betadex and Respiratory-Distress-Syndrome

Shuang-Qing-Cao (SQC) is a folk Chinese medicinal formula. The therapeutic effects of inclusion complexation of SQC extract in beta-cyclodextrin (SQC-beta-CD) against lipopolysaccharide (LPS)-induced acute lung injury (ALI) were studied in mice.. Two protocols were designed for administration of SQC-beta-CD (10 and 20 mg/kg body weight) or DEX (2 mg/kg). According to Protocol A we intraperitoneally injected diluent (saline with 0.5% Tween 80), SQC-beta-CD or DEX respectively into mice 30 min and 3h after LPS challenge. Alternatively, in Protocol B we administered diluent, SQC-beta-CD or DEX 3h before and 30 min after LPS challenge.. The histological results showed that SQC-beta-CD (20 mg/kg) protected mice from LPS-induced ALI such as oedema, haemorrhage, blood vessel and alveolar structural damage. Furthermore, SQC-beta-CD inhibited LPS-increased pulmonary MPO activity and migration of polymorphonuclear neutrophils (PMNs) into bronchoalveolar lavage fluid (BALF). Immunohistochemical experiment demonstrated that SQC-beta-CD decreased inducible nitric oxide synthase (iNOS) expression in lung 24h after LPS administration. Consequently, SQC-beta-CD prevented LPS-induced nitric oxide (NO) release in BALF.. The results indicated that SQC-beta-CD is greatly effective in inhibiting ALI. The present study indicated that SQC-beta-CD acted as a potential therapeutic reagent for treating ALI.

Topics: Animals; Anti-Inflammatory Agents; beta-Cyclodextrins; Dexamethasone; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; Lipopolysaccharides; Male; Mice; Mice, Inbred ICR; Nitric Oxide; Nitric Oxide Synthase Type II; Plant Extracts; Respiratory Distress Syndrome; Time Factors

Topics: Acetates; Aged; AIDS Vaccines; American Medical Association; Amines; Animals; Anticonvulsants; beta-Cyclodextrins; Cells, Cultured; Clinical Trials as Topic; Cochlear Implants; Complementary Therapies; Constriction, Pathologic; Coronary Artery Bypass; Cyclodextrins; Cyclohexanecarboxylic Acids; Depressive Disorder; Drug Therapy, Combination; Epilepsy; Extracorporeal Membrane Oxygenation; Felbamate; Fetus; Gabapentin; gamma-Aminobutyric Acid; Genetic Therapy; Health Care Costs; HIV Infections; Humans; Hyperlipoproteinemia Type II; Lamotrigine; Muscle, Smooth; National Institutes of Health (U.S.); Oxygen; Phenylcarbamates; Propylene Glycols; Respiration, Artificial; Respiratory Distress Syndrome; Triazines; United States; Vascular Surgical Procedures