blister and Demyelinating-Diseases

blister has been researched along with Demyelinating-Diseases* in 1 studies

Other Studies

1 other study(ies) available for blister and Demyelinating-Diseases

ArticleYear
A pivotal role of nonmuscle myosin II during microglial activation.
    Experimental neurology, 2014, Volume: 261

    Microglia are resident macrophages in the central nervous system (CNS) and the primary cells that contribute to CNS inflammation in many pathological conditions. Upon any signs of brain damage, microglia become activated and undergo tremendous cellular reorganization to adopt appropriate phenotypes. They migrate to lesion areas, accumulate, phagocytose cells or cellular debris, and produce a large array of inflammatory mediators like cytokines, chemokines, reactive oxygen species, and other mediators. To cope with the extreme cellular rearrangements during activation, microglia have to be highly dynamic. One major component of the cytoskeleton in nonmuscle cells is nonmuscle myosin II (NM II). This study was aimed to examine the functional role of NM II in resting and activated microglia. Using immunohistochemistry, we demonstrate strong expression of NM II isoform B (NM IIB) in microglia during cuprizone-induced demyelination as well as in cultured microglia. Treatment with the NM II inhibitor blebbistatin prevented the morphological shaping of microglial cells, led to functional deficits during chemokine-directed migration and phagocytosis, induced NM IIB redistribution, and affected actin microfilament patterning. In addition, inhibition of NM II led to an attenuated release of nitric oxide (NO), while TNFα secretion was not altered. In conclusion, we propose a pivotal role of NM II in cytoskeleton organization during microglial activation. This is of great importance to understand the mechanisms of microglial action in inflammatory CNS diseases.

    Topics: Animals; Animals, Newborn; Brain; Cell Movement; Cells, Cultured; Chemokine CCL2; Cuprizone; Demyelinating Diseases; Disease Models, Animal; Dose-Response Relationship, Drug; Heterocyclic Compounds, 4 or More Rings; Male; Mice, Inbred C57BL; Microglia; Monoamine Oxidase Inhibitors; Myosin Heavy Chains; Nonmuscle Myosin Type IIB; Phagocytosis; Rats; Rats, Sprague-Dawley

2014