bleomycin-a6 and Pulmonary-Fibrosis

IH764-3 is a potent component isolated from Salvia miltiorrhiza. We have studied the effect of IH764-3 on experimental pulmonary fibrosis in rats and mice treated with a single intratracheal instillation of bleomycin-A6. Three groups of animals were assigned as BLM+saline, BLM+IH764-3 and normal control. The results indicated that in the treated group, lung coefficient, surfactant, hydroxyproline content and FGF activity were significantly lower than those in the control group (P < 0.05, 0.05, 0.001 and 0.05, respectively). Electron microscopic examination showed that pulmonary ultrastructure was markedly better in the treated group: type II alveolar epithelial cells, infiltrating inflammatory cells, proliferating collagen-forming cells, collagen and elastic fibers were obviously fewer in the treated group. These results demonstrate that IH764-3 has prophylactic and therapeutic effects on pulmonary fibrosis.

Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Bleomycin; Drugs, Chinese Herbal; Fibroblast Growth Factors; Hydroxyproline; Lung; Mice; Pulmonary Fibrosis; Rats; Rats, Wistar

IH764-3, an effective component isolated from Salvae milliorrhize (a component of traditional Chinese medicine) was used against pulmonary fibrosis in this study. The results indicated that in the treated group, lung coefficient, surfactant, hydroxyproline content and FGF activity were significantly lower than those in the model group. Electron microscopic observation confirmed that the pulmonary ultrastructure was improved remarkably in the treated group: the proliferation of collagen-forming cells and infiltration of inflammatory cells, collagen and elastic fibers were obviously less. All the results demonstrated that IH764-3 has prophylactic and therapeutic effects on the development of pneumonitis and pulmonary fibrosis.

Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Bleomycin; Bronchoalveolar Lavage Fluid; Drug Combinations; Drugs, Chinese Herbal; Fibroblast Growth Factors; Hydroxyproline; Male; Mice; Phenanthrolines; Plant Extracts; Pulmonary Fibrosis; Rats; Rats, Wistar; Salvia miltiorrhiza; Surface-Active Agents