beta-carotene and Growth-Disorders

Biofortified maize, designed as an intervention strategy to prevent vitamin A deficiency, can provide upwards of 15 μg β-carotene per g dry weight. Some varieties also have elevated concentrations of other carotenoids. We conducted a cluster randomized, controlled feeding trial in rural Zambia to test the impact of daily consumption of biofortified maize over a 6-month period on vitamin A status. Serum concentrations of retinol and carotenoids were assessed by high-performance liquid chromatography. Data on circulating carotenoids by intervention group in 679 children are reported here. As previously shown, consumption of this β-carotene-rich maize significantly improved serum β-carotene concentrations (0.273 vs. 0.147 μmol/L, p < 0.001, in this subset of children). Here we show significant increases in α-carotene, β-cryptoxanthin, and zeaxanthin (p < 0.001). There was no impact on lutein or lycopene concentrations. Consumption of biofortified maize can have broader implications beyond the control of vitamin A deficiency (Trial registration: NCT01695148).

Topics: beta Carotene; Beta-Cryptoxanthin; Carotenoids; Child; Child, Preschool; Diet; Female; Food, Fortified; Growth Disorders; Humans; Lutein; Male; Nutritional Status; Socioeconomic Factors; Thinness; Zambia; Zea mays; Zeaxanthins

The role of oxidative stress is well known in the pathogenesis of acquired malnutrition. Intrauterine growth restriction has been associated with an imbalance in oxidative stress/antioxidant system. Therefore, early postnatal environment and, consequently, extrauterine growth restriction might be associated with alterations in the antioxidant defense system, even in the prepubertal stage.. This is a descriptive, analytical, and observational case-control study. The study included two groups; 38 Caucasian prepubertal children born prematurely and with a history of extrauterine growth restriction as the case group, and 123 gender- and age-matched controls. Plasma exogenous antioxidant (retinol, β-carotene, and α-tocopherol) concentrations were measured by HPLC; antioxidant enzyme activities of catalase, glutathione reductase, glutathione peroxidase, and superoxide dismutase were determined in lysed erythrocytes by spectrophotometric techniques.. Catalase and glutathione peroxidase concentrations were significantly lower in extrauterine growth restriction children than in controls (P < 0.001). Lower plasma retinol concentrations were found in the case group (P = 0.029), while concentrations of β-carotene and α-tocopherol were higher (P < 0.001) in extrauterine growth restriction prepubertal children as compared with controls. After correction by gestational age, birth weight, and length, statistically significant differences were also found, except for retinol.. Prepubertal children with a history of extrauterine growth restriction present alterations in their antioxidant defense system. Knowing these alterations may be important in establishing pharmacological and nutritional treatments as this situation might be associated with higher metabolic disorders in adulthood.

Topics: alpha-Tocopherol; Antioxidants; beta Carotene; Biomarkers; Birth Weight; Case-Control Studies; Catalase; Child; Child, Preschool; Erythrocytes; Female; Gestational Age; Glutathione Peroxidase; Glutathione Reductase; Growth Disorders; Humans; Infant, Newborn; Infant, Premature; Male; Nutritional Status; Oxidative Stress; Superoxide Dismutase; Vitamin A

Growth and pubertal development in hemophilic males, age 6-19 years at baseline, were evaluated over a 3.5-year period in 207 HIV-positive and 126 HIV-negative subjects as part of the Hemophilia Growth and Development Study.. Thyroid function, insulin-like growth factor I (IGF-1) levels, bone age, cranial magnetic resonance image normality, CD4+ counts, and serum testosterone levels of study participants were measured at baseline. An extensive endocrine evaluation was performed in subjects who demonstrated declines in height for age (measurement <5th percentile with two pervious heights >10th percentile), who had not achieved Tanner stage 4 level of pubertal development by age 15 years or who had abnormal growth velocity, which included assessment of peak stimulated growth hormone response after clonidine stimulation, 12-hour growth hormone profiles, and serum beta carotene levels (triggered protocol).. For almost the entire group (-99%), thyroid function tests were normal for age. IGF-1 levels were normal for 93% of the cohort. A total of 120 subjects, 89 HIV-positive and 31 HIV-negative, had an abnormality of growth, pubertal development, or both; 34 (11.1%) HIV-positive and 4 (3.6%) HIV-negative subjects had declines in height (p = .001), 20 (23.3%) HIV-positive and 5 (15.8%) HIV-negative subjects had not achieved Tanner stage 4 by 15 years of age (p = .372) and 59 (43.4%) HIV-positive and 23 (25.6) HIV-negative subjects had abnormal growth velocity (p < 0.001). Among subjects with abnormal height or growth velocity, the HIV-positive group had significantly lower mean age-adjusted testosterone levels than did the HIV-negative group (p = .030). Within the HIV-positive group, older subjects with abnormal height or growth velocity had significantly lower mean bone age than subjects of similar age without growth abnormalities (p = .0092). Extensive testing was done in 39 patients (32 HIV-positive, 7 HIV-negative). Half of the HIV-positive subjects had mean 12-hour growth hormone levels <3 ng/ml, 47% had peak stimulated levels <10 ng/ml, 28% had peak spontaneous values <10 ng/ml, and 38% had low levels of IGF-1. In the HIV-positive cohort, there was no difference in the rate of abnormalities of growth hormone secretion between those with CD4+ counts > or = or <200 cells/mm3 and between those subjects that met the 1987 Centers for Disease Control (CDC) surveillance definition of AIDS. In the subset of HIV-positive patients with abnormal peak growth hormone levels after clonidine stimulation, growth hormone response correlated positively with CD4+ count (r = .657, p = .0056) and beta carotene concentration (R = .596, p = .0192).. The results of this longitudinal study suggest that abnormalities of growth and pubertal development, particularly an abnormal growth velocity, are common in HIV-infected hemophilic boys and adolescents. These abnormalities might serve as indicators of the presence of HIV infection in this at-risk population. Since thyroid function tests and IGF-1 levels were normal, the etiology of growth impairment in HIV infection does not appear to be secondary to inadequate caloric intake or acquisition, or severe illness such as that caused by recurrent or persistent infection. Rather, HIV infection appears to lead to diminished growth hormone production or release and decreased androgen secretion, even before the development of AIDS and immunocompromise. These results provide a rationale for trials of treatment with growth hormone or androgens in patients with abnormalities of endocrine function.

Topics: Adolescent; Adult; Age Determination by Skeleton; beta Carotene; Body Height; CD4 Lymphocyte Count; Child; Growth Disorders; Hemophilia A; HIV Seronegativity; HIV Seropositivity; Human Growth Hormone; Humans; Insulin-Like Growth Factor I; Longitudinal Studies; Magnetic Resonance Imaging; Male; Puberty, Delayed; Skull; Testosterone; Thyroid Gland

A possible involvement of vitamin A in regulating physiological nocturnal growth hormone secretion has been recently suggested leading us to evaluate the daily vitamin A supply in prepubertal school children.. A questionnaire including a list of foods containing vitamin A and/or beta-carotene was answered with the aid of the parents. Vitamin A supply was expressed in retinol-Equivalent and estimated as mean daily intake over a one-year period. Following this methodology, a study was conducted in 104 control school children with normal stature and 110 children with short stature.. The total daily vitamin A intake (mean +/- SD: 1.197 micrograms +/- 799), retinol (675 micrograms +/- 628) and beta carotene (525 micrograms +/- 355) was above or equal to the recommended intake in more than 75% of the control children. In contrast, the total daily vitamin A intake (mean +/- SD: 787 micrograms +/- 850, P < 0.0002) retinol (436 micrograms +/- 670, P < 0.0004) and beta carotene (353 micrograms +/- 466, P < 0.002) was significantly decreased in those children with short stature, more than 35% of them having daily intake below the recommended one. The dietary vitamin A intake was also deficient when expressed as ER/1,000 calories (mean SD = 444 +/- 262) in the 46 children with short stature in whom the calorie intake had been evaluated for three days.. This study confirms that annual dietary vitamin A intake can easily be measured in school children. Its results suggest that this intake, relatively deficient in children with short stature, could be correlated with deficient secretion of growth-hormone.

Topics: beta Carotene; Child; Child, Preschool; Dietary Proteins; Energy Intake; Female; Growth Disorders; Humans; Male; Surveys and Questionnaires; Vitamin A