bms-777607 and Pre-Eclampsia

Axl encodes the tyrosine-protein kinase receptor, participating in the proliferation and migration of many cells. This study examined the role of Axl in functions of endothelial progenitor cells (EPCs). Axl was detected by RT-PCR and Western blotting in both placentas and EPCs from normal pregnancy and preeclampsia patients. The Axl inhibitor, BMS777-607, was used to inhibit the Axl signalling pathway in EPCs. Cell proliferation, differentiation, migration and adhesion were measured by CCK-8 assay, cell differentiation assay, Transwell assay, and cell adhesion assay, respectively. Results showed the expression levels of Axl mRNA and protein were significantly higher in both placentas and EPCs from preeclampsia patients than from normal pregnancy (P<0.05). After treatment with BMS777-607, proliferation, differentiation, migration and adhesion capability of EPCs were all significantly decreased. Our study suggests Axl may play a role in the function of EPCs, thereby involving in the pathogenesis of preeclampsia.

Topics: Adult; Aminopyridines; Axl Receptor Tyrosine Kinase; Blood Pressure; Case-Control Studies; Cell Adhesion; Cell Differentiation; Cell Movement; Cell Proliferation; Female; Fetal Blood; Gene Expression Regulation; Gestational Age; Human Umbilical Vein Endothelial Cells; Humans; Placenta; Pre-Eclampsia; Pregnancy; Primary Cell Culture; Protein Kinase Inhibitors; Proto-Oncogene Proteins; Pyridones; Receptor Protein-Tyrosine Kinases; RNA, Messenger; Stem Cells