beta-hederin and Liver-Neoplasms

An antibody that specifically interacts with an antigen could be applied to an active targeting delivery system. In this study, CD147 antibody was coupled with α-hed chitosan nanoparticles (α-Hed-CS-NPs). α-Hed-CS-CD147-NPs were round and spherical in shape, with an average particle size of 148.23 ± 1.75 nm. The half-maximum inhibiting concentration (IC50) of α-Hed-CS-CD147-NPs in human liver cancer cell lines HepG2 and SMMC-7721 was lower than that of free α-Hed and α-Hed-CS-NPs. α-Hed-induced cell death was mainly triggered by apoptosis. The increase in intracellular accumulation of α-Hed-CS-CD147-NPs was also related to CD147-mediated internalization through the Caveolae-dependent pathway and lysosomal escape. The higher targeting antitumor efficacy of α-Hed-CS-CD147-NPs than that α-Hed-CS-NPs was attributed to its stronger fluorescence intensity in the tumor site in nude mice.

Topics: Animals; Annexin A5; Antibodies, Monoclonal; Antineoplastic Agents; Apoptosis; Basigin; Chitosan; Endocytosis; Flow Cytometry; Fluorescence; Hep G2 Cells; Humans; Imaging, Three-Dimensional; Intracellular Space; Liver Neoplasms; Mice, Nude; Microscopy, Confocal; Nanoparticles; Oleanolic Acid; Particle Size; Propidium; Saponins; Spectroscopy, Fourier Transform Infrared; Spectroscopy, Near-Infrared; Subcellular Fractions