benzofurans has been researched along with Cerebellar-Diseases* in 2 studies
2 other study(ies) available for benzofurans and Cerebellar-Diseases
| Article | Year |
|---|---|
Potential usefulness of D2R reporter gene imaging by IBF as gene therapy monitoring for cerebellar neurodegenerative diseases.
We investigated a gene expression imaging method to examine the level of therapeutic gene expression in the cerebellum. Using a human immunodeficiency virus derived lentivial vector, we expressed the dopamine D(2) receptor (D(2)R) as a reporter protein to mouse cerebellar Purkinje cells. Biodistribution and ex vivo autoradiography studies were performed by giving [(125)I]5-iodo-7-N-[(1-ethyl-2-pyrrolidinyl)methyl]carboxamide-2,3-dihydrobenzofuran ([(125)I]IBF) (1.85 MBq), as a radioactive D(2)R ligand, to model mice expressing the D(2)R with an HA tag (HA-D(2)R) in the cerebellum. In this study, [(125)I]IBF was bound to the D(2)R expressed in the cerebellum of the model mice selectively. Immunostaining was performed to confirm the HA-D(2)R expression in the cerebellum of the model mice. A significant correlation (r=0.900, P<0.001) between areas that expressed HA-D(2)R by immunostaining and areas in which [(125)I]IBF accumulated by the ex vivo autoradiograms was found. These results indicated that radioiodinated IBF is useful as a reporter probe to detect D(2)R reporter gene expression, which can be used for monitoring therapeutic gene expression in the cerebellum. Topics: Animals; Benzofurans; Cerebellar Diseases; Gene Expression Regulation; Genes, Reporter; Genetic Therapy; Genetic Vectors; Lentivirus; Mice; Mice, Inbred C57BL; Neurodegenerative Diseases; Organic Chemicals; Pyrrolidines; Receptors, Dopamine D2 | 2009 |
[Neuropathy and cerebellar syndrome induced by amiodarone].
A 62 year old man developed a neuropathy after several months of treatment with amiodarone. The clinical picture was atypical in that it associated a polyradiculoneuritis with cell-protein dissociation and an axial and peripheral cerebellar syndrome. Pathology of muscle and nerve showed dense inclusions in Schwann cell cytoplasm and in pericytes, highly suggestive of fat inclusions. Discontinuation of amiodarone therapy resulted in a slow regression of disorders. Diabetes mellitus developed. Several pathogenic hypotheses are proposed. Topics: Amiodarone; Benzofurans; Cerebellar Diseases; Coronary Disease; Diabetes Mellitus; Humans; Lipids; Lysosomes; Male; Middle Aged; Muscles; Peripheral Nerves; Polyradiculoneuropathy; Schwann Cells | 1985 |