bromochloroacetic-acid and Adenocarcinoma-of-Lung

为更好地显示肺腺癌中弹力纤维的变化及其与肿瘤细胞的关系，应用全自动免疫组织化学和全自动特殊染色平台对30例肺腺癌组织细胞进行角蛋白（CK）7和弹力纤维双重染色，结果显示在双染切片中CK7免疫组织化学的特异度和灵敏度与单独染色基本没有差异，两者相结合除可以特异性显示胸膜受侵的情况，还观察到多数厚壁血管弹力纤维的增生和血管壁的重构，以及更多CK7和弹力纤维单独染色难以观察到的肿瘤细胞早期浸润血管壁的现象。.

Topics: Adenocarcinoma of Lung; Automation; Biomarkers, Tumor; Diagnosis, Differential; Elastic Tissue; Humans; Keratin-7; Keratins; Lung Neoplasms; Staining and Labeling

Fibroblast growth factor receptor 1 (FGFR1) is frequently amplified in human small-cell lung cancer (SCLC), but its contribution to SCLC and other lung tumors has remained elusive. Here, we assess the tumorigenic capacity of constitutive-active FGFR1 (FGFR1

Topics: Adenocarcinoma of Lung; Animals; Bronchi; Cell Transformation, Neoplastic; Cisplatin; Drug Resistance, Neoplasm; Gene Expression Regulation, Neoplastic; Humans; Integrases; Keratins; Lung Neoplasms; Mice; Mutation; Nasal Cavity; Neurosecretory Systems; Oncogenes; Pulmonary Alveoli; Receptor, Fibroblast Growth Factor, Type 1; Retinoblastoma Protein; Small Cell Lung Carcinoma; Tumor Suppressor Protein p53

Epithelial-mesenchymal transition (EMT) is defined as switching of polarized epithelial cells to a migratory fibroblastoid phenotype. EMT is known to be involved in the progression and metastasis of various cancers. The aim was to evaluate that whether EMT-related proteins' alterations are associated with clinicopathological features and prognosis in lung adenocarcinoma.. The expression of EMT-related proteins including cytokeratin, E-cadherin, TTF-1, β-catenin, vimentin, Snail, Twist, CD44 was evaluated by immunohistochemistry using a tissue array method in the lung adenocarcinoma tissues of 95 patients. In addition, clinicopathological characteristics and survival were compared with the expression of EMT-related proteins.. Loss of epithelial proteins and/or acquisition of the expression of mesenchymal proteins were observed in lung adenocarcinoma. These proteins' alteration was associated with poor cell differentiation and poor patients' outcome, respectively. Subjects were divided into two groups according to the number of EMT-related proteins' alteration. A higher number of EMT-related proteins' alteration was found to be significantly associated with unfavorable outcome. Multivariate analysis showed that a higher number of EMT-related proteins' alteration was independently associated with poor prognosis.. The number of EMT-related proteins' alteration is a significant prognostic marker to predict overall survival in patients with lung adenocarcinoma. The information generated will be valuable for the prognosis of patients with lung adenocarcinoma.. The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1007838329872974.

Topics: Adenocarcinoma; Adenocarcinoma of Lung; Adult; Aged; beta Catenin; Cadherins; Epithelial-Mesenchymal Transition; Female; Gene Expression Regulation, Neoplastic; Humans; Keratins; Lung Neoplasms; Male; Middle Aged; Nuclear Proteins; Prognosis; Thyroid Nuclear Factor 1; Transcription Factors; Vimentin

To establish and characterize a lung adenocarcinoma cell line from a female patient in Xuanwei, Yunnan province.. Surgical specimen of the lung adenocarcinoma was obtained and cultured immediately in RPMI 1640 medium with 10% fetal bovine serum and 10(5) U/L penicillin and 100 mg/L streptomycin. When stable proliferation of the cells was achieved after over 40 passages in culture, the biological features of the cell line were investigated by cell morphology, karyotyping, protein marker expression [cytokeratins (CKs), epithelial membrane antigen (EMA) and CD proteins], growth kinetics, cell cycle phase distribution, mitotic index, colony formation in soft agar, cell invasion and tumorigenicity in Balb/c nude mice.. The established cell line was stably cultured for over 80 passages during a one-year period as an anchorage-dependent monolayer of short spindle, polygonal to epithelioid cells under phase contrast microscope. Microglandular cavities and disordered microfilaments were observed under transmission electron microscope. The growth curve presented in an "S" shape with the cell population doubled every 46.7 hours. The mitotic index was 1.5% and the colony formation rate was 8.3%. The cell cycle distribution included 76.9% in G(0)/G(1), 15.1% in S and 8.0% in G(2)/M. The cell line displayed a hypotriploid karyotype with a mode of 66 chromosomes and a median of 64 chromosomes. The cells expressed CK7, CK8, CK (Pan) and EMA by immunohistochemistry. A high level of cell surface expression of CD13 and CD59 was evident by flow cytometry. The cells were able to penetrate Matrigel in vitro but failed to form a stable xenograft in nude mice.. A new human lung adenocarcinoma cell line, designated as XLA-07, is successfully established from a Xuanwei lung cancer patient.

Topics: Adenocarcinoma; Adenocarcinoma of Lung; Animals; CD13 Antigens; CD59 Antigens; Cell Culture Techniques; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Female; Humans; Karyotyping; Keratins; Lung Neoplasms; Mice; Mice, Inbred BALB C; Mice, Nude; Mucin-1; Neoplasm Transplantation; Polyploidy; Tumor Stem Cell Assay