bromochloroacetic-acid and Dentigerous-Cyst

bromochloroacetic-acid has been researched along with Dentigerous-Cyst* in 38 studies

Reviews

1 review(s) available for bromochloroacetic-acid and Dentigerous-Cyst

ArticleYear
Newer concepts of odontogenic cysts.
    International journal of oral surgery, 1972, Volume: 1, Issue:1

    Topics: Autoradiography; Carcinoma, Transitional Cell; Cytodiagnosis; Dentigerous Cyst; Electrophoresis, Polyacrylamide Gel; Humans; Keratins; Nonodontogenic Cysts; Odontogenic Cysts; Recurrence

1972

Other Studies

37 other study(ies) available for bromochloroacetic-acid and Dentigerous-Cyst

ArticleYear
Markers of proliferation and cytokeratins in the differential diagnosis of jaw cysts.
    Ear, nose, & throat journal, 2017, Volume: 96, Issue:9

    We conducted a retrospective study to analyze the histologic and immunohistochemical findings in three main types of odontogenic cyst. We studied 90 archived cystic jaw lesions: 30 dentigerous cysts, 30 keratocystic odontogenic tumors, and 30 radicular cysts. The cyst types were identified on the basis of clinical, radiologic, and histopathologic findings. Immunohistochemical analyses included staining with Ki-67, p53, epidermal growth factor receptor (EGFR), cytokeratin (CK) 8, CK14, CK17, and CK18. Cell immunopositivity was evaluated for the entire epithelium. The criteria for Ki-67 and p53 positivity were dense and/or faint nuclear staining, and cells were considered EGFR-positive if they exhibited membrane staining and/or cytoplasm staining. For the cytokeratins, cells exhibiting cytoplasm staining were considered positive. Five representative fields of each lesion were selected and identified in each of the Ki-67- and p53-stained slides. We found a statistically significant difference in the ratio of Ki-67-positive cells in the entire layer between the keratocystic odontogenic tumors and both the dentigerous cysts and the radicular cysts. A statistically significant difference was observed in the ratio of p53-positive cells between the keratocystic odontogenic tumors and the radicular cysts. Cytokeratins proved to be useful in differentiating radicular cysts from other types of cystic jaw lesions because of their CK8-positive and CK17-negative immunolabeling.

    Topics: Biomarkers; Biopsy; Cell Proliferation; Cytoplasm; Dentigerous Cyst; Diagnosis, Differential; ErbB Receptors; Humans; Immunohistochemistry; Jaw; Jaw Cysts; Keratins; Ki-67 Antigen; Odontogenic Cysts; Odontogenic Tumors; Radicular Cyst; Retrospective Studies; Tumor Suppressor Protein p53

2017
Decompression as a treatment for odontogenic cystic lesions of the jaw.
    Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 2014, Volume: 72, Issue:2

    To evaluate the effectiveness of decompression as the primary treatment of odontogenic cystic lesions of the jaw involving factors that affect relative shrinking speed and bone regeneration.. A total of 32 patients with odontogenic cystic lesions of the jaw underwent decompression with customized thermoplastic resin stents. Clinical examinations and pre- and postdecompression panoramic radiographs were analyzed.. The mean relative speed of shrinkage of radicular cysts (RCs; 3.37 cm(2)/month) was faster than those of keratocystic odontogenic tumors (KCOTs; 2.87 cm(2)/month) and unicystic ameloblastomas (UABs; 2.71 cm(2)/month). The relative shrinking size increased linearly in a time-dependent manner for KCOTs (r = 0.849, P < .001), RCs (r = 0.681, P = .319), and UABs (r = 0.146, P = .730); a similar relation was detected between the primary radiolucent area of cystic lesions before decompression and relative shrinking speed after decompression in KCOTs (r = 0.481, P = .032), RCs (r = 0.260, P = .673), and UABs (r = 0.370, P = .366), but patient age did not affect the relative speed of shrinkage (P > .05). Furthermore, the increase in bone density was more significant in RCs than in KCOTs (P = .026) and UABs (P = .012) after decompression.. Decompression was effective in reducing odontogenic cystic lesions of the jaw and increasing bone density. For aggressive lesions, secondary definitive surgery was necessary.

    Topics: Adult; Ameloblastoma; Bone Density; Decompression, Surgical; Dentigerous Cyst; Female; Humans; Jaw Neoplasms; Keratins; Male; Odontogenic Cysts; Radicular Cyst; Stents

2014
The expression of cytokeratin in keratocystic odontogenic tumor, orthokeratinized odontogenic cyst, dentigerous cyst, radicular cyst and dermoid cyst.
    Medical molecular morphology, 2014, Volume: 47, Issue:3

    The epithelial lining of odontogenic keratocysts exhibits either parakeratosis or orthokeratosis. In 2005, the WHO classified odontogenic keratocysts with parakeratosis as keratocystic odontogenic tumors (KCOT). Odontogenic keratocysts with orthokeratosis were not classified as odontogenic tumors, but instead referred to as orthokeratinized odontogenic cysts (OOC). To clarify the difference between these two lesions, we investigated their biological characteristics using immunohistochemical studies for cytokeratins (CK) in KCOT and OOC as well as in dentigerous cysts (DC), radicular cysts (RC) and dermoid cysts (DMC). We examined twenty-five cases of KCOT, fifteen cases each of OOC, DC and RC, and ten cases of DMC. We studied the immunohistochemical expression of CK10, 13, 17 and 19. To evaluate the immunohistochemical staining pattern, we divided the epithelial lining of the lesions into three layers (surface layer: su, spinous layer: sp, basal layer: ba). For CK10, most OOC and DMC specimens of su and sp were positive. For CK13 and 19, most KCOT, DC and RC specimens of su and sp were positive. For CK17, most KCOT specimens of su and sp were positive. The percentages of total CK expression of su and sp, and ba of CK19 differed significantly between the lesions (P < 0.001). These results support the hypothesis that OOC originate from not the odontogenic apparatus, but the oral epithelial component.

    Topics: Antibodies, Monoclonal; Dentigerous Cyst; Dermoid Cyst; Epithelium; Humans; Immunohistochemistry; Keratins; Odontogenic Cysts; Odontogenic Tumors; Radicular Cyst

2014
Diagnostic dilemma of unicystic ameloblastoma: novel parameters to differentiate unicystic ameloblastoma from common odontogenic cysts.
    Journal of investigative and clinical dentistry, 2014, Volume: 5, Issue:3

    Diagnostic criteria that have been specified for unicystic ameloblastomas (UAs) are not always helpful to differentiate these cystic tumors from common odontogenic cysts. The aim of this study therefore was to identify additional histopathological features (other than the features considered for the diagnosis of UA at present) that would be helpful to differentiate UA from odontogenic cysts.. One hundred histopathologically confirmed unicystic ameloblastomas and 20 cases each of radicular, inflamed dentigerous and non-inflamed dentigerous cysts were selected. Histopathological features of the UAs that are not used as diagnostic criteria at present were identified.. Hyperplastic arcading epithelial proliferations with stellate-reticulum-like and vacuolated cells were always seen associated with inflammation in odontogenic cysts, while in UA plexiform-like areas were also seen without inflammation (P < 0.001). In addition, a spiky rete pattern was observed in non-inflamed UA while this pattern was observed only in inflamed odontogenic cysts. Furthermore, spiky retes together with subepithelial hyalinization were usually observed in UAs while only subepithelial hyalinization was observed in non-inflamed dentigerous cysts.. Combinations of histopathological features were identified to differentiate non-inflamed UA from common odontogenic cysts. However, presence of inflammatory changes in UA precludes the use of features identified in the present study for diagnostic purposes.

    Topics: Ameloblastoma; Ameloblasts; Connective Tissue; Dentigerous Cyst; Diagnosis, Differential; Epithelial Cells; Epithelium; Female; Humans; Hyalin; Hyperplasia; Inflammation; Keratins; Male; Radicular Cyst; Vacuoles; Young Adult

2014
Expression of basement membrane components in odontogenic cysts.
    Oral diseases, 2006, Volume: 12, Issue:3

    To compare the expression of basement membrane components (BMCs), including laminins 1 and 5, collagen type IV, and fibronectin in odontogenic keratocysts (OKCs) with dentigerous cysts (DCs) and radicular cysts (RCs).. Basement membrane components were analysed in 20 OKCs, 20 DCs and 20 RCs using an immunohistochemical technique.. Odontogenic keratocysts, DCs and RCs showed positive reaction to all BMCs studied, with different distributions and intensity. OKCs showed continuous linear deposits for laminins 1 and 5 but two staining patterns (continuous and discontinuous) for collagen type IV and fibronectin. DCs exhibited continuous linear deposits for laminins 1 and 5 and collagen type IV but a discontinuous linear deposit for fibronectin. RCs displayed similar results to DCs for laminin 1, collagen type IV and fibronectin. Laminin 5 in RCs had two staining patterns. Constant results in all cysts were strong intensity for laminin 1 and moderate intensity for laminin 5.. Substantial differences in the expression of BMCs among studied cysts were not observed, suggesting that the separation of the epithelial lining in OKCs is not associated with the existence of these proteins.

    Topics: Basement Membrane; Collagen Type IV; Connective Tissue; Dentigerous Cyst; Epithelium; Fibronectins; Humans; Keratins; Laminin; Odontogenic Cysts; Radicular Cyst

2006
Cytokeratin expression patterns for distinction of odontogenic keratocysts from dentigerous and radicular cysts.
    Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 2005, Volume: 34, Issue:9

    The clinical outcome of treatment of odontogenic cysts differs depending on separate entities. Particular clinical relevance must be attached to the distinction between odontogenic keratocysts, which have an evident tendency to recur, and other odontogenic cysts. The aim of this study was to evaluate cytokeratin (CK) expression patterns as an additional tool for characterization of different cysts as the histomorphologic appearance often is not decisive.. Thirty cases of dentigerous and radicular cysts respectively as well as 15 cases of odontogenic keratocysts were considered. Expression of CK 5/6, 7, 10, 13, 17, 19 and 20 was determined in addition to Ki-67 immunohistochemically.. Expression of CK 17 was discernible in 93.3% of the odontogenic keratocysts, but only in 35.0% of dentigerous and radicular cysts under study (P < 0.001). CK 19 could be detected in 48.3% of dentigerous and radicular cysts, whereas odontogenic keratocysts were completely negative (P < 0.002).. Immunohistochemical detection of CK 17 and 19 seems to be a valuable additional parameter distinguishing between odontogenic keratocysts and other odontogenic--especially dentigerous--cysts which clinically are likely the most significant differential diagnoses in this context. J Oral Pathol Med (2005) 34: 558-64.

    Topics: Adolescent; Adult; Aged; Biomarkers; Child; Dentigerous Cyst; Diagnosis, Differential; Female; Humans; Immunohistochemistry; Intermediate Filament Proteins; Keratin-20; Keratin-7; Keratins; Male; Middle Aged; Odontogenic Cysts; Radicular Cyst

2005
Ameloblastic fibroma: report of a case.
    Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 2002, Volume: 60, Issue:2

    Topics: Child, Preschool; Dentigerous Cyst; Diagnosis, Differential; Female; Follow-Up Studies; Humans; Keratins; Mandibular Diseases; Mandibular Neoplasms; Mesoderm; Odontogenic Tumors; Vimentin

2002
Cytokeratin expression in the odontogenic keratocyst.
    Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 2000, Volume: 58, Issue:8

    This study attempted to identify differential cytokeratin expression in cystic jaw lesions using immunohistochemical staining.. The charts from selected patients treated between 1983 and 1994 for jaw cysts were evaluated. Twenty-four paraffinized specimens were selected randomly for investigation with 5 immunohistochemical stains. The 4 diagnostic categories included ameloblastoma, dentigerous cyst, odontogenic keratocyst (OKC), and recurrent odontogenic keratocyst in patients with nevoid basal cell carcinoma (NBCC) syndrome. The 5 immunohistochemical stains included antibodies to cytokeratins 13, 17, and 18; CAM 5.2; AE 1/3; and carcinoembryonic antigen (CEA).. Differential staining of OKCs from patients with and without NBCC syndrome was found only with the antibody to cytokeratin 17. Furthermore, staining of OKCs in syndromic patients appeared to be stronger and more uniform than in nonsyndromic patients.. These findings suggest that immunohistochemical staining for cytokeratin 17 may aid in the diagnosis of OKCs and may be used to further subdivide these lesions based on the presence or absence of NBCC syndrome.

    Topics: Ameloblastoma; Antibodies, Monoclonal; Basal Cell Nevus Syndrome; Dentigerous Cyst; Diagnosis, Differential; Humans; Immunoenzyme Techniques; Jaw Neoplasms; Keratins; Odontogenic Cysts; Random Allocation; Sampling Studies; Staining and Labeling

2000
Differentiation of odontogenic keratocysts from nonkeratinizing cysts by use of fine-needle aspiration biopsy and cytokeratin-10 staining.
    Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 2000, Volume: 58, Issue:9

    In this study, the efficacy of fine-needle aspiration biopsy (FNAB) and cytokeratin 10 immunocytochemical staining to differentiate odontogenic keratocysts (OKC) from dentigerous and other nonkeratinizing cysts was evaluated.. This was a prospective study of 18 FNABs of odontogenic cystic lesions performed at the Massachusetts General Hospital between 1995 and 1998. A consistent and standardized technique was used to obtain the cytologic material. Immunocytochemistry was performed on destained smears by using a monoclonal antibody against cytokeratin 10. Identical immunohistochemical methods were applied to the final surgical specimen, and results were compared.. Cells of 10 of 18 FNABs showed a markedly positive immunoreaction to anti-cytokeratin 10, supporting a diagnosis of OKC. In all 10 cases, the diagnosis was confirmed by histology. Six of 18 cases showed an absence of staining and were interpreted as anti-cytokeratin 10 negative. In the 2 remaining cases, there were occasional squamous cells on the smear with weak anti-cytokeratin 10 uptake. The overall pattern was negative, and these were interpreted as nonkeratinizing cysts. In all 8 of these cases, the diagnosis of OKC was excluded based on the immunocytochemistry, and the final histologic diagnoses were: dentigerous cyst (n = 4) and radicular cyst (n = 4).. The combination of FNAB with immunocytochemical determination of cytokeratin 10 expression by sampled epithelial cells was 100% accurate in distinguishing an OKC from a nonkeratinizing odontogenic cyst in this series. The technique allows for early diagnosis and rational surgical planning.

    Topics: Antibodies, Monoclonal; Biopsy, Needle; Dentigerous Cyst; Diagnosis, Differential; Humans; Immunoenzyme Techniques; Keratins; Odontogenic Cysts; Prospective Studies; Radicular Cyst; Reproducibility of Results

2000
Increased elafin expression in cystic, dysplastic and neoplastic oral tissues.
    Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 1996, Volume: 25, Issue:3

    Expression of human leukocyte elastase inhibitor, elafin, otherwise known as skin-derived antileukoproteinase inhibitor (SKALP), was investigated in normal and abnormal oral tissues using a specific anti-SKALP rabbit antiserum. Weak staining was observed in keratinizing epithelia of normal oral mucosa but not in non-keratinizing mucosa. Increased expression was also observed in the suprabasal layers of dysplastic oral epithelia and in well-differentiated squamous cell carcinoma, but not in basal cell carcinoma. A uniform strong expression was observed in all supra-basal layers of odontogenic keratocyst epithelia, except in regions where inflammatory infiltrate was adjacent to keratocyst epithelia. In contrast, elafin expression in a small number of dentigerous cysts and ameloblastomas was more patchy. The increased levels of elafin in keratocyst epithelia and dysplastic tissue may be a cellular homoeostatic response to generate a protective barrier preventing proteolytic degradation of underlying elastic tissue.

    Topics: Ameloblastoma; Animals; Carcinoma, Basal Cell; Carcinoma, Squamous Cell; Dental Sac; Dentigerous Cyst; Elastic Tissue; Epithelium; Gene Expression Regulation; Gene Expression Regulation, Neoplastic; Gingiva; Homeostasis; Humans; Keratins; Mouth Mucosa; Mouth Neoplasms; Odontogenic Cysts; Peptide Hydrolases; Proteinase Inhibitory Proteins, Secretory; Proteins; Rabbits; Serine Proteinase Inhibitors

1996
Jaw cysts with orthokeratinization: analysis of 12 cases.
    Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 1993, Volume: 22, Issue:1

    The clinico-pathologic, immunohistochemical and radiological features of 12 jaw cysts with a prominent orthokeratinized epithelial lining were studied and compared with those of typical odontogenic keratocysts and dentigerous cysts. They differed significantly from odontogenic keratocysts in terms of biologic behavior and histopathologic findings. Although immunohistochemical staining of the epithelial linings for cytokeratins, EMA, CEA and involucrin has not shed any light on the histogenesis of these lesions, staining patterns for these markers were significantly different from those of odontogenic keratocysts and non-keratinized dentigerous cysts. Radiologically, nine cases appeared as dentigerous cysts; two cases, one with sebaceous differentiation, as non-dentigerous unilocular cysts, and the remaining one was exceptional as it showed multiple epidermal cysts with prominent dermal appendages histologically. It is suggested that most of the orthokeratinized jaw cysts may belong to clinico-pathological entities different from odontogenic keratocysts with the majority representing dentigerous cysts with orthokeratinization. The possibility of the existence of rare central dermoid or epidermoid cysts is also to be considered.

    Topics: Adolescent; Adult; Child; Child, Preschool; Dentigerous Cyst; Dermoid Cyst; Female; Humans; Immunohistochemistry; Jaw Cysts; Keratins; Keratosis; Male; Odontogenic Cysts

1993
The nature of ghost cells in calcifying odontogenic cyst: an immunohistochemical study.
    Annals of dentistry, 1993,Summer, Volume: 52, Issue:1

    Two solid and two cystic forms of calcifying odontogenic cysts were stained immunohistochemically to study keratin, carcinoembryonic antigen, epithelial membrane antigen, and S-100 protein expression in ghost cells. The patterns of immunoreactivity were compared with those of dentigerous cysts, odontogenic keratocysts, ameloblastomas, calcifying epitheliomas of Malherbe, and control samples. Immunostaining patterns of calcifying odontogenic cysts were found to be similar to the odontogenic lesions and different from calcifying epithelioma. It is concluded that ghost cells are "keratinizing" odontogenic cells showing aberrant differentiation. These cells should not be regarded as metaplastic. The similarity of the immunostaining patterns of cystic and solid calcifying odontogenic cysts supports the view that these lesions are two morphologic variants of the same entity.

    Topics: Ameloblastoma; Antigens, Neoplasm; Biomarkers, Tumor; Carcinoembryonic Antigen; Dentigerous Cyst; Humans; Immunohistochemistry; Keratins; Membrane Glycoproteins; Mouth Mucosa; Mucin-1; Odontogenic Cysts; Odontogenic Tumors; S100 Proteins; Skin; Skin Neoplasms

1993
Scanning electron microscopy of odontogenic cyst epithelium.
    The Journal of the Dental Association of South Africa = Die Tydskrif van die Tandheelkundige Vereniging van Suid-Afrika, 1992, Volume: 47, Issue:5

    The epithelial lining of 8 odontogenic keratocysts (OKC), 9 radicular (RC) and 3 dentigerous cysts (DC) were examined in SEM in order to study the ultrastructural surface topography of the lumenal surface cells. The orthokeratinized OKC showed a reticular network of intercommunicating microridges surrounding micropits giving a honeycombed appearance to the entire surface. The deep surface of these cells was covered by a complementary array of short stubby microvilli. This pattern was identical to that described for oral epithelium in areas of masticatory mucosa. The parakeratinized OKC showed a complex pattern of microplications (MP) on both upper and deep cell surfaces. The non-keratinized linings of RC and DC revealed a similar MP pattern but of a less complex nature. The MP pattern of cells from para- and non-keratinized cysts was identical to that described for oral epithelial cells from lining mucosa. The surface ultrastructure of ciliated, mucus and brush cells occurring in RC was found to be indistinguishable from that described in the mammalian respiratory tract. The MP pattern forms part of the cellular interdigitation mechanisms in stratified squamous epithelium. Differences in the ultrastructural configuration are related to the type of epithelium in terms of keratinization rather than to protective functions.

    Topics: Adolescent; Adult; Aged; Dentigerous Cyst; Epithelium; Female; Humans; Keratins; Male; Microscopy, Electron, Scanning; Middle Aged; Odontogenic Cysts; Radicular Cyst

1992
Lectin histochemistry of cystic jaw lesions: an aid for differential diagnosis between cystic ameloblastoma and odontogenic cysts.
    Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 1991, Volume: 20, Issue:3

    The binding sites for Ulex europaeus agglutinin I (UEA-I), Bandeirea simplicifolia agglutinin I (BSA-I), and peanut agglutinin (PNA) were comparatively examined in the surgical materials from 41 cases of cystic and solid ameloblastomas and 42 cases of non-neoplastic odontogenic cysts including dentigerous cyst, odontogenic keratocyst, and radicular cyst. In non-neoplastic cysts, most of epithelial lining layers gave positive binding with UEA-I and BSA-I. However, no positive reactions were obtained for these two lectins in the epithelial components of ameloblastoma, except for limited UEA-I binding to markedly keratinized tumor cells in four cases. PNA binding was irregular and did not make any clear distinction between ameloblastomas and cysts. The results suggest that the lectin staining for UEA-I and BSA-I is a useful histologic aid for differential diagnosis between cystic ameloblastoma and non-neoplastic jaw cysts.

    Topics: Ameloblastoma; Biomarkers, Tumor; Dentigerous Cyst; Diagnosis, Differential; Endothelium, Vascular; Epithelium; Humans; Immunoenzyme Techniques; Jaw Neoplasms; Keratins; Lectins; Odontogenic Cysts; Peanut Agglutinin; Plant Lectins; Radicular Cyst

1991
An asymptomatic radiolucency of the posterior maxilla.
    Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 1991, Volume: 49, Issue:10

    Topics: Ameloblastoma; Dentigerous Cyst; Diagnosis, Differential; Female; Humans; Keratins; Middle Aged; Odontogenic Cysts; Odontogenic Tumors; Radicular Cyst; Radiography

1991
Diagnostic importance of cytokeratin expression in linings of odontogenic cysts.
    Journal of clinical pathology, 1990, Volume: 43, Issue:1

    Topics: Dentigerous Cyst; Humans; Keratins; Odontogenic Cysts

1990
[Expression of certain cytokeratins in the epithelium of dentigerous and primordial cysts].
    Journal de biologie buccale, 1990, Volume: 18, Issue:1

    The histopathologic diagnosis of odontogenic cysts is based mainly on the morphological nature of the epithelial lining of the cyst. A standard immunocytochemical method based on anticytokeratin monoclonal antibodies was used for the diagnosis of dentigerous and primordial cysts: 12 odontogenic cysts were diagnosed on clinical, radiological and pathological criteria in 9 dentigerous cysts and 3 primordial cysts. The anticytokeratin antibodies used in this study were KL1 (Immunotech, France) and AE1, AE2, AE3 and AE8 (ICN-Miles, France). The anticytokeratin antibodies used stained only the epithelial cells confirming their accuracy. The KL1 antibodies stained homogeneously the various epithelial cells. This positive reaction was not modified by the various fixation methods used. Some reactions observed with AE antibodies seemed to be modified by Bouin's fixative. The staining homogeneity of the primordial cysts and the staining heterogeneity of the dentigerous cysts seemed to be related to morphological aspects of their respective epithelia. The epithelial reactions in these 2 types of cysts towards inflammation were different.

    Topics: Antibodies, Monoclonal; Cell Division; Dentigerous Cyst; Diagnosis, Differential; Epithelium; Fixatives; Humans; Immunohistochemistry; Keratinocytes; Keratins; Maxillary Diseases; Odontogenic Cysts

1990
Immunohistochemical study of jaw cysts: different existence of keratins in odontogenic and non-odontogenic epithelial linings.
    Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 1990, Volume: 19, Issue:7

    Keratins and secretory component (SC) were immunohistochemically examined in fresh tissue samples from 45 odontogenic and 35 non-odontogenic cysts. Lining epithelia of almost all cases contained keratins which reacted with polyclonal antibodies (Dako, Bio-Science), and no difference could be found between the two groups of lesions. By staining with two monoclonal antibodies against keratins, i.e., RGE53 (Bio-Science) and RKSE60 (Bio-Science), it was revealed that the epithelia of non-odontogenic cysts, which were columnar epithelium in most cases, had fully and positively reacted with RGE53, while none of the cases was positive for RKSE60. In contrast, the squamous linings of odontogenic cysts except for two cases did not react with RGE53, and few cases possessed RKSE60-reactive keratin. SC was also contradictory. All non-odontogenic cysts exhibited SC. Regarding each pair of non-odontogenic and odontogenic cysts covered with RGE53 and SC-positive, and RKSE60-negative squamous epithelium, it seemed reasonable from the staining results to conclude that the squamous linings were metaplastic from the columnar epithelium. Based on the results, concomitant examinations of SC with keratins will be helpful in deciding the epithelial derivation of jaw cysts.

    Topics: Dentigerous Cyst; Epithelium; Humans; Immunohistochemistry; Jaw Cysts; Keratins; Metaplasia; Molecular Weight; Nonodontogenic Cysts; Odontogenic Cysts; Radicular Cyst; Secretory Component

1990
Cytokeratin expression of the odontogenic epithelia in dental follicles and developmental cysts.
    Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 1989, Volume: 18, Issue:2

    The patterns of cytokeratin expression in the epithelium of 5 dental follicles, 7 dentigerous cysts, 5 odontogenic keratocysts, 3 nasopalatine cysts and an epidermoid cyst have been studies using a panel of monoclonal antibodies. The epithelium of dental follicles and of developmental odontogenic cysts strongly expressed keratins 5 and 19 and showed weaker expression of keratins typical of stratified non-cornified and of simple epithelia. Staining with mAbs against the latter keratins varied with the degree of epithelial differentiation. Nasopalatine cysts strongly expressed simple epithelial keratins and the epidermoid cyst strongly expressed a marker of cornification. Odontogenic cysts thus appear to differ in their pattern of keratin expression from other oral developmental cysts and all derivatives of odontogenic epithelia appear to share similar basic patterns of cytokeratin expression.

    Topics: Antibodies, Monoclonal; Dental Sac; Dentigerous Cyst; Epidermal Cyst; Epithelium; Fluorescent Antibody Technique; Humans; Immunoenzyme Techniques; Keratins; Nonodontogenic Cysts; Odontogenic Cysts; Tooth Germ

1989
Expression of cytokeratin in the epithelium of dentigerous cysts and odontogenic keratocysts: an aid to diagnosis.
    Journal of clinical pathology, 1989, Volume: 42, Issue:7

    Sections of tissue embedded in paraffin wax from 18 selected odontogenic cysts were studied both histologically and immunohistochemically with antibodies to cytokeratins using the indirect peroxidase technique. The cysts were divided on a clinical and histological basis into two equal groups comprising dentigerous cysts and odontogenic keratocysts. It was possible to differentiate the two cyst types in every case by the pattern of staining using the monoclonal antibody LP34 for cytokeratins of intermediate molecular weight. The monoclonal antibody CAM 5.2 for cytokeratins of low molecular weight was not discriminatory. Such a clear distinction may prove useful diagnostically in distinguishing between two cysts of similar appearance but very different behaviour.

    Topics: Dentigerous Cyst; Diagnosis, Differential; Epithelium; Humans; Jaw Diseases; Keratins; Odontogenic Cysts; Recurrence

1989
Mast cells in human odontogenic cysts.
    Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 1989, Volume: 18, Issue:5

    Mast cells have been shown to be present in substantial numbers in both nonkeratinizing and keratinizing odontogenic cysts and could be seen in the connective tissue capsule and the epithelial lining. Within the cyst capsule, mast cells were more prevalent just beneath the epithelium than in deeper areas. This distribution pattern for mast cells is in accord with the histochemical picture for heparin staining in odontogenic cysts. In the non-keratinizing cysts, there appeared to be some trend towards mast cells being associated with increasing inflammation but not in the odontogenic keratocyst. No evidence could be found for distinct mast cell subpopulations in odontogenic cysts. The presence of mast cells in odontogenic cyst could contribute to their pathogenesis in several ways.

    Topics: Cell Count; Connective Tissue; Dentigerous Cyst; Epithelium; Humans; Keratins; Mast Cells; Odontogenic Cysts; Radicular Cyst; Staining and Labeling

1989
[Odontogenic keratocyst--a retrospective study of its clinical picture, radiology, pathohistology and therapy].
    Schweizerische Monatsschrift fur Zahnmedizin = Revue mensuelle suisse d'odonto-stomatologie = Rivista mensile svizzera di odontologia e stomatologia, 1986, Volume: 96, Issue:4

    Topics: Adult; Aged; Dentigerous Cyst; Female; Follow-Up Studies; Humans; Jaw Diseases; Keratins; Male; Middle Aged; Odontogenic Cysts; Osteolysis; Radicular Cyst; Radiography; Recurrence; Retrospective Studies

1986
Distribution of type 1 and 2 blood group chains in normal and pathological odontogenic epithelium defined by monoclonal antibodies specific for Lea and H type 2.
    Acta pathologica, microbiologica, et immunologica Scandinavica. Section A, Pathology, 1985, Volume: 93, Issue:5

    This study describes the distribution of type 1 and type 2 blood group carbohydrate chains in human normal and pathological odontogenic epithelia and in epithelia of human oral mucosa. Odontogenic epithelium was examined from 12 fetal tooth germs, 25 ameloblastomas, 13 odontogenic keratocysts, 13 follicular cysts and 13 radicular cysts. Oral mucosal epithelia was studied from 12 fetuses and 10 adults. Cell surface carbohydrates were detected using antibodies with reactivity for the blood group antigens A, B, type 1 chain Lea and type 2 chain H by an immunofluorescence technique. The expression of Lea and H type 2 chain in fetal palatal epithelium and only H type 2 chain in adult palatal epithelium suggests that a change in synthesis of blood group chains occurs during development. Type 2 blood group chains (antigen H) were found in fetal tooth germs, type 1 (Lea) in ameloblastomas and both type 1 and type 2 in odontogenic cysts. These results indicate that a modulation in synthesis of blood group carbohydrates has occurred in ameloblastomas and odontogenic cysts as compared with the cells from which the lesions presumably are developed. It is suggested that ameloblastomas may be distinguished from odontogenic cysts by the inability of ameloblastomas to synthesize type 2 blood group chains and antigens A and B.

    Topics: ABO Blood-Group System; Adolescent; Adult; Aged; Ameloblastoma; Antibodies, Monoclonal; Dentigerous Cyst; Epithelium; Female; Fetus; H-2 Antigens; Humans; Keratins; Lewis Blood Group Antigens; Male; Middle Aged; Mouth Mucosa; Odontogenic Cysts; Odontogenic Tumors; Radicular Cyst; Tooth Germ

1985
The odontogenic keratocyst: orthokeratinized variant.
    Oral surgery, oral medicine, and oral pathology, 1981, Volume: 51, Issue:6

    The histopathologic and clinical features of sixty cases of orthokeratinized odontogenic cysts were compared with those of odontogenic keratocysts (typically parakeratinized). According to the results of this study, the orthokeratinized odontogenic cyst appears to be a distinct clinicopathologic entity. This cyst is histologically characterized by a thin, uniform, epithelial lining with orthokeratinization and a subjacent granular cell layer. The basal cells are usually cuboidal or flattened. Clinically, the orthokeratinized cyst is a single cyst, shows a predilection for males, and is most often found in the second to the fifth decade, it appears most commonly as a dentigerous cyst in the posterior mandible. The orthokeratinized cyst shows little clinical aggressiveness. Follow-up of twenty-four patients revealed only one recurrence; of nineteen patients evaluated, none had features of the basal cell nevus-bifid rib syndrome. It is suggested that this cyst be called odontogenic keratocyst, orthokeratinized variant.

    Topics: Adolescent; Adult; Aged; Connective Tissue; Dentigerous Cyst; Epithelium; Female; Humans; Jaw Diseases; Keratins; Male; Mandibular Diseases; Middle Aged; Odontogenic Cysts

1981
Respiratory epithelium as an integral part of an odontogenic keratocyst: report of case.
    Journal of oral surgery (American Dental Association : 1965), 1980, Volume: 38, Issue:6

    A case is reported in which the odontogenic keratocyst that appeared to be a dentigerous cyst associated with an impacted mandibular third molar was found histologically to demonstrate the keratinizing stratified squamous epithelium characteristic of these lesions in direct continuity with ciliated columnar epithelium.

    Topics: Adult; Cilia; Dentigerous Cyst; Epithelium; Humans; Keratins; Male; Mandibular Diseases

1980
[Keratinized dentigerous cyst with evolution to ameloblastoma].
    Ars curandi em odontologia, 1979, Volume: 6, Issue:3

    Topics: Adolescent; Ameloblastoma; Dentigerous Cyst; Female; Humans; Keratins; Mandibular Neoplasms

1979
The odontogenic keratocyst. A clinicopathologic study of 312 cases. Part II. Histologic features.
    Oral surgery, oral medicine, and oral pathology, 1977, Volume: 43, Issue:2

    The histopathologic features of 312 odontogenic keratocysts are described. These features are discussed in relation to their pathogenesis and behavioral characteristics, including recurrence factors and neoplastic potential. In addition, a discussion and comparison of the histologic features found in solitary keratocysts, keratocysts associated with the basal-cell nevus--bifid rib syndrome, multiple keratocysts in patients with no other syndrome manifestations, and recurrent kerocysts is presented.

    Topics: Collagen; Connective Tissue; Dentigerous Cyst; Epithelium; Humans; Jaw Neoplasms; Keratins; Mitosis; Neoplasms, Multiple Primary; Odontogenic Cysts; Tooth Root

1977
Radiologic appearances of oral and perioral cysts.
    Ontario dentist, 1975, Volume: 52, Issue:11

    Topics: Bone Cysts; Cysts; Dentigerous Cyst; Humans; Keratins; Mouth Diseases; Nonodontogenic Cysts; Odontogenic Cysts; Radicular Cyst; Radiography

1975
A note on the origin of keratocysts of the jaws.
    International journal of oral surgery, 1973, Volume: 2, Issue:2

    Topics: Adult; Aged; Animals; Cysts; Dentigerous Cyst; Female; Fetus; Haplorhini; Humans; Jaw Diseases; Jaw Neoplasms; Keratins; Macaca; Male; Mandible; Middle Aged; Molar; Mouth Mucosa; Nonodontogenic Cysts; Pregnancy

1973
Proteins in fluid from non-keratinizing jaw cysts. 1. Separation patterns on cellulose acetate membranes and percentage distribution of the electrophoretic fractions.
    Journal of oral pathology, 1973, Volume: 2, Issue:2

    Topics: Acetates; Adult; Animals; Biopsy, Needle; Blood Donors; Blood Proteins; Cattle; Cellulose; Child; Dentigerous Cyst; Electrophoresis; Humans; Hyaluronoglucosaminidase; Immunoglobulins; Jaw Neoplasms; Keratins; Nonodontogenic Cysts; Periodontal Cyst; Proteins; Viscosity

1973
Odontogenic cysts. A review and a clinicopathological study of 368 odontogenic cysts.
    Australian dental journal, 1973, Volume: 18, Issue:4

    Topics: Adult; Age Factors; Bicuspid; Biopsy; Cuspid; Dentigerous Cyst; Female; Humans; Incisor; Keratins; Male; Mandibular Neoplasms; Maxillary Neoplasms; Molar; Odontogenic Cysts; Prospective Studies; Retrospective Studies; Sex Factors

1973
Metaplasia and degeneration in odontogenic cysts in man.
    Journal of oral pathology, 1972, Volume: 1, Issue:4

    Topics: Adolescent; Adult; Age Factors; Aged; Child; Dentigerous Cyst; Epithelial Cells; Epithelium; Female; Humans; Hyalin; Keratins; Male; Mandible; Maxilla; Metaplasia; Middle Aged; Mucus; Odontogenic Cysts; Periodontal Cyst; Staining and Labeling

1972
[The proof of some enzymes in jaw cysts].
    Ceskoslovenska stomatologie, 1972, Volume: 72, Issue:3

    Topics: Acid Phosphatase; Adolescent; Adult; Aged; Alkaline Phosphatase; Child; Cholesterol; Dentigerous Cyst; Enzymes; Female; Humans; Jaw Diseases; Keratins; Male; Middle Aged; Odontogenic Cysts; Radicular Cyst

1972
Keratocysts of the jaws.
    Journal of oral surgery (American Dental Association : 1965), 1972, Volume: 30, Issue:11

    Topics: Adolescent; Adult; Child; Dentigerous Cyst; Humans; Jaw Diseases; Jaw Neoplasms; Keratins; Middle Aged; Nonodontogenic Cysts; Odontogenic Cysts; Radicular Cyst; Radiography; Recurrence

1972
Cystic lesions of the jaws.
    Annals of the Royal College of Surgeons of England, 1971, Volume: 49, Issue:6

    Topics: Bone Cysts; Dentigerous Cyst; Humans; Jaw Diseases; Jaw Neoplasms; Keratins; Lymphatic System; Methods; Nonodontogenic Cysts; Odontogenic Cysts; Osmosis; Recurrence

1971
The pathogenesis of the odontogenic keratocyst.
    Proceedings Of The International Academy Of Oral Pathology, 1969

    Topics: Dentigerous Cyst; Dentistry; Keratins; Odontogenic Cysts

1969
STUDIES ON ODONTOGENIC CYST EPITHELIUM. 2. CLINICAL AND ROENTGENOLOGIC ASPECTS OF ODONTOGENIC KERATOCYSTS.
    Acta pathologica et microbiologica Scandinavica, 1963, Volume: 58

    Topics: Dentigerous Cyst; Epithelium; Histological Techniques; Humans; Keratins; Odontogenic Cysts; Odontogenic Tumors; Radiography, Dental

1963