bromochloroacetic-acid has been researched along with Fuchs--Endothelial-Dystrophy* in 3 studies
3 other study(ies) available for bromochloroacetic-acid and Fuchs--Endothelial-Dystrophy
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Primary Descemet's Membrane Endothelial Keratoplasty for Fuchs Endothelial Dystrophy versus Bullous Keratopathy: Histopathology and Clinical Results.
To investigate functional and anatomical results up to 12 months after Descemet's membrane endothelial keratoplasty (DMEK) for Fuchs' endothelial dystrophy (FED) versus bullous keratopathy (BK) in consideration of morphologic characteristics on host's endothelium-Descemet's membranes (EDM).. In a prospective consecutive case series, 119 eyes underwent a primary DMEK for FED or BK. Intraoperatively obtained EDM were investigated immunohistologically. Clinical and morphological parameters were compared between FED and BK.. DMEK produces similar results for FED and BK. However, the postoperative course may differ with regard to the recurrence of secondary graft detachments that may be associated by histopathologic particularities. Topics: Aged; Aged, 80 and over; Blister; Cell Count; Corneal Diseases; Descemet Membrane; Descemet Stripping Endothelial Keratoplasty; Endothelium, Corneal; Female; Fibronectins; Fluorescent Antibody Technique, Indirect; Fuchs' Endothelial Dystrophy; Graft Survival; Humans; Immunohistochemistry; Keratins; Male; Middle Aged; Prospective Studies; Refraction, Ocular; Tissue Donors; Visual Acuity | 2018 |
Culture of human corneal endothelial cells isolated from corneas with Fuchs endothelial corneal dystrophy.
The purpose of this study was to assess the feasibility of initiating primary cultures of corneal endothelial cells from patients suffering from Fuchs endothelial corneal dystrophy (FECD; MIM# 1036800). We also evaluated which conditions yielded the best results for culture. Twenty-nine patients undergoing Descemet stripping automated endothelial keratoplasty consented to the use of their excised Descemet's membrane for this study. Out of the 29 specimens, 18 successfully initiated a culture. Cell morphology varied between endothelial (rounded, slightly elongated cells, n = 12) and fibroblastic-like (thin and very elongated cells, n = 6). These differences in cell morphology were also observed with the normal human corneal endothelial cell cultures. The cultures that initially presented an endothelial morphology maintained their shape in subcultures. Clusterin expression was similar in FECD and normal endothelial cells. Transmission electron microscopy of FECD Descemet's membranes showed a high degree of various abnormalities generally found in this disease, such as a thickened Descemet's membrane, presence of a posterior banded layer, presence of a fibrillar layer and striated bodies of various sizes and periodicities. Patient's age was predictive of culture success, all younger FECD donors generating cultures of endothelial morphology. The absence of a fibrillar layer was also a factor associated with greater success. Culture success was not dependent on specimen size, specimen pigmentation, or patient's preoperative central corneal thickness. In conclusion, this paper shows for the first time that central Descemet's membranes of patients suffering from FECD possess proliferative endothelial cells that can be isolated and cultured without viral transduction, opening the way for new in vitro studies of this disease. Topics: Aged; Aged, 80 and over; Aging; Biomarkers; Cell Culture Techniques; Cell Proliferation; Cell Separation; Cell Shape; Clusterin; Descemet Membrane; Endothelium, Corneal; Female; Fluorescent Antibody Technique, Indirect; Fuchs' Endothelial Dystrophy; Humans; Keratins; Male; Middle Aged | 2012 |
Epithelial metaplasia of the corneal endothelium in Fuchs endothelial dystrophy.
To evaluate the immunohistochemical characteristics of human corneas with the diagnosis of Fuchs endothelial dystrophy (FED).. Formalin-fixed, paraffin-embedded sections of corneas with the diagnosis of FED (15 patients) and 10 control corneas were stained with hematoxylin-eosin and periodic acid-Schiff (PAS). Adjacent histologic sections were stained with monoclonal antibodies that react with epithelial antigens: pancytokeratin, cytokeratins (CK) 7 and 20 CAM 5.2, epithelial membrane antigen (EMA), and Ber EP4. Eight corneas were stained with antibodies to vimentin, smooth-muscle actin (SMA), and CD 68.. The endothelial cells in FED were attenuated and atrophic; some contained pigment consistent with melanin. The endothelial cells stained for pancytokeratin, CK 7, and vimentin in all corneas of FED, whereas variable staining was noted with CAM 5.2. No staining of endothelium was noted with CK 20, EMA, BerEP4, SMA, or CD 68.. Some cytokeratins that are normally restricted to true epithelium are present in the endothelium of FED. Epithelial metaplasia of endothelium in FED may represent a nonspecific response of distressed endothelial cells, as previously reported in posterior polymorphous dystrophy, congenital hereditary endothelial dystrophy, and iridocorneal endothelial syndrome. Topics: Actins; Aged; Biomarkers; Endothelium, Corneal; Epithelium, Corneal; Female; Fuchs' Endothelial Dystrophy; Humans; Keratin-20; Keratin-7; Keratins; Male; Metaplasia; Middle Aged; Mucin-1; Periodic Acid-Schiff Reaction; Vimentin | 2006 |