bromochloroacetic-acid and Anal-Gland-Neoplasms

The role of c-KIT receptor in anal sac gland adenocarcinoma (ASGAC) is unclear despite its importance in the development of tumours. In this preliminary study, the expression of c-KIT was investigated in rarely observed canine ASGAC. The potential use of CAM5.2 in distinguishing ASGAC from perianal gland tumours was also evaluated. ASGAC was diagnosed in five out of 25 examined perianal tumours. By immunohistochemistry, cytosolic (abnormal) c-KIT expression was seen in four of the five cases. CAM5.2 immunoreactivity was detected in neoplastic cells of all ASGAC cases examined, whereas it was not evident in any case of perianal gland tumour. The findings suggest that c-KIT expression and its cellular localization may be important in the oncogenesis of ASGAC and CAM5.2 can be used to distinguish between ASGAC and perianal gland tumours.

Topics: Adenocarcinoma; Anal Gland Neoplasms; Anal Sacs; Animals; Biomarkers; Dog Diseases; Dogs; Keratins; Proto-Oncogene Proteins c-kit

Topics: Adenoma, Sweat Gland; Anal Gland Neoplasms; Animals; Condylomata Acuminata; Female; Humans; Keratins; Middle Aged; Neoplasms, Second Primary; Sweat Gland Neoplasms

Three mouse monoclonal anti-human cytokeratin antibodies were made against human sole epidermis. One of these (KA4) was shown to react with a variety of human simple epithelium, including eccrine and apocrine sweat glands and the luminal cells of the breast ducts and lobules, but failed to decorate interfollicular stratified squamous epithelium. This antibody reacted by the immunoblot technic with cytokeratins of Mr values 54, 46, and 40 kdaltons. KA4 reacted strongly with clear cells found in 11% of breast epithelium in clinically uninvolved nipples and with all Paget's cells in four cases of mammary and five cases of extramammary Paget's disease. These findings suggest a common cellular phenotype for Paget's cells and relates them to a population of cells found in breast epithelium.

Topics: Aged; Anal Gland Neoplasms; Animals; Antibodies, Monoclonal; Antigen-Antibody Reactions; Carcinoembryonic Antigen; Carcinoma, Intraductal, Noninfiltrating; Collodion; Electrophoresis, Polyacrylamide Gel; Female; Histocytochemistry; Humans; Keratins; Mice; Mice, Inbred BALB C; Middle Aged; Paget Disease, Extramammary; Paget's Disease, Mammary; Phenotype; Rabbits; S100 Proteins; Vulvar Neoplasms