beta-carotene and Hemolysis

Anchusa azurea Mill. (AA) is a medicinal plant largely used traditionally in folk medicine in Algeria; it is locally named hamham. It is effective in the treatment of various diseases.. The aim of the present study is to determine the antioxidant, anti-inflammatory, and anti- hemolytic effects of phenolic fractions from Anchusa azurea Mill.. In this study, various extracts from Anchusa azurea Mill. (AA) using solvents with increasing polarity were prepared. The quantification of polyphenols and flavonoids was determined. The anti-radical activity of the different extracts was evaluated using DPPH and by measuring the inhibition of the oxidative degradation of β-carotene. The In-vitro antihemolytic effect of the plant extracts is determined (CrE, ChE, AcE, and AqE). For each extract, four concentrations were tested: 10.59, 21.18, 42.37, 84.74 μg/ml. Vitamin C is used as a standard. The free-radical attack was measured by measuring the HT. This plant has a strong pharmacological power, which supports its traditional medicinal use.

Topics: Animals; Anti-Inflammatory Agents; Antioxidants; beta Carotene; Boraginaceae; Flavonoids; Hemolysis; Mice; Plant Extracts

The aim of the present study was to prepare chitosan-PVA-silver nanoparticles (CS-AgNPs) through green method. Chitosan and PVA polymers acted as stabilizing agents. DLS and TEM analyses showed that CS-AgNPs were homogeneously dispersed in matrix with an average size of 190-200 nm. The CS-AgNPs were tested for their antioxidant and antibacterial properties and the results revealed that they exhibited higher antioxidant activity than CS powder. Moreover, CS-AgNPs were characterized by a low cytotoxicity effect at 5-200 μg/ml against Chinese Hamster Ovary (CHO-K1) cells. In addition, the prepared CS-Ag NPs were found to promote significantly the wound healing, as determined by the wound contraction ratio and histological examination. A significant improvement in wound healing progression and in oxidative stress damage were observed for CS, CS-PVA and CS-AgNPs-treated wound tissues, when compared to control and CICAFLORA®-treated groups. The wound healing effect could be attributed to the antibacterial and antioxidant synergy of AgNPs and CS. Results strongly support the possibility of using CS-AgNPs for wound care applications.

Topics: Animals; Anti-Bacterial Agents; Antioxidants; beta Carotene; Biphenyl Compounds; Chitosan; CHO Cells; Cricetinae; Cricetulus; Escherichia coli; Free Radical Scavengers; Free Radicals; Gels; Hemolysis; Humans; Iron; Linoleic Acid; Male; Metal Nanoparticles; Microbial Sensitivity Tests; Oxidative Stress; Oxygen; Picrates; Rats; Rats, Wistar; Silver; Silver Nitrate; Skin; Staphylococcus aureus; Wound Healing

The aim of this work was to evaluate the antioxidant and hemolytic activities as well as the in vivo wound healing performance of a novel polysaccharide (FWEP) extracted from fenugreek (Trigonella foenum-graecum) seeds. The antioxidant activity was evaluated in vivo and in vitro using various assays. Results showed that FWEP exhibited strong antioxidant activities but no hemolytic activity was observed towards bovine erythrocytes. The application of FWEP hydrogel on the wound site in a rat model enhanced significantly wound healing activity and accelerated the wound closure after 14days of wound induction. Histological examination also demonstrated fully re-epithelialized wound with a complete epidermal regeneration. Altogether, these evidences demonstrated that FWEP had strong wound healing potential presumably achieved through its antioxidant activities.

Topics: Animals; Antioxidants; beta Carotene; Biomarkers; Biphenyl Compounds; Bleeding Time; Body Weight; Hemolysis; Hydrogen Peroxide; Hydroxyproline; Iron; Male; Oxidation-Reduction; Oxidative Stress; Picrates; Polysaccharides; Rats; Rats, Wistar; Seeds; Skin; Skin Physiological Phenomena; Trigonella; Wound Healing

Traditionally, Pine has been used to treat oxidative and inflammatory disorders. The study was aimed to investigate biological potential of phytoconstituents of Pinus plant species: Pinus roxburghii, Pinus wallichiana and Pinus gerardiana using in-vitro antioxidant, anti-inflammatory and antimicrobial methods.. The hydro-alcoholic extraction of dried plant: stem bark was done and the antioxidant activity was evaluated using free radical scavenging methods for 1,1-diphenyl-2-picrylhydrazyl, (DPPH), nitric oxide and hydrogen peroxide radicals, reducing power assays, and total antioxidant capacity. Anti-inflammatory activity was carried out using albumin denaturation and HRBC membrane stabilization assays. Antimicrobial and antifungal activities were also conducted using agar well diffusion method.. The qualitative phytochemical analysis of hydro-alcoholic stem bark extracts of three plant species revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides, triterpenoids and saponins. Quantitative phytochemical analysis of plant extracts showed the presence of phenolics, flavonoids, tannins, beta-carotene and lycopene. Plant extracts of three pinus species showed significant antioxidant activity against DPPH, nitric oxide and H2O2 radicals. In in-vitro anti-inflammatory investigation, Pinus roxburghii exhibited highest protection against albumin denaturation 86.54 ± 1.85 whereas Pinus gerardiana showed 82.03 ± 2.67. Moreover, plant extracts were found to prevent the growth of microorganisms Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans showing promising antibacterial and antifungal activities againstCandida albicans.. The findings of the present study derived the rational for the therapeutic usage of Pinus which is a highly timber yielding plant from Himalayan region, against oxidative, inflammatory and microbial diseases.

Topics: Anti-Bacterial Agents; Anti-Inflammatory Agents, Non-Steroidal; Antifungal Agents; Antioxidants; beta Carotene; Carotenoids; Cell Membrane; Flavonoids; Free Radical Scavengers; Hemolysis; Humans; India; Lycopene; Medicine, Ayurvedic; Phenols; Phytochemicals; Pinus; Plant Extracts; Tannins

The characteristics, biological properties, and purification of sulfated polysaccharides extracted from squid (Loligo vulgaris) skin were investigated. Their chemical and physical characteristics were determined using X-ray diffraction and infrared spectroscopic analysis. Sulfated polysaccharides from squid skin (SPSS) contained 85.06% sugar, 2.54% protein, 1.87% ash, 8.07% sulfate, and 1.72% uronic acid. The antioxidant properties of SPSS were investigated based on DPPH radical-scavenging capacity (IC50 = 19.42 mg mL(-1)), hydrogen peroxide-scavenging activity (IC50 = 0.91 mg mL(-1)), and β-carotene bleaching inhibition (IC50 = 2.79 mg mL(-1)) assays. ACE-inhibitory activity of SPSS was also investigated (IC50 = 0.14 mg mL(-1)). Further antimicrobial activity assays indicated that SPSS exhibited marked inhibitory activity against the bacterial and fungal strains tested. Those polysaccharides did not display hemolytic activity towards bovine erythrocytes. Fractionation by DEAE-cellulose column chromatography showed three major absorbance peaks. Results of this study suggest that sulfated polysaccharides from squid skin are attractive sources of polysaccharides and promising candidates for future application as dietary ingredients.

Topics: Angiotensin-Converting Enzyme Inhibitors; Animals; Anti-Bacterial Agents; Antifungal Agents; Antioxidants; beta Carotene; Cattle; Chromatography, DEAE-Cellulose; Chromatography, Ion Exchange; Free Radical Scavengers; Hemolysis; Hydrogen Peroxide; Loligo; Microbial Sensitivity Tests; Peptidyl-Dipeptidase A; Polysaccharides; Skin; Spectroscopy, Fourier Transform Infrared; Sulfates; X-Ray Diffraction

In this study, the acetylcholinesterase inhibition and in vitro and in vivo antioxidant activities of Ganoderma lucidum grown on germinated brown rice (GLBR) were evaluated. In antioxidant assays in vitro, GLBR was found to have strong metal chelating activity, DPPH, ABTS, hydroxyl and superoxide radical scavenging activity. Cell-based antioxidant methods were used, including lipid peroxidation on brain homogenate and AAPH-induced erythrocyte haemolysis. In antioxidant assays in vivo, mice were administered with GLBR and this significantly enhanced the activities of antioxidant enzymes in the mice sera, livers and brains. The amount of total phenolic and flavonoid compounds were 43.14 mg GAE/g and 13.36 mg CE/g dry mass, respectively. GLBR also exhibited acetylcholinesterase inhibitory activity. In addition, HPLC analyses of GLBR extract revealed the presence of different phenolic compounds. These findings demonstrate the remarkable potential of GLBR extract as valuable source of antioxidants which exhibit interesting acetylcholinesterase inhibitory activity.

Topics: Acetylcholinesterase; Animals; Antioxidants; Ascorbic Acid; beta Carotene; Carotenoids; Catalase; Chelating Agents; Cholinesterase Inhibitors; Erythrocytes; Female; Flavonoids; Free Radical Scavengers; Glutathione; Hemolysis; Lipid Peroxidation; Lycopene; Mice; Oryza; Oxidation-Reduction; Phenols; Plant Extracts; Reishi; Superoxide Dismutase

In order to clarify the contribution of phenolic and enolic hydroxyl group to the antioxidant capacity of feruloylacetone, a model compound of half-curcumin, 6-(p-hydroxy-m-methoxyphenyl)-5-hexene-2,4-dione (FT), 6-(p-benzyloxy-m-methoxyphenyl)-5-hexene-2,4-dione (BMFT), 6-(m,p-dihydroxyphenyl)-5-hexene-2,4-dione (DDFT), 6-(p-hydroxy-m-methoxyphenyl)hexane-2,4-dione (DHFT), 6-(p-hydroxy-m-methoxyphenyl)-5-hexene-2,4-diol (THFT), and ethyl 2-(p-hydroxy-m-methoxybenzylidene)-3-oxobutanoate (EOFT) were synthesized. The radical-scavenging abilities of these compounds were tested by trapping 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) cationic radical (ABTS(+·)), 2,2'-diphenyl-1-picrylhydrazyl (DPPH), and galvinoxyl radicals. The reductive capacities were screened by quenching singlet oxygen and by inhibiting the oxidation of linoleic acid. They were also employed to inhibit the oxidation of DNA mediated by hydroxyl radical and 2,2'-azobis(2-amidinopropane hydrochloride) (AAPH). In addition, they were applied to protect erythrocytes against AAPH- and hemin-induced hemolysis. The obtained results revealed that the antioxidant capacity of half-curcumin was derived from the phenolic-OH and the conjugated linkage between phenolic and enolic-OH. The enolic-OH itself cannot trap radicals.

Topics: Acetone; Amidines; beta Carotene; Copper; Curcumin; DNA; Erythrocytes; Free Radical Scavengers; Free Radicals; Glutathione; Hemin; Hemolysis; Humans; Linoleic Acids; Oxidation-Reduction; Structure-Activity Relationship; Styrenes

The antioxidant properties of different almond cultivars (cv.), either regional (Casanova, Duro Italiano, Molar, Orelha de Mula and Pegarinhos cv.) or commercial (Ferraduel, Ferranhês, Ferrastar and Guara cv.) were evaluated through several chemical and biochemical assays: DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of beta-carotene bleaching, inhibition of oxidative hemolysis in erythrocytes, induced by 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH), and inhibition of thiobarbituric acid reactive substances (TBARS) formation in brain cells, all used as models for the lipid peroxidation damage in biomembranes. The EC50 values were calculated for all the methods in order to evaluate the antioxidant efficiency of each almond cultivar. Bioactive compounds such as phenols and flavonoids were also obtained and correlated to antioxidant activity. The results obtained were quite heterogeneous, revealing significant differences among the cultivars assayed. Duro Italiano cv. revealed better antioxidant properties, presenting lower EC50 values in all assays, and the highest antioxidants contents. The protective effect of this cultivar on erythrocyte biomembrane hemolysis was maintained during 4h.

Topics: Amidines; Animals; Antioxidants; beta Carotene; Biphenyl Compounds; Brain; Brain Chemistry; Erythrocytes; Free Radical Scavengers; Hemolysis; Male; Oxidation-Reduction; Oxidative Stress; Picrates; Portugal; Prunus; Sheep; Swine; Thiobarbituric Acid Reactive Substances

Assessment of the antioxidant activity of vitamins and other compounds is of interest in the understanding of their in vivo effects. In this study, we have investigated the activity of several lipid and water-soluble vitamins in human whole blood. Measurements were carried out using a biological test that enables the evaluation of both red blood cells and plasma resistance against free radical activity induced by 2,2'-azobis (2-amidinopropane)hydrochloride (AAPH). Antioxidant activity of vitamins has been determined by using the biological test versus chemical methods (chemiluminescence, DMPD radical). We have observed strong anti-oxidant potentials for vitamins B6 and B9 with biological tests, but not with chemical methods. At 10 microM, the vitamin B9 efficiency in inhibiting radical-induced red blood cell hemolysis was almost three times higher than vitamin C efficiency and two times higher than alpha-tocopherol efficiency. Antioxidant activity was not observed for vitamins B1 or B2, nor for retinol. The weak activity of beta-carotene still remains to be investigated particularly in relation to oxygen pressure. Our study demonstrated that the biological test is more useful than the chemical methods employed in this instance, for the evaluation of antioxidant capacity of lipophilic and putatively biologically active compounds.

Topics: alpha-Tocopherol; Amidines; Antioxidants; Ascorbic Acid; beta Carotene; Biological Assay; Caffeic Acids; Dose-Response Relationship, Drug; Fluorescent Dyes; Hemolysis; Humans; Luminescent Measurements; Oxidants; Phenylenediamines; Reference Values; Solubility; Vitamin A; Vitamin B Complex; Vitamins

Carotenoids are a naturally occurring group of compounds that possess antioxidant properties. Most natural carotenoids display poor aqueous solubility and tend to form aggregates in solution. Disodium disuccinate astaxanthin (DDA; Cardax) is a water-dispersible synthetic carotenoid that rapidly and preferentially associates with serum albumin, thereby preventing the formation of supramolecular complexes and facilitating its efficacy after parenteral administration. This study investigated the ability of DDA to reduce inflammation and myocardial injury in a rabbit model of ischemia/reperfusion. DDA (50 mg/kg/day) or saline was administered i.v. for 4 consecutive days before the initiation of the protocol for induction of myocardial ischemia/reperfusion. On the 5th day, rabbits underwent 30 min of coronary artery occlusion, followed by a 3-h reperfusion period. Myocardial infarct size, as a percentage of the area at risk, was calculated for both groups. Infarct size was 52.5 +/- 7.5% in the vehicle-treated (n = 9) and 25.8 +/- 4.7% in the DDA-treated (n = 9) animals (p < 0.01 versus vehicle; mean myocardial salvage = 51%). To evaluate the anti-inflammatory effects of DDA, complement activity was assessed at the end of reperfusion using a red blood cell lysis assay. DDA administration significantly reduced (p < 0.01) the activation of the complement system in the serum. The current results, coupled with the well established antioxidant ability of carotenoids, suggest that the mechanism(s) of action by which DDA reduces the tissue damage associated with reperfusion injury may include both antioxidant and anticomplement components.

Topics: Adjuvants, Immunologic; Animals; beta Carotene; C-Reactive Protein; Complement Activation; Complement Inactivator Proteins; Complement Membrane Attack Complex; Erythrocytes; Fluorescent Antibody Technique; Hemodynamics; Hemolysis; Male; Myocardial Infarction; Myocardial Reperfusion Injury; Myocardium; Rabbits; Tetrazolium Salts; Thiazoles; Tissue Distribution; Troponin I; Xanthophylls

This study was carried out to investigate sequel of oxidative insult to human erythrocytes induced by a water-soluble radical initiator, 2,2'-azobis-(amidinopropane) dihydrochloride (AAPH) and the effect of a commercially available mixed antioxidant (Blackmores, BioAce Excel), containing alpha-tocopherol, ascorbic acid, beta-carotene and some herbal extracts (containing grape seed catechins and milk thistle derived silybin), on lipid peroxidation, degradation of membrane proteins and haemolysis. We performed this study in order firstly to clarify aspects of the mechanism of AAPH induced free radical damage in human erythrocytes and secondly to establish in vitro conditions by which the efficacy of mixed antioxidant preparations may fairly and objectively be compared. In the process of oxidation initiated by peroxyl radical, a rapid loss of reduced glutathione occurred in the first 60 min. Formation of thiobarbitric acid-reactive substances indicative of lipid peroxidation increased subsequently and almost reached maximal levels at 180 min before significant apparent degradation of membrane proteins was detected. At this point, a significant haemolysis occurred. This sequence of events is consistent with the idea that haemolysis is a consequence of lipid peroxidation and the degradation of membrane proteins. The mixed commercial antioxidant, which suppressed lipid peroxidation and protected membrane proteins against degradation induced by peroxyl radicals, also effectively delayed AAPH induced haemolysis. The system we describe provides a sound objective basis for the in vitro comparison of the potential efficacy of the hundreds of antioxidant nutritional supplements currently available in the market place.

Topics: Amidines; Antioxidants; Ascorbic Acid; beta Carotene; Catechin; Drug Combinations; Erythrocytes; Fruit; Glutathione; Hemolysis; Humans; In Vitro Techniques; Kinetics; Lipid Peroxidation; Membrane Proteins; Methemoglobin; Oxidants; Plant Extracts; Seeds; Silymarin; Thiobarbituric Acid Reactive Substances; Vitamin E

The present study was designed to investigate the effect of antioxidant supplementation on the in vitro osmotic fragility of erythrocytes from zinc-deficient rats. Rats were fed either a zinc-adequate diet, zinc-deficient diet or a zinc-deficient diet enriched either with vitamin C or vitamin E or beta-carotene. Components of the primary antioxidant system of erythrocytes, parameters of hemolysis in vivo and indicators of liver injuries were also examined. In order to ensure adequate and identical food intake rats were force-fed by intragastric tube. The supplementation with antioxidants led to a marked improvement of the osmotic fragility without having influenced zinc status of the animals and components of the antioxidant system. The strongest effect was exerted by vitamin E. The rats fed the zinc-adequate diet (control group) showed unusually high values of erythrocytes osmotic fragility. Therefore there was no difference between control group and zinc-deficient group. A possible reason for this is discussed. Zinc deficiency led to a reduction of serum zinc concentration and alkaline phosphatase activity as well as to changes in the antioxidant system of erythrocytes characterized by a decrease of glutathione and an increase of glutathione S-transferase activity. Superoxide dismutase activity in serum decreased. There was no indication for hemolysis in vivo and for liver injuries.

Topics: Alkaline Phosphatase; Analysis of Variance; Animals; Antioxidants; Ascorbic Acid; beta Carotene; Eating; Erythrocytes; Food, Fortified; Glutathione Transferase; Hemolysis; Male; Models, Statistical; Osmotic Fragility; Rats; Rats, Sprague-Dawley; Superoxide Dismutase; Vitamin E; Zinc

Substantial evidence supports the theory that free radicals, especially oxygen radicals, are involved in the process of aging. The human organisms have two ways to fight them: an enzymatic way with enzymatic intervention like superoxide dismutase, catalase... and a chemical way with the intervention of scavengers such as vitamins, cysteine, methionine, gluthatione... The aim of this work was to determine that an intakes of vitamins association: vitamin E, vitamin C and beta carotene induce an increase of singlet oxygen protection of erythrocytes' subjects. The method was based on the haemolytic effect of singlet oxygen which is generated by irradiation of hematoporphyrine at 365 nm, in 22 p. cent suspension of erythrocytes' subjects. Results show that a supply of beta carotene (15 or 30 mg/day), vitamin E (15 mg/day) and vitamin C (30 mg/day) involves an increase of singlet oxygen protection of erythrocytes of subjects. This protection appears very quickly after 15 days of treatment.

Topics: Administration, Oral; Ascorbic Acid; beta Carotene; Carotenoids; Erythrocyte Aging; Erythrocytes; Hematoporphyrins; Hemolysis; Humans; Oxygen; Photochemistry; Reference Values; Singlet Oxygen; Ultraviolet Rays; Vitamin E

The sensitivity of human erythrocytes to photohemolysis sensitized by addition of protoporphyrin IX can be selectively affected by their enrichment with substances carried by cationic liposomes. In particular the enrichment which superoxide dismutase is accompanied by a copper-related greater sensitivity toward photohemolysis, as observed in the Down's syndrome (mongolism). Instead it is possible to protect the erythrocytes against the phototoxic effect of protoporphyrin by enrichment with small amounts of beta-carotene.

Topics: Animals; beta Carotene; Carotenoids; Cattle; Down Syndrome; Erythrocytes; Hemolysis; Humans; Kinetics; Light; Liposomes; Protoporphyrins; Superoxide Dismutase