bombesin--tyr(4)- and Cell-Transformation--Neoplastic

We have introduced a human gastrin-releasing peptide receptor expression vector into an immortalized human bronchial epithelial cell normally unresponsive to the ligand bombesin. Successfully transfected cells express specific binding sites at a density similar to that found at the surface of human lung cancer cells and show an elevation of intracellular calcium concentration in response to bombesin. We found that cellular strains expressing the receptor showed a growth stimulation in response to bombesin in proportion to cell surface receptor density. We conclude that expression of bombesin receptors contributes to the growth potential of human bronchial epithelial cells.

Topics: Animals; Blotting, Northern; Bombesin; Bronchi; Cell Division; Cell Transformation, Neoplastic; Epithelial Cells; Epithelium; Female; Humans; Iodine Radioisotopes; Kinetics; Mice; Mice, Nude; Receptors, Bombesin; RNA, Messenger; Transfection