bromochloroacetic-acid and Guillain-Barre-Syndrome

Profile and immunoreactivity of proteins from HPN tissue, and from Campylobacter jejuni (O:19) were investigated. Proteins were extracted, separated by SDS-PAGE, their cross reactivity monitored by Western blotting, and identified by nHPLC-nESI-HRMS analysis. Proteins from C. jejuni, at Mw ~70 KDa were chaperone/co-chaperone proteins (GroEL, DnaK and HtpG). In the corresponding HPN band were serum albumin, neurofilament light peptide, cytoskeletal keratins and one HSP 70 and one HSP60. These chaperones reciprocally share high primary sequence homology and conservation of their known epitopes. These findings suggest that HSP chaperones may be suitable candidates involved in the molecular mimicry triggering GBS.

Topics: Campylobacter jejuni; Chromatography, High Pressure Liquid; Cross Reactions; Epitopes; Guillain-Barre Syndrome; Humans; Keratins; Molecular Chaperones; Molecular Mimicry; Molecular Weight; Nerve Tissue Proteins; Peripheral Nerves; Sequence Alignment; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry

The concurrent development of Guillain-Barré syndrome (GBS) and myasthenia gravis (MG) is rare. It has been associated with molecular mimicry between infectious agents and self-antigens. Such antibodies may show cross-reactions against both myelin proteins of peripheral nerves and acetylcholine receptors of neuromuscular junctions. Thymoma-associated multi-organ autoimmunity may also play a role in initiating autoimmune process. We present such a case with the concurrent development of GBS and MG.

Topics: Action Potentials; Adult; Antibodies; Electric Stimulation; Female; Guillain-Barre Syndrome; Humans; Keratins; Myasthenia Gravis; Neural Conduction; Reaction Time; Receptors, Cholinergic; Taiwan; Tomography, X-Ray Computed