biotin has been researched along with Sensitivity and Specificity in 589 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 6 (1.02) | 18.7374 |
| 1990's | 233 (39.56) | 18.2507 |
| 2000's | 233 (39.56) | 29.6817 |
| 2010's | 102 (17.32) | 24.3611 |
| 2020's | 15 (2.55) | 2.80 |
| Authors | Studies |
|---|---|
| Jang, M; Kim, S; Song, J | 1 |
| Ahmed, M; Tabatabaei, MS | 1 |
| Chen, H; Cui, P; Ding, Y; Hua, X; Wang, M; Wu, X; Zong, L | 1 |
| Cao, H; Jiang, G; Mao, K; Ran, F; Xu, P; Yang, Z; Zhang, H; Zhang, X; Zhao, Y; Zhou, H | 1 |
| Hwang, SH; Kim, SK; Ko, DH; Oh, HB; Oh, YH; Sung, H | 1 |
| Matson, RS | 1 |
| Bhat, AI; Greeshma, M; Jeevalatha, A | 1 |
| Agarwal, S; Bier, FF; Hamidizadeh, M | 1 |
| Faschinger, F; Gruber, HJ; Hytönen, VP; Knör, G; Oberbichler, E; Schlögl, E; Stangl, J; Wiesauer, M | 1 |
| Ayadi, N; Benhelli-Mokrani, H; Charlier, C; Fleury, F; Lafont, F; Nabiev, I; Sokolov, P; Sukhanova, A | 1 |
| Chen, F; Du, P; Ke, C; Lei, B; Liang, L; Liu, Z; Peng, X; Sun, C; Wang, L; Wen, S; Wu, J; Xu, X; Yang, J; Zeng, G; Zhang, H; Zhang, W; Zhang, X; Zheng, H; Zou, L | 1 |
| Chaix, C; Farre, C; Lejal, N; Manzano, M; Robin, P; Vidic, J; Viezzi, S; Wright, A | 1 |
| Bezinge, L; Dobson, PJ; Dold, G; Gliddon, HD; Gray, ER; Heaney, J; Huang, D; McKendry, RA; Miller, BS; Morton, JJL; Nastouli, E | 1 |
| Ding, S; Feng, K; Huang, G; Jin, L; Li, T; Liang, X; Wu, B; Yang, T; Yue, X; Zhang, J | 1 |
| Aktas, GB; Masip, L; Ribera, A; Skouridou, V | 1 |
| Klem, T; Niu, H; Pan, L; Qiu, Y; Yang, J | 1 |
| Coucke, W; Cox, E; De Craeye, S; Dierick, K; Dorny, P; Geerts, M; Gisbert Algaba, I; Jennes, M; Opsteegh, M | 1 |
| Afroz, T; Ao, W; Berlinghoff, E; Denys, G; Jenison, R; Jensen, J; Klonoski, J; Lindsey, W; Munns, D | 1 |
| Chen, W; Li, B; Lu, J; Xue, F; Yan, C; Yao, L; Zhang, J | 1 |
| Livingston, KE; Mahoney, JP; Manglik, A; Sunahara, RK; Traynor, JR | 1 |
| Prins, MWJ; van IJzendoorn, LJ; Visser, EWA; Yan, J | 1 |
| Dong, C; Gao, H; Geng, Y; Hu, J; Huang, R; Jiang, C; Jing, J; Sun, X; Wang, Y; Wang, Z; Wei, X | 1 |
| Macdonald, PJ; Ruan, Q; Tetin, SY | 1 |
| Alekseev, LP; Drobyazina, PE; Maerle, AV; Pivovarov, VD; Ryazantsev, DY; Simonova, MA; Trofimov, DY; Voronina, DV; Zavriev, SK | 1 |
| Chen, Q; Liu, X; Sun, Z; Tang, Z; Wang, X | 1 |
| Jiang, H; Liang, D; Mari, GM; Peng, T; Sun, S; Zeng, Y; Zheng, P | 1 |
| Huang, Y; Liu, D; Liu, F; Song, Y; Yang, C; Zhou, SF; Zhu, Z | 1 |
| Ali, Z; Deng, Y; He, N; Li, K; Li, X; Li, Z; Lu, G; Ma, C; Ma, N; Mou, X; Tang, Y; Yang, H; Zhang, L; Zou, J | 1 |
| Collyer, SD; Davis, F; Higson, SP; Holford, TR; Holmes, JL | 1 |
| Chen, G; Hu, Z; Liu, J; Ye, Y; Yu, L; Zou, Y | 1 |
| Kinoshita, E; Kinoshita-Kikuta, E; Koike, T | 1 |
| Bai, Y; Duan, Y; Feng, F; He, X; Qin, J; Tian, M; Wang, C; Wang, H; Zhao, L | 1 |
| Ederth, T; Nicholls, IA; Petrone, L; Suriyanarayanan, S | 1 |
| Han, W; Shi, M; Spivack, SD | 1 |
| Kumar, JS; Lakshmana Rao, PV; Parida, M | 1 |
| Fan, H; Li, Y; Liu, Y; Yang, P; Yao, F | 1 |
| Gassler, N; Peuscher, A; Rasche, S; Schillberg, S; Schneider, U; Spiegel, H; Thom, P | 1 |
| Burley, GA; Dawson, MD; Guilhabert, B; Haughey, AM; Kanibolotsky, AL; Laurand, N; Skabara, PJ | 1 |
| Bernard, A; Caldejon, S; Dee, N; Harris, JA; Henry, AM; Hirokawa, KE; Hohmann, JG; Joines, KM; Jones, AR; Mortrud, M; Nyhus, J; Oh, SW; Ouellette, B; Parry, S; Wang, Q; Zeng, H | 1 |
| Chaube, SK; Kariya, KP; Kumar, D; Prasad, PK; Shrivastav, TG | 1 |
| Bu, D; Yang, GX; Zhuang, HS | 1 |
| Berg, K; Bostad, M; Høgset, A; Kausberg, M; Olsen, CE; Selbo, PK; Weyergang, A | 1 |
| Chen, J; Deng, B; Zhang, H | 1 |
| Cheng, B; Liu, Y; Saremi, B; Wei, MY; Yuan, B | 1 |
| Carr, M; Fields, C; Hall, W; Lee, GU; Liauchuk, V; Muzard, J; Ran, YF | 1 |
| Gong, X; Hu, Y; Ou, W; Wan, Y; Zhu, M | 1 |
| Ahn, YH; Bhagwat, AS; Shalhout, S; Wei, S | 1 |
| Li, Z; Liu, C; Sun, S; Tang, Y; Zhang, Y | 1 |
| Awsiuk, K; Budkowski, A; Fornal, K; Kakabakos, SE; Koukouvinos, G; Mavrogiannopoulou, E; Petrou, PS; Siafaka-Kapadai, A; Yannoukakos, D | 1 |
| Baruah, KK; Mondal, M; Prakash, BS | 1 |
| He, X; Patfield, SA | 1 |
| Gao, H; Ke, XD; Liu, Q; Shen, S; Song, LJ; Wang, J; Xu, YL; Yin, XR; Yu, CX | 1 |
| Jang, G; Kim, D; Kim, J; Lee, TS; Noh, J | 1 |
| Ooijevaar-de Heer, P; Rispens, T | 1 |
| Kim, C; Kim, H; Kwon, K; Lee, J; Ree, M | 1 |
| Li, B; Lv, W; Ma, W; Situ, B; Vadgama, P; Wang, W; Yin, X; Zheng, L | 1 |
| Chu, PK; Dong, Y; Jiang, L; Li, Y; Wei, Q; Zhang, Y | 1 |
| Gopinath, SC; Lakshmipriya, T; Tang, TH | 1 |
| Kiatpathomchai, W; Nawattanapaiboon, K; Prombun, P; Santanirand, P; Srikhirin, T; Sutapun, B; Vongsakulyanon, A | 1 |
| Alapan, Y; De Luca, A; ElKabbash, M; Gurkan, UA; Hinczewski, M; Ilker, E; Sreekanth, KV; Strangi, G | 1 |
| Bai, W; Chen, A; Huang, Y; Yan, J; Yan, M; Zhao, Y; Zhu, C | 1 |
| Arakawa, H; Haraya, S; Karasawa, K; Kato, N; Ozawa, K; Sano, Y | 1 |
| Astilean, S; Campu, A; Craciun, AM; Focsan, M; Leordean, C; Maniu, D; Potara, M | 1 |
| Guo, Q; Han, JJ; Lai, WH; Liu, DF; Shan, S; Wu, SS; Xiong, YH | 1 |
| Passos, JF; Salmonowicz, H | 1 |
| Cheol, CS; Rahman, MA; Shiddiky, MJ; Shim, YB | 1 |
| Gamella, M; Pingarrón, JM; Reviejo, AJ; Serra, B | 1 |
| Ambroz, KL; Olive, DM; Schutz-Geschwender, A; Zhang, Y | 1 |
| Jiang, L; Jiang, T; Lu, Z; Tang, Z; Yu, Z; Zhang, C | 1 |
| Chen, GN; Li, ZJ; Lin, JM; Lin, Z; Ren, SQ; Wang, X; Ying, XT | 1 |
| Guo, L; Li, T; Wang, Z | 1 |
| Lowery, TJ; Meldrum, T; Pines, A; Schröder, L; Smith, M; Wemmer, DE | 1 |
| Cho, JH; Chung, BH; Chung, SJ; Kim, M; Park, HG; Park, HK | 1 |
| Therriault, D | 1 |
| Choi, YK; Gu, B; Huang, XJ; Im, H | 1 |
| Awazu, K; Fujimaki, M; Gopinath, SC; Gupta, KC; Komatsubara, T; Kumar, PK; Ohki, Y; Sugimoto, K; Tominaga, J | 1 |
| Grossmann, TN; Röglin, L; Seitz, O | 1 |
| Christopoulos, TK; Ioannou, PC; Konstantou, JK | 1 |
| Guo, LH; Zhang, Q; Zhu, S | 1 |
| Aguilera, A; Argento Martell, L; Boyer, R; Cox, C; Crockett, L; Foehr, E; Prince, WS; Van Tuyl, A; White, JT | 1 |
| Hayman, RB; Li, Z; Walt, DR | 1 |
| Diaz, MR; Dunbar, SA; Jacobson, JW | 1 |
| Collot, M; Danzé, PM; Drucbert, AS; Fievez, A; Mallet, JM; Poulain, D; Savaux, C; Sendid, B; Standaert-Vitse, A; Tabouret, M | 1 |
| Korf, J; Posthuma-Trumpie, GA; van Amerongen, A | 1 |
| Guymon, CA | 1 |
| Chaube, SK; Kariya, KP; Nara, S; Rangari, K; Shrivastav, TG; Singh, H; Tripathi, V | 2 |
| Ataka, K; Heberle, J; Jiang, X; Zuber, A | 1 |
| Karnik, R; Karp, JM; Sarkar, D; Spelke, D; Teo, GS; Vemula, PK; Wee, le Y | 1 |
| Juul-Madsen, HR; Jørgensen, AL; Stagsted, J | 1 |
| Alieva, EV; Konopsky, VN | 3 |
| Im, H; Lesuffleur, A; Lindquist, NC; Oh, SH | 1 |
| Meyerhoff, ME; Shen, D | 1 |
| Chen, H; Li, X; Zhan, Z; Zhang, S | 1 |
| Choi, SJ; Mun, S | 1 |
| Austin, CP; Inglese, J; Regan, L; Southall, N; Yi, F; Zheng, W; Zhu, P | 1 |
| Xing, D; Zhou, H; Zhou, X; Zhu, D | 1 |
| Gronewold, TM | 1 |
| Iida, H; Kajiura, M; Nakanishi, T; Osaka, T; Takada, H | 1 |
| Chiu, JK; Ho, JA; Hong, JC; Hwang, KC; Hwu, JR; Lin, CC | 1 |
| Forrester, MT; Foster, MW; Moseley, MA; Nogueira, L; Stamler, JS; Thompson, JW | 1 |
| Hänninen, PE; Härmä, H; Määttänen, A; Näreoja, T; Peltonen, J | 1 |
| Duan, MH; Wang, NY; Zhang, D; Zhang, YC; Zhao, W | 1 |
| Hampe, CS; Landin-Olsson, M; Lernmark, A; Oak, S; Radtke, J; Törn, C | 1 |
| Anderson, D; Azizi, A; Bozic, J; Diaz-Mitoma, F; Golshani, A; Sirskyj, D; Weltzin, R | 1 |
| Doria, R; Erba, P; Lazzeri, E; Mariani, G; Perri, M; Tascini, C | 1 |
| Ferrara, KW; Hu, X; Kruse, DE; Stephens, DN; Sutcliffe, P; Zheng, H | 1 |
| Baker, JR; Divin, C; Guo, Y; Huang, B; Norris, TB; Thomas, TP; Ye, JY | 1 |
| Guo, Z; Su, P; Yang, Y; Zhang, W | 1 |
| Fan, C; He, Y; Hu, M; Lu, HT; Song, S; Wang, LH; Weng, LX; Yan, J | 1 |
| He, Y; Lu, S; Ruan, C; Zhang, Y; Zhao, Y; Zhu, M | 1 |
| Chai, Y; Cui, H; Tian, D; Wang, W | 1 |
| Coleman, D; Fox, M; Good, J; Jiang, J; Lewin-Koh, SC; Lowe, J; Peng, K; Qiu, ZJ; Quarmby, V; Rahman, A; Song, A; Yang, J; Ying, Y | 1 |
| Chang, HC; Chen, ML; Chiu, JK; Ho, JA; Hsu, WL; Li, CC; Liao, WC | 1 |
| Alegret, S; Campoy, S; Fabiano, S; García, MI; Lermo, A; Liébana, S; Pividori, MI; Soutullo, A; Zumárraga, MJ | 1 |
| Chang, JS; Eom, SC; Huh, C; Lee, S; Shin, JH; Sung, GY | 1 |
| Chase-Baldwin, K; Padilla, S; Taitt, CR; Vora, GJ; Wasieloski, LP; Wojciechowski, J | 1 |
| Fischer, SK; Peng, K; Quarmby, V; Siradze, K | 1 |
| He, N; Li, S; Liu, H | 1 |
| Burgoyne, JR; Eaton, P; Mayr, M; Rudyk, O | 1 |
| Chen, KH; Foo, YL; Hobley, J; Su, X | 1 |
| Christopoulos, TK; Elenis, DS; Ioannou, PC | 1 |
| Kim, YM; Kwon, HS; Lee, JH; Yang, S; Yoo, SK; Yoon, SY | 1 |
| Liao, JC; Mach, KE; Mohan, R; Riahi, R; Wong, PK | 1 |
| He, D; Hua, W; Huang, L; Liu, P; Liu, T; Na, N; Ouyang, J | 1 |
| Chen, K; Han, H; Jiang, X | 1 |
| He, Z; Liu, A; Wu, L; Zhou, J | 1 |
| D'Agata, R; Spoto, G; Zanoli, LM | 1 |
| Boysen, G; Horvath, TD; Matthews, NI; Mock, DM; Stratton, SL | 1 |
| Hasebe, T; Tabata, H; Yamada, Y | 1 |
| Kubota, LT; Linares, EM; Michaelis, J; Thalhammer, S | 1 |
| Ahn, DJ; Choi, H; Choi, IS; Lee, GS | 1 |
| Armani, AM; Hunt, HK; Soteropulos, C | 1 |
| Cao, L; Chang, K; Chen, M; Chen, W; Deng, S; Ding, Y; Jia, S; Li, F; Lu, W; Shi, J; Xu, Q; Zhang, K | 1 |
| Boleininger, A; Hami, S; Lake, T; Vallance, C | 1 |
| Gupta, RK; Malhotra, BD; Singh, R; Sood, S; Srivastava, AK; Sumana, G; Verma, R | 1 |
| Ai, S; Meng, X; Yin, H; Zhang, H; Zhou, Y | 1 |
| Das, D; Lowary, TL; Sarkar, S; Spencer, JS; Suresh, MR; Tang, XL | 1 |
| Chen, W; Cheng, S; Jiang, X; Shi, F; Wang, L; Zhu, C | 1 |
| Antón-Badiola, I; González-Carreró, J; Ortiz-Rey, JA; Pérez-Pedrosa, A; Peteiro-Cancelo, Á | 1 |
| Xing, D; Zhou, X; Zhu, D | 1 |
| Fukada, Y; Kinoshita, E; Kinoshita-Kikuta, E; Koike, T; Ozeki, T; Sugiyama, Y | 1 |
| Inouye, S; Sato, J | 1 |
| Ang, WH; Chan, Y; Ho, HK; Pastorin, G; Tian, Q; Wong, W; Xu, Y | 1 |
| Guo, J; Ju, J; Kalachikov, SM; Kumar, S; Lu, J; Naini, AB; Qiu, C; Russo, JJ; Schon, EA; Shi, S | 1 |
| Ferru, E; Pantaleo, A; Turrini, F | 1 |
| Deng, Y; He, N; Li, S; Li, X; Liu, H; Mou, X; Xu, L | 1 |
| Li, HQ; Sun, H; Wang, XQ; Yang, J; Zeng, ZW; Zhao, Q | 1 |
| Caprioli, RM; Mernaugh, RL; Parl, FF; Spraggins, JM; Thiery, G; Yan, H; Yang, J | 1 |
| Bütikofer, L; Faust, H; Lemaillet, G | 1 |
| Cho, NJ; Höök, F; Rabe, M; Tabaei, SR; Zhdanov, VP | 1 |
| Chen, C; Wu, J | 1 |
| Ametis-Cabello, J; Ballesta-Claver, J; Capitán-Vallvey, LF; Megía-Fernández, A; Morales-Sanfrutos, J; Santoyo-González, F; Valencia-Mirón, MC | 1 |
| Iwamaru, Y; Mohri, S; Okada, H; Yokoyama, T | 1 |
| Cioffi, N; Gristina, R; Magliulo, M; Mallardi, A; Palazzo, G; Ridi, F; Sabbatini, L; Torsi, L | 1 |
| Coward, JK; Srinivasan, A | 1 |
| Cunningham, B; Gerstenmeier, J; Li, P; Lin, B; Pepper, J; Pien, H; Qiu, J | 1 |
| Haes, AJ; Van Duyne, RP | 1 |
| Goh, B; Goh, MC; Loo, RW; McAloney, RA | 1 |
| De Boer, PA; Deprez, RH; Fijnvandraat, AC; Moorman, AF | 1 |
| Mankin, AS; Polacek, N; Shinabarger, D; Swaney, S | 1 |
| Ju, J | 1 |
| Guesdon, JL; Mouratou, B; Pauillac, S; Rouyre, S | 1 |
| Ikebukuro, K; Kohiki, Y; Sode, K | 1 |
| Abbott, D; Liu, H; MacColl, R; Mannella, CA; Menikh, A; Mickan, SP; Munch, J; Zhang, XC | 1 |
| Cosnier, S; Marks, RS; Novoa, A; Thomassey, D | 1 |
| Beatty, BG; Cooper, K; Evans, MF; Mount, SL | 1 |
| Duff, SE; Kumar, S; Li, C; O'Dwyer, ST; Renehan, A | 1 |
| Li, B; Li, G; Yang, H; Yang, S; Zhuang, Y | 1 |
| Kim, BG; Kim, YK; Lee, CS; Lee, SH; Park, SS | 1 |
| Cabral, JM; Ferreira, HA; Freitas, PP; Graham, DL | 1 |
| Goto, J; Hirama, M; Hishinuma, T; Itoh, K; Kato, Y; Mizugaki, M; Oguri, H; Suzuki, T; Tomioka, Y; Tsuruta, LR | 1 |
| Banks, RE; Craven, RA; Harnden, PA; Selby, PJ | 1 |
| Amoroso, M; Ananieva-Jordanova, R; Arnold, C; Betterle, C; Brooking, H; Furmaniak, J; Powell, M; Smith, BR; Zanchetta, R | 1 |
| Gutstein, HB | 1 |
| Bailey, C; Ferrer, M; Gardell, SJ; Inglese, J; Kolodin, G; Mao, SS; Peltier, RR; Strulovici, B; Zuck, P | 1 |
| Boudou, P; Fiet, J; Galons, H; Giton, F; Ibrahim, F; Julien, R; Villette, JM | 1 |
| Berney, C; Danuser, G | 1 |
| Mori, Y; Muneta, Y; Splichal, I; Takahashi, E | 1 |
| Carpenter, JE; Ellegala, DB; Kaul, S; Klibanov, AL; Leong-Poi, H; Lindner, JR; Shaffrey, ME; Sklenar, J | 1 |
| Burns, DJ; Chen, CM; David, CA; Lim, B; Middleton, TR; Pithawalla, R; Tripathi, RL; Warrior, U; Xuei, X | 1 |
| Hampe, CS; Lernmark, A; Luo, D; Rogers, CN | 1 |
| Anderson, CJ; Axworthy, DB; Fritzberg, AR; Lewis, MR; Mallet, RW; Theodore, LJ; Wang, M; Welch, MJ | 1 |
| Jyotsna, N; Paradkar, SN; Sivaprasad, N; Vrinda, C | 1 |
| LICHSTEIN, HC | 1 |
| Borchers, CH; Glish, GL; Lee, DC; Parker, CE; Pope, RM; Raska, CS; Sunnarborg, SW | 1 |
| Adar, F; Campbell, WB; Hawi, SR; McCoy, MJ; Nakamoto, K; Nithipatikom, K | 1 |
| Brand, S; Cross, GH; Freeman, NJ; Peel, LL; Popplewell, JF; Reeves, AA; Swann, MJ | 1 |
| Chinnaiyan, A; Copeland, S; Knight, PR; Laxman, B; Remick, DG; Siddiqui, J; Sreekumar, A | 1 |
| Geddes, CD; Lakowicz, JR; Moore, WE; Pugh, VJ; Szmacinski, H | 1 |
| Flowers, L; Horowitz, IR; Huang, R; Huang, RP; Lin, Y; Parthasarathy, S; Ramachandran, S; Shi, Q | 1 |
| Kakabakos, SE; Misiakos, K; Petrou, PS; Ruf, HH | 1 |
| Bret, G; Kellenberger, E; Müller, P; Paul, N; Rognan, D | 1 |
| Varnum, SM; Woodbury, RL; Zangar, RC | 1 |
| Huang, RP | 1 |
| Kim, O | 1 |
| Bouwman, K; Costa, J; Dillon, D; Haab, BB; Lizardi, P; Marrero, JA; Schotanus, M; Verweij, C; Zhou, H | 1 |
| Cheng, VC; Hui, WT; Seto, WH; Wang, LN; Yam, WC; Yuen, KY | 1 |
| Hytönen, VP; Kulomaa, MS; Laitinen, OH; Nordlund, HR; Porkka, E; Uotila, ST | 1 |
| Gilliam, LK; Hampe, CS; Lernmark, A; Luo, D; Rogers, CN; Steed, JT | 1 |
| Caca, K; Huster, D; Kress, S; Mössner, J; Weizenegger, M | 1 |
| Kokuryu, Y; Matsunaga, T; Takeda, H; Tanaka, T | 1 |
| Bodei, L; Bottoni, A; Consoli, V; Cristofani, R; Erba, PA; Lazzeri, E; Manca, M; Mariani, G; Palestro, CJ; Pauwels, EK; Trippi, D; Volterrani, D | 1 |
| Fidaa, I; Fiet, J; Galons, H; Giton, F; Raynaud, JP; Valleix, A | 1 |
| Boozer, C; Chen, S; Homola, J; Jiang, S; Ladd, J; Yu, Q | 1 |
| Eggli, V; Glockshuber, R; Kuennemann, E; Textor, M; Vörös, J; Zammaretti, P; Zhen, G | 1 |
| Lowery, TJ; Pines, A; Rubin, SM; Ruiz, EJ; Schultz, PG; Spence, MM; Wemmer, DE; Winssinger, N | 1 |
| Costa-García, A; Díaz-González, M; González-García, MB; Hernández-Santos, D | 1 |
| Falconnet, D; Höök, F; Pfeiffer, I; Städler, B; Vörös, J | 1 |
| Bornhop, DJ; Markov, DA; Swinney, K | 1 |
| Bischoff, S; Fromme, M; Gromnica-Ihle, E; Gross, J; Haselbeck, A; Hoesel, W; Kanne, B; Moller, R; Müller, A; Müller, G; Wessner, A | 1 |
| Härmä, H; Huhtinen, P; Lövgren, T; Soukka, T | 1 |
| Brennan, JP; Eaton, P; Neubert, H; Saurin, AT | 1 |
| Brückl, H; Kamp, PB; Panhorst, M; Reiss, G | 1 |
| Devi, NA; Doss, VA; Parvathi, S; Raju, BA | 1 |
| Greenberg, MM; Sato, K | 1 |
| Adam, V; Bailey, M; Howard, JA; Jelen, F; Kizek, R; Klejdus, B; Kramer, KJ; Masarik, M; Mikelova, R; Potesil, D; Trnkova, L | 1 |
| Dhali, A; Mondal, M; Prakash, B; Prakash, BS; Rajkhowa, C | 1 |
| Cataldi, TR; Nardiello, D | 1 |
| Graves, SW; Kim, H; Nolan, JP; Woods, TA | 1 |
| Hasui, K; Murata, F | 1 |
| Mirzaei, H; Regnier, F | 1 |
| Grunwald, C; Kuhlmann, J; Mirsky, VM; Niemax, K; Wolfbeis, OS; Zybin, A | 1 |
| Aguilar, JC; Coiras, MT; López-Campos, G; López-Huertas, MR; Pérez-Breña, P | 1 |
| Ho, JA; Huang, MR | 1 |
| Mukhopadhyay, R | 1 |
| Amoroso, MA; Ananieva-Jordanova, R; Bennett, S; Brooking, H; Burne, P; Chen, S; Collins, A; Furmaniak, J; Maclean, C; Mitchell, S; Powell, M; Smith, BR; Willis, J | 1 |
| Aguilar, ZP; Boyaci, IH; Halsall, HB; Heineman, WR; Hossain, M; Seliskar, CJ | 1 |
| Brosstad, F; Michelsen, AE; Stokke, O; Wergeland, R | 1 |
| Hahn, MA; Krauss, TD; Tabb, JS | 1 |
| Bergman, R; Fontaine, NH; Mazumder, P; Mozdy, EJ; Quesada, MA; Yuen, PK | 1 |
| Jung, YK; Kim, JM; Park, HG | 1 |
| Amemiya, Y; Matsunaga, T; Tanaka, T; Yoza, B | 1 |
| Gray, B; Podniesinski, E; Stewart, CC; Woodring, ML | 1 |
| Besse, PA; Ehrmann, K; Gersbach, M; Massin, C; Pascoal, P; Popovic, RS; Stamou, D; Vincent, F; Vogel, H | 1 |
| Beck, M; Charbonnière, LJ; Hildebrandt, N; Löhmannsröben, HG; Ziessel, RF | 1 |
| Fischer-Durand, N; Jaouen, G; Pradier, CM; Salmain, M; Yam, CM | 1 |
| Cao, C; Chae, H; Kim, BW; Kim, JP; Sim, SJ; Yang, SS; Yoon, HC | 1 |
| Borghs, G; Bosmans, E; Brys, L; Conrath, K; Frederix, F; Huang, L; Jans, K; Maes, G; Muyldermans, S; Reekmans, G; Saerens, D | 1 |
| Denu, JM; Garske, AL | 1 |
| Arakaki, A; Iida, H; Matsunaga, T; Nakanishi, T; Niwa, D; Osaka, T | 1 |
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| Diamandis, EP; Scorilas, A | 1 |
| Dembrow, D; Deryugina, E; Leng, J; Marchenko, G; Ratnikov, B; Strongin, A | 1 |
| Massey, SC; Mills, SL | 1 |
| Cricca, M; Gallinella, G; Gentilomi, G; Musiani, M; Venturoli, S; Zerbini, M | 1 |
| Cheung, AL; Dandachi, N; Dietze, O; Graf, AH; Hacker, GW; Hauser-Kornberger, C; Tubbs, RR | 1 |
| Clavijo, A; Fernando, L; Ridd, D; Riva, J; Zhou, EM | 1 |
| Fenwick, B; Inzana, TJ | 1 |
| Belosludtsev, I; Belosludtsev, Y; Hogan, M; Iverson, B; Lemeshko, S; Powdrill, T; Wiese, R | 1 |
| Lövgren, T; Pettersson, K; Qin, QP | 1 |
| Beck, Z; Bégány, A; Gergely, L; Hunyadi, J; Juhász, A; Kónya, J; Medgyessy, I; Remenyik, E; Veress, G | 1 |
| Han, SQ; He, YF; Wu, FB | 2 |
| Boudi, A; Boudou, P; Fiet, J; Galons, H; Giton, F; Soliman, H; Villette, JM | 1 |
| Mock, DM; Nyalala, JO; Raguseo, RM | 1 |
| Cui, Y; Lieber, CM; Park, H; Wei, Q | 1 |
| Klemic, JF; Reed, MA; Stern, E | 1 |
| Edwards, JR; Itagaki, Y; Ju, J | 1 |
| Buranda, T; Lopez, GP; Pastuszyn, A; Simons, P; Sklar, LA | 1 |
| Azizi, F; Hedayati, M; Yazdanparast, R | 1 |
| Dong, le V; Eng, KH; Gopalakrishnakone, P; Selvanayagam, ZE | 1 |
| Chilkoti, A; Nath, N | 1 |
| Glezos, N; Kakabakos, S; Misiakos, K; Petrou, P; Terzoudi, G | 1 |
| Howard, M; Kaplan, D | 1 |
| Bergmann, P; Franzson, L; Hermsen, D; Hoffmann, JP; Isaksson, A; Kaufman, JM; Leary, E; Müller, C; Nakatsuka, K; Nishizawa, Y; Reinauer, H; Riesen, W; Roth, HJ; Steinmüller, T; Troch, T | 1 |
| Delgado, RF; Fernández, RJ; Luque, de CM | 1 |
| He, X; Li, J; Tan, W; Tang, Z; Wang, K; Xiao, D; Yang, X | 1 |
| Ishimura, K; Kumagai, I; Nakamura, M; Tsumoto, K | 1 |
| Busse, S; Menges, B; Mittler, S; Scheumann, V | 1 |
| Mock, DM; Mock, NI | 1 |
| Goodman, MN; Jacobberger, JW; Sladek, TL; Srivastava, P | 1 |
| Imagawa, DT; Kusakabe, H; Lee, MH; Mizoguchi, J; Nakai, M; Otani, M; Sano, K; Takasaki, T; Tanno, M; Urabe, T | 1 |
| Chin, CC; Shao, MC | 1 |
| Festin, SM; Floyd, DM; Hedberg, A; Hunt, JT; Liu, EC; Natarajan, S | 1 |
| Ojeh, CK; Saif, LJ; Simkins, RA; Tsunemitsu, H | 1 |
| Lee, LH | 1 |
| Grogan, TM; Hanson, CA; Ross, CW; Schlegelmilch, JA; Schnitzer, B; Weiss, LM | 1 |
| Gratama, JW; Medenblik, AM; Raap, AK; Tanke, HJ; van der Ploeg, M; van Gijlswijk, RP; Vlieger, AM | 1 |
| Hassam, S; Pospischil, A; Siegl, G; Stoerckle, N; Tratschin, JD; Waldvogel, AS; Weilenmann, R | 1 |
| Bauman, JG; de Jong, MO; Rozemuller, H; Visser, JW | 1 |
| Hashida, S; Ishikawa, E | 1 |
| Raap, AK; Tanke, HJ; van Gijlswijk, RP | 1 |
| Asbahr, H; Davies, JM; Erber, WN; Herrmann, RP; Meyer, B | 1 |
| Bodourian, SH; Henkel, CF; Pesce, MA; Sheehan, M | 1 |
| Friedman, K; Gudibande, SR; Kenten, JH; Link, J; Massey, RJ | 1 |
| Ellis, J; Halliday, G | 1 |
| Babín, M; Dominguez, J; Laviada, MD; Sánchez-Vizcaíno, JM | 1 |
| Lüke, FJ; Schlegel, W | 1 |
| Benveniste, J; Collé, A; Leibowitch, J; Leung-Tack, J; Manuel, Y; Prévot, D; Tavera, C; Thomas, Y | 1 |
| Arnold, N; Kessler, C; Seibl, R; Wienberg, J | 1 |
| Kano, T; Okamoto, N; Suzuki, K; Watanabe, S | 1 |
| Bereziat, G; Beuret, T; Charlotte, F; Chatelet, F; Chypre, C; Luboinski, J; Marchand, J; Olivier, JL; Sadoul, G | 1 |
| Dissanayake, S; Min, X; Piessens, WF | 1 |
| Arnholdt, H; Diebold, J; Lai, MD; Löhrs, U | 1 |
| Johnson, TL; Kini, SR; Zarbo, RJ | 1 |
| Hall, TC; Huntley, CC; Marsh, LE; Rao, AL | 1 |
| Babín, M; Domínguez, J; Hedrick, RP; Sánchez, C | 1 |
| Christopoulos, TK; Diamandis, EP; Wilson, G | 1 |
| Ackermann, M; Kihm, U; Mettraux, C; Steiger, Y | 1 |
| Brandt, CD; Herzog, KD; Josephs, SH; Parrott, RH; Rakusan, TA; Saxena, ES; Sever, JL; Sison, AV | 1 |
| Ishikawa, N; Nakamura, A; Suzuki, H | 1 |
| Allen, JR; Blacklow, NR; Carcamo, C; Gary, HE; Herrmann, JE; Humphrey, CD; Kim, KH; Koch, M; Moe, CL; Monroe, SS | 1 |
| Anderson, JF; Magnarelli, LA | 1 |
| Anderson, BE; Kim, JG; LaBarbera, AR; Rebar, RW | 1 |
| Helgeland, L; Myrset, AH | 1 |
| Baenziger, JU; Mengeling, BJ; Smith, DF; Smith, PL; Stults, NL | 1 |
| Allan, GM; Allen, IV; Cosby, SL; McQuaid, S; Smith, J; Taylor, MJ; Todd, D | 1 |
| Crampton, JM; Hill, SM; Urwin, R | 1 |
| Bains, SK; Baker, TJ; Fattah, DI; Hissey, PH; McNamee, LA | 1 |
| Alvarez-Bolado, G; Catsicas, S; Lebeau, MC; Wahli, W | 1 |
| al-Riyami, A; Campbell, AM; Haynes, LG | 1 |
| Hansen, K; Lebech, AM; Pii, K | 1 |
| Matsunaga, H; Miyoshi, K; Nakagami, S; Yamane, A | 1 |
| Kessler, C; Schmitz, GG; Seibl, R; Walter, T | 1 |
| Furukawa, S; Furukawa, Y; Hayashi, K; Murase, K; Takeuchi, R | 1 |
| Czerwenka, KF; Knogler, W; Kubista, E; Manavi, M; Schatten, C; Schön, HJ | 1 |
| Arita, M; Hirayama, A; Inoue, M; Kodama, K; Takagaki, Y; Tsuji, A | 1 |
| Al-Ahdal, MN; Khan, GY; Qadri, SG; Qadri, SM | 1 |
| Corbitt, G; Kimpton, CP; Morris, DJ | 1 |
| Cheng, HM; Prasad, U; Sam, CK; Thoe, SY | 1 |
| Habili, N; McInnes, JL; Symons, RH | 1 |
| Hlozánek, I; König, J; Korcová, J; Korec, E | 1 |
9 review(s) available for biotin and Sensitivity and Specificity
| Article | Year |
|---|---|
Functionalized gold nanoparticles for ultrasensitive DNA detection.
Topics: Biosensing Techniques; Biotin; Colorimetry; DNA; Electrochemistry; Gold; Metal Nanoparticles; Quartz Crystal Microbalance Techniques; Sensitivity and Specificity; Silver; Surface Plasmon Resonance | 2012 |
Synthesis of magnetic nanoparticles and their application to bioassays.
Topics: Biotin; Ferric Compounds; Magnetics; Micelles; Nanoparticles; Organosilicon Compounds; Sensitivity and Specificity; Streptavidin; Surface Properties | 2006 |
In situ hybridization using cRNA probes: isotopic and nonisotopic detection methods.
Topics: Animals; Autoradiography; Biotin; Digoxigenin; Humans; In Situ Hybridization; Nucleic Acid Amplification Techniques; Nucleic Acid Hybridization; RNA, Complementary; RNA, Messenger; Sensitivity and Specificity; Tyramine | 2006 |
Activity-based protein profiling: new developments and directions in functional proteomics.
Topics: Animals; Biotin; Enzymes; Fluorescent Dyes; Molecular Structure; Molecular Weight; Protein Array Analysis; Proteomics; Sensitivity and Specificity | 2008 |
Detection of proteins on blots using the avidin-biotin system.
Topics: Animals; Avidin; Biotin; Blotting, Western; Humans; Immunoenzyme Techniques; Mice; Proteins; Rabbits; Sensitivity and Specificity | 1994 |
Determinations of biotin in biological fluids.
Topics: Animals; Avidin; Biological Assay; Biotin; Chromatography, High Pressure Liquid; Humans; Sensitivity and Specificity | 1997 |
Competitive enzymatic assay of biotin.
Topics: Animals; Binding, Competitive; Biological Assay; Biotin; Humans; Sensitivity and Specificity | 1997 |
Amplification methods to increase the sensitivity of in situ hybridization: play card(s).
Topics: Biotin; Catalysis; Histocytochemistry; Horseradish Peroxidase; In Situ Hybridization; Nucleic Acids; Sensitivity and Specificity; Tyramine | 1999 |
Applications of enzymatic amplification staining in immunophenotyping hematopoietic cells.
Topics: Biotin; Flow Cytometry; Fluorescent Antibody Technique; Hematopoietic Stem Cells; Horseradish Peroxidase; Humans; Immunophenotyping; Lymphocyte Subsets; Neoplasms; Sensitivity and Specificity; Staining and Labeling; Tyramine | 2002 |
7 trial(s) available for biotin and Sensitivity and Specificity
| Article | Year |
|---|---|
Development of glutamic acid decarboxylase 65 (GAD65) autoantibody assay using biotin-GAD65 fusion protein.
Topics: Autoantigens; Biomarkers; Biotin; Blood Chemical Analysis; Diabetes Mellitus, Type 1; Enzyme-Linked Immunosorbent Assay; Glutamate Decarboxylase; Humans; Recombinant Fusion Proteins; Reproducibility of Results; Sensitivity and Specificity | 2004 |
Clinical feasibility of two-step streptavidin/111In-biotin scintigraphy in patients with suspected vertebral osteomyelitis.
Topics: Adult; Aged; Aged, 80 and over; Biotin; Feasibility Studies; Female; Humans; Male; Middle Aged; Osteomyelitis; Pentetic Acid; Radionuclide Imaging; Reproducibility of Results; Sensitivity and Specificity; Spine; Spondylitis | 2004 |
Lymphocyte propionyl-CoA carboxylase and its activation by biotin are sensitive indicators of marginal biotin deficiency in humans.
Topics: Adult; Analysis of Variance; Biomarkers; Biotin; Cross-Over Studies; Deficiency Diseases; Female; Humans; Lymphocytes; Male; Methylmalonyl-CoA Decarboxylase; Nutrition Assessment; Nutritional Status; Sensitivity and Specificity; Valerates | 2006 |
Avidin and 111In-labelled biotin scan: a new radioisotopic method for localising vascular graft infection.
Topics: Aged; Aorta; Avidin; Biotin; Blood Vessel Prosthesis; Female; Femoral Artery; Follow-Up Studies; Humans; Iliac Artery; Indium Radioisotopes; Male; Middle Aged; Polyethylene Terephthalates; Polytetrafluoroethylene; Popliteal Artery; Predictive Value of Tests; Prospective Studies; Prosthesis Design; Prosthesis-Related Infections; Sensitivity and Specificity; Tomography, Emission-Computed, Single-Photon; Treatment Outcome | 1995 |
Improved detection of minimal residual leukemia through modifications of polymerase chain reaction analyses based on clonospecific T cell receptor junctions.
Topics: Alleles; Base Sequence; Biotin; Child; Clone Cells; DNA Primers; Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; Humans; Molecular Sequence Data; Neoplasm Proteins; Neoplasm, Residual; Polymerase Chain Reaction; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Prospective Studies; Receptors, Antigen, T-Cell, gamma-delta; Sensitivity and Specificity; T-Lymphocytes | 1995 |
Clinical evaluation of a modified ELISA, using photobiotinylated DNA, for the detection of anti-DNA antibodies.
Topics: Antibodies, Antinuclear; Autoimmune Diseases; Biotin; DNA; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Fluorescent Antibody Technique; Humans; Reproducibility of Results; Sensitivity and Specificity | 1994 |
Immunohistochemical staining of dystrophin on formalin-fixed paraffin-embedded sections in Duchenne/Becker muscular dystrophy and manifesting carriers of Duchenne muscular dystrophy.
Topics: 3,3'-Diaminobenzidine; Adolescent; Adult; Antibodies, Monoclonal; Biotin; Child; Child, Preschool; Coloring Agents; Dystrophin; Female; Heterozygote; Humans; Hydrogen Peroxide; Immunohistochemistry; Male; Muscle, Skeletal; Muscular Dystrophy, Duchenne; Peroxidase; Sarcolemma; Sensitivity and Specificity; Streptavidin; Tyramine | 2000 |
573 other study(ies) available for biotin and Sensitivity and Specificity
| Article | Year |
|---|---|
Highly sensitive and rapid detection of porcine circovirus 2 by avidin-biotin complex based lateral flow assay coupled to isothermal amplification.
Topics: Animals; Avidin; Biotin; Circovirus; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques; Sensitivity and Specificity; Swine | 2021 |
Enzyme-Linked Immunosorbent Assay (ELISA).
Topics: Biotin; Enzyme-Linked Immunosorbent Assay; Sensitivity and Specificity | 2022 |
Biotin-labelled peptidomimetic for competitive time-resolved fluoroimmunoassay of benzothiostrobin.
Topics: Acrylates; Benzothiazoles; Biotin; Fluoroimmunoassay; Ligands; Peptides; Peptidomimetics; Sensitivity and Specificity; Streptavidin | 2022 |
Paper Device Combining CRISPR/Cas12a and Reverse-Transcription Loop-Mediated Isothermal Amplification for SARS-CoV-2 Detection in Wastewater.
Topics: Biotin; CRISPR-Cas Systems; Fluoresceins; Nucleic Acid Amplification Techniques; Pandemics; RNA, Viral; SARS-CoV-2; Sensitivity and Specificity; Wastewater | 2022 |
Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus.
Topics: Biotin; Digoxigenin; DNA; Hepacivirus; Hepatitis C; Humans; Nanoparticles; Polystyrenes; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity; Streptavidin | 2022 |
ELISA Essentials: Surfaces, Antibodies, Enzymes, and Substrates.
Topics: Antibodies; Biotin; Enzyme-Linked Immunosorbent Assay; Sensitivity and Specificity; Streptavidin | 2023 |
Rapid onsite detection of piper yellow mottle virus infecting black pepper by recombinase polymerase amplification-lateral flow assay (RPA-LFA).
Topics: Biotin; Nucleic Acid Amplification Techniques; Piper nigrum; Plant Viruses; Polymerase Chain Reaction; Recombinases; Sensitivity and Specificity | 2023 |
Detection of Reverse Transcriptase LAMP-Amplified Nucleic Acid from Oropharyngeal Viral Swab Samples Using Biotinylated DNA Probes through a Lateral Flow Assay.
Topics: Biotin; COVID-19; DNA; DNA Probes; Fluorescein-5-isothiocyanate; Humans; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques; Nucleic Acids; RNA-Directed DNA Polymerase; RNA, Viral; SARS-CoV-2; Sensitivity and Specificity | 2023 |
Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein.
Topics: Anilino Naphthalenesulfonates; Avidin; Binding Sites; Binding, Competitive; Biological Assay; Biotin; Biotinylation; DNA; Exonucleases; Fluoresceins; Sensitivity and Specificity; Streptavidin | 2020 |
Comparative Advantages and Limitations of Quantum Dots in Protein Array Applications.
Topics: Antibodies; Biotin; Fluorescent Dyes; HeLa Cells; High-Throughput Screening Assays; Humans; Immunoassay; Microarray Analysis; Protein Array Analysis; Quantum Dots; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2020 |
High-coverage SARS-CoV-2 genome sequences acquired by target capture sequencing.
Topics: Biotin; COVID-19; DNA Probes; DNA, Single-Stranded; Genome, Viral; Genotype; Humans; Mutation; Nasopharynx; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; SARS-CoV-2; Sensitivity and Specificity; Whole Genome Sequencing | 2020 |
Specific and sensitive detection of Influenza A virus using a biotin-coated nanoparticle enhanced immunomagnetic assay.
Topics: Antibodies, Monoclonal; Biotin; Immunomagnetic Separation; Influenza A virus; Nanoparticles; Sensitivity and Specificity | 2022 |
Spin-enhanced nanodiamond biosensing for ultrasensitive diagnostics.
Topics: Avidin; Biosensing Techniques; Biotin; Early Diagnosis; Fluorescence; Gold; HIV Infections; HIV-1; Humans; Limit of Detection; Metal Nanoparticles; Microfluidics; Microwaves; Nanodiamonds; Nucleic Acid Amplification Techniques; Paper; Plasma; Quantum Theory; RNA, Viral; Sensitivity and Specificity; Single Molecule Imaging; Temperature | 2020 |
Rapid detection of Salmonella in milk with a nuclear magnetic resonance biosensor based on a streptavidin-biotin system and a polyamidoamine-dendrimer-targeted gadolinium probe.
Topics: Animals; Antigen-Antibody Reactions; Biosensing Techniques; Biotin; Dendrimers; Female; Filtration; Foodborne Diseases; Gadolinium; Magnetic Resonance Spectroscopy; Milk; Polyamines; Salmonella; Sensitivity and Specificity; Spectroscopy, Fourier Transform Infrared; Streptavidin; Time Factors | 2021 |
DNA immobilization and detection using DNA binding proteins.
Topics: Adsorption; Binding Sites; Biosensing Techniques; Biotechnology; Biotin; Collodion; Detergents; DNA; DNA-Binding Proteins; DNA, Single-Stranded; Immobilized Nucleic Acids; Limit of Detection; Membranes, Artificial; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2021 |
A biotin-drug extraction and acid dissociation (BEAD) procedure to eliminate matrix and drug interference in a protein complex anti-drug antibody (ADA) isotype specific assay.
Topics: Acids; Antibodies; Antibodies, Neutralizing; Biotin; Humans; Immunoglobulin G; Insulin-Like Growth Factor Binding Protein 3; Insulin-Like Growth Factor I; Recombinant Proteins; Sensitivity and Specificity | 2017 |
A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat.
Topics: Animals; Biological Assay; Biotin; DNA, Protozoan; Lod Score; Magnetic Fields; Meat; Mice; Microspheres; Nucleic Acid Hybridization; Real-Time Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Swine; Toxoplasma | 2017 |
Rapid Detection and Differentiation of Clinically Relevant Candida Species Simultaneously from Blood Culture by Use of a Novel Signal Amplification Approach.
Topics: Biotin; Candida; Candidemia; DNA, Fungal; Humans; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques; Reproducibility of Results; RNA, Ribosomal, 28S; Sensitivity and Specificity; Time Factors | 2018 |
Aptamer-mediated colorimetric method for rapid and sensitive detection of chloramphenicol in food.
Topics: Animals; Aptamers, Nucleotide; Biotin; Chloramphenicol; Colorimetry; Fish Products; Food Analysis; Food Contamination; Honey; Horseradish Peroxidase; Limit of Detection; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2018 |
Measuring ligand efficacy at the mu-opioid receptor using a conformational biosensor.
Topics: Allosteric Regulation; Allosteric Site; Biosensing Techniques; Biotin; Humans; Interferometry; Ligands; Loperamide; Methadone; Protein Binding; Receptors, Opioid, mu; Sensitivity and Specificity; Single-Domain Antibodies; Small Molecule Libraries; Solutions; Streptavidin; Thiophenes; Urea | 2018 |
Continuous biomarker monitoring by particle mobility sensing with single molecule resolution.
Topics: Animals; Biomarkers; Biosensing Techniques; Biotin; Cattle; DNA; DNA Probes; DNA, Single-Stranded; Humans; Kinetics; Magnetite Nanoparticles; MicroRNAs; Microscopy, Video; Monitoring, Physiologic; Sensitivity and Specificity; Single Molecule Imaging; Streptavidin; Thrombin | 2018 |
Development of duplex PCR-ELISA for simultaneous detection of Salmonella spp. and Escherichia coli O157: H7 in food.
Topics: Animals; Bacterial Proteins; Biotin; Brassica; Carbohydrate Epimerases; Cattle; Chickens; Colony Count, Microbial; DNA Primers; DNA, Bacterial; Enzyme-Linked Immunosorbent Assay; Escherichia coli O157; Food Contamination; Food Microbiology; Foodborne Diseases; Fruit and Vegetable Juices; Humans; Limit of Detection; Milk; Molecular Probe Techniques; Nucleic Acid Hybridization; Polymerase Chain Reaction; Red Meat; Salmonella; Seafood; Sensitivity and Specificity; Streptomycin; Time Factors; Transaminases | 2018 |
Direct single-molecule counting for immunoassay applications.
Topics: Biotin; Carbocyanines; HIV Core Protein p24; Immunoassay; Microscopy, Fluorescence; Sensitivity and Specificity; Single Molecule Imaging; Streptavidin | 2019 |
Development of the covalent antibody-DNA conjugates technology for detection of IgE and IgM antibodies by immuno-PCR.
Topics: Animals; Antibodies, Monoclonal; Antigens; Biotin; Biotinylation; DNA; Humans; Immunoglobulin E; Immunoglobulin M; Mice; Oligonucleotides; Polymerase Chain Reaction; Sensitivity and Specificity; Streptavidin | 2019 |
Development of a biotin-streptavidin-amplified nanobody-based ELISA for ochratoxin A in cereal.
Topics: Biotin; Edible Grain; Enzyme-Linked Immunosorbent Assay; Limit of Detection; Ochratoxins; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Tandem Mass Spectrometry | 2019 |
Immunochromatographic fluorometric determination of clenbuterol with enhanced sensitivity.
Topics: Animals; Antibodies; Biotin; Chromatography, Affinity; Clenbuterol; Collodion; Fluorescent Dyes; Fluorometry; Gold; Immunoassay; Limit of Detection; Membranes, Artificial; Metal Nanoparticles; Particle Size; Sensitivity and Specificity; Streptavidin; Surface Properties; Swine | 2019 |
Catalase-linked immunosorbent pressure assay for portable quantitative analysis.
Topics: Biotin; C-Reactive Protein; Catalase; Catalysis; Enzyme-Linked Immunosorbent Assay; Humans; Limit of Detection; Metal Nanoparticles; Oxygen; Platinum; Pressure; Sensitivity and Specificity | 2019 |
Highly sensitive and rapid detection of Pseudomonas aeruginosa based on magnetic enrichment and magnetic separation.
Topics: Bacteriological Techniques; Biotin; DNA Gyrase; DNA, Bacterial; Humans; Luminescent Measurements; Magnetics; Nanoparticles; Polymerase Chain Reaction; Pseudomonas aeruginosa; Sensitivity and Specificity; Staining and Labeling | 2013 |
Label-free impedimetric immunosensors for psoriasin--increased reproducibility and sensitivity using an automated dispensing system.
Topics: Adsorption; Aniline Compounds; Antibodies, Immobilized; Avidin; Biosensing Techniques; Biotin; Carbon; Electric Impedance; Electrodes; Humans; Immunoassay; S100 Calcium Binding Protein A7; S100 Proteins; Sensitivity and Specificity; Static Electricity | 2013 |
A hypersensitive biotin-avidin-TRFIA for quantitative detection of ANA-Ig(GAM) and its clinical application.
Topics: Animals; Antibodies, Antinuclear; Arthritis, Rheumatoid; Avidin; Biotin; Enzyme-Linked Immunosorbent Assay; Fluoroimmunoassay; Humans; Liver Cirrhosis, Biliary; Lupus Erythematosus, Systemic; Mice; Mixed Connective Tissue Disease; Observer Variation; Scleroderma, Systemic; Sensitivity and Specificity; Sjogren's Syndrome | 2013 |
Sandwich assay for phosphorylation of protein multiplexes by using antibodies and Phos-tag.
Topics: Antibodies; Biotin; Luminescence; Phosphoproteins; Protein Array Analysis; Proteins; Pyridines; Sensitivity and Specificity | 2013 |
Aptamer/thrombin/aptamer-AuNPs sandwich enhanced surface plasmon resonance sensor for the detection of subnanomolar thrombin.
Topics: Aptamers, Nucleotide; Biosensing Techniques; Biotin; Gold; Humans; Limit of Detection; Metal Nanoparticles; Sensitivity and Specificity; Surface Plasmon Resonance; Thrombin | 2013 |
Biotinyl moiety-selective polymer films with highly ordered macropores.
Topics: Biotin; Biotinylation; Molecular Imprinting; Polymers; Porosity; Quartz Crystal Microbalance Techniques; Sensitivity and Specificity | 2013 |
A quantitative method to identify microRNAs targeting a messenger RNA using a 3'UTR RNA affinity technique.
Topics: 3' Untranslated Regions; Animals; Biological Assay; Biotin; Cloning, Molecular; Epithelial Cells; Gene Expression Regulation; Genes, Reporter; Homeodomain Proteins; Humans; Immobilized Nucleic Acids; Liver; Luciferases; Mice; MicroRNAs; Microspheres; Real-Time Polymerase Chain Reaction; Respiratory Mucosa; Sensitivity and Specificity; Smad4 Protein; Streptavidin; Transcription Factors; Zinc Finger E-box-Binding Homeobox 1 | 2013 |
Development & evaluation of biotinylated DNA probe for clinical diagnosis of chikungunya infection in patients' acute phase serum & CSF samples.
Topics: Alphavirus Infections; Animals; Biotin; Cell Line; Chikungunya Fever; Chlorocebus aethiops; DNA Probes; Electrophoresis, Agar Gel; Enzyme-Linked Immunosorbent Assay; Humans; Nucleic Acid Hybridization; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity; Vero Cells | 2013 |
Detection and quantification of lysine acetyl-alteration using antibody microarray.
Topics: Acetylation; Animals; Antibodies; Biomarkers, Tumor; Biotin; Carcinoma, Hepatocellular; Cattle; Humans; Liver Neoplasms; Lysine; Protein Array Analysis; Protein Processing, Post-Translational; Reproducibility of Results; Sensitivity and Specificity; Serum Albumin, Bovine; Streptavidin; Vascular Endothelial Growth Factor A | 2013 |
An immunohistochemical assay on human tissue using a human primary antibody.
Topics: Animals; Antibodies, Monoclonal; Biotin; Biotinylation; Carbocyanines; Carcinoembryonic Antigen; Carcinoma; CHO Cells; Colonic Neoplasms; Cricetulus; Fluorescent Antibody Technique, Indirect; HEK293 Cells; Humans; Immunoglobulin G; Mice; Sensitivity and Specificity; Staining and Labeling; Streptavidin | 2014 |
An oligofluorene truxene based distributed feedback laser for biosensing applications.
Topics: Animals; Avidin; Biosensing Techniques; Biotin; Biotinylation; Cattle; Equipment Design; Fluorenes; Lasers; Semiconductors; Sensitivity and Specificity; Serum Albumin, Bovine | 2014 |
Systematic comparison of adeno-associated virus and biotinylated dextran amine reveals equivalent sensitivity between tracers and novel projection targets in the mouse brain.
Topics: Animals; Biotin; Brain; Cell Count; Dependovirus; Dextrans; Fluorescent Dyes; Immunohistochemistry; Mice, Inbred C57BL; Microscopy, Confocal; Microscopy, Fluorescence; Neural Pathways; Neuroanatomical Tract-Tracing Techniques; Neuronal Tract-Tracers; Neurons; Photomicrography; Sensitivity and Specificity | 2014 |
Development of enzyme-linked immunosorbent assay for estimation of urinary albumin.
Topics: Adolescent; Adult; Albuminuria; Animals; Biotin; Case-Control Studies; Cross Reactions; Diabetes Mellitus, Type 2; Enzyme-Linked Immunosorbent Assay; Female; Haplorhini; Horseradish Peroxidase; Horses; Humans; Hydrogen Peroxide; Immune Sera; Immunoconjugates; Kidney; Male; Middle Aged; Sensitivity and Specificity; Serum Albumin; Streptavidin | 2014 |
A sensitive enzyme-linked immunosorbent assay amplified by biotin-streptavidin system for detecting non-steroidal anti-inflammatory drug ketoprofen.
Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Aquaculture; Biotin; Carps; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Food Analysis; Food Contamination; Inhibitory Concentration 50; Ketoprofen; Limit of Detection; Perciformes; Rabbits; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2014 |
Light-triggered, efficient cytosolic release of IM7-saporin targeting the putative cancer stem cell marker CD44 by photochemical internalization.
Topics: Antibodies, Monoclonal; Biotin; Cell Line, Tumor; Cytosol; Drug Carriers; Endosomes; Flow Cytometry; Humans; Hyaluronan Receptors; Immunotoxins; Light; Lysosomes; Neoplastic Stem Cells; Photochemotherapy; Photosensitizing Agents; Reactive Oxygen Species; Ribosome Inactivating Proteins, Type 1; Saporins; Sensitivity and Specificity; Streptavidin; Time Factors | 2014 |
Assembly of multiple DNA components through target binding toward homogeneous, isothermally amplified, and specific detection of proteins.
Topics: Biotin; Chemistry Techniques, Analytical; DNA; DNA, Bacterial; Endonucleases; Fluorescence; Limit of Detection; Molecular Probe Techniques; Prostate-Specific Antigen; Proteins; Sensitivity and Specificity; Streptavidin | 2014 |
Re-evaluation of biotin-streptavidin conjugation in Förster resonance energy transfer applications.
Topics: Artifacts; Biotin; DNA; Fluorescence Resonance Energy Transfer; Immunoassay; Protein Interaction Mapping; Quantum Dots; Reproducibility of Results; Sensitivity and Specificity; Staining and Labeling; Streptavidin | 2014 |
Rapid, highly sensitive detection of herpes simplex virus-1 using multiple antigenic peptide-coated superparamagnetic beads.
Topics: Amino Acid Sequence; Antibodies, Viral; Antigens, Viral; Biotin; Flow Cytometry; Herpesvirus 1, Human; Magnetics; Protein Binding; Sensitivity and Specificity; Streptavidin; Viral Proteins | 2014 |
Streptavidin-biotin-based directional double Nanobody sandwich ELISA for clinical rapid and sensitive detection of influenza H5N1.
Topics: Amino Acid Sequence; Biotin; Enzyme-Linked Immunosorbent Assay; Humans; Influenza A Virus, H5N1 Subtype; Influenza, Human; Molecular Sequence Data; Sensitivity and Specificity; Sequence Homology, Amino Acid; Single-Domain Antibodies; Streptavidin | 2014 |
A versatile new tool to quantify abasic sites in DNA and inhibit base excision repair.
Topics: Alkynes; Biotin; Cell Death; DNA; DNA Damage; DNA Mutational Analysis; DNA Repair; DNA-(Apurinic or Apyrimidinic Site) Lyase; HeLa Cells; Humans; Hydroxylamines; Methyl Methanesulfonate; Sensitivity and Specificity | 2015 |
Phosphorylation-induced hybridization chain reaction on beads: an ultrasensitive flow cytometric assay for the detection of T4 polynucleotide kinase activity.
Topics: Bacteriophage T4; Biological Assay; Biotin; Flow Cytometry; Fluorescence; Microspheres; Nucleic Acid Hybridization; Phosphorylation; Polynucleotide 5'-Hydroxyl-Kinase; Sensitivity and Specificity; Streptavidin; Viral Proteins | 2015 |
Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates.
Topics: Animals; Base Pairing; Biotin; Biotinylation; BRCA1 Protein; Cattle; DNA Mutational Analysis; Humans; Mutation; Oligonucleotide Array Sequence Analysis; Oligonucleotides; Polymerase Chain Reaction; Protein Binding; Sensitivity and Specificity; Serum Albumin, Bovine; Streptavidin; Time Factors | 2015 |
Development and Application of a Sensitive, Second Antibody Format Enzymeimmunoassay (EIA) for Estimation of Plasma FSH in Mithun (Bos frontalis).
Topics: Animals; Animals, Wild; Antibodies; Biotin; Cattle; Estrous Cycle; Female; Follicle Stimulating Hormone; Goats; Gonadotropin-Releasing Hormone; Immune Sera; Immunoconjugates; Immunoenzyme Techniques; Peroxidase; Rabbits; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2016 |
Immuno-PCR Assay for Sensitive Detection of Proteins in Real Time.
Topics: Antibodies, Monoclonal; Antigens; Biotin; Buffers; DNA; DNA Restriction Enzymes; Immunoassay; Immunoconjugates; Polymerase Chain Reaction; Ricin; Ricinus; Sensitivity and Specificity; Streptavidin; Time Factors | 2015 |
[Establishment and diagnostic performance of biotin-avidin complex enzyme linked immunosorbent assay of detecting specific IgG4 of clonorchiasis].
Topics: Antibodies, Helminth; Avidin; Biotin; Clonorchiasis; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin G; Sensitivity and Specificity | 2015 |
Functionalized, Fluorescent, Conjugated Polymer Nanospheres for Protein Targeting via Förster Resonance Energy Transfer.
Topics: Biotin; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Materials Testing; Molecular Probe Techniques; Nanoconjugates; Nanospheres; Particle Size; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Surface Properties | 2015 |
Quantification of the degree of biotinylation of proteins using proteinase K digestion and competition ELISA.
Topics: Antibodies; Biotin; Biotinylation; Endopeptidase K; Enzyme-Linked Immunosorbent Assay; Proteins; Sensitivity and Specificity; Streptavidin | 2016 |
Self-Assembly-Assisted Biomolecule-Enriched Surface and High Selectivity Performance of Simple Solution-Coatable Biomimicking Brush Copolymers.
Topics: Adsorption; Avidin; Biomimetic Materials; Biosensing Techniques; Biotin; Phosphorylcholine; Polyethylene Glycols; Polymers; Protein Binding; Sensitivity and Specificity | 2016 |
Electrochemical determination of microRNAs based on isothermal strand-displacement polymerase reaction coupled with multienzyme functionalized magnetic micro-carriers.
Topics: Biosensing Techniques; Biotin; Breast; Breast Neoplasms; Electrochemical Techniques; Female; Humans; Limit of Detection; Magnets; MicroRNAs; Nanoparticles; Nucleic Acid Amplification Techniques; Sensitivity and Specificity; Streptavidin | 2016 |
A sandwich-type electrochemical immunosensor based on the biotin- streptavidin-biotin structure for detection of human immunoglobulin G.
Topics: Biosensing Techniques; Biotin; Humans; Immunoglobulin G; Indicators and Reagents; Magnetics; Nanoparticles; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2016 |
Biotin-Streptavidin Competition Mediates Sensitive Detection of Biomolecules in Enzyme Linked Immunosorbent Assay.
Topics: Antibodies; Antigens, Bacterial; Biotin; Enzyme-Linked Immunosorbent Assay; Humans; Sensitivity and Specificity; Streptavidin | 2016 |
Hemoculture and Direct Sputum Detection of mecA-Mediated Methicillin-Resistant Staphylococcus aureus by Loop-Mediated Isothermal Amplification in Combination With a Lateral-Flow Dipstick.
Topics: Bacteriological Techniques; Base Sequence; Biotin; DNA Primers; Electrophoresis, Agar Gel; Genes, Bacterial; Humans; Limit of Detection; Methicillin-Resistant Staphylococcus aureus; Nucleic Acid Amplification Techniques; Polymerase Chain Reaction; Reproducibility of Results; Rheology; Sensitivity and Specificity; Sputum | 2016 |
Extreme sensitivity biosensing platform based on hyperbolic metamaterials.
Topics: Biotin; Sensitivity and Specificity; Streptavidin; Surface Plasmon Resonance | 2016 |
A sandwich dipstick assay for ATP detection based on split aptamer fragments.
Topics: Adenosine Triphosphate; Aptamers, Nucleotide; Biotin; Gold; Limit of Detection; Metal Nanoparticles; SELEX Aptamer Technique; Sensitivity and Specificity | 2016 |
Development of a highly specific enzyme immunoassay for oxytocin and its use in plasma samples.
Topics: Animals; Antibodies; Autistic Disorder; Avidin; Biotin; Biotinylation; Cattle; Colorimetry; Horseradish Peroxidase; Humans; Immunoconjugates; Immunoenzyme Techniques; Oxytocin; Polylysine; Rabbits; Reproducibility of Results; Sensitivity and Specificity | 2017 |
A simple and efficient design to improve the detection of biotin-streptavidin interaction with plasmonic nanobiosensors.
Topics: Antigen-Antibody Reactions; Biosensing Techniques; Biotin; Equipment Design; Equipment Failure Analysis; Gold; Immunoassay; Nanotechnology; Nanotubes; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Surface Plasmon Resonance | 2016 |
DNA-based hybridization chain reaction and biotin-streptavidin signal amplification for sensitive detection of Escherichia coli O157:H7 through ELISA.
Topics: Biotin; DNA, Bacterial; Enzyme-Linked Immunosorbent Assay; Equipment Design; Equipment Failure Analysis; Escherichia coli; Escherichia coli O157; Immunoassay; In Situ Hybridization, Fluorescence; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2016 |
Detecting senescence: a new method for an old pigment.
Topics: Aging; Antibodies; Azo Compounds; Biological Assay; Biomarkers; Biotin; Cell Cycle; Cellular Senescence; Humans; Lipofuscin; Naphthalenes; Sensitivity and Specificity; Staining and Labeling | 2017 |
Fabrication of disposable sensors for biomolecule detection using hydrazine electrocatalyst.
Topics: Avidin; Biotin; Biotinylation; Catalysis; Chemistry Techniques, Analytical; Dendrimers; Disposable Equipment; DNA; Electrochemistry; Electrodes; Gold; Humans; Hydrazines; Hydrogen Peroxide; Immunoassay; Metal Nanoparticles; Oxidation-Reduction; Proteins; Sensitivity and Specificity; Surface Properties; Water | 2008 |
Lectin-modified piezoelectric biosensors for bacteria recognition and quantification.
Topics: Adsorption; Bacteria; Biosensing Techniques; Biotin; Concanavalin A; Crystallization; Electrochemistry; Electrodes; Escherichia coli; Gold; Lectins; Mycobacterium phlei; Quartz; Sensitivity and Specificity; Staphylococcus aureus | 2008 |
Blocking and detection chemistries affect antibody performance on reverse phase protein arrays.
Topics: Animals; Antibodies; Antibodies, Blocking; Antibody Specificity; Biotin; Blotting, Western; Fluorescence; Fluorescent Antibody Technique, Indirect; Indicators and Reagents; Indoles; Luminescence; Mice; Protein Array Analysis; Rats; Sensitivity and Specificity; Spectrophotometry, Infrared; Streptavidin; Tyramine | 2008 |
Protein arrays based on biotin-streptavidin system for the simultaneous detection of TORCH infections.
Topics: Biotin; Cytomegalovirus Infections; Enzyme-Linked Immunosorbent Assay; Herpes Simplex; Humans; Protein Array Analysis; Reagent Kits, Diagnostic; Rubella; Sensitivity and Specificity; Streptavidin; Toxoplasmosis | 2008 |
Development of a sensitive, rapid, biotin-streptavidin based chemiluminescent enzyme immunoassay for human thyroid stimulating hormone.
Topics: Animals; Antibodies, Monoclonal; Antibody Specificity; Biotin; Biotinylation; Calibration; Cattle; Female; Horseradish Peroxidase; Humans; Hydrogen Peroxide; Immunoenzyme Techniques; Kinetics; Luminescence; Luminol; Mice; Pregnancy; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Thyrotropin; Time Factors | 2008 |
Gold nanoparticle-based surface enhanced Raman scattering spectroscopic assay for the detection of protein-protein interactions.
Topics: Antibodies, Monoclonal; Avidin; Binding Sites; Biotin; Gold; Immunoglobulin G; Metal Nanoparticles; Nanotechnology; Particle Size; Scattering, Radiation; Sensitivity and Specificity; Spectrum Analysis, Raman; Staphylococcal Protein A | 2008 |
Temperature response of 129Xe depolarization transfer and its application for ultrasensitive NMR detection.
Topics: Avidin; Biotin; Macromolecular Substances; Nuclear Magnetic Resonance, Biomolecular; Polycyclic Compounds; Proteins; Sensitivity and Specificity; Temperature; Triazoles; Xenon Isotopes | 2008 |
Mixed self-assembly of polydiacetylenes for highly specific and sensitive strip biosensors.
Topics: Albuminuria; Biosensing Techniques; Biotin; Diabetic Nephropathies; Hydrogen-Ion Concentration; Polyacetylene Polymer; Polymers; Polyynes; Reagent Strips; Sensitivity and Specificity | 2008 |
Biosensors: filling the gap.
Topics: Biosensing Techniques; Biotin; Equipment Design; Equipment Failure Analysis; Nanotechnology; Sensitivity and Specificity; Streptavidin; Transistors, Electronic | 2007 |
A dielectric-modulated field-effect transistor for biosensing.
Topics: Biosensing Techniques; Biotin; Equipment Design; Equipment Failure Analysis; Nanotechnology; Sensitivity and Specificity; Streptavidin; Transistors, Electronic | 2007 |
Influence of nanometric holes on the sensitivity of a waveguide-mode sensor: label-free nanosensor for the analysis of RNA aptamer-ligand interactions.
Topics: Aptamers, Nucleotide; Biosensing Techniques; Biotin; Ligands; Nanotechnology; Protein Binding; Sensitivity and Specificity; Staining and Labeling; Streptavidin | 2008 |
Target-catalyzed transfer reactions for the amplified detection of RNA.
Topics: Biotin; Catalysis; Enzyme-Linked Immunosorbent Assay; HIV-1; Molecular Probes; Peptide Nucleic Acids; Polymerase Chain Reaction; RNA; Sensitivity and Specificity | 2008 |
Dual-allele dipstick assay for genotyping single nucleotide polymorphisms by primer extension reaction.
Topics: Antibodies, Immobilized; Aryl Hydrocarbon Hydroxylases; Biotin; Cytochrome P-450 CYP2C19; Digoxigenin; DNA Mutational Analysis; DNA Primers; Genotype; Humans; Oligonucleotides; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Reagent Strips; Sensitivity and Specificity; Toll-Like Receptor 4 | 2009 |
Part-per-trillion level detection of estradiol by competitive fluorescence immunoassay using DNA/dye conjugate as antibody multiple labels.
Topics: Animals; Antibodies, Monoclonal; Benzothiazoles; Biotin; Cross Reactions; Diamines; DNA; Estradiol; Fluorescent Antibody Technique, Direct; Fluorescent Dyes; Fluoroimmunoassay; Goats; Luminescent Measurements; Mice; Organic Chemicals; Quinolines; Sensitivity and Specificity; Streptavidin | 2008 |
Comparison of neutralizing antibody assays for receptor binding and enzyme activity of the enzyme replacement therapeutic Naglazyme (galsulfase).
Topics: Antibodies; Antibody Formation; Biotin; Enzyme-Linked Immunosorbent Assay; Humans; In Vitro Techniques; N-Acetylgalactosamine-4-Sulfatase; Protein Binding; Receptor, IGF Type 2; Receptors, Cell Surface; Recombinant Proteins; Sensitivity and Specificity | 2008 |
Detection of single-molecule DNA hybridization using enzymatic amplification in an array of femtoliter-sized reaction vessels.
Topics: Biotin; DNA; Fiber Optic Technology; Microchemistry; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Oligonucleotide Probes; Sensitivity and Specificity | 2008 |
Multiplexed detection of fungal nucleic acid signatures.
Topics: Biological Assay; Biotin; Biotinylation; DNA Probes; DNA, Fungal; Flow Cytometry; Fluorescence; Fluorescent Dyes; Fungi; Humans; Microspheres; Mycoses; Nucleic Acid Amplification Techniques; Nucleic Acid Hybridization; Nucleic Acids; Oligonucleotide Probes; Opportunistic Infections; Polymerase Chain Reaction; Sensitivity and Specificity; Time Factors | 2008 |
Biotin sulfone as a new tool for synthetic oligosaccharide immobilization: application to multiple analysis profiling and surface plasmonic analysis of anti-Candida albicans antibody reactivity against alpha and beta (1-->2) oligomannosides.
Topics: Antibodies, Fungal; Avidin; Biosensing Techniques; Biotin; Candida albicans; Carbohydrate Conformation; Carbohydrate Sequence; Immunoassay; Molecular Sequence Data; Oligosaccharides; Sensitivity and Specificity; Sulfones; Surface Plasmon Resonance; Surface Properties | 2008 |
Development of a competitive lateral flow immunoassay for progesterone: influence of coating conjugates and buffer components.
Topics: Adsorption; Antibodies; Biotin; Buffers; Carbon; Collodion; Colloids; Enzyme-Linked Immunosorbent Assay; Haptens; Membranes, Artificial; Ovalbumin; Polyethylene Glycols; Progesterone; Reproducibility of Results; Sensitivity and Specificity; Serum Albumin, Bovine | 2008 |
High-sensitivity molecular recognition with light-induced polymerization.
Topics: Avidin; Biotechnology; Biotin; Molecular Diagnostic Techniques; Polymers; Sensitivity and Specificity; Ultraviolet Rays | 2008 |
Use of biotin-streptavidin system for developing a viable, sensitive and specific antigen heterologous assay for hapten.
Topics: Biotin; Esterification; Haptens; Molecular Structure; Radioimmunoassay; Sensitivity and Specificity; Streptavidin; Substrate Specificity | 2008 |
A novel enzyme-linked immunosorbent assay for cortisol using a long-chain biotinylated cortisol-3-CMO derivative.
Topics: Animals; Antibodies; Biotin; Enzyme-Linked Immunosorbent Assay; Humans; Hydrocortisone; Rabbits; Radioimmunoassay; Sensitivity and Specificity; Serum Albumin, Bovine; Streptavidin | 2008 |
In situ monitoring of the orientated assembly of strep-tagged membrane proteins on the gold surface by surface enhanced infrared absorption spectroscopy.
Topics: Adsorption; Amino Acid Transport System X-AG; Biological Transport; Biotin; Chemistry, Physical; Cysteamine; Escherichia coli; Glutamine; Gold; Models, Chemical; Protein Binding; Sensitivity and Specificity; Streptavidin; Surface Properties; Time Factors | 2008 |
Chemical engineering of mesenchymal stem cells to induce a cell rolling response.
Topics: Biotin; Cell Differentiation; Cell Survival; Fluorescence; Humans; Leukocyte Rolling; Mesenchymal Stem Cells; Oligosaccharides; P-Selectin; Sensitivity and Specificity; Sialyl Lewis X Antigen; Staining and Labeling; Streptavidin | 2008 |
A novel, simple and sensitive ligand affinity capture method for detecting molecular interactions by MALDI mass spectrometry.
Topics: Affinity Labels; Angiotensins; Avidin; Biotin; Biotinylation; Coumaric Acids; Lipopolysaccharides; Models, Molecular; Polymyxin B; Sensitivity and Specificity; Silanes; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2009 |
Optical biosensors based on photonic crystal surface waves.
Topics: Biosensing Techniques; Biotin; Equipment Design; Equipment Failure Analysis; Immunoassay; Optical Devices; Refractometry; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2009 |
Sub-micron resolution surface plasmon resonance imaging enabled by nanohole arrays with surrounding Bragg mirrors for enhanced sensitivity and isolation.
Topics: Biotin; Dimethylpolysiloxanes; Kinetics; Lab-On-A-Chip Devices; Microscopy, Atomic Force; Microscopy, Electron, Scanning; Nanotechnology; Sensitivity and Specificity; Streptavidin; Surface Plasmon Resonance | 2009 |
Pyrroloquinoline quinone-doped polymeric nanospheres as sensitive tracer for binding assays.
Topics: Acetonitriles; Animals; Avidin; Binding, Competitive; Biotin; C-Reactive Protein; Cattle; Glucose 1-Dehydrogenase; Humans; Immunoassay; Molecular Probe Techniques; Molecular Weight; Nanospheres; Polymethyl Methacrylate; PQQ Cofactor; Sensitivity and Specificity; Solvents | 2009 |
Enzyme enhanced quantitative determination of multiple DNA targets based on capillary electrophoresis.
Topics: Avidin; Biotin; DNA; Electrochemical Techniques; Electrophoresis, Capillary; Horseradish Peroxidase; Hydrogen-Ion Concentration; Linear Models; Reproducibility of Results; Sensitivity and Specificity | 2009 |
Optimization of the hybrid bilayer membrane method for immobilization of avidin on quartz crystal microbalance.
Topics: Adsorption; Avidin; Biosensing Techniques; Biotin; Computer-Aided Design; Equipment Design; Equipment Failure Analysis; Immunoassay; Lipid Bilayers; Membranes, Artificial; Micro-Electrical-Mechanical Systems; Protein Binding; Reproducibility of Results; Sensitivity and Specificity | 2009 |
An AlphaScreen-based high-throughput screen to identify inhibitors of Hsp90-cochaperone interaction.
Topics: Biotin; Biotinylation; Cell Line, Tumor; Drug Screening Assays, Antitumor; HSP90 Heat-Shock Proteins; Humans; Models, Biological; Molecular Chaperones; Protein Binding; Reproducibility of Results; Sensitivity and Specificity; Time Factors | 2009 |
Rapid and sensitive detection of point mutation by DNA ligase-based electrochemiluminescence assay.
Topics: 2,2'-Dipyridyl; Biosensing Techniques; Biotin; Cell Line; Coordination Complexes; DNA Ligases; Electrochemical Techniques; Genes, p53; Humans; Luminescent Measurements; Methods; Molecular Probes; Organometallic Compounds; Point Mutation; Sensitivity and Specificity; Streptavidin | 2009 |
Aptamers and biosensors.
Topics: Alkanes; Aptamers, Nucleotide; Biosensing Techniques; Biotin; Dextrans; Humans; Hydrogel, Polyethylene Glycol Dimethacrylate; Protein Binding; Sensitivity and Specificity; Streptavidin; Sulfhydryl Compounds; Thrombin | 2009 |
Biosensing by optical waveguide spectroscopy based on localized surface plasmon resonance of gold nanoparticles used as a probe or as a label.
Topics: Biosensing Techniques; Biotin; Gold; Metal Nanoparticles; Protein Binding; Sensitivity and Specificity; Streptavidin; Surface Plasmon Resonance | 2009 |
Gold-nanostructured immunosensor for the electrochemical sensing of biotin based on liposomal competitive assay.
Topics: Antibodies; Biosensing Techniques; Biotin; Electrochemistry; Electrodes; Ferrocyanides; Gold; Immunoassay; Liposomes; Nanostructures; Polyamines; Sensitivity and Specificity | 2009 |
Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture.
Topics: Biotin; Cell Line; Cysteine; Glyceraldehyde-3-Phosphate Dehydrogenases; Humans; Mass Spectrometry; Proteome; Proteomics; S-Nitrosothiols; Sensitivity and Specificity; Sulfhydryl Compounds | 2009 |
Impact of surface defects and denaturation of capture surface proteins on nonspecific binding in immunoassays using antibody-coated polystyrene nanoparticle labels.
Topics: Animals; Antibodies, Monoclonal; Binding Sites, Antibody; Biotin; Detergents; Equipment Design; Humans; Hydrogen-Ion Concentration; Immunoassay; Microscopy, Atomic Force; Microscopy, Fluorescence; Nanoparticles; Organometallic Compounds; Polystyrenes; Protein Denaturation; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Surface Properties; Temperature; Thyrotropin | 2009 |
[Clinical application of avidin-biotin ELISA to detect serum hepatoma-specific gamma-glutamyltransferase in patients with primary hepatic cancer].
Topics: Adult; alpha-Fetoproteins; Avidin; Biotin; Carcinoma, Hepatocellular; Enzyme-Linked Immunosorbent Assay; Female; gamma-Glutamyltransferase; Humans; Liver Neoplasms; Male; Middle Aged; Sensitivity and Specificity; Young Adult | 2009 |
Comparison of three assays for the detection of GAD65Ab-specific anti-idiotypic antibodies.
Topics: Absorption; Adolescent; Adult; Antibodies, Anti-Idiotypic; Autoantibodies; Biotin; Cohort Studies; Diabetes Mellitus, Type 1; Female; Glutamate Decarboxylase; Humans; Immunoassay; Male; Protein Binding; Research Design; Sensitivity and Specificity; Serologic Tests; Streptavidin | 2009 |
A biosensor based on photonic crystal surface waves with an independent registration of the liquid refractive index.
Topics: Biosensing Techniques; Biotin; Equipment Design; Equipment Failure Analysis; Nephelometry and Turbidimetry; Photons; Refractometry; Reproducibility of Results; Sensitivity and Specificity; Solutions; Streptavidin; Surface Plasmon Resonance | 2010 |
Detection of influenza A and B neutralizing antibodies in vaccinated ferrets and macaques using specific biotin-streptavidin conjugated antibodies.
Topics: Animals; Antibodies; Antibodies, Neutralizing; Antibodies, Viral; Biotin; Cell Line; Dogs; Ferrets; Fluorescent Dyes; Influenza A Virus, H1N1 Subtype; Influenza B virus; Influenza Vaccines; Macaca mulatta; Male; Neutralization Tests; Sensitivity and Specificity; Staining and Labeling; Streptavidin | 2010 |
Clinical impact of SPECT/CT with In-111 biotin on the management of patients with suspected spine infection.
Topics: Adult; Aged; Aged, 80 and over; Biotin; Female; Humans; Indium Radioisotopes; Male; Middle Aged; Osteomyelitis; Radiopharmaceuticals; Reproducibility of Results; Sensitivity and Specificity; Spinal Diseases; Subtraction Technique; Tomography, Emission-Computed, Single-Photon; Tomography, X-Ray Computed | 2010 |
A sensitive TLRH targeted imaging technique for ultrasonic molecular imaging.
Topics: Avidin; Biotin; Cellulose; Contrast Media; Microbubbles; Molecular Imaging; Phantoms, Imaging; Pressure; Sensitivity and Specificity; Signal Processing, Computer-Assisted; Transducers; Ultrasonography | 2010 |
Real-time biomolecular binding detection using a sensitive photonic crystal biosensor.
Topics: Antibodies; Binding Sites; Biosensing Techniques; Biotin; Biotinylation; Oligonucleotides; Photons; Protein Binding; Proteins; Sensitivity and Specificity; Streptavidin | 2010 |
Development of a sensitive biotin-avidin amplified enzyme-linked immunosorbent assay for the determination of ketamine in biological samples.
Topics: Animals; Antibodies; Antigen-Antibody Reactions; Avidin; Biotin; Cattle; Enzyme-Linked Immunosorbent Assay; Ketamine; Rabbits; Sensitivity and Specificity; Serum Albumin, Bovine | 2010 |
DNA-bridged bioconjugation of fluorescent quantum dots for highly sensitive microfluidic protein chips.
Topics: Binding Sites; Biomarkers, Tumor; Biotin; Carcinoembryonic Antigen; DNA; Enzyme-Linked Immunosorbent Assay; Fluorescence; Microfluidic Analytical Techniques; Microscopy, Atomic Force; Particle Size; Protein Array Analysis; Quantum Dots; Sensitivity and Specificity; Streptavidin | 2010 |
A high-throughput biotin-avidin-ELISA for studying expression of platelet membrane glycoproteins and its clinical application.
Topics: Adenosine Diphosphate; Antibodies, Monoclonal; Avidin; Biomarkers; Biotin; Cerebral Hemorrhage; Diabetes Mellitus; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Humans; Myocardial Infarction; Platelet Membrane Glycoproteins; Sensitivity and Specificity | 2010 |
A novel electrochemiluminescence strategy for ultrasensitive DNA assay using luminol functionalized gold nanoparticles multi-labeling and amplification of gold nanoparticles and biotin-streptavidin system.
Topics: Base Sequence; Biosensing Techniques; Biotin; DNA; Electrochemistry; Gold; Humans; Luminescent Measurements; Luminol; Nanoparticles; Sensitivity and Specificity; Serum; Streptavidin | 2010 |
A novel homogeneous Biotin-digoxigenin based assay for the detection of human anti-therapeutic antibodies in autoimmune serum.
Topics: Anti-Inflammatory Agents, Non-Steroidal; Antibodies, Anti-Idiotypic; Antibodies, Monoclonal; Arthritis, Rheumatoid; Biotin; Clinical Trials as Topic; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Female; Humans; Luminescent Measurements; Male; Sensitivity and Specificity | 2010 |
Ultrasensitive electrochemical detection of biotin using electrically addressable site-oriented antibody immobilization approach via aminophenyl boronic acid.
Topics: Animals; Antibodies, Immobilized; Biosensing Techniques; Biotin; Boronic Acids; Electrochemical Techniques; Gold; Graphite; Humans; Immunoassay; Liposomes; Metal Nanoparticles; Mice; Nanotechnology; Sensitivity and Specificity | 2010 |
A novel strategy for screening-out raw milk contaminated with Mycobacterium bovis on dairy farms by double-tagging PCR and electrochemical genosensing.
Topics: Animals; Bacteriological Techniques; Biotin; Digoxigenin; DNA Primers; DNA Transposable Elements; DNA, Bacterial; Electrochemical Techniques; Mass Screening; Milk; Mycobacterium bovis; Polymerase Chain Reaction; Sensitivity and Specificity; Staining and Labeling | 2010 |
Label-free optical biosensing using a horizontal air-slot SiNx microdisk resonator.
Topics: Biosensing Techniques; Biotin; Equipment Design; Hydrogen-Ion Concentration; Microscopy, Electron, Scanning; Models, Statistical; Normal Distribution; Optics and Photonics; Refractometry; Sensitivity and Specificity; Silicon Compounds; Staining and Labeling; Streptavidin | 2010 |
Enhancement of deoxyribonucleic acid microarray performance using post-hybridization signal amplification.
Topics: Avidin; Bacterial Proteins; Biotin; DNA, Bacterial; DNA, Viral; Electrochemistry; Francisella tularensis; Horseradish Peroxidase; Influenza A virus; Microelectrodes; Oligonucleotide Array Sequence Analysis; Oligonucleotide Probes; Sensitivity and Specificity | 2010 |
Clinical immunogenicity specificity assessments: a platform evaluation.
Topics: Antibodies; Antibodies, Monoclonal; Antibody Formation; Biotin; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Humans; Luminescent Measurements; Ruthenium; Sensitivity and Specificity | 2011 |
Covalent binding of streptavidin on gold magnetic nanoparticles for bead array fabrication.
Topics: Adsorption; Biotin; Gold; Immobilized Proteins; Magnetite Nanoparticles; Microspheres; Nanotechnology; Oligonucleotide Array Sequence Analysis; Oligonucleotides; Protein Stability; Sensitivity and Specificity; Streptavidin | 2010 |
Nitrosative protein oxidation is modulated during early endotoxemia.
Topics: Animals; Biotin; Blood Pressure; Cysteine; Endotoxemia; Escherichia coli; Heart; HEK293 Cells; Humans; Hypotension; Immunoblotting; In Vitro Techniques; Lipopolysaccharides; Mass Spectrometry; Metalloporphyrins; Mice; Mice, Inbred C57BL; Models, Animal; Nitric Oxide; Nitric Oxide Synthase; Oxidation-Reduction; Peroxynitrous Acid; Protein Processing, Post-Translational; Proteins; Rats; Rats, Wistar; S-Nitroso-N-Acetylpenicillamine; Sensitivity and Specificity; Sepsis; Signal Transduction; Telemetry; Tyrosine | 2011 |
Wide-field single metal nanoparticle spectroscopy for high throughput localized surface plasmon resonance sensing.
Topics: Biosensing Techniques; Biotin; Equipment Design; Metal Nanoparticles; Sensitivity and Specificity; Silver; Spectrum Analysis; Streptavidin; Surface Plasmon Resonance; Surface Properties | 2011 |
A nanoparticle-based sensor for visual detection of multiple mutations.
Topics: Adsorption; Antibodies; Biosensing Techniques; Biotin; DNA; DNA Mutational Analysis; DNA Primers; DNA Probes; Genotype; Gold; Metal Nanoparticles; Microscopy, Electron, Scanning; Microspheres; Nucleic Acid Hybridization; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Reagent Strips; Reproducibility of Results; Sensitivity and Specificity | 2011 |
Bead packing and release using flexible polydimethylsiloxane membrane for semi-continuous biosensing.
Topics: Biosensing Techniques; Biotin; Dimethylpolysiloxanes; Equipment Design; Fluoresceins; Humans; Immunoassay; Interleukin-6; Microfluidic Analytical Techniques; Sensitivity and Specificity; Streptavidin | 2011 |
Molecular detection of bacterial pathogens using microparticle enhanced double-stranded DNA probes.
Topics: Biological Assay; Biotin; DNA; DNA Probes; Escherichia coli; Fluorescent Dyes; Humans; Nucleic Acid Hybridization; Pseudomonas; RNA, Ribosomal, 16S; Sensitivity and Specificity; Staphylococcus saprophyticus; Streptavidin; Urinary Tract Infections | 2011 |
Ultrasensitive detection of ferritin in human serum by Western blotting based on quantum dots-labeled avidin-biotin system.
Topics: Avidin; Biotin; Blotting, Western; Carcinoma, Hepatocellular; Ferritins; Humans; Liver Neoplasms; Quantum Dots; Sensitivity and Specificity; Thalassemia | 2011 |
Ultrasensitive electrochemical detection of Bacillus thuringiensis transgenic sequence based on in situ Ag nanoparticles aggregates induced by biotin-streptavidin system.
Topics: Bacillus thuringiensis; Biosensing Techniques; Biotin; DNA, Bacterial; Electrochemical Techniques; Metal Nanoparticles; Sensitivity and Specificity; Silver; Streptavidin | 2011 |
Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection.
Topics: Animals; Biotin; Cattle; DNA; Fluorescent Dyes; Nanoparticles; Oligonucleotide Array Sequence Analysis; Sensitivity and Specificity; Serum Albumin, Bovine; Silicon Dioxide; Streptavidin | 2011 |
Measurement of 3-hydroxyisovaleric acid in urine from marginally biotin-deficient humans by UPLC-MS/MS.
Topics: Adult; Biomarkers; Biotin; Biotinidase Deficiency; Calibration; Chromatography, Liquid; Creatinine; Egg White; Female; Humans; Male; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Tandem Mass Spectrometry; Valerates | 2011 |
Label-free THz sensing of living body-related molecular binding using a metallic mesh.
Topics: Avidin; Biotin; Carbohydrate Metabolism; Lectins; Metals; Polyvinyls; Sensitivity and Specificity; Terahertz Spectroscopy | 2011 |
Enhancement of the detection limit for lateral flow immunoassays: evaluation and comparison of bioconjugates.
Topics: Antibodies, Monoclonal; Biotin; Dengue; Dengue Virus; Early Diagnosis; Gold Colloid; Immunoassay; Limit of Detection; Nanoparticles; Particle Size; Polystyrenes; Sensitivity and Specificity; Silver; Soot; Streptavidin; Viral Nonstructural Proteins | 2012 |
Fluorescence signal enhancement of polydiacetylene vesicle stacks.
Topics: Avidin; Biosensing Techniques; Biotin; Fatty Acids, Unsaturated; Fluorescent Dyes; Liposomes; Polyacetylene Polymer; Polymers; Polyynes; Sensitivity and Specificity; Spectrometry, Fluorescence; Spectroscopy, Fourier Transform Infrared | 2011 |
Bioconjugation strategies for microtoroidal optical resonators.
Topics: Biosensing Techniques; Biotin; Optical Devices; Sensitivity and Specificity; Silicon Dioxide; Staining and Labeling; Surface Plasmon Resonance | 2010 |
Detection of single-nucleotide polymorphisms with novel leaky surface acoustic wave biosensors, DNA ligation and enzymatic signal amplification.
Topics: 3,3'-Diaminobenzidine; Bacterial Proteins; Biosensing Techniques; Biotin; DNA; Horseradish Peroxidase; Humans; Hydrogen-Ion Concentration; Polymorphism, Single Nucleotide; Sensitivity and Specificity; Sound | 2012 |
Whispering gallery modes in standard optical fibres for fibre profiling measurements and sensing of unlabelled chemical species.
Topics: Air; Biotin; Chemistry Techniques, Analytical; Models, Theoretical; Optical Fibers; Refractometry; Sensitivity and Specificity; Streptavidin; Water | 2010 |
Nanobiocomposite platform based on polyaniline-iron oxide-carbon nanotubes for bacterial detection.
Topics: Aniline Compounds; Avidin; Biosensing Techniques; Biotin; Electrochemistry; Ferric Compounds; Gonorrhea; Humans; Male; Microelectrodes; Microscopy, Electron, Scanning; Nanocomposites; Nanotubes, Carbon; Neisseria gonorrhoeae; Nucleic Acid Hybridization; Sensitivity and Specificity; Spectroscopy, Fourier Transform Infrared; Tin Compounds; X-Ray Diffraction | 2012 |
Electrochemical determination of microRNA-21 based on graphene, LNA integrated molecular beacon, AuNPs and biotin multifunctional bio bar codes and enzymatic assay system.
Topics: Biosensing Techniques; Biotin; Electrochemical Techniques; Electronic Data Processing; Gold; Graphite; Horseradish Peroxidase; Humans; Metal Nanoparticles; MicroRNAs; Oligonucleotides; Sensitivity and Specificity | 2012 |
A bispecific antibody based assay shows potential for detecting tuberculosis in resource constrained laboratory settings.
Topics: Animals; Antibodies, Bispecific; Antibodies, Monoclonal; Antigens, Bacterial; Biotin; Blotting, Western; Cattle; Cell Line; Clinical Laboratory Techniques; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Health Resources; Humans; Limit of Detection; Lipopolysaccharides; Mycobacterium tuberculosis; Rabbits; Rats; Sensitivity and Specificity; Serum Albumin, Bovine; Staining and Labeling; Tuberculosis | 2012 |
Sensitive detection of small molecules by competitive immunomagnetic-proximity ligation assay.
Topics: Animals; Antibodies, Monoclonal; Biotin; Cattle; Clenbuterol; Immunoassay; Magnetics; Phenethylamines; Sensitivity and Specificity; Serum Albumin, Bovine; Streptavidin | 2012 |
Nephrogenic adenoma: an immunohistochemical analysis using biotin-free methods.
Topics: Adenoma; Aged; Aged, 80 and over; Artifacts; Biotin; Cadherins; Cell Transformation, Neoplastic; Diagnosis, Differential; Female; Humans; Immunohistochemistry; Kidney Neoplasms; Kidney Tubules, Distal; Male; Metaplasia; Middle Aged; Neprilysin; Racemases and Epimerases; Sensitivity and Specificity; Stem Cell Factor; Transcription Factors; Tumor Suppressor Proteins; Urothelium | 2012 |
Ultrasensitive aptamer-based bio bar code immunomagnetic separation and electrochemiluminescence method for the detection of protein.
Topics: 2,2'-Dipyridyl; Animals; Aptamers, Nucleotide; Becaplermin; Biosensing Techniques; Biotin; Cattle; Coordination Complexes; Electrochemical Techniques; Equipment Design; Gold; Humans; Immunoassay; Limit of Detection; Luminescent Measurements; Metal Nanoparticles; Proto-Oncogene Proteins c-sis; Sensitivity and Specificity; Streptavidin | 2012 |
Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin.
Topics: Animals; Bacterial Proteins; Biotin; Blotting, Western; Cattle; Cell Line; Horseradish Peroxidase; Humans; Indicators and Reagents; Luminescent Measurements; Phosphopeptides; Phosphoproteins; Phosphorylation; Protein Array Analysis; Proteomics; Pyridines; Sensitivity and Specificity | 2012 |
Purification of histidine-tagged aequorin with a reactive cysteine residue for chemical conjugations and its application for bioluminescent sandwich immunoassays.
Topics: Aequorin; alpha-Fetoproteins; Biotin; Cysteine; Histidine; Immunoassay; Luminescent Measurements; Maleimides; Models, Chemical; Recombinant Fusion Proteins; Sensitivity and Specificity; Streptavidin | 2012 |
Immobilisation of quantum dots by bio-orthogonal PCR amplification and labelling for direct gene detection and quantitation.
Topics: Antibodies; Biological Assay; Biotin; Digoxigenin; DNA; DNA Primers; Histidine Decarboxylase; Humans; Leukocytes; Plasmids; Point-of-Care Systems; Polymerase Chain Reaction; Quantum Dots; Sensitivity and Specificity; Spectrometry, Fluorescence; Staining and Labeling; Streptavidin | 2012 |
Mitochondrial single nucleotide polymorphism genotyping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using cleavable biotinylated dideoxynucleotides.
Topics: Base Sequence; Biotin; Biotinylation; Cell Line; Dideoxynucleotides; DNA Fingerprinting; DNA, Mitochondrial; Humans; MERRF Syndrome; Mitochondria; Molecular Sequence Data; Mutation; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Purines; Pyrimidines; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Streptavidin | 2012 |
A new method for the capture of surface proteins in Plasmodium falciparum parasitized erythrocyte.
Topics: Adult; Biotin; Erythrocytes; Female; Humans; Male; Mass Spectrometry; Membrane Proteins; Parasitology; Plasmodium falciparum; Protozoan Proteins; Sensitivity and Specificity; Staining and Labeling | 2012 |
Conditions of polymerase chain reaction amplification by magnetic enrichment and nanoscale detection sensitivity.
Topics: Biotin; DNA; Ferric Compounds; Humans; Magnetics; Nanoparticles; Nanotechnology; Nucleic Acid Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity; Temperature | 2012 |
[Establishment of BA-ELISA method for detecting CEA in human sera].
Topics: Animals; Avidin; Biotin; Breast Neoplasms; Carcinoembryonic Antigen; Enzyme-Linked Immunosorbent Assay; Female; Humans; Rabbits; Reference Values; Reproducibility of Results; Sensitivity and Specificity | 2012 |
Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies.
Topics: Animals; Antibodies, Monoclonal; Aryl Hydrocarbon Hydroxylases; Avidin; Biotin; Breast; Breast Neoplasms; Cytochrome P-450 CYP1A1; Cytochrome P-450 CYP1B1; Enzyme-Linked Immunosorbent Assay; Female; Histocytochemistry; Humans; Mass Spectrometry; Mice; Molecular Imaging; Rats; Recombinant Proteins; Sensitivity and Specificity; Single-Chain Antibodies | 2012 |
Strategies to estimate and improve drug tolerance in anti-drug antibody assays.
Topics: Antibodies, Anti-Idiotypic; Antibodies, Monoclonal; Antigen-Antibody Complex; Biotin; Drug Tolerance; Enzyme-Linked Immunosorbent Assay; Humans; Immunity, Humoral; Recombinant Proteins; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Serine Proteases; Streptavidin | 2012 |
Single vesicle analysis reveals nanoscale membrane curvature selective pore formation in lipid membranes by an antiviral α-helical peptide.
Topics: Antiviral Agents; Biotin; Cell Membrane; Fluoresceins; Kinetics; Lipids; Membrane Lipids; Nanoparticles; Nanotechnology; Peptides; Phosphatidylcholines; Protein Structure, Secondary; Rhodamines; Sensitivity and Specificity; Surface Plasmon Resonance; Surface Properties | 2012 |
A fast and sensitive quantitative lateral flow immunoassay for Cry1Ab based on a novel signal amplification conjugate.
Topics: Antibodies; Biotin; Fluorescent Dyes; Immunoassay; Limit of Detection; Polylysine; Sensitivity and Specificity | 2012 |
Electrochemiluminescent disposable cholesterol biosensor based on avidin-biotin assembling with the electroformed luminescent conducting polymer poly(luminol-biotinylated pyrrole).
Topics: Avidin; Biosensing Techniques; Biotin; Cholesterol; Click Chemistry; Electrochemical Techniques; Humans; Hydrogen-Ion Concentration; Luminescent Measurements; Luminol; Models, Molecular; Molecular Structure; Polymers; Pyrroles; Sensitivity and Specificity | 2012 |
Immunohistochemical detection of disease-associated prion protein in the peripheral nervous system in experimental H-type bovine spongiform encephalopathy.
Topics: Adrenal Glands; Animals; Biotin; Brain; Cattle; Encephalopathy, Bovine Spongiform; Female; Fluorescent Antibody Technique; Fluorescent Antibody Technique, Indirect; Immunohistochemistry; Peripheral Nervous System; PrPSc Proteins; Schwann Cells; Sensitivity and Specificity; Tyramine | 2013 |
Part per trillion label-free electronic bioanalytical detection.
Topics: Adsorption; Antibodies; Biotin; Electrochemical Techniques; Fluorescent Dyes; Immobilized Proteins; Kinetics; Microfluidic Analytical Techniques; Reagent Strips; Sensitivity and Specificity; Streptavidin; Transistors, Electronic | 2013 |
A biotin capture assay for oligosaccharyltransferase.
Topics: Biological Assay; Biotin; Chromatography, High Pressure Liquid; Hexosyltransferases; Kinetics; Membrane Proteins; Peptides; Sensitivity and Specificity; Transferases | 2002 |
A label-free optical technique for detecting small molecule interactions.
Topics: Avidin; Biosensing Techniques; Biotin; Caspase 3; Caspases; Colorimetry; Enzyme Activation; Enzymes, Immobilized; Macromolecular Substances; Optics and Photonics; Protein Binding; Protein Interaction Mapping; Reproducibility of Results; Sensitivity and Specificity; Spectrum Analysis; Substrate Specificity; Titrimetry | 2002 |
A nanoscale optical biosensor: sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles.
Topics: Biosensing Techniques; Biotin; Colloids; Gold; Kinetics; Nanotechnology; Protein Binding; Sensitivity and Specificity; Serum Albumin, Bovine; Silver; Streptavidin; Surface Plasmon Resonance; Surface Properties | 2002 |
Diffraction-based assay for detecting multiple analytes.
Topics: Animals; Antibody Specificity; Avidin; Biosensing Techniques; Biotin; Fluorescein; Immunoglobulin G; Light; Mice; Molecular Probes; Protein Binding; Rabbits; Sensitivity and Specificity; Solutions; Substrate Specificity | 2002 |
Non-radioactive in situ detection of mRNA in ES cell-derived cardiomyocytes and in the developing heart.
Topics: Animals; Atrial Natriuretic Factor; Biotin; Cardiac Myosins; Heart; In Situ Hybridization; Mice; Myoblasts, Cardiac; Myocardium; Myocytes, Cardiac; Myosin Light Chains; RNA, Messenger; Sensitivity and Specificity; Troponin I; Tyramine | 2002 |
SPARK--a novel method to monitor ribosomal peptidyl transferase activity.
Topics: Anti-Bacterial Agents; Biotin; Enzyme Activation; Immunoassay; Kinetics; Peptidyl Transferases; Protein Subunits; Puromycin; Ribosomal Proteins; Ribosomes; RNA, Ribosomal; RNA, Transfer, Amino Acid-Specific; Scintillation Counting; Sensitivity and Specificity; Thermus | 2002 |
DNA sequencing with solid-phase-capturable dideoxynucleotides and energy transfer primers.
Topics: Base Sequence; Biotin; Biotinylation; Dideoxynucleosides; DNA; DNA Primers; Energy Transfer; Fluorescence Resonance Energy Transfer; Microspheres; Molecular Sequence Data; Sensitivity and Specificity; Sequence Analysis, DNA; Streptavidin; Templates, Genetic | 2002 |
Development of nonradioactive microtiter plate assays for nuclease activity.
Topics: Aurintricarboxylic Acid; Base Sequence; Biotin; Calibration; Deoxyribonuclease I; Digoxigenin; DNA; Edetic Acid; Enzyme-Linked Immunosorbent Assay; Microspheres; Oligonucleotides; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Single-Strand Specific DNA and RNA Endonucleases; Streptavidin; Time Factors | 2002 |
Amperometric DNA sensor using the pyrroquinoline quinone glucose dehydrogenase-avidin conjugate.
Topics: Avidin; Bacterial Proteins; Biosensing Techniques; Biotin; Calibration; Carbon; DNA; DNA Probes; DNA, Bacterial; Electrochemistry; Electrodes; Enzymes, Immobilized; Equipment Design; Glucose; Glucose Dehydrogenases; Materials Testing; Reference Values; Reproducibility of Results; Salmonella; Sensitivity and Specificity | 2002 |
Label-free bioaffinity detection using terahertz technology.
Topics: Affinity Labels; Avidin; Biosensing Techniques; Biotin; Electromagnetic Phenomena; Equipment Design; Macromolecular Substances; Membranes, Artificial; Microwaves; Protein Binding; Refractometry; Sensitivity and Specificity; Spectrum Analysis; Surface Properties | 2002 |
An innovative strategy for immobilization of receptor proteins on to an optical fiber by use of poly(pyrrole-biotin).
Topics: Biotin; Enzyme-Linked Immunosorbent Assay; Fiber Optic Technology; Luminescent Measurements; Microscopy, Fluorescence; Optical Fibers; Polymers; Pyrroles; Sensitivity and Specificity; Spectroscopy, Fourier Transform Infrared | 2002 |
Biotinyl-tyramide-based in situ hybridization signal patterns distinguish human papillomavirus type and grade of cervical intraepithelial neoplasia.
Topics: Biotin; DNA, Viral; Female; Humans; In Situ Hybridization; Papillomaviridae; Papillomavirus Infections; Polymerase Chain Reaction; Sensitivity and Specificity; Tumor Cells, Cultured; Tumor Virus Infections; Tyramine; Uterine Cervical Dysplasia; Uterine Cervical Neoplasms | 2002 |
Immunodetection and molecular forms of plasma vascular endothelial growth factor-C.
Topics: Adenocarcinoma; Animals; Avidin; Biomarkers, Tumor; Biotin; Colorectal Neoplasms; Cross Reactions; Endothelial Growth Factors; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique, Indirect; Goats; Humans; Luminescent Measurements; Neoplasm Proteins; Protein Isoforms; Rabbits; Recombinant Proteins; Reproducibility of Results; Sensitivity and Specificity; Species Specificity; Vascular Endothelial Growth Factor C | 2003 |
[Studies on detection methods of two DNA probes in Mycobacterium tuberculosis].
Topics: Biotin; DNA Probes; DNA, Bacterial; Mycobacterium tuberculosis; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity; Temperature | 2000 |
Protein patterning on silicon-based surface using background hydrophobic thin film.
Topics: Biosensing Techniques; Biotin; Coated Materials, Biocompatible; Feasibility Studies; Hydrophobic and Hydrophilic Interactions; Membranes, Artificial; Protein Array Analysis; Protein Binding; Reproducibility of Results; Sensitivity and Specificity; Silicon; Spectrometry, Fluorescence; Streptavidin | 2003 |
High sensitivity detection of molecular recognition using magnetically labelled biomolecules and magnetoresistive sensors.
Topics: Antigen-Antibody Complex; Biosensing Techniques; Biotin; Equipment Design; Magnetics; Microchemistry; Microspheres; Nanotechnology; Reproducibility of Results; Sensitivity and Specificity; Spin Labels; Staining and Labeling; Streptavidin; Transducers | 2003 |
Characterization of 11-dehydro-thromboxane B2 recombinant antibody obtained by phage display technology.
Topics: Amino Acid Sequence; Animals; Antibodies, Monoclonal; Antibody Affinity; Biotin; Biotinylation; DNA, Complementary; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Female; Genetic Vectors; Immunoglobulins; Kinetics; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Peptide Library; Plasmids; Prostaglandins; Sensitivity and Specificity; Thromboxane B2 | 2003 |
Use of a sensitive EnVision +-based detection system for Western blotting: avoidance of streptavidin binding to endogenous biotin and biotin-containing proteins in kidney and other tissues.
Topics: Biotin; Blotting, Western; Kidney; Proteins; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2003 |
A sensitive non-isotopic assay for GAD65 autoantibodies.
Topics: Autoantibodies; Autoimmune Diseases; Biotin; Diabetes Mellitus, Type 1; Diabetes Mellitus, Type 2; Enzyme-Linked Immunosorbent Assay; Fungal Proteins; Glutamate Decarboxylase; Humans; Immunoassay; Iodine Radioisotopes; Isoenzymes; Reproducibility of Results; Sensitivity and Specificity; Yeasts | 2003 |
In situ hybridization in neural tissues.
Topics: Animals; Biotin; Central Nervous System; Digoxigenin; Fixatives; Humans; Image Processing, Computer-Assisted; In Situ Hybridization; Ribonuclease, Pancreatic; RNA Probes; RNA, Messenger; Sensitivity and Specificity; Staining and Labeling; Temperature; Transcription, Genetic | 2003 |
Miniaturizable homogenous time-resolved fluorescence assay for carboxypeptidase B activity.
Topics: Aminocaproates; Animals; Antibodies, Monoclonal; Biotin; Carboxypeptidase B; Enzyme Inhibitors; Europium; Fluorescence Resonance Energy Transfer; Fluorescent Antibody Technique; Miniaturization; Oligopeptides; Phycocyanin; Sensitivity and Specificity; Streptavidin; Swine | 2003 |
Plasma 11beta-hydroxy-4-androstene-3,17-dione: comparison of a time-resolved fluoroimmunoassay using a biotinylated tracer with a radioimmunoassay using a tritiated tracer.
Topics: Androstenedione; Biotin; Dose-Response Relationship, Drug; Europium; Female; Fluoroimmunoassay; Humans; Radioimmunoassay; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2003 |
FRET or no FRET: a quantitative comparison.
Topics: Algorithms; Artifacts; Biotin; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Monte Carlo Method; Protein Binding; Quality Control; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2003 |
Development and application of a pig IL-8 ELISA detection system.
Topics: Amniotic Fluid; Animals; Antibodies, Monoclonal; Antibody Specificity; Biotin; Cells, Cultured; Cytokines; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Fetus; Interleukin-8; Leukocytes, Mononuclear; Mice; Mice, Inbred BALB C; Recombinant Proteins; Sensitivity and Specificity; Streptavidin; Swine | 2003 |
Imaging tumor angiogenesis with contrast ultrasound and microbubbles targeted to alpha(v)beta3.
Topics: Animals; Biotin; Blood Flow Velocity; Brain Neoplasms; Contrast Media; Glioblastoma; Humans; Immunohistochemistry; Integrin alphaVbeta3; Intercellular Signaling Peptides and Proteins; Microcirculation; Neoplasm Transplantation; Neovascularization, Pathologic; Peptides; Predictive Value of Tests; Rats; Rats, Nude; Sensitivity and Specificity; Transplantation, Heterologous; Ultrasonography | 2003 |
Use of SAM2 biotin capture membrane in microarrayed compound screening (muARCS) format for nucleic acid polymerization assays.
Topics: Alkynes; Anti-HIV Agents; Benzoxazines; Biotin; Biotinylation; Cyclopropanes; DNA; Dose-Response Relationship, Drug; HIV Reverse Transcriptase; Inhibitory Concentration 50; Nevirapine; Nucleic Acids; Oligonucleotide Array Sequence Analysis; Oxazines; Plasmids; RNA; RNA-Directed DNA Polymerase; Sensitivity and Specificity; Templates, Genetic | 2003 |
Molecular engineering of biotin-glutamic acid decarboxylase 65 fusion protein (Biotin-GAD65) for non-radioactive GAD65 antibody assay.
Topics: Antibodies; Antigen-Antibody Complex; Biosensing Techniques; Biotin; Enzyme-Linked Immunosorbent Assay; Glutamate Decarboxylase; Isoenzymes; Protein Binding; Protein Engineering; Radioimmunoassay; Recombinant Fusion Proteins; Reproducibility of Results; Sensitivity and Specificity | 2003 |
In vivo evaluation of pretargeted 64Cu for tumor imaging and therapy.
Topics: Animals; Antibodies, Monoclonal; Biomarkers, Tumor; Biotin; Colorectal Neoplasms; Copper Radioisotopes; Drug Delivery Systems; Humans; Immunoconjugates; Metabolic Clearance Rate; Mice; Mice, Nude; Organ Specificity; Organometallic Compounds; Radioimmunodetection; Radioimmunotherapy; Radiopharmaceuticals; Reproducibility of Results; Sensitivity and Specificity; Tissue Distribution | 2003 |
Immunoradiometric assay (IRMA) for human follicle stimulating hormone (FSH) and luteinizing hormone (LH) using common avidin solid phase.
Topics: Antibodies; Avidin; Biotin; Follicle Stimulating Hormone; Humans; Immunoradiometric Assay; Luteinizing Hormone; Molecular Probes; Sensitivity and Specificity | 2003 |
On the specificity of biotin in the metabolism of propionibacteria.
Topics: Biochemical Phenomena; Biotin; Humans; Propionibacterium; Sensitivity and Specificity | 1958 |
Rapid and sensitive identification of epitope-containing peptides by direct matrix-assisted laser desorption/ionization tandem mass spectrometry of peptides affinity-bound to antibody beads.
Topics: Amino Acid Sequence; Antibodies; Antibodies, Phospho-Specific; Antibody Specificity; Avidin; Biotin; Epitopes; Microspheres; Peptides; Phosphotyrosine; Protein Binding; Proto-Oncogene Proteins c-myc; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time Factors | 2003 |
Characterization and application of Raman labels for confocal Raman microspectroscopic detection of cellular proteins in single cells.
Topics: Angiotensin II; Animals; Arachidonate 12-Lipoxygenase; Benzoxazines; Biotin; Cattle; Cells, Cultured; Citrates; Colloids; Coloring Agents; Coronary Vessels; Cyclooxygenase 1; Endothelium, Vascular; Fluorescent Antibody Technique; Isoenzymes; Microscopy, Confocal; Oxazines; p-Dimethylaminoazobenzene; Prostaglandin-Endoperoxide Synthases; Proteins; Receptors, Angiotensin; Sensitivity and Specificity; Silver Compounds; Spectrum Analysis, Raman; Zona Glomerulosa | 2003 |
A new quantitative optical biosensor for protein characterisation.
Topics: Antibodies; Biosensing Techniques; Biotin; Equipment Design; Equipment Failure Analysis; Humans; Interferometry; Optics and Photonics; Proteins; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2003 |
Development of a sensitive microarray immunoassay and comparison with standard enzyme-linked immunoassay for cytokine analysis.
Topics: Antibodies; Biotin; Collodion; Cytokines; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Humans; Immunoenzyme Techniques; Inflammation; Protein Array Analysis; Proteome; Proteomics; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2004 |
Submicrometer spatial resolution of metal-enhanced fluorescence.
Topics: Avidin; Biotin; Fluorescein; Fluorescence; Metals; Microscopy, Confocal; Nanotechnology; Proteins; Sensitivity and Specificity; Serum Albumin, Bovine; Silver | 2003 |
Enhanced protein profiling arrays with ELISA-based amplification for high-throughput molecular changes of tumor patients' plasma.
Topics: Adolescent; Adult; Aged; Aged, 80 and over; Biotin; Cell Line, Tumor; Cluster Analysis; Enzyme-Linked Immunosorbent Assay; Epidermal Growth Factor; Female; Genital Neoplasms, Female; Humans; Immunoglobulin G; Interleukin-8; Luminescent Measurements; Microscopy, Fluorescence; Middle Aged; Multigene Family; Neoplasms; Neovascularization, Pathologic; Oligonucleotide Array Sequence Analysis; Platelet-Derived Growth Factor; Protein Array Analysis; Ribonuclease, Pancreatic; Sensitivity and Specificity; Tissue Distribution; Vascular Endothelial Growth Factor A | 2004 |
A monolithic silicon optoelectronic transducer as a real-time affinity biosensor.
Topics: Adsorption; Animals; Biosensing Techniques; Biotin; Cattle; Equipment Design; Gold; Nanotechnology; Optics and Photonics; Sensitivity and Specificity; Serum Albumin, Bovine; Silicon; Silver; Streptavidin; Time Factors; Transducers | 2004 |
Recovering the true targets of specific ligands by virtual screening of the protein data bank.
Topics: Algorithms; Amino Acid Sequence; Binding Sites; Biotin; Computational Biology; Computer Simulation; Crystallography, X-Ray; Databases, Protein; Drug Evaluation, Preclinical; Ligands; Methotrexate; Molecular Sequence Data; Molecular Weight; Proteins; Purine Nucleosides; Ribonucleosides; Sensitivity and Specificity; Software; Substrate Specificity; Tamoxifen | 2004 |
A protein microarray ELISA for screening biological fluids.
Topics: Amides; Antibodies; Antibody Affinity; Biotin; Body Fluids; Enzyme-Linked Immunosorbent Assay; Epitopes; Protein Array Analysis; Proteins; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2004 |
Cytokine protein arrays.
Topics: Animals; Antibodies; Biotin; Cytokines; Enzyme-Linked Immunosorbent Assay; Humans; Protein Array Analysis; Reference Standards; Sensitivity and Specificity; Streptavidin | 2004 |
Optimization of in situ hybridization assay using non-radioactive DNA probes for the detection of canine herpesvirus (CHV) in paraffin-embedded sections.
Topics: Animals; Biotin; Cell Line; Digoxigenin; DNA Probes; DNA, Viral; Dog Diseases; Dogs; Herpesviridae Infections; Herpesvirus 1, Canid; In Situ Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity | 2004 |
Two-color, rolling-circle amplification on antibody microarrays for sensitive, multiplexed serum-protein measurements.
Topics: Antibodies; Biotin; Blood Proteins; Color; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Humans; Immunoassay; Liver Cirrhosis; Liver Neoplasms; Protein Array Analysis; Reproducibility of Results; Sensitivity and Specificity | 2004 |
Direct detection of Mycobacterium tuberculosis in clinical specimens using single-tube biotinylated nested polymerase chain reaction-enzyme linked immunoassay (PCR-ELISA).
Topics: Bacteriological Techniques; Biotin; Enzyme-Linked Immunosorbent Assay; Humans; Mycobacterium tuberculosis; Polymerase Chain Reaction; Sensitivity and Specificity; Tuberculosis | 2004 |
Construction of a dual chain pseudotetrameric chicken avidin by combining two circularly permuted avidins.
Topics: Amino Acid Sequence; Animals; Avidin; Binding Sites; Biotin; Chickens; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Endopeptidase K; Glycine; Kinetics; Models, Molecular; Molecular Sequence Data; Mutation; Peptides; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Sensitivity and Specificity; Serine | 2004 |
Rapid detection of mutations in Wilson disease gene ATP7B by DNA strip technology.
Topics: Adenosine Triphosphatases; Alkaline Phosphatase; Bacterial Proteins; Biotin; Biotinylation; Cation Transport Proteins; Copper-Transporting ATPases; DNA; DNA Mutational Analysis; DNA Primers; Genotype; Hepatolenticular Degeneration; Heterozygote; Homozygote; Humans; Nucleic Acid Hybridization; Predictive Value of Tests; Reagent Strips; Sensitivity and Specificity | 2004 |
Spontaneous integration of transmembrane peptides into a bacterial magnetic particle membrane and its application to display of useful proteins.
Topics: Antimicrobial Cationic Peptides; Bacterial Proteins; Biotin; Hydrogen-Ion Concentration; Magnetics; Membrane Fusion; Membrane Proteins; Membranes, Artificial; Peptide Fragments; Peptides; Sensitivity and Specificity; Sodium Chloride; Streptavidin; Surface Properties | 2004 |
Development of a highly sensitive and specific new testosterone time-resolved fluoroimmunoassay in human serum.
Topics: Biotin; Female; Fluorescent Antibody Technique; Fluoroimmunoassay; Humans; Male; Radioimmunoassay; Sensitivity and Specificity; Testosterone; Time Factors | 2004 |
DNA-directed protein immobilization on mixed self-assembled monolayers via a streptavidin bridge.
Topics: Antibodies; Antigen-Antibody Reactions; Biosensing Techniques; Biotin; Chorionic Gonadotropin; DNA; Humans; Immobilization; Sensitivity and Specificity; Streptavidin; Surface Plasmon Resonance; Surface Properties | 2004 |
Immobilization of the enzyme beta-lactamase on biotin-derivatized poly(L-lysine)-g-poly(ethylene glycol)-coated sensor chips: a study on oriented attachment and surface activity by enzyme kinetics and in situ optical sensing.
Topics: Adsorption; beta-Lactamases; Biosensing Techniques; Biotin; Enzymes, Immobilized; Kinetics; Niobium; Oxides; Particle Size; Polyethylene Glycols; Polylysine; Protein Conformation; Protein Structure, Secondary; Sensitivity and Specificity; Surface Properties; Time Factors | 2004 |
Development of a functionalized xenon biosensor.
Topics: Avidin; Biosensing Techniques; Biotin; Nuclear Magnetic Resonance, Biomolecular; Sensitivity and Specificity; Stereoisomerism; Xenon; Xenon Isotopes | 2004 |
Enzymatic genosensor on streptavidin-modified screen-printed carbon electrodes.
Topics: Adsorption; Alkaline Phosphatase; Biosensing Techniques; Biotin; Carbon; DNA, Viral; Electrochemistry; Electrodes; Equipment Design; Indoles; Oligonucleotide Probes; Sensitivity and Specificity; Streptavidin; Surface Properties | 2004 |
Micropatterning of DNA-tagged vesicles.
Topics: Biotin; Cholesterol; DNA, Complementary; Liposomes; Microarray Analysis; Microscopy, Fluorescence; Nucleic Acid Hybridization; Osmolar Concentration; Sensitivity and Specificity | 2004 |
Label-free molecular interaction determinations with nanoscale interferometry.
Topics: Biotin; Dimethylpolysiloxanes; Immunoglobulin G; Microscopy, Atomic Force; Microscopy, Interference; Nanotechnology; Protein Array Analysis; Protein Binding; Proteins; Sensitivity and Specificity; Silicones; Staphylococcal Protein A; Streptavidin | 2004 |
Development and evaluation of a new ELISA for the detection and quantification of antierythropoietin antibodies in human sera.
Topics: Animals; Antibodies; Biotin; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Erythropoietin; Humans; Lupus Erythematosus, Systemic; Peroxidase; Rabbits; Recombinant Proteins; Red-Cell Aplasia, Pure; Reference Standards; Reference Values; Renal Dialysis; Reproducibility of Results; Sensitivity and Specificity; Sjogren's Syndrome; Streptavidin | 2004 |
Immunoassay of total prostate-specific antigen using europium(III) nanoparticle labels and streptavidin-biotin technology.
Topics: Antibodies, Monoclonal; Biotin; Epitopes; Europium; Fluorescent Antibody Technique; Humans; Immunosorbent Techniques; Male; Microspheres; Prostate-Specific Antigen; Reference Standards; Reference Values; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2004 |
Widespread sulfenic acid formation in tissues in response to hydrogen peroxide.
Topics: Animals; Arsenites; Biochemistry; Biotin; Blotting, Western; Cyclohexanones; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Hydrogen Peroxide; Male; Maleimides; Mass Spectrometry; Models, Biological; Myocardium; Oxidation-Reduction; Oxidative Stress; Peptides; Perfusion; Protein Processing, Post-Translational; Rats; Rats, Wistar; Sensitivity and Specificity; Signal Transduction; Sulfenic Acids; Sulfhydryl Compounds | 2004 |
Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads.
Topics: Avidin; Binding Sites; Biotin; Magnetics; Micromanipulation; Microspheres; Physical Stimulation; Protein Binding; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Stress, Mechanical | 2005 |
Evaluation on the use of beta-lactamase and aminoglycoside modifying enzyme gene sequences as markers for the early detection of antibiotic resistance profile of Pseudomonas aeruginosa.
Topics: Amikacin; Aminoglycosides; Anti-Bacterial Agents; Anti-Infective Agents; beta-Lactamases; Biomarkers; Biotin; Blotting, Southern; Cross Infection; DNA; DNA Primers; Dose-Response Relationship, Drug; Drug Resistance, Bacterial; Electrophoresis, Agar Gel; Gentamicins; Humans; Microbial Sensitivity Tests; Nucleic Acid Hybridization; Penicillins; Polymerase Chain Reaction; Pseudomonas aeruginosa; Pseudomonas Infections; Sensitivity and Specificity; Sequence Analysis, DNA; Streptomycin; Tobramycin | 2004 |
Selective detection of 2-deoxyribonolactone in DNA.
Topics: Autoradiography; Biotin; Cysteine; DNA; DNA Damage; Gas Chromatography-Mass Spectrometry; Horseradish Peroxidase; Oxidation-Reduction; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sugar Acids | 2005 |
An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques.
Topics: Avidin; Biotin; Carbon; Chromatography, High Pressure Liquid; Drug Interactions; Electrochemistry; Electrodes; Reproducibility of Results; Sensitivity and Specificity | 2005 |
Development and validation of a simple, sensitive, second antibody format enzyme immunoassay (EIA) for LH determination in mithun (Bos frontalis) plasma.
Topics: Animals; Antibody Formation; Biotin; Cattle; Horseradish Peroxidase; Immunoenzyme Techniques; Luteinizing Hormone; Reproducibility of Results; Ruminants; Sensitivity and Specificity; Streptavidin | 2005 |
A pulsed potential waveform displaying enhanced detection capabilities towards sulfur-containing compounds at a gold working electrode.
Topics: Acetylcysteine; Biotin; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Cysteine; Cystine; Electrochemistry; Electrodes; Glutathione; Gold; Homocysteine; Methionine; Oxidation-Reduction; Reproducibility of Results; Sensitivity and Specificity; Sulfur Compounds; Thioctic Acid | 2005 |
Direct fluorescent staining and analysis of proteins on microspheres using CBQCA.
Topics: Animals; Avidin; Benzoates; Biotin; Cattle; Flow Cytometry; Fluorescent Antibody Technique, Direct; Fluorescent Dyes; Immunoglobulin G; Kinetics; Ligands; Microspheres; Nerve Tissue Proteins; Proteins; Quinolines; Sensitivity and Specificity; Staining and Labeling; Surface Properties; Time Factors | 2005 |
A new simplified catalyzed signal amplification system for minimizing non-specific staining in tissues with supersensitive immunohistochemistry.
Topics: Appendix; Biotin; Carcinoid Tumor; Carcinoma, Hepatocellular; Carcinoma, Squamous Cell; Caseins; Fluorescein-5-isothiocyanate; Fluorescent Dyes; G1 Phase; Humans; Immunohistochemistry; Ki-67 Antigen; Liver Neoplasms; Lymph Nodes; Polyethylene Glycols; Sensitivity and Specificity; Serum Albumin, Bovine; Stomach Neoplasms; Tongue Neoplasms; Tyramine | 2005 |
Affinity chromatographic selection of carbonylated proteins followed by identification of oxidation sites using tandem mass spectrometry.
Topics: Animals; Biotin; Chromatography, Affinity; Fungal Proteins; Liver; Metals; Nitroparaffins; Oxidation-Reduction; Oxidative Stress; Propane; Protein Carbonylation; Proteins; Rats; Sensitivity and Specificity; Serum Albumin, Bovine; Tandem Mass Spectrometry | 2005 |
Double-wavelength technique for surface plasmon resonance measurements: basic concept and applications for single sensors and two-dimensional sensor arrays.
Topics: Biosensing Techniques; Biotin; DNA; Hepacivirus; In Situ Hybridization; Oligonucleotides; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Surface Plasmon Resonance; Temperature | 2005 |
Oligonucleotide array for simultaneous detection of respiratory viruses using a reverse-line blot hybridization assay.
Topics: Biotin; DNA, Complementary; DNA, Viral; Humans; Luminescent Measurements; Molecular Diagnostic Techniques; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Respiratory Tract Infections; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Sensitivity and Specificity; Staining and Labeling; Virus Diseases; Viruses | 2005 |
Application of a liposomal bioluminescent label in the development of a flow injection immunoanalytical system.
Topics: Aequorin; Antibodies; Biotin; Calibration; Flow Injection Analysis; Immunoassay; Liposomes; Luminescent Measurements; Sensitivity and Specificity; Time Factors | 2005 |
Magnetic tweezers tease out dissociation.
Topics: Biotin; Ligands; Magnetics; Microscopy, Atomic Force; Sensitivity and Specificity; Serum Albumin, Bovine; Streptavidin; Surface Properties; Viscosity | 2005 |
Sensitive non-isotopic assays for autoantibodies to IA-2 and to a combination of both IA-2 and GAD65.
Topics: Antibody Specificity; Autoantibodies; Autoantigens; Biotin; Diabetes Mellitus; Enzyme-Linked Immunosorbent Assay; Glutamate Decarboxylase; Humans; Iodine Radioisotopes; Isoenzymes; Membrane Proteins; Protein Tyrosine Phosphatase, Non-Receptor Type 1; Protein Tyrosine Phosphatases; Receptor-Like Protein Tyrosine Phosphatases, Class 8; Reproducibility of Results; Sensitivity and Specificity | 2005 |
Amperometric determination of live Escherichia coli using antibody-coated paramagnetic beads.
Topics: Aminophenols; Antibodies; beta-Galactosidase; Biosensing Techniques; Biotin; Calibration; Electrochemistry; Electrodes; Escherichia coli; Immunoassay; Magnetics; Microspheres; Sensitivity and Specificity | 2005 |
Development of a time-resolved immunofluorometric assay for quantifying platelet-derived microparticles in human plasma.
Topics: Antibodies, Monoclonal; beta-Thromboglobulin; Biomarkers; Biotin; Biotinylation; Blood Platelets; Calcimycin; Detergents; Europium; Flow Cytometry; Fluoroimmunoassay; Humans; Ionophores; Isotopes; Particle Size; Peptide Fragments; Platelet Activation; Platelet Membrane Glycoprotein IIb; Sensitivity and Specificity; Streptavidin | 2006 |
Detection of single bacterial pathogens with semiconductor quantum dots.
Topics: Biotin; Color; Escherichia coli O157; Microscopy, Fluorescence; Quantum Dots; Semiconductors; Sensitivity and Specificity; Spectrometry, Fluorescence | 2005 |
Self-referencing a single waveguide grating sensor in a micron-sized deep flow chamber for label-free biomolecular binding assays.
Topics: Biotin; Image Enhancement; Microchip Analytical Procedures; Microfluidics; Optics and Photonics; Protein Binding; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Time Factors | 2005 |
Polydiacetylene (PDA)-based colorimetric detection of biotin-streptavidin interactions.
Topics: Acetylene; Biosensing Techniques; Biotin; Colorimetry; Liposomes; Polyacetylene Polymer; Polymers; Polyynes; Protein Array Analysis; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2006 |
Novel detection system for biomolecules using nano-sized bacterial magnetic particles and magnetic force microscopy.
Topics: Bacterial Proteins; Biomarkers; Biosensing Techniques; Biotin; Biotinylation; Feasibility Studies; Magnetics; Magnetospirillum; Microscopy, Atomic Force; Models, Biological; Nanostructures; Nanotechnology; Particle Size; Protein Binding; Sensitivity and Specificity; Streptavidin | 2005 |
Flow cytometer in the infrared: inexpensive modifications to a commercial instrument.
Topics: Biotin; Costs and Cost Analysis; Flow Cytometry; Fluorescent Dyes; HL-60 Cells; Humans; Immunophenotyping; Infrared Rays; Lasers; Leukocyte Common Antigens; Leukocytes; Microspheres; Sensitivity and Specificity; Streptavidin | 2005 |
Sample patterning on NMR surface microcoils.
Topics: Avidin; Biotin; Equipment Design; Liposomes; Magnetic Resonance Spectroscopy; Sensitivity and Specificity | 2006 |
Quantum dots as efficient energy acceptors in a time-resolved fluoroimmunoassay.
Topics: Biotin; Cadmium Compounds; Chelating Agents; Energy Transfer; Fluoroimmunoassay; Luminescent Measurements; Molecular Structure; Quantum Dots; Selenium Compounds; Sensitivity and Specificity; Streptavidin; Sulfides; Terbium; Time Factors; Zinc Compounds | 2005 |
Molecular recognition of avidin on biotin-functionalized gold surfaces detected by FT-IRRAS and use of metal carbonyl probes.
Topics: Avidin; Biotin; Enzymes, Immobilized; Gold; Molecular Structure; Organometallic Compounds; Protein Binding; Sensitivity and Specificity; Spectroscopy, Fourier Transform Infrared; Surface Properties | 2002 |
A strategy for sensitivity and specificity enhancements in prostate specific antigen-alpha1-antichymotrypsin detection based on surface plasmon resonance.
Topics: alpha 1-Antichymotrypsin; Biotin; Ethylene Glycol; Humans; Immunoassay; Prostate-Specific Antigen; Sensitivity and Specificity; Surface Plasmon Resonance | 2006 |
Engineering camel single-domain antibodies and immobilization chemistry for human prostate-specific antigen sensing.
Topics: Animals; Antibodies; Biosensing Techniques; Biotin; Camelus; Histidine; Humans; Lysine; Male; Nickel; Prostate-Specific Antigen; Protein Engineering; Sensitivity and Specificity; Streptavidin | 2005 |
SIRT1 top 40 hits: use of one-bead, one-compound acetyl-peptide libraries and quantum dots to probe deacetylase specificity.
Topics: Acetylation; Amino Acids; Biotin; Catalysis; Histone Deacetylases; Humans; Lysine; Mass Spectrometry; NAD; Peptide Library; Quantum Dots; Reproducibility of Results; Sensitivity and Specificity; Sirtuin 1; Sirtuins; Spectrometry, Fluorescence; Streptavidin; Substrate Specificity | 2006 |
A facile method for coding and labeling assays on polystyrene beads with differently colored luminescent nanocrystals.
Topics: Biotin; Cadmium Compounds; Lasers; Luminescence; Luminescent Measurements; Microscopy, Confocal; Nanoparticles; Polystyrenes; Quantum Dots; Selenium Compounds; Sensitivity and Specificity; Streptavidin; Sulfides; Surface Properties; Zinc Compounds | 2006 |
Correlating the fractional inhibition of caspase-3 in NT2 cells with apoptotic markers using an active-caspase-3 enzyme-linked immunosorbent assay.
Topics: Apoptosis; Biotin; Caspase 3; Caspase Inhibitors; Caspases; Cell Line; Coumarins; Cysteine Proteinase Inhibitors; Dipeptides; DNA Fragmentation; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Humans; Inhibitory Concentration 50; Oligopeptides; Poly(ADP-ribose) Polymerase Inhibitors; Sensitivity and Specificity; Spectrin | 2006 |
Scanning electrochemical microscopy. 56. Probing outside and inside single giant liposomes containing Ru(bpy)32+.
Topics: 2,2'-Dipyridyl; Biotin; Coordination Complexes; Electrochemistry; Glass; Liposomes; Microscopy, Scanning Probe; Particle Size; Polylysine; Sensitivity and Specificity; Surface Properties | 2006 |
MOSFET-Embedded microcantilevers for measuring deflection in biomolecular sensors.
Topics: Binding Sites; Biosensing Techniques; Biotin; Finite Element Analysis; Immunoglobulin G; Nanotechnology; Protein Binding; Sensitivity and Specificity; Streptavidin; Transistors, Electronic | 2006 |
Functional peptide microarrays for specific and sensitive antibody diagnostics.
Topics: Amino Acid Sequence; Antibodies, Monoclonal; Antigens, Viral; Biotin; Epitopes; Humans; Immunoassay; Molecular Diagnostic Techniques; Molecular Structure; Peptides; Protein Array Analysis; Sensitivity and Specificity | 2006 |
Biomolecular interaction analysis under electrophoretic flow conditions.
Topics: Antibodies; Biotin; DNA; Electrophoresis; Microscopy, Interference; Oligonucleotides; Oligonucleotides, Antisense; Polymorphism, Single Nucleotide; Sensitivity and Specificity; Streptavidin; Time Factors | 2006 |
A new synthetic protocol for labeled oligonucleotides, using a chemically cleavable universal linker.
Topics: Biotin; Chromatography, High Pressure Liquid; Homeodomain Proteins; Molecular Structure; Oligonucleotides; Organophosphorus Compounds; Sensitivity and Specificity; Structure-Activity Relationship; Time Factors; Transcription Factors; Uridine | 2006 |
Nonregeneration protocol for surface plasmon resonance: study of high-affinity interaction with high-density biosensors.
Topics: Animals; Antibodies; Biosensing Techniques; Biotin; Immunoglobulin G; Kinetics; Ligands; Models, Chemical; Molecular Structure; Rabbits; Sensitivity and Specificity; Streptavidin; Surface Plasmon Resonance; Time Factors | 2006 |
High-sensitivity bacterial detection using biotin-tagged phage and quantum-dot nanocomplexes.
Topics: Bacteriophages; Biotin; Flow Cytometry; Gram-Negative Bacteria; Gram-Positive Bacteria; Microscopy, Fluorescence; Nanotechnology; Quantum Dots; Sensitivity and Specificity; Time Factors | 2006 |
Analysis of individual data from bead-based assays ("bead arrays").
Topics: Biotin; Fluorescence; Fluorometry; Microspheres; Regression Analysis; Sensitivity and Specificity; Streptavidin | 2006 |
Grafting of poly(ethylene glycol) onto poly(acrylic acid)-coated glass for a protein-resistant surface.
Topics: Acrylic Resins; Adsorption; Biocompatible Materials; Biosensing Techniques; Biotin; Carbodiimides; Enzymes, Immobilized; Glass; Microscopy, Atomic Force; Myosin Subfragments; Polyethylene Glycols; Propanols; Sensitivity and Specificity; Silanes; Surface Properties | 2006 |
Digital readout of target binding with attomole detection limits via enzyme amplification in femtoliter arrays.
Topics: beta-Galactosidase; Biotin; Immunoenzyme Techniques; Microarray Analysis; Nanotechnology; Sensitivity and Specificity; Streptavidin | 2006 |
Xenon biosensor amplification via dendrimer-cage supramolecular constructs.
Topics: Biosensing Techniques; Biotin; Contrast Media; Dendrimers; Magnetic Resonance Imaging; Sensitivity and Specificity; Xenon | 2006 |
A multiplex DNA suspension microarray for simultaneous detection and differentiation of classical swine fever virus and other pestiviruses.
Topics: 5' Untranslated Regions; Biotin; Fluorescence; Genotype; Microspheres; Oligonucleotide Array Sequence Analysis; Pestivirus; Polymerase Chain Reaction; RNA, Viral; Sensitivity and Specificity; Staining and Labeling; Streptavidin | 2006 |
Correlation between serological immune response analyzed by a new ELISA for HPV-16/18 E7 oncoprotein and clinical characteristics of cervical cancer patients.
Topics: Antibodies, Viral; Antibody Specificity; Biomarkers; Biotin; Cancer Vaccines; Carcinoma; Disease Progression; DNA-Binding Proteins; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunoglobulin G; Oncogene Proteins, Viral; Papillomavirus E7 Proteins; Papillomavirus Vaccines; Precancerous Conditions; Recombinant Proteins; Sensitivity and Specificity; Streptavidin; Uterine Cervical Neoplasms; Vaccination | 2006 |
A microsphere immunoassay for detection of antibodies to avian influenza virus.
Topics: Animals; Antibodies, Viral; Biotin; Chickens; Enzyme-Linked Immunosorbent Assay; Fluorescence; Immunoassay; Influenza A virus; Influenza in Birds; Microspheres; Nucleocapsid Proteins; Nucleoproteins; Recombinant Proteins; RNA-Binding Proteins; Sensitivity and Specificity; Statistics as Topic; Streptavidin; Viral Core Proteins | 2006 |
Immunomagnetic beads assay for the detection of botulinum neurotoxin types C and D.
Topics: Antibodies, Monoclonal; Binding, Competitive; Biotin; Botulinum Toxins; Clostridium botulinum; Immunoassay; Immunomagnetic Separation; Sensitivity and Specificity | 2006 |
Fluorescence quenching of water-soluble conjugated polymer by metal cations and its application in sensor.
Topics: Avidin; Biotin; Cations; Fluorescent Dyes; Ligands; Metals, Heavy; Polyvinyls; Sensitivity and Specificity; Spectrometry, Fluorescence; Streptavidin | 2007 |
Estimation of dielectric function of biotin-capped gold nanoparticles via signal enhancement on surface plasmon resonance.
Topics: Adsorption; Biotin; Gold; Metal Nanoparticles; Microscopy, Atomic Force; Particle Size; Sensitivity and Specificity; Surface Plasmon Resonance; Surface Properties | 2006 |
Development of a biotin-streptavidin amplified enzyme immunoassay for oxytocin and its application during milk ejection and the reproductive cycle in the mithun (Bos frontalis).
Topics: Animals; Area Under Curve; Bacterial Proteins; Biotin; Cattle; Estrus; Female; Immunoenzyme Techniques; Lactation; Milk; Oxytocin; Sensitivity and Specificity; Time Factors | 2006 |
Surface plasmon resonance-based highly sensitive immunosensing for brain natriuretic peptide using nanobeads for signal amplification.
Topics: Antibodies, Monoclonal; Biotin; Immunoassay; Models, Molecular; Nanoparticles; Natriuretic Peptide, Brain; Sensitivity and Specificity; Streptavidin; Surface Plasmon Resonance | 2006 |
Microwave-triggered metal-enhanced chemiluminescence (MT-MEC): application to ultra-fast and ultra-sensitive clinical assays.
Topics: Avidin; Biotin; Humans; Indicators and Reagents; Luminescent Measurements; Metals; Microwaves; Proteins; Sensitivity and Specificity; Silver | 2006 |
Ultra-sensitive colorimetric method to quantitate hundreds of polynucleotide molecules by gold nanoparticles with silver enhancement.
Topics: Avidin; Base Sequence; Biotin; Chemistry Techniques, Analytical; Colorimetry; Gold; Metal Nanoparticles; Molecular Sequence Data; Nanostructures; Nucleic Acid Hybridization; Oligonucleotides; Polynucleotides; Sensitivity and Specificity; Silver; Surface Properties | 2006 |
Esterase 2-oligodeoxynucleotide conjugates as sensitive reporter for electrochemical detection of nucleic acid hybridization.
Topics: Base Pair Mismatch; Biotin; DNA; Electrochemistry; Esterases; Nucleic Acid Hybridization; Oligodeoxyribonucleotides; Sensitivity and Specificity | 2007 |
Molecular imaging using a targeted magnetic resonance hyperpolarized biosensor.
Topics: Avidin; Biosensing Techniques; Biotin; Contrast Media; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Microspheres; Sensitivity and Specificity; Sepharose; Time Factors; Xenon Isotopes | 2006 |
Cyclic voltammetric responses of horseradish peroxidase multilayers on electrodes.
Topics: Avidin; Biosensing Techniques; Biotin; Catalysis; Horseradish Peroxidase; Hydrogen Peroxide; Models, Theoretical; Oxidation-Reduction; Sensitivity and Specificity; Surface Properties | 2006 |
Sensitive protein detection via triple-binder proximity ligation assays.
Topics: Antibodies; Biotin; Humans; Immunoassay; Molecular Probe Techniques; Nucleic Acid Hybridization; Oligonucleotides; Polymerase Chain Reaction; Prostate-Specific Antigen; Proteins; Sensitivity and Specificity; Streptavidin; Troponin I; U937 Cells; Vascular Endothelial Growth Factor A | 2007 |
Comparison of two polymer-based immunohistochemical detection systems: ENVISION+ and ImmPRESS.
Topics: Animals; Antibodies, Monoclonal; Antigen-Antibody Reactions; Antigens; Aspergillus; Biotin; Caliciviridae; Cats; Dogs; Formaldehyde; Immunohistochemistry; Mice; Paraffin Embedding; Polymers; Rabbits; Reagent Kits, Diagnostic; Sensitivity and Specificity; Staining and Labeling; Tissue Fixation; West Nile virus | 2006 |
Development of zeptomole and attomolar detection sensitivity of biotin-peptide using a dot-blot gold nanoparticle immunoassay.
Topics: Antibodies; Biotin; Gold; Immunoblotting; Metal Nanoparticles; Nanotechnology; Peptides; Sensitivity and Specificity | 2007 |
Development of Listeria monocytogenes-specific immunomagnetic beads using a single-chain antibody fragment.
Topics: Antibodies, Bacterial; Antibody Specificity; Biotin; Food Contamination; Food Microbiology; Immunoglobulin Variable Region; Immunomagnetic Separation; Listeria monocytogenes; Plasmids; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2007 |
Silica colloidal crystals for enhanced fluorescence detection in microarrays.
Topics: Biotin; Colloids; Crystallization; Fluorescein; Fluorescence; Microarray Analysis; Sensitivity and Specificity; Silicon Dioxide; Streptavidin | 2007 |
Steric crowding effects on target detection in an affinity biosensor.
Topics: Animals; Antigen-Antibody Reactions; Binding Sites; Biosensing Techniques; Biotin; Goats; Immunoglobulin G; Kinetics; Rabbits; Sensitivity and Specificity; Silicon; Streptavidin | 2007 |
Ultrasensitive densitometry detection of cytokines with nanoparticle-modified aptamers.
Topics: Aptamers, Nucleotide; Becaplermin; Biotin; Cytokines; Densitometry; Enzyme-Linked Immunosorbent Assay; Gold; Metal Nanoparticles; Platelet-Derived Growth Factor; Proto-Oncogene Proteins c-sis; Reproducibility of Results; SELEX Aptamer Technique; Sensitivity and Specificity; Silver | 2007 |
Enhanced electrochemical detection of DNA hybridization with carbon nanotube modified paste electrode.
Topics: Adsorption; Base Pair Mismatch; Biotin; DNA; DNA, Complementary; Electrochemistry; Electrodes; Gold; Nanoparticles; Nanostructures; Nanotechnology; Nanotubes, Carbon; Nucleic Acid Hybridization; Oligonucleotides; Particle Size; Porosity; Sensitivity and Specificity; Silicon Dioxide; Solutions; Streptavidin; Surface Properties | 2007 |
Photonic crystal surface waves for optical biosensors.
Topics: Biosensing Techniques; Biotin; Crystallization; Optics and Photonics; Photons; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Time Factors | 2007 |
Label-free detection of bacteria by electrochemical impedance spectroscopy: comparison to surface plasmon resonance.
Topics: Antibodies, Bacterial; Avidin; Bacteria; Biosensing Techniques; Biotin; Electric Impedance; Electrodes; Equipment Design; Escherichia coli O157; Gold; Immunoassay; Immunoblotting; Sensitivity and Specificity; Spectrum Analysis; Surface Plasmon Resonance | 2007 |
Plasmonic detection of a model analyte in serum by a gold nanorod sensor.
Topics: Biosensing Techniques; Biotin; Glass; Gold; Humans; Metal Nanoparticles; Nanotubes; Sensitivity and Specificity; Serum; Streptavidin; Surface Plasmon Resonance | 2007 |
A semi-automated and highly sensitive streptavidin magnetic capture-hybridization RT-PCR assay: application to genus-wide or species-specific detection of several viruses of ornamental bulb crops.
Topics: Biotin; Plant Diseases; Plant Viruses; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; Streptavidin | 2007 |
Application of the avidin-biotin interaction to immobilize DNA in the development of electrochemical impedance genosensors.
Topics: Avidin; Biocompatible Materials; Biosensing Techniques; Biotin; DNA; Electric Impedance; Electrochemistry; Electrodes; Electron Transport; Epoxy Resins; Ferricyanides; Ferrocyanides; Graphite; Oligonucleotides; Oxidation-Reduction; Sensitivity and Specificity | 2007 |
Surface plasmon resonance spectroscopy study of interfacial binding of thrombin to antithrombin DNA aptamers.
Topics: Antithrombins; Aptamers, Nucleotide; Base Sequence; Binding Sites; Biosensing Techniques; Biotin; DNA; Enzyme-Linked Immunosorbent Assay; Fibrinogen; Hydrocarbons; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Surface Plasmon Resonance; Surface Properties; Thrombin; Time Factors | 2007 |
Evaluation of indirect and avidin-biotin enzyme linked immunosorbent assays for detection of anti-listeriolysin O antibodies in bovine milk samples.
Topics: Animals; Antibodies, Bacterial; Avidin; Biotin; Cattle; Dairying; Enzyme-Linked Immunosorbent Assay; Female; Food Microbiology; Humans; India; Listeria monocytogenes; Milk; Predictive Value of Tests; ROC Curve; Sensitivity and Specificity | 2007 |
Rapid genotyping of CYP2D6, CYP2C19 and TPMT polymorphisms by primer extension reaction in a dipstick format.
Topics: 5' Untranslated Regions; Aryl Hydrocarbon Hydroxylases; Base Sequence; Biotin; Cytochrome P-450 CYP2C19; Cytochrome P-450 CYP2D6; Deoxyuracil Nucleotides; DNA Mutational Analysis; DNA Primers; Genome; Genotype; Methyltransferases; Mixed Function Oxygenases; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Sensitivity and Specificity; Sequence Analysis, DNA; Streptavidin | 2007 |
Using of HPLC coupled with coulometric detector for the determination of biotin in pharmaceuticals.
Topics: Biotin; Chromatography, High Pressure Liquid; Electrochemistry; Molecular Structure; Pharmaceutical Preparations; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet; Technology, Pharmaceutical | 2007 |
Biotinylated thermoresponsive micelle self-assembled from double-hydrophilic block copolymer for drug delivery and tumor target.
Topics: Acrylic Resins; Biotin; Biotinylation; Cell Line; Cell Survival; Drug Carriers; Humans; Magnetic Resonance Spectroscopy; Micelles; Microscopy, Confocal; Microscopy, Electron, Transmission; Molecular Structure; Neoplasms; Polyethylene Glycols; Sensitivity and Specificity; Temperature; Water | 2008 |
A novel magnetic bead-based assay with high sensitivity and selectivity for analysis of telomerase in exfoliated cells from patients with bladder and colon cancer.
Topics: Bacterial Proteins; Biomarkers, Tumor; Biosensing Techniques; Biotin; Colonic Neoplasms; Feces; Horseradish Peroxidase; Humans; Immunomagnetic Separation; Luminescent Measurements; Neoplasm Proteins; Oligonucleotide Probes; Reference Standards; Sensitivity and Specificity; Staining and Labeling; Telomerase; Uracil Nucleotides; Urinary Bladder Neoplasms; Urine | 2007 |
Luminescent energy transfer between cadmium telluride nanoparticle and lanthanide(III) chelate in competitive bioaffinity assays of biotin and estradiol.
Topics: Biotin; Cadmium Compounds; Chelating Agents; Estradiol; Fluorescence Resonance Energy Transfer; Lanthanoid Series Elements; Luminescence; Metal Nanoparticles; Sensitivity and Specificity; Tellurium | 2007 |
Electrochemical fabrication and evaluation of highly sensitive nanorod-modified electrodes for a biotin/avidin system.
Topics: Avidin; Biosensing Techniques; Biotin; Electrochemistry; Electroplating; Equipment Design; Equipment Failure Analysis; Microelectrodes; Nanotubes; Reproducibility of Results; Sensitivity and Specificity | 2008 |
Preparation, characterization, and application of biotinylated and biotin-PEGylated glucagon-like peptide-1 analogues for enhanced oral delivery.
Topics: Absorption; Administration, Oral; Animals; Binding Sites; Biotin; Biotinylation; Blood Glucose; Diabetes Mellitus, Type 2; Enzyme Stability; Glucagon-Like Peptide 1; Hypoglycemic Agents; Injections, Intraperitoneal; Intestinal Mucosa; Lysine; Mice; Peptide Hydrolases; Polyethylene Glycols; Rats; Sensitivity and Specificity | 2008 |
A simplified, sensitive immunohistochemical detection system employing signal amplification based on fluorescyl-tyramide/antifluorescein antibody reaction: its application to pathologic testing and research.
Topics: Animals; Antibodies; Antigens, Neoplasm; Biotin; Humans; Immunoenzyme Techniques; Immunohistochemistry; Mice; Mice, SCID; Microscopy, Fluorescence; Neoplasms; Paraffin Embedding; Sensitivity and Specificity; Tyramine | 2008 |
Electric field-enhanced sensitivity of grafted ligands and receptors.
Topics: Biotin; Colloids; Electromagnetic Fields; Glass; Latex Fixation Tests; Ligands; Particle Size; Reagent Kits, Diagnostic; Rheumatoid Factor; Ribonuclease, Pancreatic; Sensitivity and Specificity; Streptavidin; Tin Compounds | 2008 |
[Method of antibody analysis based on visual protein array].
Topics: Antibodies; Antigens; Biosensing Techniques; Biotin; Electrochemistry; Fluorescent Dyes; Hypersensitivity; Immunoassay; Immunoglobulin E; Immunoglobulin G; Immunoglobulins; Limit of Detection; Metal Nanoparticles; Protein Array Analysis; Proteomics; Sensitivity and Specificity | 2008 |
Label-free plasmonic detection of biomolecular binding by a single gold nanorod.
Topics: Biosensing Techniques; Biotin; Gold; Metal Nanoparticles; Microscopy, Atomic Force; Nanotubes; Particle Size; Sensitivity and Specificity; Streptavidin; Surface Plasmon Resonance; Surface Properties; Time Factors | 2008 |
A high-complexity, multiplexed solution-phase assay for profiling protease activity on microarrays.
Topics: Amino Acid Sequence; Base Sequence; Biological Assay; Biotin; DNA; Endopeptidases; Enzyme-Linked Immunosorbent Assay; Histidine; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Peptides; Reproducibility of Results; Sensitivity and Specificity; Solutions; Substrate Specificity | 2008 |
Rapid identification of reactive cysteine residues for site-specific labeling of antibody-Fabs.
Topics: Antibodies, Monoclonal; Antigen-Antibody Reactions; Binding Sites; Biotin; Cell Line, Tumor; Chromatography, Liquid; Cysteine; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Gene Expression Regulation; Genetic Engineering; Genetic Variation; Humans; Immunoglobulin Fab Fragments; Maleimides; Mass Spectrometry; Models, Molecular; Mutagenesis, Site-Directed; Plasmids; Sensitivity and Specificity; Staining and Labeling; Surface Properties | 2008 |
Liposome-based immunostrip for the rapid detection of Salmonella.
Topics: Aged; Antibodies, Bacterial; Antigens, Bacterial; Benzenesulfonates; Biotin; Child; Collodion; Cross Reactions; Escherichia coli O157; Humans; Immunoassay; Liposomes; Listeria; Microscopy, Atomic Force; Salmonella Infections; Salmonella typhimurium; Sensitivity and Specificity; Staining and Labeling | 2008 |
A novel high-throughput format assay for HIV-1 integrase strand transfer reaction using magnetic beads.
Topics: Biological Assay; Biotin; Digoxin; DNA; Drug Evaluation, Preclinical; Enzyme Inhibitors; HIV Integrase Inhibitors; HIV-1; Humans; Inhibitory Concentration 50; Magnetics; Microspheres; Sensitivity and Specificity; Streptavidin; Time Factors; Vitamin B Complex | 2008 |
Detection in near-field domain of biomolecules adsorbed on a single metallic nanoparticle.
Topics: Biosensing Techniques; Biotin; Gold; Metal Nanoparticles; Microscopy, Electron, Scanning; Nanotechnology; Protein Binding; Sensitivity and Specificity; Silver; Streptavidin; Surface Plasmon Resonance | 2008 |
Enzyme-linked amperometric electrochemical genosensor assay for the detection of PCR amplicons on a streptavidin-treated screen-printed carbon electrode.
Topics: Antibodies; Bacterial Outer Membrane Proteins; Biosensing Techniques; Biotin; Carbon; DNA, Bacterial; Electrochemistry; Electrons; Fluorescein; Horseradish Peroxidase; Hydrogen Peroxide; Polymerase Chain Reaction; Sensitivity and Specificity; Streptavidin; Vibrio cholerae | 2008 |
Signal amplification via intramolecular energy transfer using tripodal neutral iridium(III) complexes upon binding to avidin.
Topics: Avidin; Binding Sites; Biotin; Energy Transfer; Hydrophobic and Hydrophilic Interactions; Iridium; Luminescence; Molecular Structure; Organometallic Compounds; Sensitivity and Specificity | 2008 |
Colloidal-based localized surface plasmon resonance (LSPR) biosensor for the quantitative determination of stanozolol.
Topics: Antibodies; Antigens; Biosensing Techniques; Biotin; Gold Colloid; Nanoparticles; Reproducibility of Results; Sensitivity and Specificity; Silanes; Stanozolol; Streptavidin; Surface Plasmon Resonance; Surface Properties | 2008 |
Scintigraphic imaging of vertebral osteomyelitis with 111in-biotin.
Topics: Adolescent; Adult; Aged; Aged, 80 and over; Biotin; Chi-Square Distribution; Female; Humans; Image Interpretation, Computer-Assisted; Male; Middle Aged; Osteomyelitis; Pentetic Acid; Radionuclide Imaging; Sensitivity and Specificity; Spinal Diseases | 2008 |
Quartz crystal microbalance analysis of growth kinetics for aggregation intermediates of the amyloid-beta protein.
Topics: Amyloid beta-Peptides; Biotin; Crystallization; Hydrogen-Ion Concentration; Kinetics; Models, Biological; Osmolar Concentration; Peptide Fragments; Protein Binding; Quartz; Sensitivity and Specificity | 2008 |
A sensitive three-step protocol for fluorescence-based Western blot detection.
Topics: Antibodies; Biotin; Blotting, Western; Cell Line; Fluorescence; Fluorescent Dyes; Fluoroimmunoassay; Humans; Keratin-8; Keratinocytes; NF-kappa B; p38 Mitogen-Activated Protein Kinases; Sensitivity and Specificity; Streptavidin | 2008 |
Biotinylation and analysis of membrane-bound and soluble proteins.
Topics: Avidin; Biotin; Membrane Proteins; Sensitivity and Specificity; Solubility; Streptavidin | 2001 |
Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance.
Topics: Aptamers, Nucleotide; Avidin; Biosensing Techniques; Biotin; Dendrimers; Humans; Reproducibility of Results; Sensitivity and Specificity; Serum Albumin; Streptavidin; Sulfhydryl Compounds; Surface Plasmon Resonance; Thrombin | 2008 |
Description of the entire mRNA population by a 3' end cDNA fragment generated by class IIS restriction enzymes.
Topics: Animals; Base Sequence; Biotin; Cloning, Molecular; Deoxyribonucleases, Type II Site-Specific; DNA, Complementary; Gene Expression; Kidney; Liver; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Polydeoxyribonucleotides; Polymerase Chain Reaction; RNA, Messenger; Sensitivity and Specificity; Sequence Analysis, DNA | 1995 |
Evaluation of non-radioactive labelling and detection of deoxyribonucleic acids. Part One: Chemiluminescent methods.
Topics: Adamantane; Base Sequence; Biotin; Blotting, Southern; Deoxyuracil Nucleotides; Digoxigenin; DNA; Humans; Indicators and Reagents; Luminescent Measurements; Molecular Sequence Data; Peroxidases; Sensitivity and Specificity | 1995 |
Poly A-linked colorimetric microtiter plate assay for HIV reverse transcriptase.
Topics: Biotin; Colorimetry; Deoxyuracil Nucleotides; Digoxigenin; Enzyme-Linked Immunosorbent Assay; HIV Reverse Transcriptase; HIV-1; Humans; Indicators and Reagents; Leukocytes, Mononuclear; Poly A; Retroviridae; RNA-Directed DNA Polymerase; Sensitivity and Specificity | 1993 |
An integrated target sequence and signal amplification assay, reverse transcriptase-PCR-enzyme-linked immunosorbent assay, to detect and characterize flaviviruses.
Topics: Aedes; Animals; Base Sequence; Biotin; Culex; Dengue; Dengue Virus; Digoxigenin; DNA Probes; DNA, Viral; Encephalitis Virus, St. Louis; Enzyme-Linked Immunosorbent Assay; Flavivirus; Gene Amplification; Humans; Molecular Sequence Data; Polymerase Chain Reaction; RNA-Directed DNA Polymerase; RNA, Viral; Sensitivity and Specificity; Viremia | 1994 |
Multiple anterograde tracing, combining Phaseolus vulgaris leucoagglutinin with rhodamine- and biotin-conjugated dextran amine.
Topics: Animals; Biological Transport; Biotin; Blood Vessels; Cerebrovascular Circulation; Cross Reactions; Dextrans; Female; Fluorescent Dyes; Immunohistochemistry; Limbic System; Methods; Neural Pathways; Phytohemagglutinins; Rats; Rats, Wistar; Rhodamines; Sensitivity and Specificity; Synaptic Transmission; Thalamic Nuclei | 1994 |
Expression of galactoside-specific endogenous lectins and their ligands in human oral squamous cell carcinoma.
Topics: Acetylgalactosamine; Biopsy; Biotin; Carcinoma, Squamous Cell; Cell Differentiation; Galactosides; Glycoproteins; Humans; Lectins; Ligands; Mistletoe; Mouth Neoplasms; Plant Lectins; Plants, Medicinal; Sensitivity and Specificity; Staining and Labeling | 1994 |
Nonradioactive labeling and high-sensitive detection of PCR products.
Topics: Biotechnology; Biotin; Blotting, Southern; Digoxigenin; DNA; DNA-Directed DNA Polymerase; Electrophoresis, Agar Gel; Gene Amplification; Immunoassay; Molecular Probes; Nucleic Acid Hybridization; Polymerase Chain Reaction; RNA; Sensitivity and Specificity; Taq Polymerase | 1994 |
Sensitive immunocytochemical detection of viral genomes.
Topics: Biotin; DNA Probes; DNA, Viral; Frozen Sections; Genome, Viral; Humans; Immunohistochemistry; In Situ Hybridization; Indicators and Reagents; Methenamine; Molecular Probe Techniques; Sensitivity and Specificity; Staining and Labeling | 1994 |
The effects of varying key steps in the non-radioactive in situ hybridization protocol: a quantitative study.
Topics: Animals; Biotin; Endopeptidase K; Female; In Situ Hybridization; Oligonucleotide Probes; Pancreas; Proinsulin; Rats; Rats, Wistar; Reproducibility of Results; RNA, Messenger; Sensitivity and Specificity; Serine Endopeptidases; Staining and Labeling; Temperature; Time Factors; Tissue Fixation | 1995 |
Comparable sensitivities for detection of HIV-1 reverse transcriptase (RT) and other polymerases by RT assays requiring no radioisotopic materials.
Topics: Animals; Avian Leukosis Virus; Avian Myeloblastosis Virus; Biological Assay; Biotin; Cell Line; DNA-Directed DNA Polymerase; Escherichia coli; HIV Reverse Transcriptase; Humans; Radioisotopes; RNA-Directed DNA Polymerase; Sensitivity and Specificity | 1995 |
Nonradioactive assay for sequence-specific DNA binding proteins.
Topics: Animals; Antibodies, Anti-Idiotypic; Base Sequence; Biotin; Cell Extracts; Cell Line; DNA-Binding Proteins; Enzyme-Linked Immunosorbent Assay; Molecular Sequence Data; Oligonucleotide Probes; Sensitivity and Specificity; Tumor Suppressor Protein p53 | 1995 |
A comparison of digoxigenin and biotin labelled DNA and RNA probes for in situ hybridization.
Topics: Animals; Biotin; Brain; Chickens; Digoxigenin; DNA, Viral; Humans; In Situ Hybridization; Measles virus; Molecular Probe Techniques; RNA, Viral; Sensitivity and Specificity | 1995 |
Streptavidin-reactive protein in Fasciola hepatica.
Topics: Animals; Antibodies, Helminth; Antigens, Helminth; Bacterial Proteins; Biotin; Carrier Proteins; Enzyme-Linked Immunosorbent Assay; False Positive Reactions; Fasciola hepatica; Helminth Proteins; Immunoglobulin E; Male; Mice; Mice, Inbred BALB C; Molecular Weight; Protein Binding; Sensitivity and Specificity; Streptavidin | 1995 |
Double determinant immuno-polymerase chain reaction: a sensitive method for detecting circulating antigens in human sera.
Topics: Antibodies, Monoclonal; Base Sequence; Biotin; DNA Probes; Enzyme-Linked Immunosorbent Assay; Gene Amplification; Humans; Intercellular Adhesion Molecule-1; Molecular Sequence Data; Pancreatic Neoplasms; Polymerase Chain Reaction; Sensitivity and Specificity; Solubility; Stomach Neoplasms; Tumor Cells, Cultured | 1995 |
Identification of malaria-infected dried mosquitoes by biotinylated DNA probe.
Topics: Animals; Base Sequence; Biotin; Culicidae; Desiccation; DNA Probes; DNA, Protozoan; Isotope Labeling; Molecular Sequence Data; Plasmodium falciparum; Repetitive Sequences, Nucleic Acid; Sensitivity and Specificity | 1995 |
Development of a two-site immuno-PCR assay for hepatitis B surface antigen.
Topics: Antibodies, Monoclonal; Bacterial Proteins; Base Sequence; Biotin; Blotting, Southern; Bluetongue virus; DNA, Viral; Hepatitis B Antibodies; Hepatitis B Surface Antigens; Humans; Immunoassay; Molecular Sequence Data; Polymerase Chain Reaction; Sensitivity and Specificity; Streptavidin | 1995 |
Identification of blood meals of Phlebotomus perniciosus (Diptera: Psychodidae) in Spain by a competitive enzyme-linked immunosorbent assay biotin/avidin method.
Topics: Animals; Avidin; Biotin; Blood; Enzyme-Linked Immunosorbent Assay; Food Preferences; Host-Parasite Interactions; Humans; Phlebotomus; Sensitivity and Specificity; Spain | 1995 |
PCR-SSCP: nonradioisotopic detection with biotinylated primers and streptavidin-alkaline phosphatase conjugate.
Topics: Alkaline Phosphatase; Bacterial Proteins; Base Sequence; Biotin; Cytomegalovirus; DNA Primers; DNA, Viral; Humans; Leukocytes; Molecular Sequence Data; Mutation; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Sensitivity and Specificity; Streptavidin; Viral Envelope Proteins | 1995 |
Triple-label hybridization assay for type-1 diabetes-related HLA alleles.
Topics: Alleles; Base Sequence; Biotin; Diabetes Mellitus, Type 1; DNA; DNA Probes; Europium; Fluorometry; Genotype; HLA-DQ Antigens; HLA-DQ beta-Chains; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; Polymorphism, Genetic; Samarium; Sensitivity and Specificity; Terbium | 1995 |
Ultra-sensitive FISH using peroxidase-mediated deposition of biotin- or fluorochrome tyramides.
Topics: Animals; Biotin; Coumarins; Fluorescein; Fluoresceins; In Situ Hybridization, Fluorescence; Mice; Peroxidases; Rhodamines; Sensitivity and Specificity; Tyramine | 1995 |
Homogeneous immunoassay of whole-blood samples.
Topics: Antibodies, Monoclonal; Biotin; Blood Coagulation; Factor Xa; Ferric Compounds; Humans; Immunoassay; Indicators and Reagents; Kinetics; Microspheres; Photolysis; Sensitivity and Specificity; Serine Proteinase Inhibitors; Thrombin | 1995 |
Ligand western blotting for specific detection of active forms of proteases.
Topics: Bacterial Proteins; Biotin; Blotting, Western; Collodion; Electrophoresis, Polyacrylamide Gel; Fibrinolysin; Humans; Hydrogen-Ion Concentration; Luminescent Measurements; Microchemistry; Pancreatic Juice; Sensitivity and Specificity; Serine Endopeptidases; Streptavidin; Trypsin; Trypsin Inhibitors | 1995 |
Chemiluminescent enzyme-linked immunoassay for reverse transcriptase, illustrated by detection of HIV reverse transcriptase.
Topics: Alkaline Phosphatase; Antibodies; Bacterial Proteins; Biotin; Deoxyuracil Nucleotides; Digoxigenin; Enzyme-Linked Immunosorbent Assay; HIV Infections; HIV Reverse Transcriptase; Humans; Luminescent Measurements; Lymphocytes; Oligodeoxyribonucleotides; Poly A; Reference Standards; RNA-Directed DNA Polymerase; Sensitivity and Specificity; Streptavidin; Time Factors | 1993 |
A rapid and specific method for the detection of PCR products.
Topics: Biotin; Colorimetry; DNA; Ethidium; Immunoenzyme Techniques; Nucleic Acid Hybridization; Polymerase Chain Reaction; RNA; Sensitivity and Specificity | 1993 |
A microtiter assay for factor XIII using fibrinogen and biotinylcadaverine as substrates.
Topics: Biotin; Cadaverine; Factor XIII; Fibrinogen; Humans; Sensitivity and Specificity | 1994 |
Determination of biotin concentration by a competitive enzyme-linked immunosorbent assay (ELISA) method.
Topics: Animals; Bacteria; Bacterial Proteins; Biotin; Enzyme-Linked Immunosorbent Assay; Horseradish Peroxidase; Rabbits; Sensitivity and Specificity; Streptavidin | 1994 |
Evaluation of SHARP signal system for enzymatic detection of amplified hepatitis B virus DNA.
Topics: Base Sequence; Biotin; DNA Primers; DNA Probes; DNA, Viral; Evaluation Studies as Topic; Gene Amplification; Hepatitis B; Hepatitis B virus; Humans; Immunoenzyme Techniques; Molecular Sequence Data; Polymerase Chain Reaction; Sensitivity and Specificity; Viremia; Virology | 1995 |
Improvement of an EIA system for basic fibroblast growth factor by use of biotinylated antibody prepared with NHS-LC-biotin.
Topics: Adult; Antibodies; Biotin; Fibroblast Growth Factor 2; Humans; Immunoenzyme Techniques; Isoelectric Focusing; Male; Reproducibility of Results; Sensitivity and Specificity; Succinimides | 1995 |
Enzyme immunoassay of immunoreactive progastrin-releasing peptide(31-98) as tumor marker for small-cell lung carcinoma: development and evaluation.
Topics: Amino Acid Sequence; Antibodies, Monoclonal; Base Sequence; Biomarkers, Tumor; Biotin; Carcinoma, Small Cell; Enzyme-Linked Immunosorbent Assay; Humans; Lung Neoplasms; Microchemistry; Molecular Sequence Data; Peptide Fragments; Peptides; Recombinant Proteins; Reference Values; Sensitivity and Specificity | 1995 |
6 alpha-biotinylated estrone: novel tracer in competitive chemiluminescence immunoassay of estrone in serum.
Topics: Bacterial Proteins; Binding, Competitive; Biotin; Estrone; Female; Humans; Immunoassay; Luminescent Measurements; Male; Polycystic Ovary Syndrome; Reference Values; Sensitivity and Specificity; Streptavidin | 1995 |
A non-isotopic immunoassay for guanosine 3':5'-cyclic monophosphate using a cyclic GMP-biotin conjugate as tracer.
Topics: Biotin; Chromatography, Liquid; Cyclic GMP; Immunoassay; Reproducibility of Results; Sensitivity and Specificity | 1995 |
Detection and typing of human papillomaviruses by in situ hybridization with biotinylated oligonucleotide mixtures.
Topics: Biotin; Cervix Uteri; Condylomata Acuminata; DNA Probes, HPV; Female; Humans; In Situ Hybridization; Papillomaviridae; Papillomavirus Infections; Sensitivity and Specificity; Tumor Virus Infections; Uterine Cervical Dysplasia; Uterine Cervical Neoplasms; Vulvar Neoplasms | 1995 |
Effect of beta-mercaptoethanol on the detection of biotinylated proteins.
Topics: Biotin; Cell Line; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Fibrosarcoma; Humans; Indicators and Reagents; Iodine Radioisotopes; Luminescent Measurements; Mercaptoethanol; Neoplasm Proteins; Proteins; Sensitivity and Specificity; Serum Albumin, Bovine; Tumor Cells, Cultured | 1995 |
A comparison of three avidin-biotin complex immunoenzyme systems for detection of African swine fever virus antigen in paraffin-embedded tissues.
Topics: African Swine Fever Virus; Animals; Antigens, Viral; Avidin; Biotin; Fluorescent Antibody Technique; Immunoenzyme Techniques; Lymph Nodes; Microscopy, Fluorescence; Sensitivity and Specificity; Spleen; Swine | 1995 |
Determination of human tumour necrosis factor-alpha (TNF-alpha) by time-resolved immunofluorometric assay.
Topics: Antibodies, Monoclonal; Bacterial Proteins; Biotin; Chromatography, Gel; Chromatography, Liquid; Enzyme-Linked Immunosorbent Assay; Female; Fluoroimmunoassay; Humans; Male; Sensitivity and Specificity; Streptavidin; Tumor Necrosis Factor-alpha | 1994 |
Chemiluminescent detection of amplified pseudorabies virus gp50 DNA with immobilized probes on microtiter wells.
Topics: Base Sequence; Biotin; Colorimetry; DNA Primers; DNA Probes; DNA, Viral; Herpesvirus 1, Suid; Luminescent Measurements; Molecular Sequence Data; Polymerase Chain Reaction; Sensitivity and Specificity; Viral Envelope Proteins | 1994 |
The biotin/avidin-mediated microtiter plate lectin assay with the use of chemically modified glycoprotein ligand.
Topics: Acetylgalactosamine; Animals; Binding, Competitive; Biotin; Carbohydrate Conformation; Carbohydrate Sequence; Cattle; Enzyme-Linked Immunosorbent Assay; Galactose; Glycophorins; Glycoproteins; Humans; Lectins; Ligands; Molecular Sequence Data; Mucins; Sensitivity and Specificity; Sheep | 1994 |
A novel peptide probe for studying the transbilayer movement of phosphatidylethanolamine.
Topics: Animals; Anti-Bacterial Agents; Biotin; Cattle; Erythrocyte Membrane; Humans; Lipid Bilayers; Peptides; Peptides, Cyclic; Phosphatidylethanolamines; Protein Binding; Rabbits; Rats; Sensitivity and Specificity; Sheep; Streptomycetaceae; Temperature; Translocation, Genetic | 1994 |
The SA/rABC technique: a new ABC procedure for detection of antigens at increased sensitivity.
Topics: Animals; Antibodies; Avidin; Biotin; Cerebellar Cortex; Enzyme-Linked Immunosorbent Assay; gamma-Aminobutyric Acid; Immunoenzyme Techniques; Rats; Sensitivity and Specificity | 1995 |
Co-expression of collagen types II and X mRNAs in newly formed hypertrophic chondrocytes of the embryonic chick vertebral body demonstrated by double-fluorescence in situ hybridization.
Topics: Animals; Biotin; Cartilage; Chick Embryo; Collagen; Digoxigenin; DNA Probes; DNA, Complementary; Gene Expression Regulation, Developmental; In Situ Hybridization, Fluorescence; Osteogenesis; RNA, Messenger; Sensitivity and Specificity; Thoracic Vertebrae; Thymine Nucleotides; Tritium | 1994 |
Agar plate method using Lactobacillus plantarum for biotin determination in serum and urine.
Topics: Agar; Biological Assay; Biotin; Lactobacillus; Sensitivity and Specificity | 1994 |
A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine.
Topics: Biotin; Cells, Cultured; Chromosome Mapping; DNA Probes; Humans; In Situ Hybridization; Mitosis; Sensitivity and Specificity; Tyramine | 1995 |
Streptavidin-biotin based time-resolved immunofluorometric assay for direct measurement of high concentrations of human chorionic gonadotropin (hCG).
Topics: Bacterial Proteins; Biotin; Chorionic Gonadotropin; Female; Fluoroimmunoassay; Humans; Pregnancy; Sensitivity and Specificity; Streptavidin | 1994 |
Detection and quantitation of hepatitis C virus RNA in serum using the polymerase chain reaction and a colorimetric enzymatic detection system.
Topics: Animals; Avidin; Base Sequence; Biotin; Colorimetry; DNA, Complementary; Electrophoresis, Agar Gel; Hepacivirus; Hepatitis C; Molecular Sequence Data; Pan troglodytes; Peroxidase; Polymerase Chain Reaction; RNA, Viral; Sensitivity and Specificity; Viremia | 1994 |
Pre-targeted immunodetection in glioma patients: tumour localization and single-photon emission tomography imaging of [99mTc]PnAO-biotin.
Topics: Astrocytoma; Biotin; Brain Neoplasms; Glioblastoma; Humans; Magnetic Resonance Imaging; Organotechnetium Compounds; Radioimmunodetection; Sensitivity and Specificity; Tissue Distribution; Tomography, Emission-Computed, Single-Photon; Tomography, X-Ray Computed | 1994 |
Detection of enteroviruses in cerebrospinal fluids: enzymatic amplification and hybridization with a biotinylated riboprobe.
Topics: Base Sequence; Biotin; Cells, Cultured; Child; DNA, Viral; Enterovirus; Enterovirus Infections; Fibroblasts; Gene Amplification; Genome, Viral; Humans; Lung; Meningitis, Viral; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; RNA Probes; Sensitivity and Specificity | 1994 |
Peroxidase labelling immunocytochemistry: a comparison of eleven commercially-available avidin-biotin systems.
Topics: Avidin; Biotin; Costs and Cost Analysis; Immunoenzyme Techniques; Nonprescription Drugs; Sensitivity and Specificity | 1993 |
Determination of chondroitin-6-sulphate by a competitive enzyme immunoassay using a biotinylated antigen.
Topics: Antibodies, Monoclonal; Antigens; Binding, Competitive; Biotin; Cartilage; Cells, Cultured; Chondroitin Sulfates; Chromatography, High Pressure Liquid; Cross Reactions; Culture Media; Female; Humans; Immunoenzyme Techniques; Kinetics; Male; Reference Values; Sensitivity and Specificity | 1994 |
Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation.
Topics: Base Sequence; Biotin; Blotting, Northern; Cell Adhesion Molecules; Cells, Cultured; Deoxyuracil Nucleotides; DNA; Evaluation Studies as Topic; Gene Expression; Humans; Intercellular Adhesion Molecule-1; Molecular Sequence Data; Muscle, Smooth, Vascular; Polymerase Chain Reaction; Reproducibility of Results; RNA, Messenger; Sensitivity and Specificity | 1993 |
Enzyme-linked immunosorbent assay and enzyme-linked coagulation assay for detection of Clostridium botulinum neurotoxins A, B, and E and solution-phase complexes with dual-label antibodies.
Topics: Antibodies, Bacterial; Avidin; Biotin; Blood Coagulation Tests; Botulinum Toxins; Clostridium botulinum; Enzyme-Linked Immunosorbent Assay; Immunoglobulin G; Neurotoxins; Sensitivity and Specificity; Species Specificity | 1994 |
Use of fluorescent substrate 4-MUP in the detection of biotin-labeled DNA probes.
Topics: Biotin; DNA Probes; Fluorescence; Hymecromone; Nucleic Acid Hybridization; Sensitivity and Specificity | 1993 |
Avidin-biotin system in radioimmunoguided surgery for colorectal cancer. Advantages and limits.
Topics: Antibodies, Monoclonal; Avidin; Biotin; Colorectal Neoplasms; False Negative Reactions; False Positive Reactions; Humans; Injections; Intraoperative Care; Iodine Radioisotopes; Neoplasm Recurrence, Local; Predictive Value of Tests; Preoperative Care; Radioimmunodetection; Sensitivity and Specificity; Surgical Procedures, Operative; Time Factors; Treatment Outcome | 1994 |
A polymerase chain reaction-sequence-specific oligonucleotide procedure for HLA class II typing using biotin- and digoxigenin-labeled probes simultaneously in hybridization.
Topics: Base Sequence; Biotin; Cell Line; Digoxigenin; DNA; Genotype; HLA-DR Antigens; Humans; Immunoblotting; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity | 1994 |
A sensitive ELISA for human tissue-type plasminogen activator applicable to the study of acute release from cultured human endothelial cells.
Topics: Avidin; Biotin; Cells, Cultured; Culture Media; Endothelium, Vascular; Enzyme-Linked Immunosorbent Assay; Humans; Plasminogen Activator Inhibitor 1; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Subcellular Fractions; Thrombin; Tissue Plasminogen Activator | 1994 |
Biotinylated and cysteine-modified peptides as useful reagents for studying the inhibition of cathepsin G.
Topics: alpha 1-Antichymotrypsin; Amino Acid Sequence; Biotin; Cathepsin G; Cathepsins; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Peptides; Reproducibility of Results; Sensitivity and Specificity; Serine Endopeptidases; Tritium | 1994 |
Detection of quantitative polymerase chain reaction products by hybridization on magnetic support with 125I-radiolabeled probes: quantification of c-myc copy numbers.
Topics: Bacterial Proteins; Base Sequence; Biotin; DNA Probes; Genes, myc; Humans; Iodine Radioisotopes; Magnetics; Molecular Sequence Data; Neoplasms; Nucleic Acid Hybridization; Polymerase Chain Reaction; Reference Standards; Sensitivity and Specificity; Streptavidin; Tumor Cells, Cultured | 1993 |
Quantitative measurement of nonisotopically labeled polymerase chain reaction product.
Topics: Base Sequence; Biotin; Digoxigenin; DNA; DNA Probes; Isotope Labeling; Molecular Sequence Data; Polymerase Chain Reaction; RNA Probes; Sensitivity and Specificity | 1993 |
Multiple primer pairs for polymerase chain reaction (PCR) amplification of human parvovirus B19 DNA.
Topics: Acute Disease; Biotin; Chronic Disease; Disease Outbreaks; DNA Primers; DNA, Viral; Erythema Infectiosum; Genome, Viral; Humans; Parvovirus B19, Human; Polymerase Chain Reaction; Sensitivity and Specificity; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid | 1993 |
Sensitive and quantitative detection of PCR-amplified HIV-1 DNA products by an enzyme linked immunoassay following solution hybridization with two differently labelled oligonucleotide probes.
Topics: Base Sequence; Biotin; Cell Line; Digoxigenin; DNA Primers; DNA, Viral; Enzyme-Linked Immunosorbent Assay; Genes, gag; HIV-1; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity | 1993 |
Chemiluminescent detection of strand displacement amplified DNA from species comprising the Mycobacterium tuberculosis complex.
Topics: Base Sequence; Biotin; DNA Polymerase I; DNA Transposable Elements; DNA, Bacterial; False Positive Reactions; Gene Amplification; Humans; Luminescent Measurements; Molecular Sequence Data; Mycobacterium; Mycobacterium bovis; Mycobacterium tuberculosis; Nucleic Acid Hybridization; Oligonucleotide Probes; Sensitivity and Specificity; Tuberculosis | 1993 |
Fine-structural localization of proenkephalin mRNAs in the hypothalamic magnocellular dorsal nucleus of the guinea pig: a comparison of radioisotopic and enzymatic in situ hybridization methods at the light- and electron-microscopic levels.
Topics: Alkaline Phosphatase; Animals; Biotin; Cytoplasm; Digoxigenin; Endoplasmic Reticulum; Enkephalins; Guinea Pigs; Hypothalamus; In Situ Hybridization; Male; Microscopy, Electron; Nuclear Envelope; Oligonucleotide Probes; Protein Precursors; RNA, Messenger; Sensitivity and Specificity; Sulfur Radioisotopes | 1993 |
Dot immunoassay with biotinylated antigen for determination of antibodies against the circulating cathodic antigen (CCA) in schistosomiasis japonica.
Topics: Animals; Antibodies, Helminth; Antibodies, Monoclonal; Antigens, Helminth; Biotin; Humans; Immunoblotting; Schistosoma japonicum; Schistosomiasis japonica; Sensitivity and Specificity | 1993 |
An assay method for herpes simplex virus type 1 seroprevalence survey--detection of antibody in saliva by avidin-biotin complex enzyme-linked immunosorbent assay (ABC-ELISA).
Topics: Adolescent; Adult; Antibodies, Viral; Avidin; Biotin; Enzyme-Linked Immunosorbent Assay; Female; Herpes Simplex; Herpesvirus 1, Human; Humans; Male; Saliva; Sensitivity and Specificity; Seroepidemiologic Studies | 1993 |
A rate turbidimetric immunoassay for theophylline using biotin-avidin system.
Topics: Avidin; Biotin; Evaluation Studies as Topic; Fluorescence Polarization Immunoassay; Humans; Immunoassay; Nephelometry and Turbidimetry; Sensitivity and Specificity; Theophylline | 1993 |
Avidin-biotin based electrochemical immunoassay for thyrotropin.
Topics: Alkaline Phosphatase; Antibodies, Monoclonal; Avidin; Biotin; Electrochemistry; Humans; Immunoenzyme Techniques; Sensitivity and Specificity; Spectrophotometry, Ultraviolet; Thyrotropin | 1993 |
Comparison of 3' and 5' biotin labelled oligonucleotides for in situ hybridisation.
Topics: Animals; Base Sequence; Biotin; Cells, Cultured; Haplorhini; HeLa Cells; Humans; In Situ Hybridization; Molecular Sequence Data; Oligonucleotide Probes; Paraffin Embedding; Picornaviridae Infections; Poly T; Rhinovirus; RNA, Viral; Sensitivity and Specificity | 1993 |
Localization of EGF receptors in frozen tissue sections by antibody and biotinylated EGF-based techniques.
Topics: Animals; Antibodies, Monoclonal; Biotin; Epidermal Growth Factor; ErbB Receptors; Female; Frozen Sections; Humans; Immunoenzyme Techniques; Ligands; Liver; Male; Mice; Microtomy; Neoplasms; Rats; Sensitivity and Specificity; Skin | 1994 |
A solid-phase protein assay: quantitation of protein in the nanogram range.
Topics: Animals; Avidin; Biotin; Cattle; Cell Extracts; Chemistry Techniques, Analytical; Colorimetry; Enzyme-Linked Immunosorbent Assay; Fibrinogen; Horseradish Peroxidase; Humans; Keratinocytes; Microchemistry; Ovalbumin; Proteins; Sensitivity and Specificity; Serum Albumin, Bovine; Succinimides | 1993 |
Biotin labelling of chicken antibodies and their subsequent use in ELISA and immunohistochemistry.
Topics: Animals; Biotin; Chickens; Enzyme-Linked Immunosorbent Assay; Humans; Immunoenzyme Techniques; Immunoglobulin G; Immunoglobulin M; Mammals; Mice; Sensitivity and Specificity; Species Specificity | 1993 |
Detection of Staphylococcus aureus with biotinylated monoclonal antibodies directed against staphylococcal TNase complexed to avidin-peroxidase in a rapid sandwich enzyme-linked immunofiltration assay (sELIFA).
Topics: Antibodies, Monoclonal; Avidin; Bacteremia; Biotin; Enzyme-Linked Immunosorbent Assay; Humans; Immunoenzyme Techniques; Micrococcal Nuclease; Sensitivity and Specificity; Staphylococcal Infections; Staphylococcus aureus | 1993 |
A europium fluoroimmunoassay for measuring binding of antigen to class II MHC glycoproteins.
Topics: Amino Acid Sequence; Antigen-Antibody Reactions; Antigen-Presenting Cells; Bacterial Proteins; Biotin; Dose-Response Relationship, Immunologic; Europium; Fluoroimmunoassay; Histocompatibility Antigens Class II; Humans; Hydrogen-Ion Concentration; Insulin; Molecular Sequence Data; Peptides; Sensitivity and Specificity; Streptavidin; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha | 1993 |
Brugia malayi and Brugia pahangi: synthetic biotin labeling of oligonucleotide probes for use in species-specific detection assays.
Topics: Animals; Base Sequence; Biotin; Brugia malayi; Brugia pahangi; DNA; Microfilariae; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Sensitivity and Specificity; Species Specificity | 1993 |
Protein A antibody-capture ELISA (PACE): an ELISA format to avoid denaturation of surface-adsorbed antigens.
Topics: Amino Acid Sequence; Antibodies, Monoclonal; Antibody Specificity; Antigen-Antibody Complex; Antigens; Apoproteins; Binding, Competitive; Biotin; Cytochrome c Group; Cytochromes c; Enzyme-Linked Immunosorbent Assay; Molecular Sequence Data; Protein Conformation; Radioimmunoassay; Saccharomyces cerevisiae Proteins; Sensitivity and Specificity; Staphylococcal Protein A | 1993 |
Synthesis and biological properties of a biotinylated derivative of ACTH1-17 for MSH receptor studies.
Topics: Adrenocorticotropic Hormone; Amino Acid Sequence; Animals; Biological Assay; Biotin; Humans; Melanins; Melanoma, Experimental; Mice; Molecular Sequence Data; Peptide Fragments; Receptors, Pituitary Hormone; Sensitivity and Specificity; Tumor Cells, Cultured | 1993 |
Development of an amplified enzyme-linked immunosorbent assay for sensitive measurement of apolipoprotein B in plasma and tissue culture media.
Topics: Apolipoproteins B; Avidin; Biotin; Culture Media; Enzyme-Linked Immunosorbent Assay; Humans; Nephelometry and Turbidimetry; Reference Standards; Sensitivity and Specificity | 1993 |
Selective biotinylation of Neisseria meningitidis group B capsular polysaccharide and application in an improved ELISA for the detection of specific antibodies.
Topics: Animals; Antibodies, Bacterial; Bacterial Capsules; Biotin; Enzyme-Linked Immunosorbent Assay; Immunoenzyme Techniques; Immunoglobulin G; Immunoglobulin M; Mice; Mice, Inbred BALB C; Neisseria meningitidis; Polysaccharides, Bacterial; Sensitivity and Specificity | 1993 |
Enzyme complex amplification--a signal amplification method for use in enzyme immunoassays.
Topics: Bacterial Proteins; Biotin; Enzyme-Linked Immunosorbent Assay; Horseradish Peroxidase; Immunoblotting; Immunoenzyme Techniques; Methods; Sensitivity and Specificity; Streptavidin | 1993 |
A highly sensitive and fast nonradioactive method for detection of polymerase chain reaction products.
Topics: Adamantane; Bacterial Proteins; Biotin; Deoxyuracil Nucleotides; Digoxigenin; DNA, Bacterial; Enzymes, Immobilized; Hymecromone; Indicators and Reagents; Listeria monocytogenes; Nitrophenols; Organophosphorus Compounds; Polymerase Chain Reaction; Sensitivity and Specificity; Streptavidin; Substrate Specificity; Thymine Nucleotides | 1993 |
Colorimetric detection for PCR amplified HIV-1 DNA using magnetic beads.
Topics: Bacterial Proteins; Base Sequence; Biotin; Colorimetry; Digoxigenin; DNA Probes; DNA, Single-Stranded; DNA, Viral; HIV-1; Humans; Magnetics; Microspheres; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Time Factors | 1993 |
In vivo labeling of Escherichia coli cell envelope proteins with N-hydroxysuccinimide esters of biotin.
Topics: Aminocaproates; Bacterial Outer Membrane Proteins; Biotin; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Esters; Sensitivity and Specificity; Succinimides | 1993 |
A sensitive peroxidase staining immunoblotting method for measuring total protein S in human plasma.
Topics: Avidin; Biotin; Blood Protein Electrophoresis; Blotting, Western; Carrier Proteins; Complement Inactivator Proteins; Electrophoresis, Polyacrylamide Gel; Glycoproteins; Humans; Immunoenzyme Techniques; Molecular Weight; Protein S; Sensitivity and Specificity | 1993 |
Detection of nucleic acids in the attomole range using polybiotinylated oligonucleotide probes.
Topics: Base Sequence; Biotin; Clostridium; DNA Probes; DNA, Bacterial; Molecular Sequence Data; Nucleic Acid Amplification Techniques; Nucleic Acid Hybridization; Oligonucleotides; Sensitivity and Specificity | 1995 |
Rapid and sensitive streptavidin-biotin amplified fluorogenic enzyme-linked immunosorbent-assay for direct detection and identification of dengue viral antigens in serum.
Topics: Animals; Antibodies, Viral; Antigens, Viral; Bacterial Proteins; Biotin; Cells, Cultured; Dengue Virus; Enzyme-Linked Immunosorbent Assay; Fluorescence; Humans; Immunoglobulin G; Mice; Sensitivity and Specificity; Streptavidin | 1995 |
Quantification and lowering of serum biotin.
Topics: Animals; Avidin; Bacterial Proteins; Biotin; Diet; Dogs; Enzyme-Linked Immunosorbent Assay; Injections, Intravenous; Rabbits; Reference Values; Sensitivity and Specificity; Streptavidin | 1995 |
Automated detection of digoxigenin-labelled B19 parvovirus amplicons by a capture hybridization assay.
Topics: Bacterial Proteins; Base Sequence; Biotin; Digoxigenin; DNA Probes; DNA, Viral; Erythema Infectiosum; Female; Humans; Immunoenzyme Techniques; Immunoglobulin Fab Fragments; Male; Molecular Sequence Data; Nucleic Acid Hybridization; Parvovirus B19, Human; Reagent Kits, Diagnostic; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 1995 |
Noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for gamma 2-melanocyte-stimulating hormone (gamma 2-MSH) and measurement of immunoreactive gamma 2-MSH in plasma of healthy subjects.
Topics: Adult; Amino Acid Sequence; Binding, Competitive; Biotin; Evaluation Studies as Topic; Humans; Immunochemistry; Immunoenzyme Techniques; Male; Melanocyte-Stimulating Hormones; Molecular Sequence Data; Molecular Weight; Reference Values; Sensitivity and Specificity | 1995 |
Multilayer peroxidase-labeled antibody method: comparison with labeled streptavidin-biotin method, avidin-biotin-peroxidase complex method, and peroxidase-antiperoxidase method.
Topics: Animals; Avidin; Bacterial Proteins; Biotin; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Immunoenzyme Techniques; Immunohistochemistry; Liver; Male; Peroxidases; Rabbits; Rats; Rats, Wistar; Sensitivity and Specificity; Streptavidin | 1995 |
An enzyme-linked immunosorbent assay using biotinylated heparan sulfate to evaluate the interactions of heparin-like molecules and basic fibroblast growth factor.
Topics: Binding, Competitive; Biotin; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Fibroblast Growth Factor 2; Heparin; Heparitin Sulfate; Protein Binding; Reproducibility of Results; Sensitivity and Specificity; Structure-Activity Relationship | 1995 |
DELISA: sensitive nonisotopic assay for GAD65 autoantibodies, a key risk-assessment marker for insulin-dependent diabetes mellitus.
Topics: Autoantibodies; Bacterial Proteins; Biotin; Diabetes Mellitus, Type 1; Enzyme-Linked Immunosorbent Assay; Glutamate Decarboxylase; Humans; Quality Control; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 1996 |
Simple and sensitive enzyme-linked immunosorbent assay for ivermectin.
Topics: Animals; Antibody Formation; Antiparasitic Agents; Avidin; Binding, Competitive; Biotin; Cross Reactions; Dose-Response Relationship, Immunologic; Enzyme-Linked Immunosorbent Assay; Gerbillinae; Immunoglobulin G; Ivermectin; Male; Peroxidase; Rabbits; Sensitivity and Specificity; Serum Albumin, Bovine | 1996 |
Development of scintillation proximity assays for E-selectin and their application in testing potential antagonists.
Topics: Antibodies, Monoclonal; Bacterial Proteins; Binding, Competitive; Biotin; Carcinoembryonic Antigen; E-Selectin; HL-60 Cells; Humans; Ligands; Microspheres; Oligosaccharides; Protein Binding; Reproducibility of Results; Sensitivity and Specificity; Sialyl Lewis X Antigen; Streptavidin | 1995 |
The measurement of progesterone in serum by a non-competitive idiometric assay.
Topics: Antibodies, Anti-Idiotypic; Binding, Competitive; Biotin; Europium; Fluoroimmunoassay; Humans; Hybridomas; Immunoglobulin G; Progesterone; Radioimmunoassay; Sensitivity and Specificity | 1995 |
Quantification of polymerase chain reaction products: enzyme immunoassay based systems for digoxigenin- and biotin-labelled products that quantify either total or specific amplicons.
Topics: Animals; Base Sequence; Biotin; Cattle; Deoxyuracil Nucleotides; Digoxigenin; DNA; Endothelium; Glyceraldehyde-3-Phosphate Dehydrogenases; Hydrogen-Ion Concentration; Immunoenzyme Techniques; Luminescent Measurements; Molecular Sequence Data; Peptidyl-Dipeptidase A; Polymerase Chain Reaction; Rats; Sensitivity and Specificity; Xanthine Oxidase | 1996 |
Biotin-avidin amplified enzyme-linked immunosorbent assay (ELISA) for the measurement of canine serum IgA, IgG and IgM.
Topics: Animals; Avidin; Biotin; Blood Specimen Collection; Dogs; Enzyme-Linked Immunosorbent Assay; Female; Immunodiffusion; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Male; Reproducibility of Results; Sensitivity and Specificity | 1996 |
[Determination of free d-biotin--comparison of instrumental and non-instrumental methods of analysis].
Topics: Agglutination Tests; Bacterial Proteins; Biomarkers; Biotin; Enzyme-Linked Immunosorbent Assay; Sensitivity and Specificity; Streptavidin | 1996 |
Enhancing immunoelectrochemiluminescence (IECL) for sensitive bacterial detection.
Topics: Antigens, Bacterial; Bacillus anthracis; Bacillus subtilis; Bacterial Proteins; Binding Sites, Antibody; Biotin; Electrochemistry; Escherichia coli; Flow Cytometry; Immunochemistry; Immunomagnetic Separation; Kinetics; Luminescent Measurements; Microscopy, Fluorescence; Sensitivity and Specificity; Streptavidin | 1996 |
Development of a sensitive peptide-based immunoassay: application to detection of the Jun and Fos oncoproteins.
Topics: Amino Acid Sequence; Biotin; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Glutathione Transferase; Leucine Zippers; Molecular Sequence Data; Oncogene Protein p65(gag-jun); Oncogene Proteins v-fos; Peptides; Protein Conformation; Recombinant Fusion Proteins; Sensitivity and Specificity | 1996 |
2',2'-Difluorodeoxycytidine (gemcitabine) induces apoptosis in myeloma cell lines resistant to steroids and 2-chlorodeoxyadenosine (2-CdA).
Topics: 2-Chloroadenosine; Antimetabolites, Antineoplastic; Apoptosis; Biotin; Deoxyadenosines; Deoxycytidine; Deoxyuracil Nucleotides; Dexamethasone; DNA Damage; Drug Resistance; Gemcitabine; Glucocorticoids; Hematopoietic Stem Cells; Humans; Multiple Myeloma; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Sensitivity and Specificity; Staining and Labeling; Steroids; Tumor Cells, Cultured | 1996 |
Purification of single-stranded M13 DNA by cooperative triple-helix-mediated affinity capture.
Topics: Bacteriophage M13; Base Sequence; Binding Sites; Biotin; Chromatography, High Pressure Liquid; DNA, Single-Stranded; DNA, Viral; Genetic Vectors; Indicators and Reagents; Molecular Sequence Data; Nucleic Acid Conformation; Oligodeoxyribonucleotides; Sensitivity and Specificity; Templates, Genetic | 1996 |
Immunocytochemical detection of regional protein changes in rat brain sections using computer-assisted image analysis.
Topics: Animals; Antigens; Biotin; Brain; Densitometry; Image Processing, Computer-Assisted; Immunohistochemistry; Male; Proteins; Rats; Rats, Sprague-Dawley; Receptors, GABA-A; Sensitivity and Specificity | 1996 |
A non-radioactive method for identifying enzyme-amplified products of the reticuloendotheliosis proviral env and LTR genes using psoralen-biotin labelled probes.
Topics: Animals; Biotin; Chick Embryo; Chickens; DNA Probes; DNA, Viral; Ducks; Ficusin; Gene Products, env; Polymerase Chain Reaction; Proviruses; Repetitive Sequences, Nucleic Acid; Reticuloendotheliosis virus; Sensitivity and Specificity; Turkey | 1996 |
Recombinant antigen-based avidin-biotin microtiter enzyme-linked immunosorbent assay for serodiagnosis of invasive amebiasis.
Topics: Animals; Antibodies, Protozoan; Antigens, Protozoan; Avidin; Biotin; Case-Control Studies; Cloning, Molecular; DNA, Complementary; Entamoeba histolytica; Entamoebiasis; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Humans; Molecular Sequence Data; Recombinant Fusion Proteins; Sensitivity and Specificity; Serologic Tests | 1996 |
Colorimetric solid-phase capture hybridization assay for detection of amplified Borrelia burgdorferi DNA.
Topics: Biotin; Borrelia burgdorferi Group; Colorimetry; DNA Probes; DNA, Bacterial; Humans; Lyme Disease; Nucleic Acid Hybridization; Polymerase Chain Reaction; Polystyrenes; Sensitivity and Specificity; Skin; Synovial Fluid; Templates, Genetic | 1996 |
Immunochromatographic analysis of bovine growth hormone releasing factor involving reversed-phase high-performance liquid chromatography-immunodetection.
Topics: Animals; Antibodies, Monoclonal; Bacterial Proteins; Biotin; Calibration; Cattle; Chromatography, Affinity; Chromatography, High Pressure Liquid; Enzyme-Linked Immunosorbent Assay; Fluorescein-5-isothiocyanate; Growth Hormone-Releasing Hormone; Immune Sera; Online Systems; Peptide Fragments; Plasma; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Streptavidin; Succinimides; Trypsin | 1996 |
Quantitative assay of PCR-amplified hepatitis B virus DNA using a peroxidase-labelled DNA probe and enhanced chemiluminescence.
Topics: Bacterial Proteins; Biotin; DNA Probes; DNA, Viral; Hepatitis B; Humans; Luminescent Measurements; Molecular Probe Techniques; Peroxidase; Polymerase Chain Reaction; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 1996 |
Characterization of biotinylated liposomes sensitive to temperature and pH: new tools for anti-cancer drug delivery.
Topics: Antineoplastic Agents; Biotin; Calorimetry, Differential Scanning; Chemical Phenomena; Chemistry, Pharmaceutical; Chemistry, Physical; Drug Delivery Systems; Hydrogen-Ion Concentration; Liposomes; Permeability; Sensitivity and Specificity; Temperature | 1996 |
Mass allele detection (MAD) of rare 5-HT1A structural variants with allele-specific amplification and electrochemiluminescent detection.
Topics: Alleles; Base Sequence; Biotin; DNA Primers; Electrophoresis, Polyacrylamide Gel; Ethnicity; Genetic Testing; Genetic Variation; Heterozygote; Humans; Luminescent Measurements; Molecular Sequence Data; Mutation; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Single-Stranded Conformational; Receptors, Serotonin; Receptors, Serotonin, 5-HT1; Sensitivity and Specificity | 1996 |
Sensitive and fast mutation detection by solid phase chemical cleavage.
Topics: Bacterial Proteins; Base Sequence; Biotin; DNA; DNA Probes; Female; Genetic Techniques; Humans; Molecular Sequence Data; Mutation; Osmium Tetroxide; Polymerase Chain Reaction; Pyruvate Dehydrogenase Complex Deficiency Disease; Sensitivity and Specificity; Sequence Deletion; Streptavidin | 1996 |
Non-specific serum binding to streptavidin in a biotinylated peptide based enzyme immunoassay.
Topics: Adult; Bacterial Proteins; Biotin; Enzyme-Linked Immunosorbent Assay; Humans; Peptides; Protein Binding; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Streptomyces | 1996 |
Bioluminescence hybridization assays using recombinant aequorin. Application to the detection of prostate-specific antigen mRNA.
Topics: Aequorin; Bacterial Proteins; Biotin; Calcium; DNA; DNA Primers; Humans; Luminescent Measurements; Male; Nucleic Acid Hybridization; Prostate-Specific Antigen; Recombinant Proteins; RNA, Messenger; Sensitivity and Specificity; Streptavidin; Tumor Cells, Cultured | 1996 |
A competitive chemiluminescent enzyme-linked immunosorbent assay for the determination of RMP-7 in human blood.
Topics: Alkaline Phosphatase; Amino Acid Sequence; Antibodies; Avidin; Biotin; Bradykinin; Enzyme-Linked Immunosorbent Assay; Hemocyanins; Humans; Luminescent Measurements; Reference Standards; Sensitivity and Specificity | 1996 |
Colorimetric ELISA measurement of specific mRNA on immobilized-oligonucleotide-coated microtiter plates by reverse transcription with biotinylated mononucleotides.
Topics: Animals; Binding, Competitive; Biotin; Blotting, Northern; Colorimetry; Enzyme-Linked Immunosorbent Assay; Globins; HL-60 Cells; Humans; Kinetics; Liver; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Rabbits; RNA-Directed DNA Polymerase; RNA, Messenger; Sensitivity and Specificity | 1996 |
Biotinylated hyaluronan: a versatile and highly sensitive probe capable of detecting nanogram levels of hyaluronan binding proteins (hyaladherins) on electroblots by a novel affinity detection procedure.
Topics: Aggrecans; Animals; Binding Sites; Biotin; Blotting, Western; Cattle; Chromatography, Agarose; Collodion; Electrochemistry; Extracellular Matrix Proteins; Fibroblasts; Humans; Hyaluronan Receptors; Hyaluronic Acid; Lectins, C-Type; Microchemistry; Molecular Probes; Proteins; Proteoglycans; Sensitivity and Specificity | 1996 |
Biotinylated trypsin and its application as a sensitive, versatile probe for the detection and characterisation of an ovine chondrocyte serine proteinase inhibitor using Western blotting.
Topics: Amino Acid Sequence; Animals; Biotin; Cartilage, Articular; Molecular Sequence Data; Molecular Weight; Osteoarthritis; Sensitivity and Specificity; Serine Proteinase Inhibitors; Sheep; Trypsin | 1996 |
[A quantitative of serum biotin with microplate-assay using affinity streptavidin].
Topics: Adult; Aged; Bacterial Proteins; Biological Assay; Biomarkers; Biotin; Female; Humans; Liver Diseases; Male; Middle Aged; Sensitivity and Specificity; Streptavidin | 1996 |
An enzyme-linked immunoassay for the 1,25-dihydroxyvitamin D3 receptor protein.
Topics: Animals; Antibody Specificity; Biotin; Blotting, Western; Cell Extracts; Enzyme-Linked Immunosorbent Assay; Immunoradiometric Assay; Linear Models; Receptors, Calcitriol; Reproducibility of Results; Sensitivity and Specificity; Swine; Tissue Extracts | 1996 |
[Diagnosis of urogenital chlamydiosis using dot hybridization with biotin-labeled DNA probes].
Topics: Biotin; Chlamydia Infections; Chlamydia trachomatis; DNA Probes; Female; Female Urogenital Diseases; Humans; Male; Male Urogenital Diseases; Nucleic Acid Hybridization; Plasmids; Polymerase Chain Reaction; Sensitivity and Specificity | 1996 |
Two-site immunofluorometric assay of intact salmon calcitonin with improved sensitivity.
Topics: Adult; Aged; Antibodies, Monoclonal; Biotin; Calcitonin; Europium; Fluoroimmunoassay; Humans; Male; Metals, Rare Earth; Middle Aged; Peptide Fragments; Sensitivity and Specificity | 1997 |
Nonradioactive method for quantitation of PCR products without hybridization with a specific probe.
Topics: Biotin; Deoxyadenine Nucleotides; Deoxyuracil Nucleotides; Humans; Monocytes; Polymerase Chain Reaction; Sensitivity and Specificity | 1997 |
A nonisotopic single-strand conformation polymorphism protocol using a direct blotting electrophoresis, a chemiluminescent detection system, and a multiplex approach.
Topics: Adamantane; Biotin; Blotting, Southern; Electrophoresis, Polyacrylamide Gel; Exons; Humans; Hypercholesterolemia; Luminescent Measurements; Mutation; Polymorphism, Single-Stranded Conformational; Receptors, LDL; Sensitivity and Specificity | 1994 |
Increased urinary excretion of 3-hydroxyisovaleric acid and decreased urinary excretion of biotin are sensitive early indicators of decreased biotin status in experimental biotin deficiency.
Topics: Adult; Analysis of Variance; Avidin; Biotin; Chromatography, High Pressure Liquid; Egg White; Female; Gas Chromatography-Mass Spectrometry; Humans; Male; Reference Values; Reproducibility of Results; Sensitivity and Specificity; Valerates | 1997 |
HLA class II typing in a microtitre plate format using digoxigenin-labelled amplified DNA and biotin-labelled oligonucleotide probes.
Topics: Base Sequence; Biotin; Digoxigenin; DNA; DNA Primers; Evaluation Studies as Topic; Histocompatibility Testing; HLA-D Antigens; Humans; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity | 1993 |
Immunomagnetic detection of Bacillus stearothermophilus spores in food and environmental samples.
Topics: Animals; Antibodies, Bacterial; Avidin; Biotin; Colony Count, Microbial; Environmental Microbiology; Enzyme-Linked Immunosorbent Assay; Food Microbiology; Geobacillus stearothermophilus; Immunoglobulin G; Immunomagnetic Separation; Milk; Sensitivity and Specificity; Soil Microbiology; Spores, Bacterial | 1997 |
Sensitive chemiluminescence in situ hybridization for the detection of human papillomavirus genomes in biopsy specimens.
Topics: Biotin; Digoxigenin; DNA, Viral; Fluorescein; Fluoresceins; Genome, Viral; HeLa Cells; Humans; In Situ Hybridization; Luminescent Measurements; Papillomaviridae; Papillomavirus Infections; Reproducibility of Results; Sensitivity and Specificity; Tumor Cells, Cultured; Tumor Virus Infections | 1997 |
Synthesis and characterization of highly sensitive heparin probes for detection of heparin-binding proteins.
Topics: Animals; Biotin; Carrier Proteins; Cells, Cultured; Fluoresceins; Heparin; Humans; Ligands; Membrane Proteins; Molecular Probes; Muscle, Smooth, Vascular; Protein Binding; Rats; Sensitivity and Specificity | 1997 |
Amplified enzyme-linked-immunofilter assays enable detection of 50-10(5) bacterial cells within 1 hour.
Topics: Antibodies, Bacterial; Avidin; Biotin; Chromogenic Compounds; Colony Count, Microbial; Escherichia coli; Immunoenzyme Techniques; Luminescent Measurements; Sensitivity and Specificity | 1997 |
Time-resolved fluoroimmunoassay for pituitary adenylate cyclase activating polypeptide 27 (PACAP27) using europium (III) ion chelate labeled streptavidin-biotin complex.
Topics: Animals; Bacterial Proteins; Biotin; Chelating Agents; Cross Reactions; Europium; Female; Fluoroimmunoassay; Linear Models; Male; Neuropeptides; Neurotransmitter Agents; Pituitary Adenylate Cyclase-Activating Polypeptide; Rats; Rats, Sprague-Dawley; Reference Standards; Sensitivity and Specificity; Serum Albumin, Bovine; Streptavidin; Time Factors | 1997 |
Determination of sugar specificity of jackfruit lectin by a simple sugar-lectin binding assay using microtiter plate.
Topics: Animals; Bacterial Proteins; Biochemistry; Biotin; Carbohydrate Metabolism; Carbohydrates; Fruit; Hemagglutination; Horseradish Peroxidase; Lectins; Molecular Sequence Data; Plant Lectins; Sensitivity and Specificity; Streptavidin; Substrate Specificity | 1997 |
Targeting of ultrasound contrast material. An in vitro feasibility study.
Topics: Animals; Avidin; Biotin; Contrast Media; Dogs; Feasibility Studies; In Vitro Techniques; Particle Size; Phantoms, Imaging; Sensitivity and Specificity; Sonication; Transducers; Ultrasonography | 1997 |
Immunomagnetic capture PCR to detect viable Cryptosporidium parvum oocysts from environmental samples.
Topics: Animals; Antibodies, Protozoan; Antigens, Protozoan; Biotin; Cryptosporidium parvum; DNA, Protozoan; Endonucleases; Environmental Microbiology; Feces; Female; Immunoglobulin G; Immunomagnetic Separation; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Rabbits; Sensitivity and Specificity | 1997 |
Use of synthetic antigens improves detection by enzyme-linked immunosorbent assay of antibodies against abortigenic Chlamydia psittaci in ruminants.
Topics: Animals; Antibodies, Bacterial; Antigens, Bacterial; Bacterial Outer Membrane Proteins; Bacterial Proteins; Biotin; Cattle; Chlamydophila psittaci; Complement Fixation Tests; Enzyme-Linked Immunosorbent Assay; Epitopes; Glycine; Lipopolysaccharides; Mice; Peptides; Psittacosis; Rats; Recombinant Proteins; Sensitivity and Specificity; Serine; Sheep; Specific Pathogen-Free Organisms; Streptavidin | 1997 |
Directional immobilization of heparin onto the nonporous surface of polystyrene microplates.
Topics: Alkaline Phosphatase; Aniline Compounds; Biotin; Chromatography, Affinity; Digoxigenin; Heparin; Heparin Lyase; Indicators and Reagents; Microchemistry; Organophosphorus Compounds; Polystyrenes; Sensitivity and Specificity; Sodium Bicarbonate | 1997 |
High-frequency ultrasonic detection of thrombi with a targeted contrast system.
Topics: Avidin; Biotin; Contrast Media; Emulsions; Fluorocarbons; Humans; Image Processing, Computer-Assisted; Models, Biological; Sensitivity and Specificity; Thrombosis; Ultrasonography, Interventional | 1997 |
Concentrations of biotin metabolites in human milk.
Topics: Adult; Biotin; Chromatography, High Pressure Liquid; Female; Humans; Infant Nutritional Physiological Phenomena; Infant, Newborn; Milk, Human; Nutritional Requirements; Postpartum Period; Reproducibility of Results; Sensitivity and Specificity; Time Factors | 1997 |
Tyramine amplification technique in routine immunohistochemistry.
Topics: Antibodies, Monoclonal; Biotin; Evaluation Studies as Topic; Immunohistochemistry; Sensitivity and Specificity; Tyrosine | 1997 |
Biotinyl-tyramide: a novel approach for electron microscopic immunocytochemistry.
Topics: Amylases; Animals; Biotin; Catalase; HSP70 Heat-Shock Proteins; Insulin; Liver; Microscopy, Immunoelectron; Pancreas; Rats; Sensitivity and Specificity; Staining and Labeling; Tyramine | 1997 |
Highly-sensitive and specific enzyme-linked immunosorbent assays for GAD65 autoantibodies using a thioredoxin-GAD65 fusion antigen.
Topics: Adolescent; Autoantibodies; Autoimmune Diseases; Biotin; Child; Child, Preschool; Diabetes Mellitus, Type 1; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Female; Glutamate Decarboxylase; Humans; Infant; Male; Radioimmunoassay; Recombinant Fusion Proteins; Sensitivity and Specificity; Thioredoxins | 1997 |
Development of an enzyme-linked immunosorbent assay for measurement of serum-associated ALX40-4C.
Topics: Animals; Anti-HIV Agents; Antiviral Agents; Avidin; Binding, Competitive; Biotin; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin G; Oligopeptides; Rabbits; Reproducibility of Results; Sensitivity and Specificity | 1997 |
Rapid direct diagnosis of mumps meningitis by ELISA capture technique.
Topics: Animals; Biotin; Child; Child, Preschool; Chlorocebus aethiops; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Guinea Pigs; Humans; Meningitis, Viral; Mumps; Prospective Studies; Rabbits; Reproducibility of Results; Retrospective Studies; Sensitivity and Specificity; Vero Cells | 1997 |
Interaction of immobilized avidin with an aequorin-biotin conjugate: an aequorin-linked assay for biotin.
Topics: Aequorin; Avidin; Binding Sites; Binding, Competitive; Biotin; Biotinylation; Luminescent Measurements; Magnetics; Models, Molecular; Protein Conformation; Recombinant Proteins; Sensitivity and Specificity | 1997 |
Detection of verotoxigenic Escherichia coli by magnetic capture-hybridization PCR.
Topics: Bacterial Toxins; Biotin; Colony Count, Microbial; DNA Probes; DNA, Bacterial; Escherichia coli; Food Microbiology; Genes, Bacterial; Magnetics; Nucleic Acid Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity; Shiga Toxin 1; Streptavidin | 1998 |
Direct detection of 8-oxodeoxyguanosine and 8-oxoguanine by avidin and its analogues.
Topics: 8-Hydroxy-2'-Deoxyguanosine; Aged; Alkalies; Animals; Avidin; Binding Sites; Biotin; Biotinylation; Cell Nucleus; Deoxyguanosine; DNA; DNA Damage; DNA-Binding Proteins; DNA, Single-Stranded; Dose-Response Relationship, Drug; Female; Fluorescein-5-isothiocyanate; Fluorescent Dyes; Guanine; Humans; Hydrogen Peroxide; Male; Methylene Blue; Motor Neuron Disease; Sensitivity and Specificity; Tumor Cells, Cultured | 1998 |
Fiber optic-based biosensor for ricin.
Topics: Adsorption; Avidin; Biosensing Techniques; Biotin; Fiber Optic Technology; Fluorometry; Fresh Water; Immunoassay; Lectins; Optical Fibers; Ricin; Sensitivity and Specificity | 1997 |
Biotin-labeled DNA probe in a PCR-based assay increases detection sensitivity for the equine hemoparasite Babesia caballi.
Topics: Animals; Babesia; Babesiosis; Biotin; Cattle; DNA Primers; DNA Probes; DNA, Protozoan; Genomic Library; Horse Diseases; Horses; Polymerase Chain Reaction; Sensitivity and Specificity | 1997 |
[HLA-DR typing using PCR-SSO with simultaneous hybridization of each dot blot membrane with a digoxigenin- and a biotin-marked probe].
Topics: Biotin; Digoxigenin; Histocompatibility Testing; HLA-DR Antigens; Humans; In Situ Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity | 1994 |
Detection of Babesia bovis using a DIG-labeled DNA probe.
Topics: Affinity Labels; Animals; Azides; Babesia bovis; Babesiosis; Biotin; Cattle; Cattle Diseases; Digoxigenin; DNA Probes; DNA, Protozoan; Electrophoresis, Agar Gel; Genetic Techniques; Nucleic Acid Hybridization; Sensitivity and Specificity | 1997 |
An ultrasensitive, nonisotopic immunoassay for hyaluronan using the streptavidin-biotin system.
Topics: Antibodies, Monoclonal; Binding, Competitive; Biotin; Cartilage; Chondroitin Sulfates; Fluoroimmunoassay; Humans; Hyaluronic Acid; Immunoassay; Keratan Sulfate; Liver Diseases; Proteoglycans; Radioimmunoassay; Sensitivity and Specificity; Streptavidin | 1998 |
Demonstration of Kaposi's sarcoma-associated herpes virus cyclin D homolog in cutaneous Kaposi's sarcoma by colorimetric in situ hybridization using a catalyzed signal amplification system.
Topics: 3,3'-Diaminobenzidine; Acquired Immunodeficiency Syndrome; Adult; Aged; Aged, 80 and over; Biomarkers; Biotin; Blotting, Southern; Colorimetry; Cyclin D; Cyclins; DNA, Viral; Female; Herpesvirus 8, Human; HIV Seronegativity; Humans; In Situ Hybridization; Lymphoma, AIDS-Related; Male; Middle Aged; Sarcoma, Kaposi; Sensitivity and Specificity; Skin Neoplasms; Viral Proteins | 1998 |
Direct observation of ligand colocalization on individual receptor molecules.
Topics: Binding Sites; Biotin; Dimyristoylphosphatidylcholine; Energy Transfer; Ligands; Microscopy, Fluorescence; Models, Biological; Models, Molecular; Receptors, Cell Surface; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 1998 |
An in situ hybridization histochemistry technique allowing simultaneous visualization by the use of confocal microscopy of three cellular mRNA species in individual neurons.
Topics: Adrenal Glands; Animals; Arginine Vasopressin; Biotin; Corticotropin-Releasing Hormone; Digoxigenin; Histological Techniques; In Situ Hybridization; Male; Microscopy, Confocal; Mixed Function Oxygenases; Multienzyme Complexes; Neurons; Paraventricular Hypothalamic Nucleus; Rats; Rats, Sprague-Dawley; RNA, Messenger; Sensitivity and Specificity; Sulfur Radioisotopes | 1998 |
Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification.
Topics: Biotin; Carcinoma, Transitional Cell; Chromosomes, Human, Pair 1; Chromosomes, Human, Pair 12; Digoxigenin; Fluorescent Dyes; Humans; In Situ Hybridization; Sensitivity and Specificity; Trinitrobenzenes; Tumor Cells, Cultured; Tyramine | 1998 |
A mixture of synthetic oligonucleotide probes labeled with biotin for the sensitive detection of potato spindle tuber viroid.
Topics: Base Sequence; Biotin; Digoxigenin; DNA Probes; DNA, Complementary; Molecular Sequence Data; Nucleic Acid Conformation; Nucleic Acid Hybridization; Oligonucleotide Probes; Plant Viruses; Sensitivity and Specificity; Solanum tuberosum; Viroids | 1998 |
Detection of low affinity interactions between peptides and heat shock proteins by chemiluminescence of enhanced avidity reactions (CLEAR).
Topics: Amino Acid Sequence; Animals; Biotechnology; Biotin; Dextrans; Evaluation Studies as Topic; Heat-Shock Proteins; Humans; In Vitro Techniques; Kinetics; Ligands; Luminescent Measurements; Molecular Sequence Data; Peptides; Protein Binding; Sensitivity and Specificity | 1996 |
Development of a sensitive enzymeimmunoassay for oxytocin determination in bovine plasma.
Topics: Animals; Antibody Formation; Biotin; Cattle; Chromatography, Gel; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Female; Immune Sera; Immunoenzyme Techniques; Immunoglobulin G; Lactation; Oxytocin; Rabbits; Radioimmunoassay; Reproducibility of Results; Sensitivity and Specificity | 1998 |
Detection of protein tyrosine kinase activity using a high-capacity streptavidin-coated membrane and optimized biotinylated peptide substrates.
Topics: Amino Acid Sequence; Animals; Biotin; Chemistry Techniques, Analytical; CHO Cells; Cricetinae; ErbB Receptors; In Vitro Techniques; Kinetics; Membranes, Artificial; Oligopeptides; Peptides; Protein-Tyrosine Kinases; Sensitivity and Specificity; Streptavidin; Substrate Specificity | 1998 |
A rapid and sensitive assay for histone acetyl-transferase activity.
Topics: 3T3 Cells; Acetyl Coenzyme A; Acetylation; Acetyltransferases; Amino Acid Sequence; Animals; Biotin; Carbon Radioisotopes; Histone Acetyltransferases; Histones; Mice; Molecular Sequence Data; Peptide Fragments; Recombinant Fusion Proteins; Saccharomyces cerevisiae Proteins; Sensitivity and Specificity; Streptavidin; Substrate Specificity | 1998 |
Discrimination of Bordetella parapertussis and Bordetella pertussis organisms from clinical isolates by PCR using biotin-labelled oligonucleotide probes.
Topics: Biotin; Bordetella; Bordetella Infections; Bordetella pertussis; Humans; Oligonucleotide Probes; Polymerase Chain Reaction; Predictive Value of Tests; Sensitivity and Specificity; Whooping Cough | 1998 |
Development of a PCR microplate-capture hybridization method for simple, fast and sensitive detection of Salmonella serovars in food.
Topics: Biotin; Blotting, Southern; DNA, Complementary; Food Microbiology; Nucleic Acid Hybridization; Polymerase Chain Reaction; Reproducibility of Results; Salmonella; Sensitivity and Specificity; Streptavidin | 1998 |
Mass-sensing, multianalyte microarray immunoassay with imaging detection.
Topics: Avidin; Biotin; Fluorescence; Fluorescent Dyes; Humans; Image Enhancement; Immunoassay; Immunoglobulin G; Miniaturization; Sensitivity and Specificity | 1998 |
Immunoglobulin M-capture biotin-streptavidin enzyme-linked immunosorbent assay for detection of antibodies to dengue viruses.
Topics: Acute Disease; Adolescent; Adult; Antibodies, Viral; Biotin; Convalescence; Dengue; Dengue Virus; Enzyme-Linked Immunosorbent Assay; Hemagglutination Inhibition Tests; Humans; Immunoglobulin G; Immunoglobulin M; Indicators and Reagents; Sensitivity and Specificity; Streptavidin | 1998 |
Sensitive mRNA detection by fluorescence in situ hybridization using horseradish peroxidase-labeled oligodeoxynucleotides and tyramide signal amplification.
Topics: Biotin; Blotting, Northern; Cytokines; Horseradish Peroxidase; Humans; In Situ Hybridization, Fluorescence; Oligonucleotides; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sensitivity and Specificity; Tumor Cells, Cultured; Tyramine | 1998 |
In situ localization of PCR-amplified DNA and cDNA.
Topics: Biotin; Digoxigenin; DNA, Complementary; DNA, Viral; Endopeptidase K; Female; Gene Dosage; HeLa Cells; Hepatitis C; Humans; In Situ Hybridization; Liver; Measles; Papillomaviridae; Polymerase Chain Reaction; Sensitivity and Specificity; Tumor Cells, Cultured; Uterine Cervical Neoplasms | 1998 |
In situ hybridization detection of low copy nucleic acid sequences using catalyzed reporter deposition and its usefulness in clinical human papillomavirus typing.
Topics: 3,3'-Diaminobenzidine; Biotin; Biotinylation; DNA Probes, HPV; DNA, Viral; Fluorescein; Formaldehyde; HeLa Cells; Horseradish Peroxidase; Humans; In Situ Hybridization; Papillomaviridae; Papillomavirus Infections; Paraffin Embedding; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Tissue Fixation; Tumor Cells, Cultured; Tumor Virus Infections; Tyramine | 1998 |
Biotin determination by three different methods: specificity and application to urine and plasma ultrafiltrates of patients with and without disorders in biotin metabolism.
Topics: Administration, Oral; Biotin; Carbon-Carbon Ligases; Cells, Cultured; Child; Chromatography, High Pressure Liquid; Enzyme Activation; Fibroblasts; Humans; Metabolism, Inborn Errors; Sensitivity and Specificity; Ultrafiltration | 1998 |
Detection of cytokine gene expression in human monocytes and lymphocytes by fluorescent in situ hybridization in cell suspension and flow cytometry.
Topics: Biotin; Digoxigenin; DNA Probes; Flow Cytometry; Gene Expression; Humans; In Situ Hybridization, Fluorescence; Kinetics; Leukocytes, Mononuclear; Lipopolysaccharides; Lymphocytes; Monocytes; Ribonucleases; RNA, Messenger; Sensitivity and Specificity; Sepsis; Stomach Neoplasms; Tumor Necrosis Factor-alpha | 1998 |
Ultrastructural localization of beta-actin and amphoterin mRNA in cultured cells: application of tyramide signal amplification and comparison of detection methods.
Topics: Actins; Animals; Biomarkers, Tumor; Biotin; Carrier Proteins; Coloring Agents; Cytoskeleton; High Mobility Group Proteins; HMGB1 Protein; In Situ Hybridization; Microscopy, Electron; Microscopy, Fluorescence; p-Dimethylaminoazobenzene; Pseudopodia; Rats; RNA, Messenger; Sensitivity and Specificity; Tumor Cells, Cultured; Tyramine | 1999 |
Telomerase assay using biotinylated-primer extension and magnetic separation of the products.
Topics: Biomarkers, Tumor; Biotin; Clinical Enzyme Tests; DNA Primers; DNA, Neoplasm; Guanosine Triphosphate; Humans; Magnetics; Microspheres; Sensitivity and Specificity; Telomerase; Telomere; Temperature | 1998 |
Nonradioactive Northern blot analysis of plant RNA and the application of different haptens for reprobing.
Topics: Biotin; Blotting, Northern; Digoxigenin; Fluorescein; Genes, Plant; Haptens; Hordeum; Nucleic Acid Hybridization; Plant Leaves; Polymerase Chain Reaction; Radioactive Tracers; RNA Probes; RNA, Antisense; RNA, Messenger; RNA, Plant; Sensitivity and Specificity; Templates, Genetic; Transcription, Genetic | 1998 |
Biotinylated enzyme inhibitorsorbent assay: a specific method for quantitating enzyme and its inhibitor.
Topics: Biotin; Enzyme Inhibitors; Enzymes; Hot Temperature; Sensitivity and Specificity; Trypsin | 1998 |
[THe use of a biotin-labelled DNA probe for detecting the causative agents of chronic urogenital infections].
Topics: Biotin; Chronic Disease; DNA Probes; DNA, Bacterial; DNA, Viral; Epithelial Cells; Female; Humans; Immunoblotting; Male; Nucleic Acid Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity; Urinary Tract Infections | 1998 |
Application of biotinylated tyramine to amplify reactions with horseradish peroxidase labelling.
Topics: Antigen-Antibody Reactions; Biotin; Breast Neoplasms; Epidermis; Female; Horseradish Peroxidase; Humans; Immunoenzyme Techniques; In Situ Hybridization; In Situ Nick-End Labeling; Keratins; Receptors, Estrogen; Sensitivity and Specificity; Tyramine | 1999 |
Clinical validation of the avidin/indium-111 biotin approach for imaging infection/inflammation in orthopaedic patients.
Topics: Avidin; Biotin; Discitis; Female; Humans; Indium Radioisotopes; Leukocytes; Male; Middle Aged; Osteomyelitis; Predictive Value of Tests; Prosthesis-Related Infections; Radionuclide Imaging; Radiopharmaceuticals; Reproducibility of Results; Sensitivity and Specificity; Technetium Tc 99m Exametazime | 1999 |
Detection of endogenous biotin in various tissues: novel functions in the hippocampus and implications for its use in avidin-biotin technology.
Topics: Animals; Avidin; Biotin; False Positive Reactions; Female; Hippocampus; Immunohistochemistry; Indicators and Reagents; Male; Organ Specificity; Rats; Sensitivity and Specificity | 1999 |
Development of a sensitive enzymeimmunoassay (EIA) for FSH determination in bovine plasma.
Topics: Animals; Biotin; Cattle; Estrus; Female; Follicle Stimulating Hormone; Immunoenzyme Techniques; Immunoglobulin G; Luteinizing Hormone; Prostaglandins F; Radioimmunoassay; Sensitivity and Specificity; Streptavidin | 1999 |
Application of tyramide signal amplification system to immunohistochemistry: a potent method to localize antigens that are not detectable by ordinary method.
Topics: Antibodies; Antigens, CD; Biotin; Blotting, Western; Heart Neoplasms; Hepatitis C; Humans; Immunohistochemistry; Ki-67 Antigen; Mycosis Fungoides; Palatine Tonsil; Pericardium; Sensitivity and Specificity; Tyramine | 1999 |
Specific bio-recognition reactions observed with an integrated Mach-Zehnder interferometer.
Topics: Biosensing Techniques; Biotin; Interferometry; Miniaturization; Refractometry; Sensitivity and Specificity; Silicon; Streptavidin | 1999 |
Development and evaluation of a competitive time-resolved immunofluorometric assay for the estrogen-regulated protein pS2.
Topics: Aldosterone; Amino Acid Sequence; Antibodies, Monoclonal; Binding, Competitive; Biotin; Breast Neoplasms; Calibration; Chemistry, Clinical; Dexamethasone; Dihydrotestosterone; Dose-Response Relationship, Immunologic; Drosophila Proteins; Estradiol; Evaluation Studies as Topic; Fluorescent Antibody Technique; Fluorometry; Glucocorticoids; Integrin alpha Chains; Integrins; Molecular Sequence Data; Nandrolone; Norgestrel; Progesterone Congeners; Sensitivity and Specificity; Testosterone Congeners; Tumor Cells, Cultured | 1999 |
Human papillomavirus detection by non isotopic in situ hybridization, in situ hybridization with signal amplification and in situ polymerase chain reaction.
Topics: Biotin; Female; HeLa Cells; Humans; In Situ Hybridization; Papillomaviridae; Polymerase Chain Reaction; Sensitivity and Specificity; Tumor Cells, Cultured; Tyramine; Uterine Cervical Dysplasia | 1999 |
A.E. Bennett Research Award. Anatomic basis for differential regulation of the rostrolateral peri-locus coeruleus region by limbic afferents.
Topics: Animals; Awards and Prizes; Biotin; Cell Count; Corticotropin-Releasing Hormone; Dendrites; Dextrans; Electrophysiology; Fluorescent Dyes; Immune Sera; Intralaminar Thalamic Nuclei; Limbic System; Locus Coeruleus; Male; Neurons, Afferent; Presynaptic Terminals; Rats; Rats, Sprague-Dawley; Research; Sensitivity and Specificity; Septal Nuclei; Tyrosine 3-Monooxygenase; Up-Regulation | 1999 |
Screening unlabeled DNA targets with randomly ordered fiber-optic gene arrays.
Topics: Base Sequence; Binding Sites; Biotin; Cystic Fibrosis Transmembrane Conductance Regulator; DNA; Fiber Optic Technology; Fluorescent Dyes; Humans; Microscopy, Fluorescence; Microspheres; Molecular Probe Techniques; Mutation; Nucleic Acid Conformation; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Oligonucleotide Probes; Optical Fibers; Polymerase Chain Reaction; Random Allocation; Sensitivity and Specificity; Time Factors | 2000 |
Specific in situ hybridization of Haemobartonella felis with a DNA probe and tyramide signal amplification.
Topics: Anaplasmataceae; Anaplasmataceae Infections; Anemia; Animals; Bacteremia; Biotin; Cat Diseases; Cats; Colorimetry; DNA Probes; In Situ Hybridization; Kidney; Liver; Polymerase Chain Reaction; RNA, Bacterial; RNA, Ribosomal, 16S; Sensitivity and Specificity; Tyramine | 2000 |
High-density hapten labeling and HRP conjugation of oligonucleotides for use as in situ hybridization probes to detect mRNA targets in cells and tissues.
Topics: Biotin; Chorionic Villi; Digoxigenin; Fetus; Fluorescein; Haptens; Hemoglobins; Horseradish Peroxidase; Humans; In Situ Hybridization; Liver; Oligonucleotide Probes; Sensitivity and Specificity | 2000 |
A simple and rapid technique for the detection of Epstein-Barr virus DNA in HIV-associated oral hairy leukoplakia biopsies.
Topics: AIDS-Related Opportunistic Infections; Biotin; Case-Control Studies; DNA Probes; DNA, Viral; Female; Herpesvirus 4, Human; HIV Seropositivity; Humans; In Situ Hybridization; In Situ Nick-End Labeling; Leukoplakia, Hairy; Male; Random Amplified Polymorphic DNA Technique; Sensitivity and Specificity | 2000 |
Rapid diagnosis of acute pyogenic meningitis by a combined PCR dot-blot assay.
Topics: Biotin; Cerebrospinal Fluid; DNA, Bacterial; Double-Blind Method; Evaluation Studies as Topic; Haemophilus influenzae; Humans; Immunoblotting; In Situ Hybridization; Meningitis, Bacterial; Neisseria meningitidis; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity; Streptococcus pneumoniae | 2000 |
Identification of Staphylococcus aureus enterotoxins A and B genes by PCR-ELISA.
Topics: Bacteria; Biotin; Colorimetry; Cross Reactions; DNA Probes; Enterotoxins; Enzyme-Linked Immunosorbent Assay; Polymerase Chain Reaction; Sensitivity and Specificity; Staphylococcus aureus | 2000 |
Application of polymer-embedded proteins to fabrication of DNA array.
Topics: Adsorption; Bacterial Toxins; Biotin; Biotinylation; DNA; Fluorescence; Glass; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Oligonucleotide Probes; Polylysine; Polymers; Protein Binding; Sensitivity and Specificity; Shiga Toxin 2; Siloxanes; Streptavidin; Surface Properties | 2000 |
A highly sensitive nonisotopic detection method for in situ hybridization.
Topics: Animals; Biotin; DNA Probes, HPV; DNA, Viral; Evaluation Studies as Topic; Female; Humans; In Situ Hybridization; Mice; Papillomaviridae; Sensitivity and Specificity; Tumor Cells, Cultured; Uterine Cervical Neoplasms | 2000 |
High-performance liquid chromatography coupled to enzyme-amplified biochemical detection for the analysis of hemoglobin after pre-column biotinylation.
Topics: Alkaline Phosphatase; Biotin; Calibration; Chromatography, High Pressure Liquid; Hemoglobins; Humans; Reproducibility of Results; Sensitivity and Specificity | 2000 |
Leishmania antigen nonspecific binding to avidin in biotin-avidin immunoassays.
Topics: Animals; Antigens, Protozoan; Avidin; Biotin; Humans; Immunoassay; Leishmania; Leishmaniasis; Sensitivity and Specificity | 2000 |
Determination of tumor necrosis factor-alpha levels in dengue virus infected patients by sensitive biotin-streptavidin enzyme-linked immunosorbent assay.
Topics: Adolescent; Biotin; Child; Child, Preschool; Dengue; Enzyme-Linked Immunosorbent Assay; Humans; Recombinant Proteins; Sensitivity and Specificity; Severe Dengue; Severity of Illness Index; Streptavidin; Tumor Necrosis Factor-alpha | 2000 |
Polyvinylamine-streptavidin complexes labeled with a europium chelator: a universal detection reagent for solid-phase time resolved fluorometric applications.
Topics: Animals; Biotin; Calibration; Chelating Agents; Chromatography, High Pressure Liquid; Dose-Response Relationship, Drug; Europium; Fluorescent Dyes; Fluoroimmunoassay; Fluorometry; Indicators and Reagents; Mice; Phenanthrolines; Polyvinyls; Sensitivity and Specificity; Streptavidin; Time Factors | 2000 |
Determination of matrix metalloproteinase activity using biotinylated gelatin.
Topics: Binding Sites; Biotin; Biotinylation; Chemistry, Clinical; DNA, Complementary; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Gelatin; Horseradish Peroxidase; Humans; Hydroxamic Acids; Indoles; Kinetics; Lysine; Matrix Metalloproteinase 2; Matrix Metalloproteinase 8; Matrix Metalloproteinases; Protease Inhibitors; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Time Factors; Tumor Cells, Cultured | 2000 |
A series of biotinylated tracers distinguishes three types of gap junction in retina.
Topics: Animals; Benzimidazoles; Biotin; Biotinylation; Fluorescent Dyes; Gap Junctions; Histocytochemistry; Indoles; Iontophoresis; Microinjections; Nerve Net; Octanols; Permeability; Rabbits; Retina; Sensitivity and Specificity; Streptavidin | 2000 |
Tyramide signal amplification of biotinylated probe in dot-blot hybridization assay for the detection of parvovirus B19 DNA in serum samples.
Topics: Biotin; Biotinylation; DNA Probes; DNA, Viral; Enzyme-Linked Immunosorbent Assay; Humans; Nucleic Acid Hybridization; Parvoviridae Infections; Parvovirus B19, Human; Polymerase Chain Reaction; Sensitivity and Specificity | 2000 |
Clinical relevance of HPV 16/18 testing methods in cervical squamous cell carcinoma.
Topics: Adult; Aged; Aged, 80 and over; Biotin; Carcinoma, Squamous Cell; Disease-Free Survival; DNA, Complementary; Female; Follow-Up Studies; Humans; In Situ Hybridization; Indicators and Reagents; Middle Aged; Papillomaviridae; Peroxidase; Reproducibility of Results; Sensitivity and Specificity; Silver; Streptavidin; Time Factors; Tyramine; Uterine Cervical Neoplasms | 2000 |
Development and evaluation of an IgM-capture ELISA for detection of recent infection with bluetongue viruses in cattle.
Topics: Animals; Antibodies, Monoclonal; Antibodies, Viral; Antibody Specificity; Antigens, Viral; Avidin; Binding, Competitive; Biotin; Bluetongue; Bluetongue virus; Cattle; Cattle Diseases; Enzyme-Linked Immunosorbent Assay; Immunoglobulin M; Sensitivity and Specificity; Viral Core Proteins | 2001 |
Serologic detection of Actinobacillus pleuropneumoniae in swine by capsular polysaccharide-biotin-streptavidin enzyme-linked immunosorbent assay.
Topics: Actinobacillus Infections; Actinobacillus pleuropneumoniae; Animals; Antibodies, Bacterial; Bacterial Capsules; Biotin; Biotinylation; Enzyme-Linked Immunosorbent Assay; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Swine; Swine Diseases | 2001 |
Nearly instantaneous, cation-independent, high selectivity nucleic acid hybridization to DNA microarrays.
Topics: Biotin; Cations; Fluorescent Dyes; Hydrogen-Ion Concentration; Molecular Chaperones; Nucleic Acid Conformation; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Oligonucleotides; Osmolar Concentration; Polystyrenes; Sensitivity and Specificity; Streptavidin; Surface Properties; Time Factors | 2001 |
Development of highly fluorescent detection reagents for the construction of ultrasensitive immunoassays.
Topics: Biotin; Chelating Agents; Europium; Fluorescent Dyes; Fluorometry; Immunoassay; Indicators and Reagents; Metals, Rare Earth; Polyglutamic Acid; Polylysine; Prostate-Specific Antigen; Sensitivity and Specificity; Streptavidin | 2001 |
HHV-8 ELISA based on a one-step affinity capture of biotinylated K8.1 antigen.
Topics: Biotin; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique, Indirect; Gene Expression; Glycoproteins; Herpesvirus 8, Human; Humans; Recombinant Fusion Proteins; Sarcoma, Kaposi; Sensitivity and Specificity; Tumor Cells, Cultured; Viral Proteins | 2001 |
Matrix interference in serum total thyroxin (T4) time-resolved fluorescence immunoassay (TRFIA) and its elimination with the use of streptavidin-biotin separation technique.
Topics: Antibodies, Monoclonal; Binding, Competitive; Biotin; Coated Materials, Biocompatible; Equipment Design; Fluoroimmunoassay; Humans; Immobilization; Immunoassay; Sensitivity and Specificity; Serum Albumin, Bovine; Streptavidin; Thyroxine | 2001 |
Development of a sensitive and specific new plasma 4-androstene-3,17- dione time-resolved fluoroimmunoassay (TR-FIA).
Topics: Adolescent; Adult; Androstenedione; Animals; Biotin; Child; Chromatography; Dose-Response Relationship, Drug; Europium; Female; Fluoroimmunoassay; Humans; Male; Models, Chemical; Rabbits; Radioimmunoassay; Reproducibility of Results; Sensitivity and Specificity | 2001 |
A direct streptavidin-binding assay does not accurately quantitate biotin in human urine.
Topics: Avidin; Biotin; Chromatography, High Pressure Liquid; Female; Fluorescence; Humans; Indicators and Reagents; Male; Protein Binding; Regression Analysis; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2001 |
Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species.
Topics: Antibodies, Monoclonal; Biosensing Techniques; Biotin; Boron; Calcium; Calmodulin; Chemistry Techniques, Analytical; Electric Conductivity; Hydrogen-Ion Concentration; Ligands; Miniaturization; Proteins; Protons; Semiconductors; Sensitivity and Specificity; Silicon; Streptavidin; Surface Properties | 2001 |
Hotwiring biosensors.
Topics: Antibodies, Monoclonal; Biosensing Techniques; Biotin; Calcium; Chemistry Techniques, Analytical; Miniaturization; Propylamines; Sensitivity and Specificity; Silanes; Silicon; Technology | 2001 |
DNA sequencing using biotinylated dideoxynucleotides and mass spectrometry.
Topics: Base Sequence; Biotin; Biotinylation; Dideoxynucleosides; DNA; Heterozygote; Microspheres; Molecular Sequence Data; Mutation; Polymorphism, Genetic; Sensitivity and Specificity; Sequence Analysis, DNA; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Streptavidin; Templates, Genetic | 2001 |
Detection of epitope-tagged proteins in flow cytometry: fluorescence resonance energy transfer-based assays on beads with femtomole resolution.
Topics: Antibodies; Biosensing Techniques; Biotin; Energy Transfer; Epitopes; Flow Cytometry; Fluorescence; Kinetics; Microspheres; Oligopeptides; Peptides; Rhodamines; Sensitivity and Specificity; Spectrometry, Fluorescence; Streptavidin | 2001 |
Determination of human tumor necrosis factor alpha by a highly sensitive enzyme immunoassay.
Topics: Biotin; Blood Chemical Analysis; Enzyme-Linked Immunosorbent Assay; Horseradish Peroxidase; Humans; Immunoenzyme Techniques; Reference Standards; Sensitivity and Specificity; Streptavidin; Tumor Necrosis Factor-alpha | 2001 |
A new avidin-biotin optical immunoassay for the detection of beta-bungarotoxin and application in diagnosis of experimental snake envenomation.
Topics: Animals; Avidin; Biotin; Bungarotoxins; Immunoassay; Mice; Rabbits; Reagent Kits, Diagnostic; Sensitivity and Specificity; Snake Bites | 2002 |
A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface.
Topics: Biosensing Techniques; Biotin; Gold Colloid; Ligands; Nanotechnology; Sensitivity and Specificity; Spectrum Analysis; Streptavidin | 2002 |
Electron beam patterning of biomolecules.
Topics: Biotin; Computer Simulation; Electron Probe Microanalysis; Electrons; Feasibility Studies; Nanotechnology; Photography; Radiation Dosage; Scattering, Radiation; Sensitivity and Specificity; Silicon | 2002 |
Multicenter evaluation of a new immunoassay for intact PTH measurement on the Elecsys System 2010 and 1010.
Topics: Adolescent; Adult; Antibodies, Monoclonal; Biotin; Female; Humans; Immunoassay; Male; Middle Aged; Parathyroid Hormone; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 2002 |
Determination of 3,5,6-trichloro-2-pyridinol (TCP) in water by a continuous competitive immunoassay system based on the streptavidin-biotin interaction.
Topics: Antibodies, Monoclonal; Binding, Competitive; Biotin; Environmental Monitoring; Herbicides; Immunoassay; Insecticides; Pyridones; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Water; Water Pollutants, Chemical; Water Supply | 2002 |
Ultrasensitive optical DNA biosensor based on surface immobilization of molecular beacon by a bridge structure.
Topics: Biosensing Techniques; Biotin; DNA Probes; Fiber Optic Technology; Fluorescent Dyes; Molecular Structure; Oligonucleotide Probes; p-Dimethylaminoazobenzene; Rhodamines; Sensitivity and Specificity; Streptavidin; Surface Properties | 2001 |
Detection of biotinylated proteins in polyacrylamide gels using an avidin-fluorescein conjugate.
Topics: Avidin; Bacterial Proteins; Biotin; Biotinylation; Carbon-Nitrogen Ligases; Cell Culture Techniques; Centrifugation; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Fluoresceins; Glutathione Transferase; Humans; Immunoblotting; Immunoglobulin Heavy Chains; Immunoglobulin Variable Region; Membrane Proteins; Polymerase Chain Reaction; Proteins; Repressor Proteins; Sensitivity and Specificity; Silver Staining; Transcription Factors | 2002 |
Time-resolved immunofluorometry of serum hTSH with enhanced sensitivity.
Topics: Animals; Antibodies, Monoclonal; Antibody Specificity; Biotin; Cross Reactions; Europium; Fluoroimmunoassay; Goats; Humans; Hyperthyroidism; Immunoglobulin G; Iodine Radioisotopes; Luminescent Measurements; Mice; Pentetic Acid; Sensitivity and Specificity; Streptavidin; Thyrotropin | 2002 |
Sensitivity studies for specific binding reactions using the biotin/streptavidin system by evanescent optical methods.
Topics: Antigen-Antibody Reactions; Biotin; Interferometry; Microscopy, Atomic Force; Optics and Photonics; Sensitivity and Specificity; Streptavidin; Surface Plasmon Resonance | 2002 |
Lymphocyte propionyl-CoA carboxylase is an early and sensitive indicator of biotin deficiency in rats, but urinary excretion of 3-hydroxypropionic acid is not.
Topics: Animals; Biomarkers; Biotin; Carboxy-Lyases; Deficiency Diseases; Glycine; Lactic Acid; Lymphocytes; Male; Methylmalonyl-CoA Decarboxylase; Rats; Rats, Sprague-Dawley; Sensitivity and Specificity | 2002 |
Streptavidin-based quantitative staining of intracellular antigens for flow cytometric analysis.
Topics: 3T3 Cells; Animals; Antigens; Antigens, Polyomavirus Transforming; Bacterial Proteins; Biotin; Flow Cytometry; Mice; Recombinant Fusion Proteins; Sensitivity and Specificity; Staining and Labeling; Streptavidin | 1992 |
A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates.
Topics: Avian Leukosis Virus; Avian Myeloblastosis Virus; Biotin; Deoxyuracil Nucleotides; HIV-1; HIV-2; Oligodeoxyribonucleotides; Poly A; Retroviridae; RNA-Directed DNA Polymerase; Sensitivity and Specificity; Templates, Genetic; Virology | 1992 |
Method for the detection of glycopeptides at the picomole level in HPLC peptide maps.
Topics: alpha-Fetoproteins; Amino Acid Sequence; Asialoglycoproteins; Biotin; Carbohydrate Sequence; Chromatography, High Pressure Liquid; Fetuins; Glycopeptides; Glycosylation; Lectins; Microchemistry; Molecular Sequence Data; Ovalbumin; Peptide Fragments; Peptide Mapping; Peroxidases; Polysaccharides; Sensitivity and Specificity | 1992 |
Site-specific biotinylation. A novel approach and its application to endothelin-1 analogs and PTH-analog.
Topics: Amino Acid Sequence; Binding Sites; Biotin; Endothelins; Molecular Sequence Data; Oxidation-Reduction; Parathyroid Hormone; Peptide Fragments; Receptors, Endothelin; Sensitivity and Specificity | 1992 |
Development of a biotin-streptavidin-enhanced enzyme-linked immunosorbent assay which uses monoclonal antibodies for detection of group C rotaviruses.
Topics: Animals; Antibodies, Monoclonal; Bacterial Proteins; Binding, Competitive; Biotin; Cattle; Child; Enzyme-Linked Immunosorbent Assay; Feces; Humans; Rotavirus; Sensitivity and Specificity; Streptavidin; Swine | 1992 |
Characterization of nonradioactive hybridization probes for detecting infectious bursal disease virus.
Topics: Animals; Base Sequence; Biotin; Chickens; Cloning, Molecular; DNA Probes; DNA, Viral; Infectious bursal disease virus; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; Poultry Diseases; Reoviridae Infections; RNA, Viral; Sensitivity and Specificity; Transcription, Genetic | 1992 |
Detection of Epstein-Barr virus genome in Ki-1 (CD30)-positive, large-cell anaplastic lymphomas using the polymerase chain reaction.
Topics: Antigens, CD; Antigens, Neoplasm; Biotin; DNA Probes; Genome, Viral; Herpesvirus 4, Human; Humans; Ki-1 Antigen; Lymphoma, Large B-Cell, Diffuse; Nucleic Acid Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity; Sulfur Radioisotopes | 1992 |
Quantitation of polymerase chain reaction products by hybridization-based assays with fluorescent, colorimetric, or chemiluminescent detection.
Topics: Bacterial Proteins; Base Sequence; Biotin; Colorimetry; DNA; DNA, Viral; Feasibility Studies; Flow Cytometry; Globins; Herpesvirus 4, Human; Humans; Immunoenzyme Techniques; Luminescent Measurements; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity; Spectrometry, Fluorescence; Streptavidin | 1992 |
Specific diagnosis of parvovirus enteritis in dogs and cats by in situ hybridization.
Topics: Animals; Antigens, Viral; Biotin; Cats; DNA Probes; DNA, Viral; Dog Diseases; Dogs; Enteritis; Feline Panleukopenia; Feline Panleukopenia Virus; Immunoenzyme Techniques; In Situ Hybridization; Intestines; Parvoviridae; Parvoviridae Infections; Sensitivity and Specificity | 1992 |
A sensitive method to detect cell surface receptors using biotinylated growth factors.
Topics: Animals; Avidin; Biotin; Cell Division; Cells, Cultured; Flow Cytometry; Fluorescent Antibody Technique; Granulocyte-Macrophage Colony-Stimulating Factor; Hematopoietic Cell Growth Factors; Hematopoietic Stem Cells; Interleukin-3; Receptors, Cell Surface; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; Receptors, Interleukin-3; Recombinant Proteins; Sensitivity and Specificity | 1992 |
Novel and ultrasensitive noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay) for alpha-human atrial natriuretic peptide.
Topics: Atrial Natriuretic Factor; Binding Sites; Biotin; Humans; Immunoenzyme Techniques; Peptide Fragments; Reference Standards; Sensitivity and Specificity | 1992 |
Quantification of sensitive non-isotopic filter hybridizations using the peroxidase catalyzed luminol reaction.
Topics: Biotin; Biphenyl Compounds; Blotting, Southern; Dextran Sulfate; Digoxigenin; DNA; Firefly Luciferin; Horseradish Peroxidase; Iodobenzenes; Luminescent Measurements; Luminol; Nucleic Acid Hybridization; Photography; Polyethylene Glycols; Reproducibility of Results; Sensitivity and Specificity; Thiazoles; Ultrafiltration | 1992 |
Peanut agglutinin (lectin from Arachis hypogaea) binding to hemopoietic cells: an immunophenotypic study using a biotin streptavidin technique.
Topics: Bacterial Proteins; Biotin; Bone Marrow Purging; Cell Line; Hematopoietic Stem Cells; Humans; Immunophenotyping; Lectins; Leukemia; Lymphoma; Macrophages; Monocytes; Multiple Myeloma; Peanut Agglutinin; Plasma Cells; Sensitivity and Specificity; Streptavidin | 1992 |
An automated biotin-streptavidin procedure for progesterone evaluation.
Topics: Autoanalysis; Bacterial Proteins; Biotin; Calibration; Humans; Immunoassay; Progesterone; Radioimmunoassay; Reference Values; Regression Analysis; Sensitivity and Specificity; Streptavidin | 1992 |
Rapid, non-separation electrochemiluminescent DNA hybridization assays for PCR products, using 3'-labelled oligonucleotide probes.
Topics: 2,2'-Dipyridyl; Biotin; Blood Proteins; Calgranulin A; Cystic Fibrosis; DNA; Fluorescent Dyes; Genes, gag; Genes, Synthetic; HIV-1; Humans; Incidence; Luminescent Measurements; Nucleic Acid Hybridization; Oligonucleotide Probes; Organometallic Compounds; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity | 1992 |
A comparative study of avidin-biotin-peroxidase complexes for the immunohistochemical detection of antigens in neural tissue.
Topics: Animals; Antibodies; Antigens; Avidin; Bacterial Proteins; Biotin; Brain; Brain Chemistry; Immunohistochemistry; Male; Parvalbumins; Peroxidases; Rats; Rats, Wistar; Reagent Kits, Diagnostic; Sensitivity and Specificity; Streptavidin | 1992 |
Detection of African horsesickness virus in infected spleens by a sandwich ELISA using two monoclonal antibodies specific for VP7.
Topics: African Horse Sickness; African Horse Sickness Virus; Animals; Antibodies, Monoclonal; Antibodies, Viral; Antigens, Viral; Avidin; Biotin; Capsid; Capsid Proteins; Enzyme-Linked Immunosorbent Assay; Horses; Sensitivity and Specificity; Spleen | 1992 |
Determination of prostaglandin metabolites in biological samples by competitive time-resolved fluoroimmunoassay.
Topics: Bacterial Proteins; Binding, Competitive; Biotin; Breast Neoplasms; Dinoprost; Dinoprostone; Europium; Fluoroimmunoassay; Humans; Prostaglandins; Radioimmunoassay; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 1992 |
Cystatin C levels in sera of patients with human immunodeficiency virus infection. A new avidin-biotin ELISA assay for its measurement.
Topics: Adolescent; Adult; Avidin; beta 2-Microglobulin; Biomarkers; Biotin; Child; Cystatins; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Female; HIV Infections; Humans; Male; Middle Aged; Sensitivity and Specificity; Time Factors | 1992 |
Nonradioactive in situ hybridization with digoxigenin labeled DNA probes.
Topics: Animals; Biotin; Cell Nucleus; Chromosomes; Digoxigenin; DNA Probes; Humans; Liver Neoplasms, Experimental; Nucleic Acid Hybridization; Sensitivity and Specificity; Tumor Cells, Cultured; Y Chromosome | 1992 |
Chemiluminescent microtiter method for detecting PCR amplified HIV-1 DNA.
Topics: Alkaline Phosphatase; Bacterial Proteins; Base Sequence; Biotin; DNA, Viral; HIV-1; Humans; Luminescent Measurements; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 1992 |
Detection and typing of human papillomaviruses in cervical smears by an original application of the polymerase chain reaction.
Topics: Avidin; Base Sequence; Biotin; Blotting, Southern; Cervix Uteri; DNA Probes; DNA, Viral; Female; Humans; Iodine; Isotope Labeling; Microspheres; Molecular Sequence Data; Nucleic Acid Hybridization; Papillomaviridae; Polymerase Chain Reaction; Prevalence; Sensitivity and Specificity; Tumor Virus Infections; Uterine Cervical Neoplasms; Vaginal Smears | 1991 |
Detection of amplified Wuchereria bancrofti DNA in mosquitoes with a nonradioactive probe.
Topics: Animals; Base Sequence; Biotin; Culex; Culicidae; DNA; Larva; Luminescent Measurements; Molecular Sequence Data; Oligonucleotides; Polymerase Chain Reaction; Sensitivity and Specificity; Species Specificity; Wuchereria bancrofti | 1991 |
C-myc expression in early human placenta--a critical evaluation of its localization.
Topics: Alkaline Phosphatase; Antibodies, Monoclonal; Biotin; Female; Gene Expression; Genes, myc; Histocytochemistry; Humans; Immunohistochemistry; Nucleic Acid Hybridization; Placenta; Pregnancy; Pregnancy Trimester, First; Protein Biosynthesis; Sensitivity and Specificity; Transcription, Genetic | 1991 |
ABC-immunoperoxidase staining of cytologic preparations: improvement of specificity.
Topics: Avidin; Biotin; False Positive Reactions; Histocytochemistry; Humans; Immunoenzyme Techniques; Liver; p-Dimethylaminoazobenzene; Sensitivity and Specificity; Staining and Labeling | 1990 |
Analysis of RNA stability and (-) strand content in viral infections using biotinylated RNA probes.
Topics: Biotin; Blotting, Northern; Densitometry; DNA, Viral; Mosaic Viruses; Mutation; Plants; Plasmids; Protoplasts; RNA; RNA Probes; RNA, Complementary; RNA, Viral; Sensitivity and Specificity; Virus Replication | 1990 |
Rapid serotyping of infectious pancreatic necrosis virus by one-step enzyme-linked immunosorbent assay using monoclonal antibodies.
Topics: Animals; Antibodies, Monoclonal; Antigens, Viral; Biotin; Enzyme-Linked Immunosorbent Assay; Epitopes; Fish Diseases; Fishes; Reoviridae; Sensitivity and Specificity; Serotyping | 1991 |
Quantification of nucleic acids on nitrocellulose membranes with time-resolved fluorometry.
Topics: Bacterial Proteins; Bacteriophage lambda; Biotin; Blotting, Southern; Collodion; DNA; DNA Probes; DNA, Viral; Fluorometry; Phenanthrolines; Sensitivity and Specificity; Staining and Labeling; Streptavidin | 1991 |
Rapid and biologically safe diagnosis of African swine fever virus infection by using polymerase chain reaction.
Topics: African Swine Fever; African Swine Fever Virus; Animals; Base Sequence; Biotin; Cells, Cultured; Cloning, Molecular; DNA, Viral; Macrophages; Molecular Sequence Data; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity; Species Specificity; Swine | 1992 |
Detection of human immunodeficiency virus type 1 infection in young pediatric patients by using polymerase chain reaction and biotinylated probes.
Topics: Acquired Immunodeficiency Syndrome; Age Factors; Biotin; Child; Child, Preschool; DNA Probes; DNA, Viral; Female; HIV-1; Humans; Infant; Infant, Newborn; Polymerase Chain Reaction; Sensitivity and Specificity | 1992 |
Diagnosis of invasive candidiasis by detection of mannan antigen by using the avidin-biotin enzyme immunoassay.
Topics: Antigens, Fungal; Avidin; Biotin; Candida; Candidiasis; Child; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Humans; Immunocompromised Host; Mannans; Sensitivity and Specificity; Species Specificity | 1991 |
Detection of astrovirus in pediatric stool samples by immunoassay and RNA probe.
Topics: Avidin; Biotin; Child, Preschool; Evaluation Studies as Topic; Feces; Humans; Immunoenzyme Techniques; Infant; Mamastrovirus; RNA Probes; Sensitivity and Specificity; Virus Diseases | 1991 |
Adsorption and biotin-streptavidin amplification in serologic tests for diagnosis of Lyme borreliosis.
Topics: Antibodies, Bacterial; Bacterial Proteins; Biotin; Borrelia burgdorferi Group; Enzyme-Linked Immunosorbent Assay; False Positive Reactions; Humans; Immunosorbent Techniques; Lyme Disease; Sensitivity and Specificity; Streptavidin | 1991 |
A biotin-streptavidin enzyme immunoassay for detection of antibodies to porcine granulosa cell antigens.
Topics: Animals; Antibodies; Antigens, Surface; Autoimmune Diseases; Bacterial Proteins; beta-Galactosidase; Biotin; Colorimetry; Enzyme-Linked Immunosorbent Assay; Female; Granulosa Cells; Humans; Ovariectomy; Polylysine; Primary Ovarian Insufficiency; Rabbits; Sensitivity and Specificity; Serum Albumin, Bovine; Streptavidin; Swine | 1991 |
A novel enzyme immunoassay for quantitation of rat prothrombin in microsomal subfractions.
Topics: Alkaline Phosphatase; Animals; Bacterial Proteins; Biotin; Endopeptidases; Enzyme-Linked Immunosorbent Assay; Immunoglobulin G; Microsomes, Liver; Prothrombin; Rabbits; Rats; Rats, Inbred Strains; Sensitivity and Specificity; Streptavidin | 1991 |
A microplate assay for analysis of solution-phase glycosyltransferase reactions: determination of kinetic constants.
Topics: Aequorin; Bacterial Proteins; Biotin; Calcium; Chorionic Gonadotropin; Galactosyltransferases; Humans; Kinetics; Lectins; Luminescent Measurements; N-Acetylgalactosaminyltransferases; Plant Lectins; Precipitin Tests; Receptors, N-Acetylglucosamine; Sensitivity and Specificity; Streptavidin | 1991 |
Comparison of reporter molecules for viral in situ hybridization.
Topics: Alkaline Phosphatase; Animals; Aviadenovirus; Bacterial Proteins; Biotin; Brain; Chickens; Fluoresceins; Genes, Viral; Gold; Humans; Nucleic Acid Hybridization; Nucleic Acid Probes; Peroxidases; Sensitivity and Specificity; SSPE Virus; Streptavidin | 1991 |
A comparison of non-radioactive labeling and detection systems with synthetic oligonucleotide probes for the species identification of mosquitoes in the Anopheles gambiae complex.
Topics: Animals; Anopheles; Base Sequence; Biotin; Costs and Cost Analysis; Digoxigenin; Horseradish Peroxidase; Insect Vectors; Luminescent Measurements; Male; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Sensitivity and Specificity | 1991 |
Production, characterisation and use of monoclonal antibodies to human interleukin-5 in an enzyme-linked immunosorbent assay.
Topics: Animals; Antibodies, Monoclonal; Biotin; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Female; Humans; Interleukin-5; Mice; Mice, Inbred BALB C; Mice, Inbred CBA; Rats; Sensitivity and Specificity | 1991 |
PCR driven DNA-DNA competitive hybridization: a new method for sensitive differential cloning.
Topics: Animals; Bacterial Proteins; Binding, Competitive; Biotin; Chick Embryo; Cloning, Molecular; DNA; Nucleic Acid Hybridization; Nucleotides; Polymerase Chain Reaction; Retina; Sensitivity and Specificity; Streptavidin | 1991 |
The construction of a monoclonal diagnostic system for the field detection of Vibrio cholerae.
Topics: Alkaline Phosphatase; Animals; Antibodies, Bacterial; Antibodies, Monoclonal; Avidin; Biotin; Collodion; Cross Reactions; Cross-Linking Reagents; Enzyme-Linked Immunosorbent Assay; Glutaral; Milk; Reagent Kits, Diagnostic; Sensitivity and Specificity; Vibrio cholerae | 1991 |
Improved immunoglobulin M serodiagnosis in Lyme borreliosis by using a mu-capture enzyme-linked immunosorbent assay with biotinylated Borrelia burgdorferi flagella.
Topics: Adolescent; Adult; Aged; Antigens, Bacterial; Binding, Competitive; Biotin; Borrelia burgdorferi Group; Child; Dermatitis; Enzyme-Linked Immunosorbent Assay; Erythema Chronicum Migrans; Female; Humans; Immunoglobulin M; Lyme Disease; Male; Middle Aged; Sensitivity and Specificity | 1991 |
Nonradioactive detection of nucleic acid by the universal probe system.
Topics: Biotin; DNA Probes; DNA, Single-Stranded; Gene Amplification; Globins; Humans; Plasmids; Proto-Oncogene Proteins c-myc; Restriction Mapping; Sensitivity and Specificity | 1991 |
Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase.
Topics: Biotin; Blotting, Southern; Digoxigenin; DNA; Haptens; Oligonucleotides; Sensitivity and Specificity; Transferases | 1991 |
Highly sensitive enzyme immunoassay for beta-nerve growth factor (NGF): a tool for measurement of NGF level in rat serum.
Topics: Animals; Avidin; Biotin; Evaluation Studies as Topic; Immunoenzyme Techniques; Nerve Growth Factors; Rats; Rats, Inbred Strains; Sensitivity and Specificity | 1990 |
Biotin and phosphorus-isotopic labelled DNA/RNA probes for the detection of human papilloma virus sequences.
Topics: Biotin; Blotting, Southern; Carcinoma in Situ; Carcinoma, Squamous Cell; DNA Probes; DNA Probes, HPV; Female; Genotype; Humans; Papillomaviridae; Phosphorus Isotopes; Predictive Value of Tests; RNA Probes; Sensitivity and Specificity; Uterine Cervical Neoplasms | 1990 |
Clinical assessment of specific enzyme immunoassay for the human cardiac myosin light chain II (MLC II) with use of monoclonal antibodies.
Topics: Adult; Aged; Antibodies, Monoclonal; Biomarkers; Biotin; Enzyme-Linked Immunosorbent Assay; Female; Humans; Male; Middle Aged; Myocardial Infarction; Myocardium; Myosins; Predictive Value of Tests; Reproducibility of Results; Sensitivity and Specificity | 1990 |
Cytomegalovirus detection by biotinylated DNA probes.
Topics: Antibodies, Monoclonal; Biotin; Cells, Cultured; Cytomegalovirus; Cytomegalovirus Infections; Cytopathogenic Effect, Viral; DNA Probes; Evaluation Studies as Topic; Fluorescent Antibody Technique; Humans; Immunoenzyme Techniques; Microbial Sensitivity Tests; Reagent Kits, Diagnostic; Sensitivity and Specificity | 1989 |
Detection of cytomegalovirus DNA using probes labelled with digoxigenin.
Topics: Biotin; Complement Fixation Tests; Cytomegalovirus; Cytomegalovirus Infections; Digoxigenin; Digoxin; DNA Probes; DNA, Viral; Evaluation Studies as Topic; Humans; Sensitivity and Specificity | 1989 |
Improved sensitivity of detection by avidin-biotin complex (ABC) immunocytochemistry in Epstein-Barr virus serology.
Topics: Antibodies, Viral; Antigens, Viral; Avidin; Biotin; Capsid Proteins; Herpesvirus 4, Human; Humans; Immunoglobulin A; Immunohistochemistry; Nasopharyngeal Neoplasms; Sensitivity and Specificity; Tumor Cells, Cultured | 1989 |
Nonradioactive, photobiotin-labelled DNA probes for routine diagnosis of viroids in plant extracts.
Topics: Affinity Labels; Azides; Biotin; Buffers; Cloning, Molecular; Colorimetry; DNA Probes; Formaldehyde; Immunoblotting; Plant Extracts; Sensitivity and Specificity; Viroids | 1989 |
Detection of antibodies against hepatitis B core antigen using the avidin-biotin system.
Topics: Avidin; Biotin; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Hepatitis B; Hepatitis B Antibodies; Hepatitis B Core Antigens; Hepatitis B virus; Humans; Radioimmunoassay; Sensitivity and Specificity | 1989 |