of keratin and filaggrin proteins. Abnormal clumps of keratin filaments were present in all layers of the prekeratinized fetal epidermis except the periderm and stratum germinativum. A significant population of amniotic fluid cells also contained the filament aggregations. Prenatal diagnosis of the disease should be possible using cells obtained at amniocentesis, thus avoiding fetal skin biopsy. Biochemical studies showed abnormalities in keratin and filaggrin proteins. The structural alterations in the tissue might be a consequence of altered interaction between these two abnormal epidermal proteins.

Topics: Amniotic Fluid; Cross Reactions; Cytoskeleton; Epidermis; Female; Filaggrin Proteins; Gestational Age; Humans; Infant, Newborn; Infant, Newborn, Diseases; Intermediate Filament Proteins; Keratins; Male; Molecular Weight; Pregnancy; Prenatal Diagnosis; Skin Diseases, Vesiculobullous

Topics: Birth Injuries; Blister; Drug Eruptions; Epidermolysis Bullosa; Erythema; Female; Herpes Simplex; Humans; Infant; Infant, Newborn; Infant, Newborn, Diseases; Keratins; Listeria monocytogenes; Lupus Erythematosus, Systemic; Male; Maternal-Fetal Exchange; Miliaria; Nevus; Pregnancy; Pregnancy Complications; Rubella; Skin Diseases; Skin Diseases, Infectious; Stevens-Johnson Syndrome; Sucking Behavior; Syphilis, Congenital; Urticaria Pigmentosa

Topics: Bone and Bones; Epithelium; Female; Fingers; Humans; Ichthyosis; Infant, Newborn; Infant, Newborn, Diseases; Keratins; Meconium; Muscles; Scalp; Skin; Thymus Gland; X-Ray Diffraction

Topics: Ectropion; Humans; Ichthyosis; Infant; Infant, Newborn; Infant, Newborn, Diseases; Keratins; Male