betadex and Adenocarcinoma-of-Lung

betadex has been researched along with Adenocarcinoma-of-Lung* in 2 studies

Other Studies

2 other study(ies) available for betadex and Adenocarcinoma-of-Lung

ArticleYear
Possible enhancing mechanisms for gene transfer activity of glucuronylglucosyl-β-cyclodextrin/dendrimer conjugate.
    International journal of pharmaceutics, 2012, Apr-15, Volume: 426, Issue:1-2

    We previously reported that glucuronylglucosyl-β-cyclodextrin (GUG-β-CyD) conjugate with polyamidoamine starburst dendrimer (GUG-β-CDE conjugate) with the average degree of substitution (DS) of cyclodextrin (CyD) of 1.8 (GUG-β-CDE conjugate (DS 1.8)), showed remarkably higher gene transfer activity than α-CyD/dendrimer conjugate (α-CDE conjugate (DS 1.2)) and β-CyD/dendrimer conjugate (β-CDE conjugate (DS 1.3)) in vitro and in vivo. In this study, to clarify the enhancing mechanism for high gene transfer activity of GUG-β-CDE conjugate (DS 1.8), we investigated the physicochemical properties, cellular uptake, endosomal escape and nuclear translocation of the plasmid DNA (pDNA) complexes as well as pDNA release from the complexes. The particle size, ζ-potential and cellular uptake of GUG-β-CDE conjugate (DS 1.8)/pDNA complex were mostly comparable to those of α-CDE conjugate (DS 1.2) and β-CDE conjugate (DS 1.3). Meanwhile, GUG-β-CDE conjugate (DS 1.8)/pDNA complex was likely to have high endosomal escaping ability and nuclear localization ability in A549 and RAW264.7 cells. In addition, the pDNA condensation and decondensation abilities of GUG-β-CDE conjugate (DS 1.8) were lower and higher than that of α-CDE conjugate (DS 1.2) or β-CDE conjugate (DS 1.3), respectively. These results suggest that high gene transfer activity of GUG-β-CDE conjugate (DS 1.8) could be, at least in part, attributed to high endosomal escaping ability, nuclear localization ability and suitable pDNA release from its complex.

    Topics: Active Transport, Cell Nucleus; Adenocarcinoma; Adenocarcinoma of Lung; alpha-Cyclodextrins; Animals; beta-Cyclodextrins; Buffers; Cell Line, Tumor; Cell Nucleus; Dendrimers; DNA; Endocytosis; Endosomes; Humans; Hydrogen-Ion Concentration; Lung Neoplasms; Mice; Nucleic Acid Conformation; Particle Size; Transfection

2012
Molecular recognition and enhancement of aqueous solubility and bioactivity of CD437 by β-cyclodextrin.
    Bioorganic & medicinal chemistry letters, 2011, Jan-15, Volume: 21, Issue:2

    CD437 (6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carboxylic acid) is a novel synthetic retinoic acid derivative that has been shown to selectively induce apoptosis in human lung cancer cells. This compound, however, is limited in its application due to its low solubility in aqueous solutions. One technique for increasing the solubility and bioavailability of a cytotoxic agent is the formation of inclusion complexes with cyclodextrins. Herein, we report the formation and characterization of a 2:1 complex between β-cyclodextrin (β-CD) and CD437. It is shown that CD437 is a tight binder of β-CD with an overall association constant of 2.6±0.6×10(7)M(-2). In addition, we demonstrate (a) that β-CD-derived complexation enhances the aqueous solubility of CD437, and (b) that a significant increase in the toxicity of CD437 against a human lung adenocarcinoma cell line can be achieved by co-treatment with β-CD.

    Topics: Adenocarcinoma; Adenocarcinoma of Lung; Antineoplastic Agents; beta-Cyclodextrins; Cell Line, Tumor; Cell Proliferation; Humans; Lung Neoplasms; Models, Molecular; Retinoids; Solubility

2011