bromochloroacetic-acid and Histiocytosis--Langerhans-Cell

In all mammalian species so far examined, Langerhans cells or their precursors are the only epidermal cells expressing Ia antigens or their equivalents. In man, xeno-antisera raised in rabbits against purified B-lymphocyte cell membrane antigens were utilized to stain the Langerhans cells by either fluorescent or immunoferritin methods. As high proportion of the indeterminate cells in the epidermis also expressed HLA-DR antigens, and a relationship to Langerhans cells is suggested. Confirmation of these results was obtained in mouse. Alloantisera raised against I-A and I-EC subregion products again stained only Langerhans cells. Fluorescent, immunoperoxidase, and immunoferritin methods were used and confirmation of the specificity of the reaction was achieved at the electron microscope level. Langerhans cells were shown by ATPase staining to be absent from the epithelium of the central cornea, but present in the limbus. Population of the entire corneal epithelium surface was induced by application of irritants or contact sensitizing agents such as DNCB. Grafting of corneas either deficient or populated with Langerhans cells, to skin beds, may answer the question of the influence of such cells on allograft rejection.

Topics: Animals; Behcet Syndrome; Cattle; Cell Communication; Chickens; Chiroptera; Cricetinae; Dermatitis, Contact; Female; Guinea Pigs; Histiocytosis, Langerhans-Cell; Histocompatibility Antigens Class II; HLA Antigens; Humans; Keratins; Langerhans Cells; Lorisidae; Lymph Nodes; Lymphocytes; Mice; Mitosis; Mycosis Fungoides; Rabbits; Rats; Receptors, Immunologic; Sheep; Stomatitis, Aphthous; Swine; T-Lymphocytes; Thymus Gland

Langerhans cell histiocytosis (LCH) involvement of the gastrointestinal tract (GIT) is rare. The infiltrate in a colonic biopsy can be dissimilar to classic cytomorphologic features. It could be patchy, restricted to the subepithelial lamina propria and the lesional cells might have prominent nucleoli with lymphocytes-dominant background. The GIT manifestations could be confused with infectious, allergic, immunodeficiency and inflammatory bowel diseases. The rarity of GIT lesions, unawareness of some atypical endoscopic and histologic features might lead to false negative results. We report a case of LCH in an 11-month-old baby that was clinically unsuspected and histologically overlooked.

Topics: Antigens, CD; Antigens, CD1; Antigens, Differentiation, Myelomonocytic; Biopsy; Colon; Colonic Diseases; Diagnostic Errors; Gastrointestinal Diseases; Histiocytosis, Langerhans-Cell; Humans; Immunohistochemistry; Infant; Keratins; Male; Peroxidase; S100 Proteins

Topics: Dendritic Cells; Histiocytes; Histiocytosis, Langerhans-Cell; Humans; Keratins; Langerhans Cells; Lymphoma, Large B-Cell, Diffuse; Sarcoma

Topics: Antibodies, Monoclonal; Dermatitis; Histiocytosis, Langerhans-Cell; Histocytochemistry; Humans; Hypersensitivity, Delayed; Immunochemistry; Keratins; Skin Diseases; Skin Neoplasms; T-Lymphocytes