bms-777607 and Tongue-Neoplasms

To investigate the effect of Met kinase inhibitor BMS-777607 on proliferation and apoptosis of tongue squamous cell carcinoma cell line CAL27.. The effect of BMS-777607 on proliferation of CAL27 was detected by MTT method, clone formation assay and EdU cell imaging. Morphological changes of apoptosis of CAL27 cells induced by BMS-777607 were observed by Heochst33342 staining. JC-1 staining was used to detect the changes of mitochondrial membrane potential of CAL27 cells treated with BMS-777607. Western blot was used to detect the effect of BMS-777607 on the expression of proliferation protein Akt, p-Akt and apoptosis-related proteins Bcl-2, Cleaved caspase-3, Bax and Parp in CAL27 cells. The data were analyzed using SPSS 22.0 software package.. BMS-777607 inhibited proliferation and promoted apoptosis of CAL27 cells in a concentration-dependent manner（P＜0.05）. It also inhibited the expression of Bcl-2 and p-Akt and promoted the expression of Bax, Cleaved caspase-3 and Parp protein (P＜0.05).. BMS-777607 can inhibit proliferation and promote apoptosis of CAL27 cells.

Topics: Aminopyridines; Apoptosis; Apoptosis Regulatory Proteins; bcl-2-Associated X Protein; Carcinoma, Squamous Cell; Caspase 3; Cell Line, Tumor; Cell Proliferation; Humans; Poly(ADP-ribose) Polymerase Inhibitors; Proto-Oncogene Proteins c-akt; Proto-Oncogene Proteins c-bcl-2; Pyridones; Tongue; Tongue Neoplasms