benzofurans and Idiopathic-Pulmonary-Fibrosis

Alveolar epithelial cell (AEC) senescence is implicated in the pathogenesis of idiopathic pulmonary fibrosis (IPF). Mitochondrial dysfunction including release of mitochondrial DNA (mtDNA) is a feature of senescence, which led us to investigate the role of the DNA-sensing guanine monophosphate-adenine monophosphate (GMP-AMP) synthase (cGAS) in IPF, with a focus on AEC senescence. cGAS expression in fibrotic tissue from lungs of patients with IPF was detected within cells immunoreactive for epithelial cell adhesion molecule (EpCAM) and p21, epithelial and senescence markers, respectively. Submerged primary cultures of AECs isolated from lung tissue of patients with IPF (IPF-AECs,

Topics: A549 Cells; Alveolar Epithelial Cells; Benzofurans; Cell Line, Tumor; Cellular Senescence; Cyclin-Dependent Kinase Inhibitor p21; Cytokines; Deoxyribonuclease I; DNA Damage; DNA, Mitochondrial; Epithelial Cell Adhesion Molecule; Etoposide; Humans; Idiopathic Pulmonary Fibrosis; Mitochondria; Nucleotidyltransferases; RNA Interference; RNA, Small Interfering; Signal Transduction