bromochloroacetic-acid and Corneal-Ulcer

bromochloroacetic-acid has been researched along with Corneal-Ulcer* in 2 studies

Other Studies

2 other study(ies) available for bromochloroacetic-acid and Corneal-Ulcer

Article	Year
Corneal epithelial-specific cytokeratin 3 is an autoantigen in Wegener's granulomatosis-associated peripheral ulcerative keratitis. Investigative ophthalmology & visual science, 1999, Volume: 40, Issue:9 In a previous investigation it was demonstrated that circulating antibodies to a 66-kDa corneal epithelial antigen (BCEA-A) are associated with peripheral ulcerative keratitis (PUK) in patients with Wegener's granulomatosis (WG). The aim of this study was to identify BCEA-A.. The 66-kDa antigen was purified from a bovine corneal epithelial protein extract, using DE52 ion exchange chromatography. Purified protein was used to raise rabbit polyclonal antibodies. These antibodies were used to screen a bovine corneal epithelial cDNA expression library. Positive clones were purified and sequenced. Clones were identified by DNA sequence homology searches of the GenBank DNA database.. A cDNA clone that demonstrated strong binding to both the rabbit polyclonal antibody and patient sera, showed 85% homology to rabbit cytokeratin 3 (K3). K3 is a basic cytokeratin specific to corneal epithelium. No bovine DNA sequence for K3 is available. However, bovine K3 is larger than rabbit K3, with a molecular weight of 66 kDa. Immunofluorescence using both patient sera and the rabbit antibody demonstrated a cytoplasmic binding pattern on human corneal epithelium.. This evidence suggests that the 66-kDa autoantigen (BCEA-A) associated with PUK in WG is cytokeratin 3, and this may form the basis of a diagnostic/prognostic test. Topics: Amino Acid Sequence; Animals; Autoantigens; Cattle; Chromatography, Ion Exchange; Corneal Ulcer; DNA Primers; Electrophoresis, Polyacrylamide Gel; Epithelium, Corneal; Fluorescent Antibody Technique, Indirect; Granulomatosis with Polyangiitis; Humans; Keratins; Molecular Sequence Data; Molecular Weight; Rabbits; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid	1999
Characterization and inhibition of corneal collagenase. Investigative ophthalmology, 1971, Volume: 10, Issue:7 Topics: Antimetabolites; Blood; Calcium; Carbon Isotopes; Chelating Agents; Chemical Phenomena; Chemistry; Collagen; Connective Tissue Cells; Cornea; Corneal Ulcer; Culture Techniques; Cysteine; Edetic Acid; Glycosaminoglycans; Histocytochemistry; Humans; Hydrogen-Ion Concentration; Iodoacetates; Keratins; Microbial Collagenase; Microscopy, Electron; Sodium; Sulfates; Sulfhydryl Compounds; Viscosity	1971

Article

Year

Corneal epithelial-specific cytokeratin 3 is an autoantigen in Wegener's granulomatosis-associated peripheral ulcerative keratitis.

Investigative ophthalmology & visual science, 1999, Volume: 40, Issue:9

In a previous investigation it was demonstrated that circulating antibodies to a 66-kDa corneal epithelial antigen (BCEA-A) are associated with peripheral ulcerative keratitis (PUK) in patients with Wegener's granulomatosis (WG). The aim of this study was to identify BCEA-A.. The 66-kDa antigen was purified from a bovine corneal epithelial protein extract, using DE52 ion exchange chromatography. Purified protein was used to raise rabbit polyclonal antibodies. These antibodies were used to screen a bovine corneal epithelial cDNA expression library. Positive clones were purified and sequenced. Clones were identified by DNA sequence homology searches of the GenBank DNA database.. A cDNA clone that demonstrated strong binding to both the rabbit polyclonal antibody and patient sera, showed 85% homology to rabbit cytokeratin 3 (K3). K3 is a basic cytokeratin specific to corneal epithelium. No bovine DNA sequence for K3 is available. However, bovine K3 is larger than rabbit K3, with a molecular weight of 66 kDa. Immunofluorescence using both patient sera and the rabbit antibody demonstrated a cytoplasmic binding pattern on human corneal epithelium.. This evidence suggests that the 66-kDa autoantigen (BCEA-A) associated with PUK in WG is cytokeratin 3, and this may form the basis of a diagnostic/prognostic test.

Topics: Amino Acid Sequence; Animals; Autoantigens; Cattle; Chromatography, Ion Exchange; Corneal Ulcer; DNA Primers; Electrophoresis, Polyacrylamide Gel; Epithelium, Corneal; Fluorescent Antibody Technique, Indirect; Granulomatosis with Polyangiitis; Humans; Keratins; Molecular Sequence Data; Molecular Weight; Rabbits; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid

1999

Characterization and inhibition of corneal collagenase.

Investigative ophthalmology, 1971, Volume: 10, Issue:7

Topics: Antimetabolites; Blood; Calcium; Carbon Isotopes; Chelating Agents; Chemical Phenomena; Chemistry; Collagen; Connective Tissue Cells; Cornea; Corneal Ulcer; Culture Techniques; Cysteine; Edetic Acid; Glycosaminoglycans; Histocytochemistry; Humans; Hydrogen-Ion Concentration; Iodoacetates; Keratins; Microbial Collagenase; Microscopy, Electron; Sodium; Sulfates; Sulfhydryl Compounds; Viscosity

1971