betadex and Lyme-Disease

Cyclodextrin (CD) is a kind of cyclic oligosaccharides, which forms host-guest interactions with hydrophobic molecules and is widely applied in capillary electrophoresis and pharmaceutical engineering. In this study, we established a succinyl-β-CD modified gold biochip for improvement of seroimmunological detection sensitivity of Lyme disease. We found that the CD modified biochip platform presented a stronger affinity property for VlsE protein in conjugation with >0.000475 μg/mL of antigen immobilization concentration, which was sensitive enough for fluorescence based assay. Detection limit for anti-VlsE IgG antibody was 0.39 μg/mL. Specificity of VlsE assay on the succinyl-β-CD modified biochip was successfully confirmed by an immunological block assay. Furthermore, the correlation coefficient (R

Topics: Adolescent; Adult; Aged; Aged, 80 and over; Antibodies, Bacterial; Antigens, Bacterial; beta-Cyclodextrins; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Female; Gold; Humans; Immunoassay; Immunoglobulin G; Infant; Lyme Disease; Male; Middle Aged; Protein Array Analysis; Sensitivity and Specificity; Young Adult