benzoporphyrin-d has been researched along with Leukemia* in 2 studies
2 other study(ies) available for benzoporphyrin-d and Leukemia
| Article | Year |
|---|---|
Inhibition of endocytic processes by photodynamic therapy.
Recent studies have demonstrated an effect of photodamage on the endocytic pathway involved in recycling of membrane components. Using a series of agents with known sub-cellular targets, we explored the determinants of photodynamic inhibition of endocytic processes in three cell lines: A murine leukemia, a murine hepatoma, and a non-malignant epithelial cell line of human origin.. The PI-3 kinase antagonist wortmannin blocks endosomal processing pathway dependent on this enzyme, providing an indication of the "flux" of endocytosis. Microscopic observations were used to assess the effect of photodamage on this pathway. Photosensitizing agents specific for mitochondrial, endoplasmic reticulum (ER), lysosomal, and endosomal photodamage were employed.. Sub-lethal photodamage directed against endosomes or lysosomes interrupted early steps in this endocytic process in the hepatoma cell line. A mechanism for these effects is proposed. Mitochondrial photodamage could interrupt endocytosis, but at levels that also induced apoptosis. ER photodamage did not affect endocytosis even at lethal levels. Somewhat similar results were obtained with other cell lines, but there were sufficient differences to indicate that the cell phenotype is, in part, a determinant of the endocytic response to PDT.. PDT is therefore seen to have an effect on endocytic processes. Further work will be needed to delineate the role of these endocytic effects in the array of responses to photodynamic therapy. Topics: Androstadienes; Animals; Carcinoma, Hepatocellular; Cell Line; Cell Line, Tumor; Endocytosis; Epithelial Cells; Humans; Indoles; Leukemia; Liver Neoplasms; Mesoporphyrins; Mice; Models, Biological; Organelles; Organometallic Compounds; Phosphodiesterase Inhibitors; Photochemotherapy; Photosensitizing Agents; Porphyrins; Wortmannin | 2011 |
Preferential uptake of benzoporphyrin derivative by leukemic versus normal cells.
Benzoporphyrin derivatives (BPDs) are photosensitizers, which fluoresce strongly at 690 nm, and may be candidates for various applications of photodynamic therapy (PDT). Fluorescence-activated cell sorting (FACS) analysis, subsequent to ultraviolet light excitation, revealed pronounced differences in red fluorescence between leukemic cell lines (HL60, K562 and L1210), leukemic clinical isolates, and normal human or murine bone marrow cells incubated with BPD. These observed differences in BPD-mediated fluorescence provide the rationale for sorting leukemic from normal cells via FACS or may constitute a novel method for extracorporeal purging of remission marrow by photodynamic therapy in autologous bone marrow transplantation. Topics: Animals; Biological Transport; Bone Marrow; Cell Line; Female; Flow Cytometry; Humans; In Vitro Techniques; Kinetics; Leukemia; Leukemia L1210; Mice; Mice, Inbred DBA; Monocytes; Porphyrins; Radiation-Sensitizing Agents; Reference Values; Spleen; Structure-Activity Relationship; Tumor Cells, Cultured | 1990 |