benzofurans and Dermatitis--Allergic-Contact

An immunosuppressant with fewer adverse effects is still urgently needed for increasing numbers of patients suffering from allergic contact dermatitis. In the present study, we aimed to investigate the immunosuppressive activity of herpetol on T-lymphocytes in vitro and in vivo and explore its potential pharmacological mechanism. The results showed that herpetol could effectively inhibit the proliferation of activated T cells and reduce the production of pro-inflammatory cytokines at 5-20μM. Additionally, the ear swelling and inflammatory responses induced by picryl chloride were significantly ameliorated by herpetol at 20-40mg/kg. Moreover, herpetol could cause cell cycle arrest of activated T cells in a dose-dependent manner. Furthermore, herpetol reduced the expression and activity of HIF-1α, Glut1 and LDHA, leading to glycolysis inhibition in activated T cells. Taken together, herpetol showed an immunosuppressive activity against T-cell mediated immune responses in vitro and in vivo, and it has potential for the treatment of immune-related skin diseases.

Topics: Animals; Benzofurans; Cell Cycle; Dermatitis, Allergic Contact; Ear; Female; Glucose Transporter Type 1; Glycolysis; Hypoxia-Inducible Factor 1, alpha Subunit; Immunosuppressive Agents; Lymph Nodes; Mice, Inbred BALB C; Mice, Inbred C57BL; Picryl Chloride; Spleen; T-Lymphocytes

Several types of pesticides, such as organophosphates, phenoxyacetic acid, and carbamate have a high risk of affecting human health, causing allergic rhinitis and bronchial asthma-like diseases. We used our long-term sensitization method and a local lymph node assay to examine the allergic reactions caused by several types of pesticides. BALB/c mice were topically sensitized (9 times in 3 weeks), then challenged dermally or intratracheally with 2,4-D, BRP, or furathiocarb. One day post-challenge, the mice were processed to obtain biologic materials for use in assays of total IgE levels in serum and bronchoalveolar lavage fluid (BALF); differential cell counts and chemokine levels in BALF; lymphocyte counts and surface antigen expression on B-cells within regional lymph nodes (LNs); and, ex situ cytokine production by cells from these LNs. 2,4-D-induced immune responses characteristic of immediate-type respiratory reactions, as evidenced by increased total IgE levels in both serum and BALF; an influx of eosinophils, neutrophils, and chemokines (MCP-1, eotaxin, and MIP-1beta) in BALF; increased surface antigen expression on B-cells IgE and MHC class II production) in both auricular and the lung-associated LNs; and increased Th2 cytokine production (IL-4, IL-5, IL-10, and IL-13) in both auricular and the lung-associated LN cells. In contrast, BRP and furathiocarb treatment yielded, at most, non-significant increases in all respiratory allergic parameters. BRP and furathiocarb induced marked proliferation of MHC Class II-positive B-cells and Th1 cytokines (IL-2, TNF-alpha, and IFN-gamma) in only auricular LN cells. These results suggest that 2,4-D is a respiratory allergen and BRP and furathiocarb are contact allergens. As our protocol detected classified allergic responses to low-molecular-weight chemicals, it thus may be useful for detecting environmental chemical-related allergy.

Topics: 2,4-Dichlorophenoxyacetic Acid; Administration, Cutaneous; Administration, Inhalation; Allergens; Animals; Benzofurans; Bronchoalveolar Lavage Fluid; Carbamates; Cell Proliferation; Chemokines; Cytokines; Dermatitis, Allergic Contact; Dose-Response Relationship, Drug; Female; Immunoglobulin E; Leukocyte Count; Local Lymph Node Assay; Lymph Nodes; Lymphocyte Activation; Male; Mice; Mice, Inbred BALB C; Mice, Inbred CBA; Organophosphorus Compounds; Pesticides; Respiratory Hypersensitivity

Botanical ingredients used in personal care products are a significant and underreported cause of allergic contact dermatitis.. To evaluate allergic contact dermatitis from a widely-used botanical deodorant.. We conducted patch testing in four patients who were using the botanical deodorant and were referred to the contact dermatitis clinic; three patients had axillary dermatitis and one had dermatitis of the external ear.. All four patients had positive patch test reactions to lichen acid mix and D-usnic acid. Of the three patients who were patch tested to the botanical deodorant, all had positive reactions.. We did not test to the specific lichen used in the natural deodorant but rather used our own lichen acid mix and d-usnic acid in addition to testing to the actual product. One of the patients declined to be tested with the natural deodorant, but did test positive to the lichen acid mix and d-usnic acid.. Personal care products such as deodorants may represent a new route of exposure to lichen extract, a known allergen.

Topics: Adult; Antifungal Agents; Benzofurans; Deodorants; Dermatitis, Allergic Contact; Humans; Lichens; Male; Middle Aged; Plant Extracts

A 48-year-old clerical officer with a recurrent facial eruption had positive patch test reactions to nickel, fragrance mix and lichen acid mix. On testing to individual ingredients of fragrance mix and lichen acid mix, she had 2+ reactions to oak moss, which is thought to be the main allergen in fragrance mix, and to usnic acid, which is one of a number of lichen acids comprising oak moss. Avoidance of fragrance use resulted in clearing of the eruption but, subsequently, an acute vesicular flare on her face and hands occurred after exposure to lichen on garden shrubs.

Topics: Allergens; Benzofurans; Dermatitis, Allergic Contact; Facial Dermatoses; Female; Humans; Hydroxybenzoates; Lichens; Middle Aged; Patch Tests; Perfume

Topics: Adult; Allergens; Benzofurans; Dermatitis, Allergic Contact; Dermatitis, Occupational; Eczema; Humans; Irritants; Male; Plants; Sesquiterpenes

Topics: Acetophenones; Adult; Benzofurans; Chemical Industry; Dermatitis, Allergic Contact; Dermatitis, Occupational; Eczema; Facial Dermatoses; Forearm; Hand Dermatoses; Humans; Male

Topics: Administration, Intravaginal; Adult; Antitrichomonal Agents; Benzofurans; Dermatitis, Allergic Contact; Female; Humans; Patch Tests

Topics: Benzofurans; Dermatitis, Allergic Contact; Dermatitis, Contact; Fungi; Humans; Hypersensitivity; Lichens; Occupational Diseases; Pathology