bombesin--leu(13)-psi-ch2nh-leu(14)- has been researched along with Pheochromocytoma* in 1 studies
1 other study(ies) available for bombesin--leu(13)-psi-ch2nh-leu(14)- and Pheochromocytoma
| Article | Year |
|---|---|
Localization of pp60c-src in growth cone of PC12 cell.
By immunocytochemical and biochemical techniques, we observed the localization and expression of pp60c-src in nerve growth factor (NGF)-treated PC12 cells. Immunostaining of pp60c-src is detected in the neuronal soma and the tips of neurites (growth cones). Immunofluorescence in the neurites is less significant. High-resolution microscopy reveals that the location of pp60c-src in growth cone is in good agreement with the adhesive site of growth cone to the substratum. The pp60c-src kinase activity and the pp60c-src protein level increase 3.1- to 3.5-fold and 2.0-fold during differentiation of PC12 cells, respectively. The pp60c-src levels in the neurite fraction are also higher than those in the neuronal soma fraction. These results support the immunocytochemical finding that pp60c-src is localized in growth cones of differentiated PC12 cells. Furthermore, we discuss the possible role of pp60c-src in growth cone. Topics: Adrenal Gland Neoplasms; Animals; Axons; Bombesin; Cell Differentiation; Fluorescent Antibody Technique; Immunoblotting; Immunohistochemistry; Microscopy, Interference; Nerve Growth Factors; Neurons; Pheochromocytoma; Protein Kinases; Proto-Oncogene Proteins; Proto-Oncogene Proteins pp60(c-src); Rats; Tumor Cells, Cultured | 1988 |