beta-endorphin and Shock--Traumatic

Dynamic changes of immunoreactive beta-endorphin (ir-beta-EP) in perfusates collected from hypothalamic paraventricular nuclei at different times after burn, and the effects of intrahypothalamic paraventricular microinjection of beta-endorphin or its antiserum on various cardiac indices (MAP, dP/dtmax, Lvsp and HR) and survival time, were observed in anesthetized SD rats after third degree burn of 20% total body surface area. The results showed a significant increase of ir-beta-endorphin contents in the perfusate with the appearance of two peaks. According to the cardiac indices and mean survival time, the condition of the burned animals were improved by injection of anti-beta-endorphin serum, while injection of beta-endorphin did the reverse. The above results suggest that massive accumulation of beta-endorphin in the paraventricular nucleus appears to be one of the important factors detrimental to burn shock.

Topics: Animals; beta-Endorphin; Blood Pressure; Burns; Heart Rate; Immune Sera; Male; Microinjections; Paraventricular Hypothalamic Nucleus; Rats; Rats, Sprague-Dawley; Shock, Traumatic

Dynamic changes of contents of beta-endorphin, leu-enkephalin and oxytocin in plasma were determined in seventeen fracture patients with shock, fascial space syndromes, or infection postoperation. There was significant rise of contents in beta-endorphin and leu-enkephalin but not in oxytocin after trauma, Of those accompanied by infection postoperation, beta-endorphin and oxytocin remained high. The contents of beta-endorphin and enkephalin decreased gradually in non-infectious group, but were still higher than normal. The most obvious change was seen in beta-endorphin, suggesting that beta-endorphin plays a more important role in trauma. This was also confirmed in rat injury model.

Topics: Animals; beta-Endorphin; Enkephalin, Leucine; Fractures, Bone; Humans; Male; Multiple Trauma; Oxytocin; Rats; Rats, Sprague-Dawley; Shock, Traumatic

The experiments have been performed on 120 Wistar rats and 546 CBWH mice. Met-enkephalin (ME) and beta-endorphin (BE) levels were determined to increase in blood and mid-brain 3 hours later after soft tissue compression of pelvic extremities. 48 hours later after six-hour compression BE and ME level in blood was increased, BE concentration in blood was also increased, and enkephalins' content in brain and blood was decreased as against the control. Opioid peptides influence on the host compression period has been studied. D-agonists were established to decrease, but mu-agonists were established to increase mice survival in shock. Opioid peptides' significance in traumatic shock pathogenesis is being discussed.

Topics: Animals; beta-Endorphin; Enkephalin, Methionine; Mesencephalon; Mice; Mice, Inbred Strains; Rats; Rats, Wistar; Shock, Traumatic; Time Factors

Intraventricular injections of anti-beta-endorphin serum (8 microliters) at 0, 1, 2, 3 h after burn shock (20% body surface area, 100 degrees C, 20 s) in different group rats prolonged the survival time, delayed the decrease of mean arterial pressure (MAP) and heart rate, and postponed the abnormal changes of ECG. The effect was most prominent at 1 h and little at 3 h after burn.

Topics: Animals; beta-Endorphin; Blood Pressure; Burns; Electrocardiography; Heart Rate; Immune Sera; Injections, Intraventricular; Male; Rats; Rats, Inbred Strains; Shock, Traumatic; Time Factors